FastQCFastQC Report
Thu 26 May 2016
SRR938232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92050.3907634639345062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75370.31995483190378854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44460.18873811631209286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.056.1477281
GTATCAA79650.042.0792661
ATCAACG99000.033.2867053
TCAACGC99950.033.01794
TATCAAC100800.032.7394752
CAACGCA102250.032.3217055
AACGCAG107000.030.9253836
ACGCAGA119200.027.6370187
CGCAGAG120650.027.1443798
GCAGAGT132550.024.3488259
GAGTACT84250.023.63966612-13
TGGTATC19000.023.2756652
GTGGTAT20650.023.0547921
GTACATG76800.022.5022891
TACATGG73900.022.3927572
CAGAGTA126100.022.25763110-11
GTACTTT89700.022.12223214-15
AGAGTAC119500.021.33909410-11
GGACCGA7900.021.0635016
ACATGGG78250.020.4794523
Produced by FastQC (version 0.11.5)