##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938234_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2279722 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48499773217963 33.0 31.0 34.0 31.0 34.0 2 32.716488677128176 34.0 31.0 34.0 31.0 34.0 3 32.68270736519628 34.0 31.0 34.0 31.0 34.0 4 36.209547479912025 37.0 35.0 37.0 35.0 37.0 5 36.171740238502764 37.0 35.0 37.0 35.0 37.0 6 36.22913539457881 37.0 37.0 37.0 35.0 37.0 7 36.188964707100254 37.0 37.0 37.0 35.0 37.0 8 36.18563228323453 37.0 37.0 37.0 35.0 37.0 9 37.89097398717914 39.0 38.0 39.0 35.0 39.0 10-11 37.85209161467934 39.0 38.0 39.0 35.0 39.0 12-13 37.793382921250924 39.0 38.0 39.0 35.0 39.0 14-15 39.07514315341959 40.0 38.0 41.0 36.0 41.0 16-17 39.00981808308206 40.0 38.0 41.0 36.0 41.0 18-19 39.00133744377604 40.0 38.5 41.0 36.0 41.0 20-21 39.00084505917827 40.0 38.5 41.0 35.5 41.0 22-23 39.03105027718291 40.0 39.0 41.0 35.0 41.0 24-25 38.99082541643236 40.0 38.5 41.0 35.0 41.0 26-27 38.82292446184228 40.0 38.0 41.0 35.0 41.0 28-29 38.74245982624197 40.0 38.0 41.0 35.0 41.0 30-31 38.533637434739845 40.0 38.0 41.0 34.0 41.0 32-33 38.341620601108374 40.0 38.0 41.0 34.0 41.0 34-35 38.13727572923365 40.0 38.0 41.0 34.0 41.0 36-37 38.06566984921845 40.0 38.0 41.0 33.5 41.0 38-39 38.021088536233805 40.0 38.0 41.0 33.0 41.0 40-41 37.89943115871146 40.0 38.0 41.0 33.0 41.0 42-43 37.711994927451684 40.0 37.0 41.0 33.0 41.0 44-45 37.46788753190082 40.0 37.0 41.0 33.0 41.0 46-47 37.12866130168503 40.0 37.0 41.0 31.5 41.0 48-49 36.76602936673858 40.0 36.0 41.0 31.0 41.0 50-51 36.47310505403729 39.0 36.0 41.0 30.0 41.0 52-53 36.01307812970178 39.0 35.0 40.0 29.0 41.0 54-55 35.69566596277967 39.0 35.0 40.0 28.0 41.0 56-57 35.42484237113122 38.0 35.0 40.0 28.0 41.0 58-59 35.04225295891341 38.0 34.0 40.0 27.0 41.0 60-61 34.62907823848697 38.0 34.0 40.0 26.0 41.0 62-63 34.19145251043767 37.0 33.5 40.0 26.0 41.0 64-65 33.746308979779116 36.5 33.0 39.0 25.0 41.0 66-67 33.226427827603544 36.0 32.5 39.0 23.0 40.0 68-69 32.688517284125 35.0 32.0 38.5 22.0 40.0 70-71 32.09828720343972 35.0 31.0 38.0 20.5 40.0 72-73 31.61903666324227 35.0 31.0 37.0 19.0 39.0 74-75 31.056074161674097 34.5 30.0 36.5 17.5 39.0 76-77 29.86148990973461 33.5 29.0 35.0 17.0 37.5 78-79 30.557391208226264 34.0 30.0 36.0 17.0 38.0 80-81 30.357339184339143 34.0 30.0 35.5 12.0 37.0 82-83 29.889019801537202 34.0 30.0 35.0 7.0 37.0 84-85 29.366726513145025 34.0 29.0 35.0 4.0 36.0 86-87 28.815506671427478 34.0 29.0 35.0 2.0 36.0 88-89 28.184157980666065 33.0 27.5 35.0 2.0 35.5 90-91 27.638269710078685 33.0 26.5 35.0 2.0 35.0 92-93 26.994543194301762 33.0 25.5 35.0 2.0 35.0 94-95 26.23324137767675 32.0 24.5 34.0 2.0 35.0 96-97 25.405151812370104 32.0 21.5 34.0 2.0 35.0 98-99 24.373049652545355 31.5 10.0 34.0 2.0 35.0 100-101 20.877315962209426 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0027418353408847906 1101 2 -0.004425449397544412 1101 3 0.13579795573411957 1101 4 0.0348704264857389 1101 5 0.02746396340405255 1101 6 0.06920928959291217 1101 7 0.03680667746139932 1101 8 0.1290473703267878 1101 9 -0.002230881112375016 1101 10-11 0.11630665032097198 1101 12-13 0.09855167475728166 1101 14-15 0.129660598892535 1101 16-17 0.1008982740717741 1101 18-19 0.18574492008562515 1101 20-21 0.25074170432281306 1101 22-23 0.19973791104023064 1101 24-25 0.12509070396090038 1101 26-27 0.186716939164711 1101 28-29 0.12037673625405887 1101 30-31 0.1388242666974051 1101 32-33 0.2138333279971789 1101 34-35 0.04443990964285405 1101 36-37 0.09735446837744632 1101 38-39 0.16707089672491549 1101 40-41 0.10794035206665598 1101 42-43 0.08787054471330435 1101 44-45 0.11663205614600969 1101 46-47 0.12851695934170237 1101 48-49 0.009173503423347995 1101 50-51 0.0542518941904504 1101 52-53 -0.02671186534703196 1101 54-55 0.12784556302536743 1101 56-57 0.12698634551887977 1101 58-59 0.18090906173604537 1101 60-61 0.11645498733074788 1101 62-63 0.23189077983860784 1101 64-65 0.19927884931311723 1101 66-67 0.21900375029853336 1101 68-69 0.1535288763520697 1101 70-71 0.33869276176302066 1101 72-73 0.3076911380705738 1101 74-75 0.49148293221142936 1101 76-77 0.4623935031638773 1101 78-79 0.5498312422523668 1101 80-81 0.5908863289997939 1101 82-83 0.8723964635502313 1101 84-85 1.1158219636843434 1101 86-87 1.1500073624060434 1101 88-89 1.270636176458158 1101 90-91 1.3233781143229386 1101 92-93 1.6422855835611898 1101 94-95 1.578385629625096 1101 96-97 1.707872097125584 1101 98-99 1.8358239750489211 1101 100-101 2.222986321635883 1102 1 0.10166287841735766 1102 2 0.14161029948503057 1102 3 0.21192265317537817 1102 4 0.11132618062679 1102 5 0.006843673209708356 1102 6 0.09709448367162565 1102 7 0.16923896588428278 1102 8 0.13998316017653423 1102 9 0.0698870694108038 1102 10-11 0.20039308669213796 1102 12-13 0.20144588772893712 1102 14-15 0.23531441840461298 1102 16-17 0.30960746740277756 1102 18-19 0.2911832033426265 1102 20-21 0.28591211117315396 1102 22-23 0.3501675859495421 1102 24-25 0.2588989740891847 1102 26-27 0.270841809559073 1102 28-29 0.17481547285783705 1102 30-31 0.3846037305253134 1102 32-33 0.23102884099967014 1102 34-35 0.2388062243179192 1102 36-37 0.4302518521560188 1102 38-39 0.46940755431008796 1102 40-41 0.377096000252088 1102 42-43 0.5131259781049593 1102 44-45 0.6805471815937949 1102 46-47 0.5988478588113963 1102 48-49 0.6391545309918953 1102 50-51 0.8199323205285864 1102 52-53 0.6907253452513231 1102 54-55 0.6967446641059709 1102 56-57 0.7405715498175525 1102 58-59 0.834704334176493 1102 60-61 0.9902590559215056 1102 62-63 0.9064530905619606 1102 64-65 0.9496171123922608 1102 66-67 0.8454988556383256 1102 68-69 0.9655749290468592 1102 70-71 0.9128405091361955 1102 72-73 0.8209500025723031 1102 74-75 0.872229146241768 1102 76-77 0.9607388747903087 1102 78-79 1.1135105225578137 1102 80-81 1.028435190435534 1102 82-83 1.141811541392233 1102 84-85 1.2547024329987089 1102 86-87 1.5045144941010982 1102 88-89 1.578898661619565 1102 90-91 1.7908275539286755 1102 92-93 2.010955434428446 1102 94-95 2.035906207113765 1102 96-97 2.0934286785589826 1102 98-99 2.3444410577493286 1102 100-101 2.8325420680027733 1103 1 0.3476947512668431 1103 2 0.32795887681823643 1103 3 0.4463686600144072 1103 4 0.27888767328175845 1103 5 0.22319288970432893 1103 6 0.28923918981715957 1103 7 0.2575705415237124 1103 8 0.23133051245035574 1103 9 0.35953691547943123 1103 10-11 0.38804741754339034 1103 12-13 0.34934251402190597 1103 14-15 0.4583266263877377 1103 16-17 0.4012227348385622 1103 18-19 0.39315741317932407 1103 20-21 0.4449317440814369 1103 22-23 0.4676401286401983 1103 24-25 0.5025707433945286 1103 26-27 0.5494589305629844 1103 28-29 0.5217991635889305 1103 30-31 0.5388917047031967 1103 32-33 0.5381911773477128 1103 34-35 0.5532834968030826 1103 36-37 0.5185077936940559 1103 38-39 0.6124087367587165 1103 40-41 0.6385905194429071 1103 42-43 0.6237283344800559 1103 44-45 0.6332377848556447 1103 46-47 0.5242529273614593 1103 48-49 0.6713120736292879 1103 50-51 0.7401833511763769 1103 52-53 0.754471576862116 1103 54-55 0.8146688808669182 1103 56-57 0.8476657089474386 1103 58-59 0.8966329504985495 1103 60-61 0.859564393984634 1103 62-63 0.885107762299306 1103 64-65 0.9653412822813578 1103 66-67 0.8865484652172313 1103 68-69 0.9649906289818801 1103 70-71 1.1604497791073243 1103 72-73 0.913310159379904 1103 74-75 0.901501674018661 1103 76-77 0.6345678778050186 1103 78-79 1.04367867527478 1103 80-81 1.2087817757156465 1103 82-83 1.3734830550330024 1103 84-85 1.3871031232930413 1103 86-87 1.418460729972967 1103 88-89 1.5072097840935434 1103 90-91 1.4715781424898182 1103 92-93 1.8135467013529052 1103 94-95 1.8220185610387496 1103 96-97 2.047816521708768 1103 98-99 2.5955694439732717 1103 100-101 2.9344017614183464 1104 1 0.14125474696992768 1104 2 0.13209954225442289 1104 3 0.20480917212878325 1104 4 0.07775289657495676 1104 5 0.09317160043392647 1104 6 0.1300618541009939 1104 7 0.18722463970826198 1104 8 0.11652812180200556 1104 9 0.14832000906432796 1104 10-11 0.11850596694328885 1104 12-13 0.10655938137413301 1104 14-15 0.193148499648224 1104 16-17 0.2629806621620716 1104 18-19 0.23995889561567196 1104 20-21 0.25737668983286 1104 22-23 0.22472515876744836 1104 24-25 0.24486217202176164 1104 26-27 0.1872788799765459 1104 28-29 0.24882335327828287 1104 30-31 0.23330839822558858 1104 32-33 0.3338776586610095 1104 34-35 0.31462613686793617 1104 36-37 0.2344151756020807 1104 38-39 0.24545593448524983 1104 40-41 0.2347503940510549 1104 42-43 0.3479324282700347 1104 44-45 0.32669401977980783 1104 46-47 0.33926918728587196 1104 48-49 0.41871024158015047 1104 50-51 0.3495943317523498 1104 52-53 0.4799581538794726 1104 54-55 0.5582093451292636 1104 56-57 0.6375489109318337 1104 58-59 0.7730987851258391 1104 60-61 0.7918449826622478 1104 62-63 0.6844026772887091 1104 64-65 0.6831048834884044 1104 66-67 0.7142191455949671 1104 68-69 0.9043752909414735 1104 70-71 0.761666832008224 1104 72-73 0.8607229759113153 1104 74-75 0.7907002990052341 1104 76-77 0.7485919405400061 1104 78-79 0.985135378953526 1104 80-81 1.1523356678434986 1104 82-83 1.1530331482956306 1104 84-85 1.1935777958972658 1104 86-87 1.1020526107530522 1104 88-89 1.301924087944169 1104 90-91 1.3347696775634041 1104 92-93 1.5498043796505456 1104 94-95 1.7667697304837624 1104 96-97 1.997960077798183 1104 98-99 1.9994720699754787 1104 100-101 2.4423498370831496 1105 1 0.021352542624335058 1105 2 0.02675125278214807 1105 3 0.12957104616310033 1105 4 0.08801032714470125 1105 5 -0.027243600911120325 1105 6 0.023401855319853837 1105 7 0.07538663347773422 1105 8 0.0051919994283835535 1105 9 -0.015728616311633914 1105 10-11 0.045886061512540266 1105 12-13 -0.024264353430446306 1105 14-15 0.006409705101546592 1105 16-17 0.04698150102509402 1105 18-19 0.0830126437918608 1105 20-21 0.08865355406252462 1105 22-23 0.13428497077608625 1105 24-25 0.032404876960661966 1105 26-27 0.13076621995625715 1105 28-29 0.15618007439935155 1105 30-31 0.13543979141932283 1105 32-33 -0.02961763976271925 1105 34-35 0.015065105998495198 1105 36-37 0.10301965168910954 1105 38-39 0.11280157831262017 1105 40-41 0.14464682673230556 1105 42-43 0.040893327932700174 1105 44-45 0.12397327154268112 1105 46-47 0.22171400694824683 1105 48-49 0.2400193339231933 1105 50-51 0.33080971520954705 1105 52-53 0.26654781722294985 1105 54-55 0.30887810536858495 1105 56-57 0.2641286799935685 1105 58-59 0.266009496402809 1105 60-61 0.3553668092326987 1105 62-63 0.30982040597746874 1105 64-65 0.4085502157892904 1105 66-67 0.49732250857529436 1105 68-69 0.40209960613887574 1105 70-71 0.5457800570063469 1105 72-73 0.6389139713979404 1105 74-75 0.6129231784563167 1105 76-77 0.7578983012009175 1105 78-79 0.9379715604777523 1105 80-81 1.0520343582851197 1105 82-83 1.0212787774249712 1105 84-85 1.0136490275560988 1105 86-87 1.032315669591764 1105 88-89 1.1307293534228045 1105 90-91 1.221730914205228 1105 92-93 1.4562354835440914 1105 94-95 1.5815231441902107 1105 96-97 1.7593689733310605 1105 98-99 2.0809215464030686 1105 100-101 2.2222418603562204 1106 1 0.014215304549523466 1106 2 0.026960435360997792 1106 3 0.10295837363216975 1106 4 0.024465308193114765 1106 5 0.029763121537570214 1106 6 -0.04500978738914796 1106 7 -0.037042745053341264 1106 8 -0.051618055638485316 1106 9 -0.13528092983519713 1106 10-11 0.05585620382820622 1106 12-13 -0.078803794179926 1106 14-15 -0.02198459512015205 1106 16-17 -0.04873830159237258 1106 18-19 0.02449873404430747 1106 20-21 -0.04119966055522184 1106 22-23 -0.016431077282270223 1106 24-25 -2.687267009306993E-4 1106 26-27 0.0024639958269574436 1106 28-29 -0.02830555100292287 1106 30-31 0.03556762521751011 1106 32-33 0.01963234235842748 1106 34-35 -0.26092359456648495 1106 36-37 -0.03776290570201013 1106 38-39 -0.05884345494204979 1106 40-41 -0.10527271842968133 1106 42-43 -0.10755210580827423 1106 44-45 0.03438233969686877 1106 46-47 -0.10833766293661284 1106 48-49 -0.006342319513116479 1106 50-51 0.05811876906064839 1106 52-53 -0.03886321760506206 1106 54-55 0.014366913206679044 1106 56-57 0.09925347438675658 1106 58-59 0.20085806251880456 1106 60-61 0.35451130612178616 1106 62-63 0.37578085795487226 1106 64-65 0.26480066276061365 1106 66-67 0.19530309214105301 1106 68-69 -0.20643933400273085 1106 70-71 0.1698789628205546 1106 72-73 0.27242519931755993 1106 74-75 0.4505787244764825 1106 76-77 0.3249779693694066 1106 78-79 0.5553506994321928 1106 80-81 0.7974287657799373 1106 82-83 0.7255822173162656 1106 84-85 0.9105908497687842 1106 86-87 1.0425012520847936 1106 88-89 0.9345938674755807 1106 90-91 0.7253308212351932 1106 92-93 0.6082379150680843 1106 94-95 1.0405725255819007 1106 96-97 1.2859959489443007 1106 98-99 1.412586782761501 1106 100-101 1.8502964623729525 1107 1 0.03945315719791864 1107 2 0.05642559390648216 1107 3 0.06686363186235411 1107 4 0.06936266324412799 1107 5 0.05800012053246206 1107 6 0.034187227577319845 1107 7 -0.0020030226284148966 1107 8 -0.008918514133021915 1107 9 0.016976432926234963 1107 10-11 -0.009382715887049642 1107 12-13 0.027872258085700707 1107 14-15 0.009820365708172574 1107 16-17 0.010616088527278578 1107 18-19 0.02519542544857245 1107 20-21 0.01752154584993093 1107 22-23 -0.012609084689778172 1107 24-25 0.10710401696895389 1107 26-27 0.10789777572204429 1107 28-29 0.021188969092349907 1107 30-31 0.13256589725760648 1107 32-33 0.07650924414696902 1107 34-35 0.10124165622865888 1107 36-37 0.2510175745469212 1107 38-39 0.26002211528471975 1107 40-41 0.30636622744592756 1107 42-43 0.19988896456306549 1107 44-45 0.24245349584661824 1107 46-47 0.37142231585973917 1107 48-49 0.35322169761501954 1107 50-51 0.15142946451287997 1107 52-53 0.13124496920737272 1107 54-55 0.20974203884519937 1107 56-57 0.15103788557237152 1107 58-59 0.2477998832791144 1107 60-61 0.2136830901199076 1107 62-63 0.2762975248510102 1107 64-65 0.10874936093902932 1107 66-67 0.24851892190602598 1107 68-69 0.27707826915854383 1107 70-71 0.3625406406627363 1107 72-73 0.16905378431804152 1107 74-75 0.17966679535726016 1107 76-77 0.2820519343145378 1107 78-79 0.4551787639794149 1107 80-81 0.6210825404429485 1107 82-83 0.6980715122260115 1107 84-85 0.7974423484814217 1107 86-87 0.8986644238206019 1107 88-89 0.9892970507770187 1107 90-91 0.9814136187403193 1107 92-93 1.2804122531424085 1107 94-95 1.4753979740942036 1107 96-97 1.4422183256808658 1107 98-99 1.554554522920391 1107 100-101 2.096116141839836 1108 1 0.0027104252480398827 1108 2 0.03940436619475918 1108 3 0.13925267255358875 1108 4 0.09817015325388923 1108 5 -0.005476563447878391 1108 6 -0.012864606769390718 1108 7 -0.05754265945155623 1108 8 -0.0017764189324438462 1108 9 0.033970910603080995 1108 10-11 0.02576075784293863 1108 12-13 0.07906386546739697 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-2.220690471752384 2316 92-93 -2.7129534431589732 2316 94-95 -2.8151560093163077 2316 96-97 -2.9920053951857817 2316 98-99 -3.518863402466181 2316 100-101 -4.2619904438329925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 24.0 6 67.0 7 194.0 8 426.0 9 919.0 10 1563.0 11 2792.0 12 4133.0 13 6044.0 14 8420.0 15 11301.0 16 14507.0 17 20203.0 18 20370.0 19 15618.0 20 14265.0 21 14484.0 22 15509.0 23 16924.0 24 19584.0 25 22345.0 26 26289.0 27 31939.0 28 38607.0 29 46519.0 30 56675.0 31 69736.0 32 87886.0 33 113778.0 34 157211.0 35 230560.0 36 354992.0 37 499159.0 38 333016.0 39 23643.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8752277689999 18.855193747307787 15.169832111108283 23.099746372584026 2 15.607712386759964 19.65241615301937 40.51880936190748 24.22106209831319 3 19.71642293884169 23.531096792759406 29.578494748183008 27.173985520215897 4 13.117131438452336 16.711299321276595 36.57855500738906 33.593014232882005 5 13.875054283220233 37.013830706537235 34.29392334989977 14.817191660342763 6 32.73445782963412 35.96906984507287 16.92600441195113 14.370467913341875 7 28.88983832239194 32.41798780728528 20.081176564510937 18.61099730581185 8 26.270068253996104 35.4780850279859 19.069227799905253 19.18261891811275 9 25.876488449030187 16.99724791005219 20.03187230723746 37.09439133368016 10-11 25.175503855294636 26.57411737045131 26.75247245058827 21.49790632366578 12-13 25.996196027410363 23.641764215110438 27.43490215034991 22.927137607129293 14-15 22.659232134444462 25.210880975838286 25.832338328971687 26.297548560745565 16-17 22.21174336169059 28.454390491472203 26.632172694740852 22.701693452096354 18-19 22.11199435720671 26.955940241836508 28.99871124637127 21.933354154585516 20-21 22.686231040451425 26.002644182053775 29.491424831624208 21.819699945870592 22-23 22.43388062971727 25.884455623197834 29.335140279196388 22.346523467888513 24-25 22.309790440162075 26.06950176844763 29.37761522106363 22.243092570326663 26-27 22.00959320468538 26.445808704083746 29.211823794789222 22.332774296441652 28-29 22.090524473453918 26.64256277648741 29.129913168970685 22.136999581087988 30-31 22.206810969507433 26.273627084384728 29.104984187747657 22.414577758360185 32-33 22.006300574193176 26.218973356901447 29.105204849814676 22.6695212190907 34-35 22.51176329938337 26.363895609903764 28.955682249428012 22.16865884128485 36-37 22.52041978847385 26.1495028139585 28.9228827534109 22.407194644156753 38-39 22.12685340147941 26.48228792155567 28.857660389742723 22.533198287222195 40-41 22.66431845319774 26.18731872588359 28.815742682838273 22.33262013808039 42-43 22.339236038978346 26.210702526756975 28.787322056162388 22.662739378102287 44-45 22.351597998238642 26.551414404741408 28.73141719044919 22.36557040657076 46-47 22.557403196260502 26.76126832726226 28.1654884228553 22.51584005362194 48-49 22.46356071029007 26.672987628066913 28.261913268601603 22.601538393041416 50-51 22.610892907117623 26.89876221749845 28.080002737175846 22.41034213820808 52-53 22.75242332179099 26.7861827012241 27.958036111420604 22.50335786556431 54-55 22.58898234082928 26.884571890783178 27.97994667770895 22.5464990906786 56-57 22.511143902633744 27.06161102099291 27.87078863124539 22.55645644512796 58-59 22.499717070765644 26.793376560826275 27.904849801861808 22.802056566546273 60-61 22.415123422943676 26.952496839526923 27.978762322774443 22.653617414754954 62-63 22.367464103079236 26.937670470346823 28.115072802736474 22.57979262383747 64-65 22.20433017710054 26.958791466678832 27.991768294555214 22.845110061665412 66-67 22.215861773858865 26.977096025443547 27.864488235034795 22.94255396566279 68-69 22.450674217901312 26.76226485278536 28.01474569962787 22.772315229685457 70-71 22.46703764757282 26.77523838432932 27.673045222180598 23.084678745917266 72-73 22.409793825738404 26.91540898407788 27.5917414491767 23.083055741007016 74-75 22.336407684796654 27.1957721160738 27.467537708545166 23.00028249058438 76-77 22.447693183642567 26.841167475683434 27.681050584237905 23.030088756436093 78-79 22.468529057490343 26.98436476026463 27.70506667040981 22.842039511835218 80-81 22.47838218515719 26.95602129562573 27.519512290341304 23.046084228875777 82-83 22.512502217949724 26.994635906418203 27.5980498272686 22.894812048363477 84-85 22.543428290680026 27.014721751870923 27.501030873566645 22.940819083882403 86-87 22.60727002755842 26.973322176906855 27.401689474832793 23.01771832070193 88-89 22.587917556578198 27.110729777898264 27.195762112995407 23.105590552528138 90-91 22.478538689797954 27.05591335067975 27.252129790616923 23.213418168905374 92-93 22.517169969649608 26.76278135742883 27.437341728564242 23.282706944357322 94-95 22.400114399988947 26.762228088925816 27.402657474896593 23.435000036188647 96-97 22.53225346221778 26.616774498057104 27.425142556102923 23.425829483622195 98-99 22.424518109468757 26.61391601333322 27.219122181286377 23.742443695911643 100-101 22.67956602090936 26.38645247579781 27.307712722441043 23.626268780851788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 664.0 1 450.0 2 328.0 3 446.5 4 367.0 5 323.5 6 680.0 7 1626.5 8 2706.5 9 3189.5 10 2727.5 11 2080.5 12 2010.0 13 2180.0 14 2405.0 15 2568.5 16 2689.5 17 2909.0 18 3168.5 19 3440.0 20 3846.0 21 4542.0 22 5467.5 23 6699.5 24 8434.0 25 10775.0 26 13763.5 27 16932.5 28 19481.0 29 22387.5 30 25852.5 31 29777.0 32 34893.0 33 38429.0 34 40663.5 35 43997.0 36 48541.0 37 53017.5 38 56670.0 39 59617.5 40 62874.5 41 67255.0 42 69875.0 43 72496.0 44 76775.5 45 79949.0 46 81452.0 47 84855.0 48 87256.0 49 86777.5 50 86052.0 51 84301.5 52 82698.5 53 81774.5 54 81173.0 55 77224.0 56 71079.0 57 64978.0 58 59624.0 59 52864.5 60 42893.0 61 34930.0 62 28595.0 63 21365.0 64 15400.5 65 11737.0 66 8854.0 67 6704.0 68 5170.5 69 3840.0 70 2719.5 71 1730.5 72 1184.5 73 965.5 74 687.0 75 444.0 76 305.0 77 185.0 78 109.0 79 63.5 80 41.0 81 23.0 82 10.0 83 7.0 84 3.5 85 0.5 86 2.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011931279340200253 3 0.005702449684654533 4 4.386499757426564E-5 5 5.263799708911876E-4 6 2.1932498787132816E-4 7 0.0 8 8.772999514853127E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.193249878713282E-5 24-25 0.0017107349053963597 26-27 0.022129891276217013 28-29 3.0705498301985943E-4 30-31 1.0966249393566408E-4 32-33 0.003882052285322509 34-35 0.0169757540612408 36-37 0.008465944531833266 38-39 0.02195443128591995 40-41 0.023796761184039106 42-43 0.020024371392652264 44-45 0.010067016943293962 46-47 0.0025441698593074067 48-49 2.41257486658461E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.728524793812579E-4 68-69 2.8512248423272663E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1932498787132816E-4 82-83 0.0011624224357180393 84-85 0.004474229752575095 86-87 8.553674526981798E-4 88-89 4.386499757426564E-5 90-91 0.004232972265916633 92-93 6.579749636139845E-4 94-95 6.579749636139846E-5 96-97 2.41257486658461E-4 98-99 1.315949927227969E-4 100-101 0.0032021448229213913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2279722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.97662547728896 #Duplication Level Percentage of deduplicated Percentage of total 1 74.20302644099898 40.79431993928172 2 14.240597793168575 15.658000228954732 3 5.004294113731654 8.253576098064807 4 2.254171851741472 4.957070466185514 5 1.231086588183653 3.3840493144343093 6 0.7191698865109966 2.3722520103155684 7 0.45587201162752866 1.7543613394177475 8 0.35175973266334437 1.547085046449918 9 0.23449876173803294 1.1602755539063874 >10 1.199765798213017 11.61070019704407 >50 0.06914929979260741 2.588186059744942 >100 0.03301459228079218 3.396376641567737 >500 0.0023954195663063262 0.8653047889535846 >1k 0.0010380151453992781 0.9238199717698012 >5k 1.596946377537351E-4 0.7346223439091293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9060 0.39741687802284664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7675 0.33666385638248875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4778 0.2095869584098412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.315949927227969E-4 2 0.0 0.0 0.0 0.0 1.315949927227969E-4 3 0.0 0.0 0.0 0.0 1.315949927227969E-4 4 0.0 0.0 0.0 0.0 1.315949927227969E-4 5 0.0 0.0 0.0 0.0 1.7545999029706255E-4 6 0.0 0.0 0.0 0.0 2.1932498787132816E-4 7 0.0 0.0 0.0 0.0 2.1932498787132816E-4 8 0.0 0.0 0.0 0.0 2.1932498787132816E-4 9 0.0 0.0 0.0 0.0 2.1932498787132816E-4 10-11 0.0 0.0 0.0 0.0 2.1932498787132816E-4 12-13 0.0 0.0 0.0 0.0 7.018399611882502E-4 14-15 0.0 0.0 0.0 0.0 7.23772459975383E-4 16-17 0.0 0.0 0.0 4.386499757426564E-5 7.895699563367814E-4 18-19 4.386499757426564E-5 0.0 0.0 6.579749636139846E-5 8.553674526981798E-4 20-21 4.386499757426564E-5 0.0 0.0 1.5352749150992974E-4 9.211649490595784E-4 22-23 4.386499757426564E-5 0.0 0.0 2.41257486658461E-4 9.211649490595784E-4 24-25 8.772999514853127E-5 0.0 0.0 4.386499757426563E-4 9.211649490595784E-4 26-27 8.772999514853127E-5 0.0 0.0 9.211649490595784E-4 9.430974478467112E-4 28-29 8.772999514853127E-5 0.0 0.0 0.0031802123241342585 9.65029946633844E-4 30-31 1.315949927227969E-4 0.0 0.0 0.010461801921462354 0.0010088949442081097 32-33 1.315949927227969E-4 0.0 0.0 0.025880348568816724 0.0010527599417823753 34-35 1.9739248908419535E-4 0.0 0.0 0.0495893797577073 0.0010527599417823753 36-37 2.631899854455938E-4 0.0 0.0 0.08950652755028903 0.0010527599417823753 38-39 3.947849781683907E-4 0.0 0.0 0.14973316921975574 0.0011404899369309065 40-41 3.947849781683907E-4 0.0 0.0 0.21329355070486666 0.0011843549345051721 42-43 4.605824745297891E-4 0.0 0.0 0.27402463984643743 0.0012501524308665705 44-45 4.82514973316922E-4 0.0 0.0 0.3505471281147438 0.001315949927227969 46-47 5.483124696783205E-4 0.0 0.0 0.44027298065290416 0.0013817474235893676 48-49 5.702449684654533E-4 0.0 0.0 0.5338150879800256 0.0014256124211636332 50-51 5.702449684654533E-4 0.0 0.0 0.6329719149966531 0.0014914099175250316 52-53 5.702449684654533E-4 0.0 0.0 0.7253077348904823 0.0017545999029706253 54-55 5.702449684654533E-4 0.0 0.0 0.825999836822209 0.0018642623969062895 56-57 5.702449684654533E-4 0.0 0.0 0.9461241326793355 0.0019081273944805551 58-59 5.702449684654533E-4 0.0 0.0 1.0738809381143841 0.001930059893267688 60-61 5.921774672525861E-4 0.0 0.0 1.206616420774112 0.0019739248908419533 62-63 6.141099660397189E-4 0.0 0.0 1.336588408586661 0.0019739248908419533 64-65 6.141099660397189E-4 0.0 0.0 1.4755307884031472 0.0020177898884162194 66-67 6.141099660397189E-4 0.0 0.0 1.6302645673463694 0.002039722387203352 68-69 6.141099660397189E-4 0.0 0.0 1.8007020154211786 0.0021055198835647506 70-71 6.579749636139846E-4 0.0 0.0 1.9764690607012612 0.002171317379926149 72-73 7.018399611882502E-4 0.0 0.0 2.1541003683782494 0.002280979873861813 74-75 7.018399611882502E-4 0.0 0.0 2.3371928682532346 0.0024564398641588755 76-77 7.018399611882502E-4 0.0 0.0 2.5350240073131722 0.0025441698593074067 78-79 7.018399611882502E-4 0.0 0.0 2.752309272797297 0.0025880348568816723 80-81 7.018399611882502E-4 0.0 0.0 2.9817451426094936 0.002631899854455938 82-83 7.457049587625158E-4 0.0 0.0 3.220392661912286 0.002741562348391602 84-85 7.676374575496486E-4 0.0 0.0 3.4580970837672314 0.0027634948471787347 86-87 7.895699563367814E-4 0.0 0.0 3.7096409123568574 0.002917022338688665 88-89 8.115024551239142E-4 0.0 0.0 3.977546385041685 0.0030705498301985944 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4735 0.0 51.569298 1 GTATCAA 7890 0.0 39.37756 1 ATCAACG 9795 0.0 31.279203 3 TCAACGC 9945 0.0 30.948675 4 TATCAAC 10150 0.0 30.469337 2 CAACGCA 10120 0.0 30.413494 5 AACGCAG 10430 0.0 29.55444 6 GTGGTAT 2565 0.0 25.929176 1 ACGCAGA 12030 0.0 25.54471 7 TGGTATC 2390 0.0 25.24375 2 CGCAGAG 12065 0.0 25.234404 8 TACATGG 7215 0.0 23.506067 2 GTACATG 7440 0.0 22.922901 1 GAGTACT 8645 0.0 22.415968 12-13 GCAGAGT 13570 0.0 22.260738 9 ACATGGG 7450 0.0 21.86947 3 GTGTAAG 1050 0.0 21.26457 1 CAGAGTA 12585 0.0 21.172491 10-11 GTACTTT 9140 0.0 21.046078 14-15 GTAAGGT 1010 0.0 20.692034 4 >>END_MODULE