FastQCFastQC Report
Thu 26 May 2016
SRR938233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93670.4076601993169831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77350.3366341028842601No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.20106652298969382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44000.053.311581
GTATCAA76600.040.601771
ATCAACG94550.032.5687263
TCAACGC94950.032.4815674
CAACGCA96650.031.8119075
TATCAAC97000.031.7461152
AACGCAG101550.030.3705086
ACGCAGA115850.026.5090487
CGCAGAG116650.026.1630888
TGGTATC21100.023.6479852
GCAGAGT130000.023.365119
GAGTACT79800.022.93163512-13
GTGGTAT22600.022.830111
CAGAGTA122900.021.31083910-11
TACATGG70700.021.1728062
GTACTTT86050.021.08610714-15
GTACATG73550.021.0452961
AGAGTAC112300.020.82505810-11
AGTACTT84350.019.77876912-13
GTACCGT1707.931042E-519.5675496
Produced by FastQC (version 0.11.5)