FastQCFastQC Report
Thu 26 May 2016
SRR938231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263423
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98090.4333701654529445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78520.34690820054404325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.21926966369079046No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27370.12092304443314396No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24840.10974528402335755No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24380.10771296394885092No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24340.10753624046411121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.043.978841
GTATCAA97750.038.3747251
TATCAAC121650.030.7853832
ATCAACG121800.030.5523723
TCAACGC122950.030.2666054
CAACGCA123850.030.0082875
AACGCAG128350.029.0965796
ACGCAGA141600.026.0326147
CGCAGAG143950.025.4426298
GTACATG84000.024.9416241
TACATGG84150.024.2853742
ACATGGG83450.023.8626213
GCAGAGT157000.022.7821879
GAGTACT93350.021.75385712-13
AGAGTAC130350.021.15417510-11
TAAGGTG12750.020.8741265
GTACTTT96700.020.7309714-15
AGTACTT96700.019.99320412-13
CAGAGTA149600.019.9244810-11
GTGTAAG9700.019.680121
Produced by FastQC (version 0.11.5)