##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938233_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911032850875227 33.0 31.0 34.0 30.0 34.0 2 32.09882637209405 34.0 31.0 34.0 30.0 34.0 3 32.17293287729241 34.0 31.0 34.0 30.0 34.0 4 35.684082712326465 37.0 35.0 37.0 35.0 37.0 5 35.70110547418841 37.0 35.0 37.0 35.0 37.0 6 35.72817198760351 37.0 36.0 37.0 35.0 37.0 7 35.66936046483795 37.0 35.0 37.0 33.0 37.0 8 35.6576529095675 37.0 35.0 37.0 33.0 37.0 9 37.32620736747779 39.0 37.0 39.0 34.0 39.0 10-11 37.245208240942105 39.0 37.0 39.0 34.0 39.0 12-13 37.150522881762 39.0 37.0 39.0 34.0 39.0 14-15 38.40608343738454 40.0 38.0 41.0 34.0 41.0 16-17 38.328611679179645 40.0 38.0 41.0 33.5 41.0 18-19 38.26934906236413 40.0 38.0 41.0 33.5 41.0 20-21 38.1668171038848 40.0 38.0 41.0 33.5 41.0 22-23 38.01520228293193 40.0 38.0 41.0 33.0 41.0 24-25 37.97192162583609 40.0 38.0 41.0 33.0 41.0 26-27 37.98462602714746 40.0 38.0 41.0 33.0 41.0 28-29 37.90182839973244 40.0 38.0 41.0 33.0 41.0 30-31 37.720173717994186 40.0 37.5 41.0 33.0 41.0 32-33 37.49768882300793 40.0 37.0 41.0 32.0 41.0 34-35 37.2966603807991 40.0 37.0 41.0 31.5 41.0 36-37 37.08218594127204 40.0 36.5 41.0 31.0 41.0 38-39 36.843517149625264 40.0 36.0 41.0 30.0 41.0 40-41 36.62601213275439 39.5 36.0 41.0 30.0 41.0 42-43 36.338117512502464 39.0 35.5 41.0 30.0 41.0 44-45 36.11558452693008 39.0 35.0 41.0 29.0 41.0 46-47 35.98580609614548 39.0 35.0 41.0 28.5 41.0 48-49 35.74488401029356 39.0 35.0 41.0 27.5 41.0 50-51 35.10616943466796 38.5 34.5 40.5 26.5 40.5 52-53 35.18273204143015 38.5 35.0 40.0 27.0 41.0 54-55 35.32530844344482 39.0 35.0 40.5 26.5 41.0 56-57 35.119996892608285 39.0 35.0 40.5 26.0 41.0 58-59 34.86085043305464 38.0 34.5 40.0 26.0 41.0 60-61 34.49235098555237 38.0 34.0 40.0 25.0 41.0 62-63 34.0771414346314 37.5 34.0 40.0 23.5 41.0 64-65 33.650150778131795 37.0 33.5 40.0 22.0 41.0 66-67 33.22152830577083 36.0 33.0 39.0 21.5 41.0 68-69 32.733927408021856 36.0 32.5 39.0 19.5 40.5 70-71 32.26015026893735 35.0 32.0 38.5 18.0 40.0 72-73 31.77282115916156 35.0 31.5 37.5 14.5 40.0 74-75 31.246442711055657 35.0 31.0 37.0 9.5 39.0 76-77 30.713590312597514 35.0 30.5 36.5 7.0 39.0 78-79 30.151677926246883 34.0 30.0 36.0 3.5 38.5 80-81 29.631303185250594 34.0 29.5 35.5 2.0 37.0 82-83 29.09687032558415 34.0 29.0 35.0 2.0 37.0 84-85 28.603614975887247 34.0 29.0 35.0 2.0 36.5 86-87 28.10796902356961 34.0 27.5 35.0 2.0 36.0 88-89 27.559050670069418 33.5 26.5 35.0 2.0 35.5 90-91 26.96184893288948 33.0 25.5 35.0 2.0 35.0 92-93 26.351027332426067 32.5 24.5 35.0 2.0 35.0 94-95 25.74995158300718 32.0 23.5 35.0 2.0 35.0 96-97 24.925530095349924 32.0 18.5 34.0 2.0 35.0 98-99 23.869465393709575 31.5 2.0 34.0 2.0 35.0 100-101 20.986484804462805 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13694655166949588 1101 2 0.07634490267493277 1101 3 0.0038264799775831193 1101 4 0.035629320064643366 1101 5 0.02810282046890933 1101 6 -0.02955502351480277 1101 7 0.05778929122584486 1101 8 0.04822669695226267 1101 9 -0.011493393726290435 1101 10-11 5.718903753333393E-4 1101 12-13 0.05438209953720019 1101 14-15 0.009955041680250076 1101 16-17 0.1665969722138172 1101 18-19 0.06889610733219342 1101 20-21 0.11758491358225598 1101 22-23 0.1652599747769372 1101 24-25 0.1591888024494068 1101 26-27 0.04779471491268339 1101 28-29 0.21795961357089055 1101 30-31 0.15969206045466677 1101 32-33 0.2254402299505216 1101 34-35 0.09871236714876375 1101 36-37 0.06455140998228615 1101 38-39 0.14004938151521884 1101 40-41 0.2019970732333789 1101 42-43 0.08511183059121663 1101 44-45 0.2631303037948882 1101 46-47 0.13803036906318766 1101 48-49 0.4402089684536179 1101 50-51 0.3316396965705479 1101 52-53 0.36210962970316984 1101 54-55 0.27717467215283165 1101 56-57 0.3808305545503501 1101 58-59 0.32763038994315963 1101 60-61 0.20338298742527883 1101 62-63 0.26069018459235593 1101 64-65 0.36256132116690765 1101 66-67 0.13036981686087756 1101 68-69 0.19683670239834328 1101 70-71 0.20606147582171985 1101 72-73 0.20687259766226873 1101 74-75 0.33022798188288505 1101 76-77 0.30675005309500136 1101 78-79 0.3911298842432416 1101 80-81 0.47411735567412805 1101 82-83 0.3189417841791169 1101 84-85 0.5264776393580668 1101 86-87 0.5063753748597826 1101 88-89 0.5844770397036463 1101 90-91 0.6458850216743031 1101 92-93 0.5820893729090351 1101 94-95 0.8114730652714357 1101 96-97 0.871800088223722 1101 98-99 0.9802773849082804 1101 100-101 1.0910965244385977 1102 1 0.31605705738998324 1102 2 0.3572101075012952 1102 3 0.2386405232161053 1102 4 0.1828369558036229 1102 5 0.21758161779138163 1102 6 0.30972364530330054 1102 7 0.22216782886874142 1102 8 0.23129026448795287 1102 9 0.33938953636433666 1102 10-11 0.3422185963055355 1102 12-13 0.20816022126718536 1102 14-15 0.3105703556745212 1102 16-17 0.3464058051409893 1102 18-19 0.18444298591163744 1102 20-21 0.25562070762870803 1102 22-23 0.23221470141636757 1102 24-25 0.28986621241112687 1102 26-27 0.3367508308237106 1102 28-29 0.3061030121096664 1102 30-31 0.2673466038917667 1102 32-33 0.31710316721116527 1102 34-35 0.4556685290937921 1102 36-37 0.433778023457954 1102 38-39 0.3526423533817109 1102 40-41 0.49178809838249293 1102 42-43 0.6218225400773321 1102 44-45 0.45788412065467554 1102 46-47 0.39040953467942785 1102 48-49 0.36521609612481143 1102 50-51 0.47028739548903076 1102 52-53 0.4488669051934693 1102 54-55 0.44247302137767264 1102 56-57 0.45710358485397506 1102 58-59 0.3755959799569055 1102 60-61 0.21971691724643705 1102 62-63 0.23894389320707887 1102 64-65 0.09246535980420134 1102 66-67 0.059070413630983865 1102 68-69 0.12275680169283021 1102 70-71 0.21015654820694607 1102 72-73 0.23977627302176074 1102 74-75 0.2271290680466045 1102 76-77 0.15183313253558595 1102 78-79 0.10386638667055692 1102 80-81 0.2886323342064294 1102 82-83 0.3709475097673618 1102 84-85 0.5401208597321947 1102 86-87 0.24627715305360098 1102 88-89 0.3636879516742475 1102 90-91 0.6714081645115293 1102 92-93 0.6444891213722705 1102 94-95 0.6512880737041229 1102 96-97 0.7588257940911696 1102 98-99 0.9261239123047069 1102 100-101 0.84082743177974 1103 1 0.2839783230961359 1103 2 0.37551833412849334 1103 3 0.3227344204037763 1103 4 0.29139448245123134 1103 5 0.2211912195550525 1103 6 0.26286129989308904 1103 7 0.29253581513631133 1103 8 0.3618516575893409 1103 9 0.3584474932022559 1103 10-11 0.32656114139137316 1103 12-13 0.30196558591921274 1103 14-15 0.4901823850158209 1103 16-17 0.3457393782870142 1103 18-19 0.44655910153500855 1103 20-21 0.46836023107460534 1103 22-23 0.4762714486727191 1103 24-25 0.5719720834045177 1103 26-27 0.47487968176329076 1103 28-29 0.4626725125629889 1103 30-31 0.5498947610153593 1103 32-33 0.5833145053028872 1103 34-35 0.568670584330647 1103 36-37 0.590399588509257 1103 38-39 0.48101482456737443 1103 40-41 0.4993949990979374 1103 42-43 0.40511485281832194 1103 44-45 0.4992464460804982 1103 46-47 0.4962821057870457 1103 48-49 0.5001923567717625 1103 50-51 0.5138095076919171 1103 52-53 0.5577624835943453 1103 54-55 0.8233473279718879 1103 56-57 0.6529964769319534 1103 58-59 0.6703681684160259 1103 60-61 0.6138701154852768 1103 62-63 0.47989548680449445 1103 64-65 0.5751010275247808 1103 66-67 0.6160401278121128 1103 68-69 0.6627025493299357 1103 70-71 0.9199432200905875 1103 72-73 0.9720097285754292 1103 74-75 0.7077240138889067 1103 76-77 0.9259756443842235 1103 78-79 0.9353669409744576 1103 80-81 0.8667869755286794 1103 82-83 0.8662330867167327 1103 84-85 0.9776501789376155 1103 86-87 0.9221437919524824 1103 88-89 0.8904817611409577 1103 90-91 0.845641552749349 1103 92-93 0.9368544528712484 1103 94-95 0.8185773067452686 1103 96-97 0.9633371126192252 1103 98-99 1.1574310387385829 1103 100-101 1.0573097161428997 1104 1 0.26770684563590663 1104 2 0.2346628193979967 1104 3 0.19875015708608146 1104 4 0.10503963523015614 1104 5 0.1453054404593246 1104 6 0.20359473325437705 1104 7 0.20986861288686498 1104 8 0.11936891635064484 1104 9 0.04549623517343804 1104 10-11 0.19504465449936248 1104 12-13 0.08183850315199237 1104 14-15 0.23767026548633652 1104 16-17 0.27184943267229755 1104 18-19 0.27307320587126327 1104 20-21 0.24511554456774576 1104 22-23 0.23854456438616722 1104 24-25 0.39757766606739153 1104 26-27 0.3560411481574448 1104 28-29 0.4416168237680935 1104 30-31 0.3836778263603975 1104 32-33 0.34026684237348803 1104 34-35 0.43651021666882883 1104 36-37 0.39781563787738605 1104 38-39 0.33920668134623355 1104 40-41 0.31058966374829566 1104 42-43 0.4161807413147116 1104 44-45 0.5361384513260603 1104 46-47 0.3753546024298089 1104 48-49 0.5547599721288066 1104 50-51 0.48726626135140805 1104 52-53 0.38043161551703975 1104 54-55 0.37436395072864315 1104 56-57 0.34259888547921946 1104 58-59 0.4551245373222983 1104 60-61 0.42046664443628856 1104 62-63 0.37348936540836064 1104 64-65 0.3995588484117292 1104 66-67 0.34482553633182533 1104 68-69 0.5642953173871561 1104 70-71 0.5424380907040316 1104 72-73 0.6478996426852746 1104 74-75 0.5084819029051246 1104 76-77 0.49630494903237476 1104 78-79 0.4922224005714355 1104 80-81 0.5849924581635904 1104 82-83 0.5759999900637318 1104 84-85 0.43402381093711284 1104 86-87 0.46129312109567167 1104 88-89 0.4725962498366201 1104 90-91 0.4702472496173691 1104 92-93 0.5629349714414005 1104 94-95 0.6351852955925423 1104 96-97 0.49339669620361093 1104 98-99 0.5503162546847733 1104 100-101 0.5289283575741699 1105 1 0.36642723504199637 1105 2 0.36787805878103796 1105 3 0.3204191737704605 1105 4 0.3055283944356262 1105 5 0.2096088195920558 1105 6 0.23949106856113644 1105 7 0.23895175013827696 1105 8 0.3286072289044881 1105 9 0.4066105587167641 1105 10-11 0.36420423033970195 1105 12-13 0.2583763562291779 1105 14-15 0.3412613816780379 1105 16-17 0.408809132463098 1105 18-19 0.44236529454032336 1105 20-21 0.5000978415951636 1105 22-23 0.4904329185286471 1105 24-25 0.5272693615347492 1105 26-27 0.42112236832815597 1105 28-29 0.546940244029841 1105 30-31 0.6396937234518631 1105 32-33 0.5896678473211239 1105 34-35 0.5588158509289727 1105 36-37 0.5838756960273557 1105 38-39 0.6522303386854844 1105 40-41 0.6946496492478715 1105 42-43 0.6311595466754198 1105 44-45 0.6887904500272484 1105 46-47 0.6336474961837979 1105 48-49 0.7305368494371294 1105 50-51 0.622008456253127 1105 52-53 0.7955331481437398 1105 54-55 0.8704986534476902 1105 56-57 1.063096340491576 1105 58-59 0.9457528223431169 1105 60-61 0.9408317348860251 1105 62-63 0.9490765362390832 1105 64-65 0.9637701172285489 1105 66-67 0.9874797664378434 1105 68-69 0.9136981015280199 1105 70-71 0.9443646975008448 1105 72-73 0.8974003565865587 1105 74-75 0.8163006787312241 1105 76-77 0.8386775106109923 1105 78-79 0.9174466159842893 1105 80-81 0.9167917933383904 1105 82-83 0.9260718249901849 1105 84-85 1.014243370794361 1105 86-87 1.026198460602803 1105 88-89 1.0205125180381351 1105 90-91 1.079183914786551 1105 92-93 1.1913706676916007 1105 94-95 1.2431600844245452 1105 96-97 1.1328266945875107 1105 98-99 1.1796081876744289 1105 100-101 1.309364608339589 1106 1 0.08154972241064229 1106 2 0.12273056330184318 1106 3 0.1633092471945119 1106 4 0.08393711337734544 1106 5 0.09839946868272875 1106 6 0.17968800561314424 1106 7 0.22295688851212248 1106 8 0.1215279987294906 1106 9 0.07546585937419081 1106 10-11 -0.30306624132286686 1106 12-13 -0.09108652949437612 1106 14-15 -0.03657221618747286 1106 16-17 0.13728564535194465 1106 18-19 0.16666760608115538 1106 20-21 0.009340404264555957 1106 22-23 0.015044048956347922 1106 24-25 0.039248959339147405 1106 26-27 0.040470448057327246 1106 28-29 -0.051558469194489476 1106 30-31 0.0727599302059474 1106 32-33 0.24363248390337588 1106 34-35 -0.03265596875659327 1106 36-37 -0.03324980424397239 1106 38-39 0.0790879801253368 1106 40-41 0.2875837305453217 1106 42-43 0.03231131637385687 1106 44-45 0.1826011608889715 1106 46-47 0.35117659593547046 1106 48-49 0.2604803884266431 1106 50-51 0.13464871771081732 1106 52-53 0.15489762353351466 1106 54-55 -0.1673212037776679 1106 56-57 -0.13914293286205037 1106 58-59 0.03823973272854886 1106 60-61 -0.17259946807828896 1106 62-63 -0.03410190877725938 1106 64-65 -0.08452488682308967 1106 66-67 -0.19443241566891345 1106 68-69 -0.41712654055340437 1106 70-71 -0.3113805121875757 1106 72-73 -0.07344606393667874 1106 74-75 0.0616789427697384 1106 76-77 0.0105935406789186 1106 78-79 0.2958412233103971 1106 80-81 0.2515667785357394 1106 82-83 0.34491221388885407 1106 84-85 0.2507585946089499 1106 86-87 0.35198911114828135 1106 88-89 0.18715069475920743 1106 90-91 0.04056718772119794 1106 92-93 0.0073495628248387845 1106 94-95 -0.20571913323912128 1106 96-97 0.018254014675324726 1106 98-99 0.018458083728557284 1106 100-101 -0.04733611775395019 1107 1 0.17036479840358254 1107 2 0.11458134017092902 1107 3 0.1370715568628924 1107 4 0.010830213326876503 1107 5 0.09358108158764367 1107 6 0.11636230388128155 1107 7 -0.05125842743093756 1107 8 0.10515917408343256 1107 9 0.1268195517944548 1107 10-11 0.04716279403921675 1107 12-13 0.01630966238888476 1107 14-15 0.090918488801357 1107 16-17 0.18846670989922387 1107 18-19 0.1925593421220455 1107 20-21 0.3156281802552243 1107 22-23 0.2522932495591945 1107 24-25 0.32280766067459865 1107 26-27 0.34990350913284374 1107 28-29 0.42259441431095013 1107 30-31 0.4483929427325961 1107 32-33 0.4352873189682285 1107 34-35 0.42926705510395635 1107 36-37 0.4418421853686638 1107 38-39 0.4466094909379379 1107 40-41 0.5233676462030274 1107 42-43 0.578620205879794 1107 44-45 0.496598600722443 1107 46-47 0.5142571957758477 1107 48-49 0.5861337460693363 1107 50-51 0.4402403065333971 1107 52-53 0.38075705187941367 1107 54-55 0.4823383429715662 1107 56-57 0.45944976140761185 1107 58-59 0.37314741130714424 1107 60-61 0.3641528052483025 1107 62-63 0.4188502286823734 1107 64-65 0.3523433449331037 1107 66-67 0.4712710450964437 1107 68-69 0.49977562301434375 1107 70-71 0.5096109323855131 1107 72-73 0.6431443740334188 1107 74-75 0.48115672817329624 1107 76-77 0.5529343466947836 1107 78-79 0.43776587128749966 1107 80-81 0.48416244860606383 1107 82-83 0.4952506588234442 1107 84-85 0.3756537692364077 1107 86-87 0.5610883551482182 1107 88-89 0.6804742659295648 1107 90-91 0.7607596717288878 1107 92-93 0.716545206001193 1107 94-95 0.7617140989798585 1107 96-97 0.7279823855467065 1107 98-99 0.8440331170404107 1107 100-101 0.6404232824755205 1108 1 0.2606935764047833 1108 2 0.21237112888378107 1108 3 0.19353803653466883 1108 4 0.2256702643875812 1108 5 0.2148459867453738 1108 6 0.201269937798223 1108 7 0.05882776952847024 1108 8 0.13689109684344203 1108 9 0.21749584997872518 1108 10-11 0.20996831508467295 1108 12-13 0.1347700711306814 1108 14-15 0.16771617996948152 1108 16-17 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-0.9269535889942162 2316 56-57 -0.922681656663535 2316 58-59 -0.9946759672141141 2316 60-61 -0.7156262553567743 2316 62-63 -0.7270809228125117 2316 64-65 -0.8111208460729671 2316 66-67 -0.9459501373441981 2316 68-69 -0.9656454730444466 2316 70-71 -0.9629749037769031 2316 72-73 -0.9474544782334853 2316 74-75 -1.0558109126887274 2316 76-77 -1.0929045403106272 2316 78-79 -0.9278631294849831 2316 80-81 -1.0611066742156154 2316 82-83 -1.0352398136295697 2316 84-85 -1.2174046437834143 2316 86-87 -1.242947442235245 2316 88-89 -1.3438190051097543 2316 90-91 -1.225479838216632 2316 92-93 -1.3162394941933968 2316 94-95 -1.4294435262312604 2316 96-97 -1.4278729263955086 2316 98-99 -1.311965003612439 2316 100-101 -1.125812558563286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6541.0 3 1695.0 4 1789.0 5 1946.0 6 2247.0 7 2694.0 8 3263.0 9 4212.0 10 5317.0 11 6608.0 12 8312.0 13 10393.0 14 12295.0 15 14592.0 16 17263.0 17 22600.0 18 20563.0 19 16327.0 20 15887.0 21 16701.0 22 18354.0 23 20100.0 24 22082.0 25 24816.0 26 28125.0 27 32708.0 28 38111.0 29 45255.0 30 54619.0 31 66165.0 32 83287.0 33 108462.0 34 148442.0 35 217303.0 36 329764.0 37 462567.0 38 362052.0 39 44284.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.613695447563536 18.47325780345203 14.770488763239015 23.142557985745427 2 15.39348132588929 19.33695956025467 41.019427832946235 24.250131280909795 3 19.52215558640583 23.52817197033961 29.32050918047236 27.629163262782203 4 12.841649043469777 16.36396575931881 36.870442501924906 33.923942695286506 5 13.981265799849124 36.92425828909521 34.17602099038812 14.918454920667553 6 32.63213477232685 35.78138533042189 17.152556432513634 14.43392346473763 7 28.892728214236097 32.453951686344055 20.121718324077868 18.53160177534198 8 26.19812341171901 35.275775220465164 19.263246633584554 19.262854734231276 9 26.01669205992839 17.067537657720894 20.09522146567381 36.820548816676904 10-11 25.17942605873733 26.495169972667696 26.7956270934492 21.52977687514577 12-13 26.085485613704883 23.670923302138338 27.396677471636956 22.846913612519824 14-15 22.81968550894308 25.161222335174806 25.83916282612156 26.179929329760558 16-17 22.30615508290127 28.382046956338463 26.55542762284081 22.75637033791946 18-19 22.198844695856277 26.923246086750147 28.907120601472325 21.97078861592125 20-21 22.803830106009528 25.984638635551953 29.421498941646853 21.790032316791667 22-23 22.561247657343657 25.881499639742078 29.176362638303004 22.38089006461126 24-25 22.367612346176983 26.06875171785643 29.244940300269324 22.318695635697264 26-27 22.051342239425388 26.405239745259436 29.150361779595453 22.393056235719726 28-29 22.127127891108763 26.68365706028592 29.041095950068797 22.14811909853652 30-31 22.29466094280039 26.272964313229686 28.985173664930837 22.447201079039093 32-33 22.013644259331077 26.223052217633104 29.06087001588107 22.702433507154755 34-35 22.585185823950024 26.376182549699536 28.869891441915808 22.168740184434636 36-37 22.565448263938336 26.132271406317297 28.82813496890301 22.474145360841355 38-39 22.173850764255974 26.529132374524828 28.739721065182174 22.557295796037025 40-41 22.674622384250885 26.272263883210485 28.709725594795692 22.343388137742938 42-43 22.379901208444085 26.21372327479422 28.715927471584912 22.69044804517678 44-45 22.41157678044627 26.570322637937437 28.607938555270522 22.410162026345766 46-47 22.64891015653399 26.71902460217851 28.07202905610749 22.56003618518001 48-49 22.499134769217374 26.736654223305727 28.170847708498854 22.593363298978048 50-51 22.658382940859063 26.952269902088034 27.985348575353992 22.403998581698907 52-53 22.761963240660467 26.83049569887301 27.87046708926637 22.537073971200154 54-55 22.63316021147262 26.875128285105387 27.958088562268692 22.533622941153293 56-57 22.580252669502354 27.053206341246177 27.82154938878637 22.544991600465096 58-59 22.58353225203687 26.768736792411147 27.8320041493785 22.815726806173483 60-61 22.46539101906767 26.915631772937136 27.979863786486657 22.639113421508537 62-63 22.41434886068004 26.905506733102058 28.12976448779736 22.550379918420543 64-65 22.251339668554465 26.922266575541638 28.00077049986723 22.82562325603667 66-67 22.30601445405117 26.914630733075384 27.890015839996448 22.889338972877 68-69 22.474788915523984 26.717655442055317 28.07611795222485 22.73143769019585 70-71 22.482424483074954 26.747396272122597 27.796223707475132 22.97395553732732 72-73 22.45991458400136 26.83148179712753 27.723062167251918 22.985541451619188 74-75 22.33654048968687 27.08556405239272 27.685738584504954 22.89215687341546 76-77 22.472792711378446 26.84102054457455 27.70976454412705 22.976422199919945 78-79 22.494662943295637 26.929735844802373 27.729799221330552 22.845801990571434 80-81 22.557180385098384 26.948943014405824 27.5196042370834 22.97427236341239 82-83 22.529396247309805 26.967280622940997 27.639241487146272 22.864081642602923 84-85 22.576293644561375 26.945429171882918 27.579954451035732 22.898322732519976 86-87 22.70524693440255 26.903612732550368 27.429725098399878 22.961415234647202 88-89 22.666732845828026 27.011547175183857 27.30621859891314 23.01550138007498 90-91 22.579209617279613 26.965713098832023 27.336162815861996 23.118914468026368 92-93 22.639063636007435 26.715564417471715 27.502448878304897 23.142923068215953 94-95 22.55884646686061 26.679481669494848 27.47581456695262 23.285857296691916 96-97 22.6989668641998 26.51545415023127 27.477750582291332 23.307828403277593 98-99 22.6180786943869 26.48331533502005 27.288635550808248 23.60997041978481 100-101 23.064639494373807 25.97330666513795 27.481021688906754 23.481032151581488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 691.0 1 463.0 2 374.0 3 499.0 4 374.0 5 329.0 6 674.5 7 1603.5 8 2772.0 9 3283.0 10 2836.5 11 2264.5 12 2178.5 13 2305.5 14 2458.0 15 2584.0 16 2719.5 17 2963.5 18 3255.5 19 3544.0 20 3953.5 21 4632.5 22 5590.0 23 6919.5 24 8530.0 25 10768.0 26 13735.5 27 17068.0 28 19942.5 29 22639.5 30 25945.5 31 30030.0 32 35039.0 33 38297.0 34 40655.5 35 44282.5 36 49036.0 37 53481.5 38 56896.0 39 59978.5 40 63530.0 41 67757.5 42 70603.5 43 73462.5 44 77406.5 45 80288.0 46 82019.0 47 85483.5 48 87768.0 49 87302.0 50 86168.5 51 84335.0 52 82856.5 53 81892.5 54 81239.5 55 77021.0 56 70303.0 57 64084.5 58 59138.0 59 52905.0 60 43237.5 61 35386.0 62 29062.0 63 22110.0 64 16545.0 65 12839.5 66 9688.0 67 7351.5 68 5707.5 69 4324.5 70 3218.0 71 2213.5 72 1552.5 73 1170.5 74 826.0 75 536.0 76 380.0 77 256.0 78 156.5 79 104.5 80 71.0 81 51.0 82 39.0 83 26.5 84 16.5 85 11.5 86 10.0 87 8.5 88 7.5 89 6.5 90 3.5 91 4.0 92 4.0 93 2.0 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4927870648944379 2 0.04983142182320334 3 0.058796725662137735 4 0.06663048629809984 5 0.08003492116407943 6 0.10614745661728642 7 0.07076497107819095 8 0.05392238571087243 9 0.05248619626094605 10-11 0.07596571772262134 12-13 0.10910687730198321 14-15 0.05686004594935822 16-17 0.060972770283238324 18-19 0.0601458733272201 20-21 0.07516058121281412 22-23 0.06421507676867819 24-25 0.0873899519833994 26-27 0.0665216840670448 28-29 0.06734858102306303 30-31 0.07093905464787899 32-33 0.07035152260018183 34-35 0.07176595160389722 36-37 0.06719625789958598 38-39 0.04082259709184693 40-41 0.04500060276436004 42-43 0.043172725282635556 44-45 0.02297903119882215 46-47 0.029333081492435853 48-49 0.02924603970759183 50-51 0.02752696445692237 52-53 0.026830630178170178 54-55 0.02687415107059219 56-57 0.025938451883518943 58-59 0.02330543789198724 60-61 0.030094697109821055 62-63 0.020781226131510563 64-65 0.023370719230620257 66-67 0.017321315183960635 68-69 0.019323276235373173 70-71 0.04108372244637899 72-73 0.03788493685336114 74-75 0.03257538797787572 76-77 0.022739666290501087 78-79 0.015950407072667268 80-81 0.0179306076778688 82-83 0.022413259597335998 84-85 0.019105671773263114 86-87 0.010183888826750725 88-89 0.04141012913954408 90-91 0.052834363400322146 92-93 0.033815733411903054 94-95 0.05309548875485421 96-97 0.04358617376064467 98-99 0.033619889396004 100-101 0.16879578125877218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.53775782290321 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57926162948306 42.73081990441555 2 13.689407821433534 15.479368482943324 3 4.600800513058306 7.803568355963381 4 2.076844811783042 4.696805960173706 5 1.1473211047114482 3.2433481381640825 6 0.6890856119218043 2.3375613267649227 7 0.4608904527531489 1.8240398960452024 8 0.32082545160942394 1.4511001349213732 9 0.22418833906989757 1.1407595418947503 >10 1.1133632941833371 11.09841815908997 >50 0.06365595312205577 2.4530370256681757 >100 0.03081555822209139 3.229275691060825 >500 0.002385287350282048 0.8694477250921633 >1k 0.0010002817920532895 0.8984641181988162 >5k 1.5388950646973685E-4 0.7439855396037455 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9367 0.4076601993169831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7735 0.3366341028842601 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4620 0.20106652298969382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3056267726603495E-4 2 0.0 0.0 0.0 0.0 1.740835696880466E-4 3 0.0 0.0 0.0 0.0 1.740835696880466E-4 4 0.0 0.0 0.0 4.352089242201165E-5 1.740835696880466E-4 5 0.0 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 6 0.0 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 7 1.3056267726603495E-4 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 8 1.3056267726603495E-4 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 9 1.3056267726603495E-4 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 10-11 1.3056267726603495E-4 0.0 0.0 4.352089242201165E-5 2.611253545320699E-4 12-13 1.5232312347704076E-4 0.0 0.0 4.352089242201165E-5 5.440111552751456E-4 14-15 1.740835696880466E-4 0.0 0.0 4.352089242201165E-5 6.092924939081631E-4 16-17 1.9584401589905242E-4 0.0 0.0 8.70417848440233E-5 6.092924939081631E-4 18-19 2.1760446211005824E-4 0.0 0.0 1.5232312347704076E-4 6.092924939081631E-4 20-21 2.1760446211005824E-4 0.0 0.0 2.1760446211005824E-4 6.310529401191689E-4 22-23 2.1760446211005824E-4 0.0 0.0 3.0464624695408153E-4 6.528133863301747E-4 24-25 2.1760446211005824E-4 0.0 0.0 6.528133863301748E-4 6.528133863301747E-4 26-27 2.1760446211005824E-4 0.0 0.0 0.0011750640953943144 8.486574022292272E-4 28-29 2.611253545320699E-4 0.0 0.0 0.00343815050133892 8.70417848440233E-4 30-31 2.611253545320699E-4 0.0 0.0 0.011032546228979954 8.70417848440233E-4 32-33 2.611253545320699E-4 0.0 0.0 0.026700067500904145 9.574596332842562E-4 34-35 2.611253545320699E-4 0.0 0.0 0.04943973379140523 0.0010009805257062679 36-37 3.0464624695408153E-4 0.0 0.0 0.08691122216675726 0.0010009805257062679 38-39 3.6992758558709903E-4 0.0 0.0 0.14540330158194092 0.0010009805257062679 40-41 3.9168803179810485E-4 0.0 0.0 0.20715944792877544 0.0010009805257062679 42-43 4.1344847800911066E-4 0.0 0.0 0.2682192599968578 0.0010009805257062679 44-45 4.787298166421281E-4 0.0 0.0 0.34259646514607567 0.0010445014181282796 46-47 4.787298166421281E-4 0.0 0.0 0.4324018266588967 0.001109782756761297 48-49 4.787298166421281E-4 0.0 0.0 0.5282130713259554 0.0012185849878163261 50-51 4.787298166421281E-4 4.352089242201165E-5 0.0 0.623806711530904 0.0012621058802383379 52-53 4.787298166421281E-4 4.352089242201165E-5 0.0 0.7142866468762662 0.0013056267726603494 54-55 4.787298166421281E-4 4.352089242201165E-5 0.0 0.8137754069529848 0.0013491476650823611 56-57 5.222507090641398E-4 4.352089242201165E-5 0.0 0.9314559000621043 0.001370908111293367 58-59 5.222507090641398E-4 4.352089242201165E-5 0.0 1.0591244379820755 0.0013926685575043729 60-61 5.222507090641398E-4 4.352089242201165E-5 0.0 1.1936692769047246 0.0013926685575043729 62-63 5.657716014861514E-4 4.352089242201165E-5 0.0 1.3243189959556034 0.0014797103423483961 64-65 5.657716014861514E-4 4.352089242201165E-5 0.0 1.4623455062720134 0.0015014707885594018 66-67 6.092924939081631E-4 4.352089242201165E-5 0.0 1.613972295470302 0.0015449916809814135 68-69 6.092924939081631E-4 4.352089242201165E-5 0.0 1.7866631966008442 0.0015667521271924194 70-71 6.092924939081631E-4 4.352089242201165E-5 0.0 1.9636409056349546 0.0015885125734034253 72-73 6.310529401191689E-4 4.352089242201165E-5 0.0 2.143817400262083 0.0016537939120364427 74-75 6.745738325411806E-4 4.352089242201165E-5 0.0 2.3249948754149172 0.0017408356968804661 76-77 6.963342787521864E-4 4.352089242201165E-5 0.0 2.5197508690034196 0.0018061170355134835 78-79 6.963342787521864E-4 4.352089242201165E-5 0.0 2.7353533700620654 0.001871398374146501 80-81 9.139387408622446E-4 4.352089242201165E-5 0.0 2.969778657093231 0.0018931588203575066 82-83 9.574596332842562E-4 4.352089242201165E-5 0.0 3.2083601893506986 0.0019366797127795183 84-85 9.574596332842562E-4 4.352089242201165E-5 0.0 3.451576696651111 0.0019584401589905244 86-87 9.79220079495262E-4 4.352089242201165E-5 0.0 3.70230055789432 0.002132523728678571 88-89 0.0010009805257062679 4.352089242201165E-5 0.0 3.96753863676027 0.0023066072983666175 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4400 0.0 53.31158 1 GTATCAA 7660 0.0 40.60177 1 ATCAACG 9455 0.0 32.568726 3 TCAACGC 9495 0.0 32.481567 4 CAACGCA 9665 0.0 31.811907 5 TATCAAC 9700 0.0 31.746115 2 AACGCAG 10155 0.0 30.370508 6 ACGCAGA 11585 0.0 26.509048 7 CGCAGAG 11665 0.0 26.163088 8 TGGTATC 2110 0.0 23.647985 2 GCAGAGT 13000 0.0 23.36511 9 GAGTACT 7980 0.0 22.931635 12-13 GTGGTAT 2260 0.0 22.83011 1 CAGAGTA 12290 0.0 21.310839 10-11 TACATGG 7070 0.0 21.172806 2 GTACTTT 8605 0.0 21.086107 14-15 GTACATG 7355 0.0 21.045296 1 AGAGTAC 11230 0.0 20.825058 10-11 AGTACTT 8435 0.0 19.778769 12-13 GTACCGT 170 7.931042E-5 19.567549 6 >>END_MODULE