FastQCFastQC Report
Thu 26 May 2016
SRR938233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297747
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.3968234971039022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77910.33907127285989275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47060.2048093197379868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45050.052.5165561
GTATCAA79850.041.6471021
ATCAACG99850.033.1090853
TCAACGC100850.032.8735544
TATCAAC101300.032.8284382
CAACGCA102900.032.172485
AACGCAG107150.030.896396
ACGCAGA120100.027.367197
CGCAGAG120000.027.2316728
GGACCGA8350.025.5972446
GTGGTAT22750.024.8500461
AGGACCG9450.024.1255135
TGGTATC22200.023.967722
GCAGAGT137850.023.429849
GAGTACT83400.022.89428912-13
GTACATG73150.021.3669851
AGAGTAC117950.021.28208510-11
CAGAGTA126900.021.2595410-11
GTACTTT88700.021.23179614-15
TACATGG70950.021.159062
Produced by FastQC (version 0.11.5)