FastQCFastQC Report
Thu 26 May 2016
SRR938232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87350.3708113913599036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76950.3266621243863146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.19782267701627365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.056.879581
GTATCAA79950.039.391111
ATCAACG102150.030.5467913
TATCAAC103250.030.4532892
TCAACGC102400.030.4238934
CAACGCA104750.029.7413525
AACGCAG107900.028.8730936
TGGTATC20550.027.7354772
GTGGTAT22700.026.156961
ACGCAGA122150.025.3881267
CGCAGAG122850.025.2048078
GAGTACT86350.023.12724112-13
TACATGG73250.022.240922
GTACATG76550.022.1526741
GCAGAGT138400.022.0297669
GTGTAAG10200.021.8877331
GGACCGA8950.021.7556326
GTACTTT91800.021.70202314-15
CAGAGTA128700.021.6423810-11
GTAAGGT11500.021.0612074
Produced by FastQC (version 0.11.5)