##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938233_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49793254000549 34.0 31.0 34.0 31.0 34.0 2 32.71692009607672 34.0 31.0 34.0 31.0 34.0 3 32.77536430251024 34.0 31.0 34.0 31.0 34.0 4 36.2351050833708 37.0 35.0 37.0 35.0 37.0 5 36.19658604711485 37.0 35.0 37.0 35.0 37.0 6 36.23283764487561 37.0 37.0 37.0 35.0 37.0 7 36.19880822388192 37.0 37.0 37.0 35.0 37.0 8 36.19109501611796 37.0 37.0 37.0 35.0 37.0 9 37.90037197306753 39.0 38.0 39.0 35.0 39.0 10-11 37.88562774752834 39.0 38.0 39.0 35.0 39.0 12-13 37.83257490924806 39.0 38.0 39.0 35.0 39.0 14-15 39.16117897227153 40.5 38.5 41.0 36.0 41.0 16-17 39.11221144016291 40.0 38.0 41.0 36.0 41.0 18-19 39.13559586847464 40.0 38.5 41.0 36.0 41.0 20-21 39.05952657102806 40.0 39.0 41.0 36.0 41.0 22-23 38.92179317392211 40.0 38.0 41.0 35.0 41.0 24-25 38.89063221494794 40.0 38.0 41.0 35.0 41.0 26-27 38.862443950530675 40.0 38.0 41.0 35.0 41.0 28-29 38.806859501938206 40.0 38.0 41.0 35.0 41.0 30-31 38.61866645892694 40.0 38.0 41.0 34.5 41.0 32-33 38.44994052870051 40.0 38.0 41.0 34.0 41.0 34-35 38.2600449483777 40.0 38.0 41.0 34.0 41.0 36-37 38.135403070921214 40.0 38.0 41.0 34.0 41.0 38-39 38.13443494866928 40.0 38.0 41.0 34.0 41.0 40-41 38.07555575091601 40.0 38.0 41.0 33.5 41.0 42-43 37.92421685242109 40.0 38.0 41.0 33.0 41.0 44-45 37.72614739568804 40.0 37.5 41.0 33.0 41.0 46-47 37.42094016443063 40.0 37.0 41.0 32.5 41.0 48-49 37.096014922443594 40.0 37.0 41.0 31.5 41.0 50-51 36.81036772107635 40.0 36.0 41.0 31.0 41.0 52-53 36.401332914372205 39.0 36.0 41.0 30.0 41.0 54-55 36.190316644956994 39.0 35.0 41.0 30.0 41.0 56-57 35.95070714922052 39.0 35.0 40.5 29.0 41.0 58-59 35.62203497600041 38.5 35.0 40.0 28.5 41.0 60-61 35.273179336106196 38.0 35.0 40.0 28.0 41.0 62-63 34.90898366965554 37.5 34.0 40.0 27.5 41.0 64-65 34.52281299899423 37.0 34.0 40.0 26.5 41.0 66-67 34.1086570888788 36.5 34.0 39.0 26.0 41.0 68-69 33.65204089048969 36.0 33.0 39.0 26.0 40.5 70-71 33.145103007424225 35.5 33.0 38.5 24.5 40.0 72-73 32.72709963281423 35.0 32.0 38.0 24.0 40.0 74-75 32.27885184922448 35.0 32.0 37.0 22.5 39.0 76-77 30.98126273258109 34.0 30.5 35.5 21.5 38.5 78-79 31.6994153403312 35.0 32.0 36.0 23.0 39.0 80-81 31.538728589352964 35.0 32.0 36.0 22.0 37.5 82-83 31.160980734606554 35.0 31.0 35.5 20.0 37.0 84-85 30.774460155970175 34.0 31.0 35.0 19.0 37.0 86-87 30.344547724357817 34.0 31.0 35.0 17.5 36.0 88-89 29.880729035877316 34.0 30.0 35.0 11.0 36.0 90-91 29.533807899651265 34.0 30.0 35.0 7.0 35.5 92-93 29.12428478853416 34.0 29.0 35.0 2.0 35.0 94-95 28.70432188574286 34.0 29.0 35.0 2.0 35.0 96-97 28.122545911277438 34.0 29.0 35.0 2.0 35.0 98-99 27.430187266048 33.5 27.5 35.0 2.0 35.0 100-101 24.992494822101825 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.036822871442723226 1101 2 0.04633453338067284 1101 3 8.057110689279057E-4 1101 4 0.0036637456265324886 1101 5 -0.02984571772930167 1101 6 -0.02894103179029628 1101 7 0.02313463742617472 1101 8 -0.017833242353184175 1101 9 -0.026379971567983773 1101 10-11 -0.021511892876276306 1101 12-13 -0.009116908494050335 1101 14-15 -0.022707057957262577 1101 16-17 -0.034136789687529756 1101 18-19 -0.03329049416707619 1101 20-21 0.11767223158867068 1101 22-23 0.13388806271115783 1101 24-25 0.06263806609037204 1101 26-27 -0.004533037115024285 1101 28-29 0.02120166664367673 1101 30-31 -0.046085117182627755 1101 32-33 -0.11929834526412009 1101 34-35 -0.16754090029986202 1101 36-37 0.03934325322128274 1101 38-39 0.01142056129048541 1101 40-41 0.07503804168847239 1101 42-43 0.006487603800572117 1101 44-45 0.058757291124315714 1101 46-47 0.0830145682012855 1101 48-49 -0.13139345853723228 1101 50-51 -0.03072320331165912 1101 52-53 -0.06784678360521212 1101 54-55 -0.07165991266878535 1101 56-57 -0.16012802772485202 1101 58-59 -0.133933385044692 1101 60-61 -0.11121891565375819 1101 62-63 -0.07142951029074851 1101 64-65 -0.11686650114114627 1101 66-67 -0.4958258274203189 1101 68-69 -0.34571950043302024 1101 70-71 -0.31504127771270873 1101 72-73 -0.4551707536030136 1101 74-75 -0.1928334669521945 1101 76-77 -0.30315323283199547 1101 78-79 -0.2775057868263744 1101 80-81 -0.25587683832425157 1101 82-83 -0.1698272318309435 1101 84-85 -0.0635533156274164 1101 86-87 0.09040949982523472 1101 88-89 0.08982548134580881 1101 90-91 0.06506801549391028 1101 92-93 0.25718599954713994 1101 94-95 0.10509948157831062 1101 96-97 0.17858780718242073 1101 98-99 0.039742675513998904 1101 100-101 0.24919616873753725 1102 1 0.09240567826209656 1102 2 0.1022458753566724 1102 3 0.13891316182503033 1102 4 0.06667778751878473 1102 5 0.05255059555469899 1102 6 0.036112837769735506 1102 7 0.019283414995285852 1102 8 0.10449887520464074 1102 9 0.06993229186987548 1102 10-11 0.08879731451148132 1102 12-13 0.1164644065629119 1102 14-15 0.17672453062204596 1102 16-17 0.12679050024920002 1102 18-19 0.1844263667218513 1102 20-21 0.14260887511456133 1102 22-23 0.23349848906640602 1102 24-25 0.1808768483953287 1102 26-27 -0.0276729429320568 1102 28-29 0.05302962279237988 1102 30-31 0.03972824401430586 1102 32-33 0.011080820786411039 1102 34-35 0.09550371512183631 1102 36-37 0.2164521481857946 1102 38-39 0.07503945095456999 1102 40-41 0.17693518564745858 1102 42-43 0.13827797274793596 1102 44-45 0.08916221016200865 1102 46-47 0.12327087339459553 1102 48-49 0.1599521914987747 1102 50-51 0.12334295667692174 1102 52-53 0.14802839127515455 1102 54-55 0.22283939258684882 1102 56-57 0.21960910506436448 1102 58-59 0.15187482989764334 1102 60-61 0.12404591343087645 1102 62-63 0.07164320758962361 1102 64-65 0.19653139271616027 1102 66-67 0.16070890621540457 1102 68-69 0.2615015199107589 1102 70-71 0.22767925829685254 1102 72-73 0.1266848772355118 1102 74-75 0.23386682191119235 1102 76-77 0.23313654777036064 1102 78-79 0.12439476625485213 1102 80-81 0.240821741635191 1102 82-83 0.2717181558546571 1102 84-85 0.2737506679862136 1102 86-87 0.21678077582060595 1102 88-89 -0.14706497791072692 1102 90-91 0.04751071021179243 1102 92-93 0.19044331162579198 1102 94-95 0.10072277661004847 1102 96-97 0.18722178926954314 1102 98-99 0.09008661686319641 1102 100-101 0.3798033334096509 1103 1 0.0827298671587684 1103 2 0.13711758608366154 1103 3 0.13984506553812537 1103 4 0.04576696207928421 1103 5 0.09631620178646472 1103 6 0.09265309790340126 1103 7 0.10333397760848584 1103 8 0.1161672352855021 1103 9 0.0881254504765181 1103 10-11 0.05056875197821853 1103 12-13 0.07785834939914338 1103 14-15 0.1971995911537121 1103 16-17 0.2084725969694503 1103 18-19 0.18968773100363734 1103 20-21 0.20077502323887586 1103 22-23 0.2109957491012011 1103 24-25 0.2037186919238536 1103 26-27 0.20766598182355267 1103 28-29 0.2300647174868402 1103 30-31 0.28499331066750244 1103 32-33 0.31734172016815876 1103 34-35 0.31742827509709315 1103 36-37 0.320986922078184 1103 38-39 0.36032336604404946 1103 40-41 0.5046521688037657 1103 42-43 0.3523335209408245 1103 44-45 0.34455673359739336 1103 46-47 0.3093403854178334 1103 48-49 0.34316446149421154 1103 50-51 0.3724824708668919 1103 52-53 0.41645084447487335 1103 54-55 0.46993702790746994 1103 56-57 0.4410124931549291 1103 58-59 0.38029147710582123 1103 60-61 0.40035441773593305 1103 62-63 0.5530596170887279 1103 64-65 0.466061191546963 1103 66-67 0.4698180020584459 1103 68-69 0.43087039180240083 1103 70-71 0.48523199079420465 1103 72-73 0.23885326781887528 1103 74-75 0.33293260421596926 1103 76-77 0.41273332586578704 1103 78-79 0.345963814429858 1103 80-81 0.4269741362911539 1103 82-83 0.5737802294771548 1103 84-85 0.6577281298002475 1103 86-87 0.580563274121932 1103 88-89 0.49464244639576194 1103 90-91 0.5936952692193849 1103 92-93 0.636354189655794 1103 94-95 0.422267527972668 1103 96-97 0.5848523767475342 1103 98-99 0.7169145049758505 1103 100-101 0.750942086089136 1104 1 0.0945122945302046 1104 2 0.11577106796245573 1104 3 0.0654207185749982 1104 4 0.016904957345680316 1104 5 -0.014927886127871659 1104 6 -0.012616091515688765 1104 7 0.07280457765810411 1104 8 0.02426477436138441 1104 9 -0.011874943334348131 1104 10-11 0.061612193255115244 1104 12-13 0.036365648683592156 1104 14-15 0.12855244962990042 1104 16-17 0.11043554724295035 1104 18-19 0.09999966859248843 1104 20-21 0.01618307037016109 1104 22-23 0.09990800410161427 1104 24-25 0.14497333396946743 1104 26-27 0.12460063678616251 1104 28-29 0.06817832404614421 1104 30-31 0.07385712693110236 1104 32-33 0.08683555505901097 1104 34-35 0.1256709334779842 1104 36-37 0.1607494147434565 1104 38-39 0.2076801074953991 1104 40-41 0.11330517810952045 1104 42-43 0.20429135167303514 1104 44-45 0.28149245184933847 1104 46-47 0.20380813033866474 1104 48-49 0.2286572567243752 1104 50-51 0.15694958569033446 1104 52-53 0.18657261079537335 1104 54-55 0.07540174442271308 1104 56-57 0.04852679833472706 1104 58-59 0.10168170823342138 1104 60-61 0.1109124871175382 1104 62-63 -0.044834282387107294 1104 64-65 -0.11416142973693155 1104 66-67 0.05516961609228588 1104 68-69 0.0555550477495359 1104 70-71 0.06479005623769041 1104 72-73 0.05093105334159276 1104 74-75 -0.08201212442865824 1104 76-77 -0.05879670812009152 1104 78-79 0.06808298947484914 1104 80-81 0.07149858997702552 1104 82-83 0.10488853140407173 1104 84-85 0.20598177550436247 1104 86-87 -0.06120214061997942 1104 88-89 -0.1472531228381584 1104 90-91 0.04793464321762997 1104 92-93 0.14739605568921377 1104 94-95 0.014758145428729108 1104 96-97 0.10490443854433451 1104 98-99 0.21606997756858703 1104 100-101 0.2987589955402008 1105 1 0.18391119409918844 1105 2 0.1710754360300868 1105 3 0.10678140107351908 1105 4 0.08294156721159851 1105 5 0.06915493235141668 1105 6 0.05971700903986488 1105 7 0.07795645403975726 1105 8 0.09137594280205974 1105 9 0.08144411197335444 1105 10-11 0.13687595865069824 1105 12-13 0.13063861102548202 1105 14-15 0.22004468122748477 1105 16-17 0.19967932340264838 1105 18-19 0.17448021830389138 1105 20-21 0.2590570308394433 1105 22-23 0.22448600170859834 1105 24-25 0.20248668162530237 1105 26-27 0.13915499336317794 1105 28-29 0.1218243357621347 1105 30-31 0.18093787116762883 1105 32-33 0.09106095363535616 1105 34-35 0.17736780194964297 1105 36-37 0.2372979059134721 1105 38-39 0.20210437539445536 1105 40-41 0.20422955053617642 1105 42-43 0.23091623695214025 1105 44-45 0.2997497543255321 1105 46-47 0.23315944390218846 1105 48-49 0.18433014018870608 1105 50-51 0.30733200103409075 1105 52-53 0.34458646774526613 1105 54-55 0.41419516229738207 1105 56-57 0.3456478042312696 1105 58-59 0.38983613627468827 1105 60-61 0.3292543383384299 1105 62-63 0.3231032471734423 1105 64-65 0.4024310297456424 1105 66-67 0.35051939806698584 1105 68-69 0.3413720606916826 1105 70-71 0.3358581133438676 1105 72-73 0.33012705816320675 1105 74-75 0.32902728766659095 1105 76-77 0.29451622379759357 1105 78-79 0.38821118776000674 1105 80-81 0.29876624587512524 1105 82-83 0.45969468830222837 1105 84-85 0.5242218049796641 1105 86-87 0.4493190073154878 1105 88-89 0.16752415182331148 1105 90-91 0.1354677992247062 1105 92-93 0.33337215815888044 1105 94-95 0.31435167390990415 1105 96-97 0.4220009657074293 1105 98-99 0.46229345461625115 1105 100-101 0.5569369830650928 1106 1 -0.1097918975084724 1106 2 8.744400700848587E-4 1106 3 -0.039079240175745156 1106 4 -0.04261362302607097 1106 5 -0.05225697116004824 1106 6 -0.024922861075083347 1106 7 0.010791346682367475 1106 8 -0.09915632697129695 1106 9 -0.05137128627244181 1106 10-11 0.018893577802927553 1106 12-13 -0.04789969473686284 1106 14-15 -0.19888354156728383 1106 16-17 -0.10821557997643083 1106 18-19 -0.0475687810079819 1106 20-21 0.06390975358111461 1106 22-23 0.04108991775466819 1106 24-25 0.07210244560926071 1106 26-27 -0.034787581090142794 1106 28-29 0.016242173426178397 1106 30-31 0.1104474416071497 1106 32-33 0.006259418233391045 1106 34-35 0.048752973405768785 1106 36-37 0.014800462734541497 1106 38-39 -0.07366950851496057 1106 40-41 0.03658082938315488 1106 42-43 -0.09783785287047664 1106 44-45 -0.03402250602068335 1106 46-47 -0.011013877288363005 1106 48-49 0.046107956438007136 1106 50-51 0.10980447974664997 1106 52-53 0.07416208636504251 1106 54-55 -0.2641420165724355 1106 56-57 0.014710960257730221 1106 58-59 0.036838072621115714 1106 60-61 -0.016471515159722117 1106 62-63 0.017645325247052313 1106 64-65 -0.05412556146994518 1106 66-67 -0.12200223125724108 1106 68-69 0.013508311977361132 1106 70-71 -0.1344363902545993 1106 72-73 -0.17458305724652945 1106 74-75 -0.038465551052183855 1106 76-77 0.022206414188019608 1106 78-79 0.08150813397729095 1106 80-81 -0.13902680655461808 1106 82-83 -0.2867106685834422 1106 84-85 -0.11646900224839385 1106 86-87 -0.012483431520841748 1106 88-89 0.08831707186573468 1106 90-91 -0.05467658297022027 1106 92-93 0.09144014704998682 1106 94-95 -0.014297306198535864 1106 96-97 0.09365658554922973 1106 98-99 0.23563308483372936 1106 100-101 0.09202871584055927 1107 1 0.10413656103028046 1107 2 0.12766991838986996 1107 3 0.02775926850105037 1107 4 0.08395552679257179 1107 5 0.05576895882131794 1107 6 0.07539375433586315 1107 7 0.07653628354265152 1107 8 0.005621195255521627 1107 9 0.013496278373509085 1107 10-11 0.07900475120094086 1107 12-13 0.06794338766173524 1107 14-15 0.1567249869656493 1107 16-17 0.08316376650002866 1107 18-19 0.09545904377603165 1107 20-21 0.0894174646420538 1107 22-23 0.1398453729526281 1107 24-25 0.09089216442752956 1107 26-27 0.1710384568163903 1107 28-29 0.14346865386082186 1107 30-31 0.11226662678961219 1107 32-33 0.13303123334954847 1107 34-35 0.23748675625265747 1107 36-37 0.19456676458620592 1107 38-39 0.13091530267713125 1107 40-41 0.2004756704554964 1107 42-43 0.22105813206889735 1107 44-45 0.2416979119186422 1107 46-47 0.2869310913557115 1107 48-49 0.3028022854770427 1107 50-51 0.2253699779225684 1107 52-53 0.2781507769254361 1107 54-55 0.4031570077351816 1107 56-57 0.3297136933140905 1107 58-59 0.3438226470065331 1107 60-61 0.32164868343655684 1107 62-63 0.40565934504031276 1107 64-65 0.4425664798350013 1107 66-67 0.36150294408621875 1107 68-69 0.5414483558809025 1107 70-71 0.5300764882911793 1107 72-73 0.5045316769833832 1107 74-75 0.3975561317770655 1107 76-77 0.30625505735214986 1107 78-79 0.41506596213179137 1107 80-81 0.5004498786409535 1107 82-83 0.5515754343925323 1107 84-85 0.6152779359426219 1107 86-87 0.6519098212353533 1107 88-89 0.6266099856530865 1107 90-91 0.5472851053426062 1107 92-93 0.6136150137589134 1107 94-95 0.536062880941607 1107 96-97 0.8065140003398703 1107 98-99 0.986822619236186 1107 100-101 1.0346173306088389 1108 1 0.011264994072874401 1108 2 0.036120977387184894 1108 3 -0.033200211670234125 1108 4 0.004330711973011603 1108 5 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 10.0 5 32.0 6 68.0 7 170.0 8 420.0 9 840.0 10 1586.0 11 2463.0 12 3717.0 13 5443.0 14 7583.0 15 10264.0 16 13126.0 17 18172.0 18 17934.0 19 12922.0 20 11077.0 21 10939.0 22 11856.0 23 12771.0 24 14569.0 25 16955.0 26 20124.0 27 24542.0 28 29913.0 29 37129.0 30 45514.0 31 56073.0 32 70794.0 33 92499.0 34 128292.0 35 193690.0 36 324337.0 37 543987.0 38 497472.0 39 60455.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76027778515215 18.918531935848463 15.203066307996485 23.1181239710029 2 15.645806346022592 19.609850542532 40.49676674606159 24.24757636538381 3 19.559335002631038 23.48230067118651 29.655728022569903 27.302636303612548 4 13.131428307816309 16.70371019959987 36.635582594602454 33.52927889798137 5 13.881479450504733 37.006195204428685 34.324609562854015 14.78771578221256 6 32.711738933833885 36.019065632552234 16.876314058945567 14.392881374668317 7 28.89795961000058 32.41031323291903 20.099253747257638 18.59247340982275 8 26.19587280697173 35.56692326707681 19.07486325670138 19.16234066925008 9 25.787362679927266 17.041702825479362 20.053365620215395 37.11756887437797 10-11 25.11516716157175 26.559712622843158 26.781386288394675 21.543733927190416 12-13 26.025189022116013 23.641549744162436 27.40262526727268 22.93063596644887 14-15 22.68258864008962 25.202034862846084 25.846013508014593 26.26936298904971 16-17 22.233914860763306 28.43989882240913 26.609014415021353 22.717171901806207 18-19 22.145040337339143 26.94265295526444 28.915019799830006 21.997286907566412 20-21 22.688551002351435 25.99058991264051 29.499353061934148 21.821506023073905 22-23 22.46870521428164 25.90531072393958 29.287471597177582 22.338512464601195 24-25 22.3175548179351 26.07566889077151 29.310112579122343 22.29666371217105 26-27 21.9817428043352 26.44570593300094 29.194384155912672 22.378167106751185 28-29 22.128081634802463 26.672971397398015 29.068841610178325 22.1301053576212 30-31 22.232277966199064 26.25556686615193 29.07038938577659 22.441765781872416 32-33 22.018115646369527 26.206670692365858 29.085480826247554 22.689732835017054 34-35 22.534995463218554 26.416821937258998 28.928037657571267 22.12014494195118 36-37 22.51357025719946 26.159543224620396 28.922296132781355 22.404590385398787 38-39 22.141726497984752 26.53570193458887 28.80428265207718 22.518288915349196 40-41 22.64591841087414 26.245052045473738 28.792677331991534 22.31635221166059 42-43 22.293638176814877 26.212491662395855 28.795108750648783 22.698761410140484 44-45 22.393756186897456 26.502280434095248 28.682962538383517 22.42100084062378 46-47 22.54106360296926 26.720571655750536 28.18763949809085 22.55072524318936 48-49 22.458586606793524 26.678611700940095 28.22673688617589 22.636064806090488 50-51 22.604446877745897 26.9765774909074 28.043666252202705 22.375309379144003 52-53 22.73648926535428 26.789851101970758 27.94894302984619 22.52471660282877 54-55 22.581424325654652 26.922676865642735 27.96787461804977 22.528024190652843 56-57 22.525043009521937 27.056133682254835 27.902288633169796 22.516534675053432 58-59 22.508091621923562 26.830543136385337 27.855525434262347 22.805839807428754 60-61 22.413368399567055 27.028889603598653 27.9380845671869 22.61965742964739 62-63 22.354745757474603 26.938300866022235 28.10446493891625 22.602488437586906 64-65 22.19045438858151 26.978035440803534 27.96210809980385 22.869402070811105 66-67 22.23995940371155 26.990808822729395 27.817335851162028 22.951895922397025 68-69 22.42922505190968 26.774536381602626 28.016712936749617 22.779525629738078 70-71 22.466050439843897 26.801275336231534 27.685685151585442 23.046989072339123 72-73 22.387908677500175 26.892690970763972 27.63369944558735 23.085700906148503 74-75 22.303478146201474 27.136799656359035 27.55575352725953 23.003968670179965 76-77 22.434301948822043 26.885488263068126 27.64547184698751 23.034737941122327 78-79 22.470076122392936 27.00153672271142 27.69808860592572 22.830298548969928 80-81 22.466616211445388 27.0050619149976 27.47332495701224 23.054996916544773 82-83 22.51473835663298 27.016019177054385 27.57084551556177 22.898396950750865 84-85 22.54652305501467 27.031050477917272 27.476891888245436 22.94553457882262 86-87 22.58188551293219 27.01594678760893 27.352364969771326 23.049802729687556 88-89 22.579437104762036 27.142714456409557 27.226513989473595 23.051334449354812 90-91 22.472421850485453 27.08040020141504 27.301508480278258 23.145669467821246 92-93 22.47369423182587 26.846125808539924 27.41834086791898 23.26183909171522 94-95 22.37889985276882 26.840922869227988 27.41881503925367 23.36136223874952 96-97 22.53653742917245 26.675832168605197 27.4154916567167 23.372138745505648 98-99 22.412889669750413 26.720391757665222 27.223797920310634 23.642920652273727 100-101 22.45480028915281 26.589872601291614 27.42364585831251 23.531681251243068 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 680.0 1 465.0 2 330.0 3 424.0 4 350.5 5 331.0 6 692.0 7 1677.5 8 2774.0 9 3191.5 10 2726.0 11 2091.5 12 2013.5 13 2219.5 14 2415.0 15 2573.5 16 2765.5 17 2934.0 18 3145.5 19 3455.0 20 3886.5 21 4548.5 22 5495.0 23 6767.0 24 8409.5 25 10620.0 26 13525.0 27 16803.0 28 19596.0 29 22560.0 30 25871.0 31 29882.0 32 35230.5 33 38704.0 34 40674.0 35 44043.0 36 48981.0 37 53670.5 38 57141.5 39 60191.0 40 63667.5 41 67921.5 42 70543.0 43 73333.5 44 77706.0 45 80778.0 46 82321.5 47 86095.0 48 88333.0 49 87598.0 50 87146.5 51 85400.0 52 83466.5 53 82559.0 54 82466.5 55 78081.5 56 71212.0 57 64910.5 58 59901.0 59 53366.5 60 43120.0 61 35103.5 62 28524.0 63 21272.5 64 15484.5 65 11722.0 66 8739.0 67 6577.5 68 5017.5 69 3744.5 70 2628.5 71 1663.0 72 1164.0 73 898.5 74 625.5 75 427.0 76 285.5 77 173.5 78 107.0 79 63.5 80 32.5 81 21.0 82 11.5 83 8.0 84 7.0 85 3.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01614625108856632 3 0.007572635281430026 4 0.0 5 8.70417848440233E-5 6 0.0 7 0.0 8 4.7872981664212816E-4 9 5.657716014861514E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1760446211005827E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005157225752008381 26-27 0.02006313140654737 28-29 6.528133863301747E-5 30-31 0.0 32-33 4.134484780091106E-4 34-35 0.01799588901650182 36-37 0.0032640669316508736 38-39 0.01677730402868549 40-41 0.014492457176529878 42-43 0.007594395727641032 44-45 0.002110763282467565 46-47 2.1760446211005826E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.611253545320699E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.352089242201165E-5 84-85 0.0030029415771188036 86-87 5.004902628531339E-4 88-89 6.528133863301747E-5 90-91 1.740835696880466E-4 92-93 4.134484780091106E-4 94-95 0.0 96-97 2.1760446211005826E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2297747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.4978205528881 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7431911412128 40.18843197811143 2 14.544831003117253 15.853231799599351 3 5.064371867721586 8.279916877805572 4 2.2690944750173867 4.946428140681895 5 1.2215529738425595 3.3285987382159306 6 0.751400879110125 2.4569826163815573 7 0.5013549663140813 1.9125927091238841 8 0.33690791903399 1.4688597851489071 9 0.24689197139191607 1.2109566917578882 >10 1.2140566730416051 11.73757092934185 >50 0.06930373001776792 2.5945068584528146 >100 0.033445492896100555 3.475706894463527 >500 0.002397938188564257 0.8726971012397009 >1k 0.00103910654837773 0.9369492837457841 >5k 1.5986254590426616E-4 0.736569595929958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9118 0.3968234971039022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7791 0.33907127285989275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4706 0.2048093197379868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.70417848440233E-5 7 0.0 0.0 0.0 0.0 1.3056267726603495E-4 8 0.0 0.0 0.0 0.0 1.740835696880466E-4 9 0.0 0.0 0.0 4.352089242201165E-5 1.740835696880466E-4 10-11 0.0 0.0 0.0 4.352089242201165E-5 2.1760446211005824E-4 12-13 0.0 0.0 0.0 4.352089242201165E-5 4.1344847800911066E-4 14-15 0.0 0.0 0.0 4.352089242201165E-5 4.569693704311223E-4 16-17 2.1760446211005826E-5 0.0 0.0 1.5232312347704076E-4 4.787298166421281E-4 18-19 6.528133863301747E-5 0.0 0.0 1.740835696880466E-4 4.787298166421281E-4 20-21 8.70417848440233E-5 0.0 0.0 2.1760446211005824E-4 9.139387408622446E-4 22-23 8.70417848440233E-5 0.0 0.0 3.0464624695408153E-4 0.0010445014181282796 24-25 8.70417848440233E-5 0.0 0.0 6.528133863301748E-4 0.0010445014181282796 26-27 8.70417848440233E-5 0.0 0.0 0.0011750640953943144 0.0010445014181282796 28-29 8.70417848440233E-5 0.0 0.0 0.003394629608916909 0.001109782756761297 30-31 8.70417848440233E-5 0.0 0.0 0.010967264890346936 0.0011315432029723029 32-33 1.0880223105502913E-4 0.0 0.0 0.02648246303879409 0.0012621058802383379 34-35 1.5232312347704076E-4 0.0 0.0 0.04909156665202914 0.0013056267726603494 36-37 1.740835696880466E-4 0.0 0.0 0.08671537815085821 0.0013056267726603494 38-39 1.740835696880466E-4 0.0 0.0 0.14557738515162896 0.001370908111293367 40-41 1.740835696880466E-4 0.0 0.0 0.20755113596057356 0.0014361894499263844 42-43 1.9584401589905242E-4 0.0 0.0 0.2694160845384631 0.0014579498961373903 44-45 2.611253545320699E-4 0.0 0.0 0.34420673816569014 0.0014797103423483961 46-47 3.0464624695408153E-4 0.0 0.0 0.43481723618831836 0.0015232312347704079 48-49 3.481671393760932E-4 0.0 0.0 0.5311289711182301 0.0015885125734034253 50-51 3.481671393760932E-4 0.0 0.0 0.6266573299845457 0.0016973148044584544 52-53 3.481671393760932E-4 0.0 0.0 0.7177030369313941 0.0017843565893024777 54-55 3.481671393760932E-4 0.0 0.0 0.817931652179287 0.0018278774817244892 56-57 3.481671393760932E-4 0.0 0.0 0.9371353765231769 0.001849637927935495 58-59 3.481671393760932E-4 0.0 0.0 1.065347925598423 0.001871398374146501 60-61 3.481671393760932E-4 0.0 0.0 1.2009807868316225 0.001871398374146501 62-63 3.481671393760932E-4 0.0 0.0 1.3327185281930518 0.0019149192665685124 64-65 3.481671393760932E-4 0.0 0.0 1.471484893680636 0.0019584401589905244 66-67 3.9168803179810485E-4 0.0 0.0 1.6239821007273647 0.00198020060520153 68-69 3.9168803179810485E-4 0.0 0.0 1.797478138367714 0.0020019610514125357 70-71 4.1344847800911066E-4 0.0 0.0 1.975282744357843 0.0020237214976235414 72-73 4.352089242201165E-4 0.0 0.0 2.1560250105864567 0.0021325237286785705 74-75 4.352089242201165E-4 0.0 0.0 2.3378988200180437 0.0022413259597335996 76-77 4.352089242201165E-4 0.0 0.0 2.533024741192133 0.0022630864059446057 78-79 4.352089242201165E-4 0.0 0.0 2.7517607465051634 0.0023936490832106405 80-81 6.092924939081631E-4 0.0 0.0 2.9898853093922 0.0024154095294216466 82-83 6.528133863301747E-4 0.0 0.0 3.231600345904053 0.0024371699756326522 84-85 7.180947249631922E-4 0.0 0.0 3.478146201474749 0.002480690868054664 86-87 7.833760635962097E-4 0.0 0.0 3.732873984820783 0.0025459722066876814 88-89 7.833760635962097E-4 0.0 0.0 4.002638236498623 0.0026112535453206988 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4505 0.0 52.516556 1 GTATCAA 7985 0.0 41.647102 1 ATCAACG 9985 0.0 33.109085 3 TCAACGC 10085 0.0 32.873554 4 TATCAAC 10130 0.0 32.828438 2 CAACGCA 10290 0.0 32.17248 5 AACGCAG 10715 0.0 30.89639 6 ACGCAGA 12010 0.0 27.36719 7 CGCAGAG 12000 0.0 27.231672 8 GGACCGA 835 0.0 25.597244 6 GTGGTAT 2275 0.0 24.850046 1 AGGACCG 945 0.0 24.125513 5 TGGTATC 2220 0.0 23.96772 2 GCAGAGT 13785 0.0 23.42984 9 GAGTACT 8340 0.0 22.894289 12-13 GTACATG 7315 0.0 21.366985 1 AGAGTAC 11795 0.0 21.282085 10-11 CAGAGTA 12690 0.0 21.25954 10-11 GTACTTT 8870 0.0 21.231796 14-15 TACATGG 7095 0.0 21.15906 2 >>END_MODULE