FastQCFastQC Report
Thu 26 May 2016
SRR938234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279722
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90600.39741687802284664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76750.33666385638248875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.2095869584098412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47350.051.5692981
GTATCAA78900.039.377561
ATCAACG97950.031.2792033
TCAACGC99450.030.9486754
TATCAAC101500.030.4693372
CAACGCA101200.030.4134945
AACGCAG104300.029.554446
GTGGTAT25650.025.9291761
ACGCAGA120300.025.544717
TGGTATC23900.025.243752
CGCAGAG120650.025.2344048
TACATGG72150.023.5060672
GTACATG74400.022.9229011
GAGTACT86450.022.41596812-13
GCAGAGT135700.022.2607389
ACATGGG74500.021.869473
GTGTAAG10500.021.264571
CAGAGTA125850.021.17249110-11
GTACTTT91400.021.04607814-15
GTAAGGT10100.020.6920344
Produced by FastQC (version 0.11.5)