##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938231_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2263423 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98632027685501 33.0 31.0 34.0 30.0 34.0 2 32.14256106790467 34.0 31.0 34.0 30.0 34.0 3 32.236188728311056 34.0 31.0 34.0 30.0 34.0 4 35.75472194106007 37.0 35.0 37.0 35.0 37.0 5 35.768817406202906 37.0 35.0 37.0 35.0 37.0 6 35.805736267591165 37.0 36.0 37.0 35.0 37.0 7 35.743627240688106 37.0 36.0 37.0 35.0 37.0 8 35.713014756852786 37.0 35.0 37.0 35.0 37.0 9 37.36151218751422 39.0 37.0 39.0 34.0 39.0 10-11 37.262636502324135 39.0 37.0 39.0 34.0 39.0 12-13 37.135851981710886 39.0 37.0 39.0 33.5 39.0 14-15 38.321544625109844 40.0 38.0 41.0 33.5 41.0 16-17 38.15760355002136 40.0 38.0 41.0 33.0 41.0 18-19 38.34074209725712 40.0 38.0 41.0 34.0 41.0 20-21 38.33099866883035 40.0 38.0 41.0 34.0 41.0 22-23 38.22645855414565 40.0 38.0 41.0 34.0 41.0 24-25 38.09936344200797 40.0 38.0 41.0 33.0 41.0 26-27 37.90091732742842 40.0 38.0 41.0 33.0 41.0 28-29 37.69528696138548 40.0 38.0 41.0 32.5 41.0 30-31 37.43652512146426 40.0 37.0 41.0 32.0 41.0 32-33 37.11001213648532 40.0 36.5 41.0 31.0 41.0 34-35 36.816353151841255 39.0 36.0 41.0 30.5 41.0 36-37 36.51564731824321 39.0 35.5 41.0 30.0 41.0 38-39 36.18376193932818 39.0 35.0 40.5 30.0 41.0 40-41 36.017777940756105 39.0 35.0 40.0 29.0 41.0 42-43 35.7520403830835 39.0 35.0 40.0 27.5 41.0 44-45 35.38431548146325 38.5 35.0 40.0 26.5 41.0 46-47 34.92221162372212 38.0 34.0 40.0 24.5 41.0 48-49 34.42837198349579 38.0 33.5 40.0 23.5 41.0 50-51 33.58738534511667 37.5 32.5 39.5 21.5 40.5 52-53 33.55585191102149 38.0 33.0 39.5 20.5 40.5 54-55 33.528898486937706 38.0 33.0 40.0 19.0 41.0 56-57 33.054943331405575 37.5 32.5 40.0 14.0 41.0 58-59 32.59006800761502 37.0 31.5 40.0 9.0 41.0 60-61 32.0194238991121 36.0 31.0 39.5 6.5 41.0 62-63 31.397106506384358 36.0 30.5 39.0 2.0 41.0 64-65 30.780969354822318 35.0 29.5 39.0 2.0 40.0 66-67 30.091182028281942 35.0 28.5 38.0 2.0 40.0 68-69 29.382628876705766 34.5 28.0 38.0 2.0 40.0 70-71 28.549819896678613 34.0 26.0 37.0 2.0 39.5 72-73 27.746326912821864 34.0 25.5 36.5 2.0 39.0 74-75 26.91815361070379 33.0 22.5 36.0 2.0 39.0 76-77 26.08398275532236 32.5 20.0 35.0 2.0 37.5 78-79 25.244810183514083 32.0 15.5 35.0 2.0 37.0 80-81 24.442402502757993 31.0 7.5 35.0 2.0 36.5 82-83 23.612698112549 31.0 2.0 35.0 2.0 36.0 84-85 22.79669796586851 30.0 2.0 34.0 2.0 35.5 86-87 22.029950654384976 29.5 2.0 34.0 2.0 35.0 88-89 21.188651436342212 29.0 2.0 34.0 2.0 35.0 90-91 20.33269631880563 28.0 2.0 34.0 2.0 35.0 92-93 19.42331592459739 26.5 2.0 34.0 2.0 35.0 94-95 18.482991910924294 25.0 2.0 33.5 2.0 35.0 96-97 17.367804648092733 21.0 2.0 33.0 2.0 35.0 98-99 16.04979515539075 2.0 2.0 32.0 2.0 34.5 100-101 12.962290301017529 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48581482160232525 1101 2 0.43761367700603415 1101 3 0.3652046422421833 1101 4 0.273885245645495 1101 5 0.3407124756886759 1101 6 0.2733164504718104 1101 7 0.22556281452206406 1101 8 0.2387125751179937 1101 9 0.29048794386469723 1101 10-11 0.306925863678444 1101 12-13 0.36733086169533635 1101 14-15 0.49778222430410324 1101 16-17 0.7207455332089481 1101 18-19 0.6819529170780569 1101 20-21 0.5408898900987964 1101 22-23 0.6100389571723639 1101 24-25 0.601082307477256 1101 26-27 0.6491717883334971 1101 28-29 0.7565194754077851 1101 30-31 0.8715312566858202 1101 32-33 0.8610529107492724 1101 34-35 0.9703867672610755 1101 36-37 0.9458485611660237 1101 38-39 1.1952503568601642 1101 40-41 1.1990391687334636 1101 42-43 1.3182142789122864 1101 44-45 1.4885395663665406 1101 46-47 1.694510924897564 1101 48-49 1.7168914892617337 1101 50-51 1.8799853645918745 1101 52-53 1.953097306371724 1101 54-55 2.0022989786778496 1101 56-57 2.051468043664002 1101 58-59 2.4578429074999804 1101 60-61 2.506993532384797 1101 62-63 2.6690741005734786 1101 64-65 2.64144798343035 1101 66-67 2.8233248830789144 1101 68-69 3.062761252482577 1101 70-71 3.3357811822382075 1101 72-73 3.724918873888111 1101 74-75 3.8614216993228716 1101 76-77 4.0739695336802235 1101 78-79 4.27692499982577 1101 80-81 4.469248256712728 1101 82-83 4.704084053972519 1101 84-85 4.945107216964274 1101 86-87 5.1049489445966465 1101 88-89 4.995660084611323 1101 90-91 5.090799316392577 1101 92-93 5.267887676532581 1101 94-95 5.334773248306071 1101 96-97 5.55559454871409 1101 98-99 5.800042962069707 1101 100-101 5.117564547201965 1102 1 0.3781467893218746 1102 2 0.3123011859173772 1102 3 0.371214451848374 1102 4 0.2445306882659537 1102 5 0.2509978287679573 1102 6 0.1427379498587129 1102 7 0.15031900018216504 1102 8 0.267569879567489 1102 9 0.31111770252176996 1102 10-11 0.3492357312677754 1102 12-13 0.3819992290422789 1102 14-15 0.490770779763551 1102 16-17 0.49885428486051353 1102 18-19 0.48214278808934097 1102 20-21 0.4600963884876279 1102 22-23 0.5335745859091858 1102 24-25 0.45613723699447206 1102 26-27 0.5575260534209932 1102 28-29 0.6498575337331189 1102 30-31 0.6547871999573971 1102 32-33 0.5267227942126809 1102 34-35 0.7176922742333076 1102 36-37 0.7605719412027128 1102 38-39 1.0387918314378837 1102 40-41 1.1653528076030781 1102 42-43 1.4044662750530748 1102 44-45 1.472781935880711 1102 46-47 1.342647741338162 1102 48-49 1.477416973325873 1102 50-51 1.7735554148756094 1102 52-53 1.7519483966448206 1102 54-55 1.7543135362475013 1102 56-57 2.1598026443883214 1102 58-59 2.1234510056530738 1102 60-61 2.3244728937147983 1102 62-63 2.3945717693662054 1102 64-65 2.4396149796186783 1102 66-67 2.7097735822604996 1102 68-69 2.811442373743379 1102 70-71 2.9622067000466323 1102 72-73 3.1386680659263604 1102 74-75 3.4883453611956305 1102 76-77 3.5392975780325706 1102 78-79 3.667576288287126 1102 80-81 4.0381115495846025 1102 82-83 4.186662972824646 1102 84-85 4.169475521575361 1102 86-87 4.305407819343561 1102 88-89 4.507122150807156 1102 90-91 4.730333716552266 1102 92-93 4.743387127593866 1102 94-95 4.849163284711878 1102 96-97 4.668092231764955 1102 98-99 4.805971975500878 1102 100-101 3.7076638670882787 1103 1 0.45658908853160796 1103 2 0.24773426031856616 1103 3 0.21088528140771956 1103 4 0.2763837198597159 1103 5 0.27339989304194745 1103 6 0.2816319829710494 1103 7 0.24999272547165674 1103 8 0.23160726815763866 1103 9 0.27543063442609395 1103 10-11 0.33604641000222557 1103 12-13 0.43743927339858146 1103 14-15 0.609378427409311 1103 16-17 0.6580484525457422 1103 18-19 0.6652444068922847 1103 20-21 0.6471790750517741 1103 22-23 0.5475953072153672 1103 24-25 0.5643539273993952 1103 26-27 0.6433636882604432 1103 28-29 0.7396933525681604 1103 30-31 0.7521651050874425 1103 32-33 0.7779672286305654 1103 34-35 0.827290890119194 1103 36-37 0.6506705643840434 1103 38-39 0.8021862669220283 1103 40-41 0.9822472604840513 1103 42-43 1.060335964951804 1103 44-45 1.22999516600035 1103 46-47 1.2702690786043078 1103 48-49 1.3917089729173284 1103 50-51 1.5182909101296431 1103 52-53 1.5085061591657691 1103 54-55 1.5475853409271352 1103 56-57 1.7462241953548556 1103 58-59 1.8669086758024775 1103 60-61 2.046948024668019 1103 62-63 2.0280172280644457 1103 64-65 2.172977999797876 1103 66-67 2.2426634087074966 1103 68-69 2.5074445825793923 1103 70-71 2.8118624673197203 1103 72-73 3.1224817200796586 1103 74-75 3.3457107293854733 1103 76-77 3.475143406217004 1103 78-79 3.575762281707757 1103 80-81 3.8485458866588154 1103 82-83 4.320841343067315 1103 84-85 4.098557631238233 1103 86-87 4.177290840824718 1103 88-89 4.31867269083838 1103 90-91 4.70973951946079 1103 92-93 4.842757811356563 1103 94-95 4.986744979656812 1103 96-97 4.963068645032788 1103 98-99 4.716402159783801 1103 100-101 3.7812423497930254 1104 1 0.5265148136297455 1104 2 0.4164531359339918 1104 3 0.3762355846890557 1104 4 0.3372721945412991 1104 5 0.3152866073424505 1104 6 0.28128681021208024 1104 7 0.2856992784195853 1104 8 0.3493707997142721 1104 9 0.41274349587688874 1104 10-11 0.47128823489922667 1104 12-13 0.4111559367704132 1104 14-15 0.619630842834141 1104 16-17 0.6466836394567892 1104 18-19 0.5339453962829239 1104 20-21 0.4922474977064013 1104 22-23 0.6025632845971316 1104 24-25 0.6552135958452681 1104 26-27 0.6407427287429286 1104 28-29 0.648477528593709 1104 30-31 0.7362134167219665 1104 32-33 0.9292015350372864 1104 34-35 0.8474466257921733 1104 36-37 0.9961751598886366 1104 38-39 1.1404186999820425 1104 40-41 1.1903864341404642 1104 42-43 1.2547733490443633 1104 44-45 1.3983963257808156 1104 46-47 1.6062916215233969 1104 48-49 1.7252211684984857 1104 50-51 1.7556685699210917 1104 52-53 1.8079756913939207 1104 54-55 1.9181102545555007 1104 56-57 2.347566132903694 1104 58-59 2.5814186403124424 1104 60-61 2.593791890477405 1104 62-63 2.5611844077833474 1104 64-65 2.747762815962002 1104 66-67 3.020770669573377 1104 68-69 3.2652823576585455 1104 70-71 3.46564085385673 1104 72-73 3.5669293024582736 1104 74-75 3.7089085667212416 1104 76-77 3.8616623703221755 1104 78-79 4.054467475930192 1104 80-81 4.394807665679213 1104 82-83 4.511287274892556 1104 84-85 4.610837936621984 1104 86-87 4.929730584643771 1104 88-89 5.236734953728227 1104 90-91 5.049799799176242 1104 92-93 5.275174554118134 1104 94-95 5.048680739868431 1104 96-97 4.992238881099574 1104 98-99 4.864093738509862 1104 100-101 4.322670925704362 1105 1 0.41824124281388464 1105 2 0.37666443471464817 1105 3 0.3287552866045971 1105 4 0.25220912276967056 1105 5 0.21259812862690808 1105 6 0.26469893185523574 1105 7 0.2803384318310904 1105 8 0.27562547391481473 1105 9 0.35726462406162085 1105 10-11 0.3406094652644356 1105 12-13 0.38465505212549544 1105 14-15 0.5301163646053055 1105 16-17 0.5744845674993755 1105 18-19 0.5266969685415077 1105 20-21 0.5106669563143598 1105 22-23 0.5565974299072636 1105 24-25 0.6577325478567246 1105 26-27 0.6593468814698511 1105 28-29 0.8275657516645367 1105 30-31 0.7300179994583971 1105 32-33 0.8066661190025926 1105 34-35 0.9840812062401838 1105 36-37 1.1375219198917534 1105 38-39 1.0498139579756653 1105 40-41 1.157554060926266 1105 42-43 1.3020176692466308 1105 44-45 1.3416183056146878 1105 46-47 1.3712542642047438 1105 48-49 1.608534399564256 1105 50-51 1.5976827687501824 1105 52-53 1.5402860587937042 1105 54-55 1.6891269212947364 1105 56-57 1.979722576442441 1105 58-59 2.1916922778939565 1105 60-61 2.339541506695845 1105 62-63 2.4818196394383953 1105 64-65 2.656827511396923 1105 66-67 2.856308358059348 1105 68-69 2.8389239064088407 1105 70-71 3.0407695107881914 1105 72-73 3.333717719681683 1105 74-75 3.449678819816288 1105 76-77 3.5540552223332007 1105 78-79 3.6688122460265333 1105 80-81 3.9435095817083408 1105 82-83 4.092983398275486 1105 84-85 4.216892275155782 1105 86-87 4.303021952376898 1105 88-89 4.231396241405463 1105 90-91 4.457005095337557 1105 92-93 4.637794181962509 1105 94-95 4.598429860666371 1105 96-97 4.7560255048427 1105 98-99 4.794698582116158 1105 100-101 4.0788729953428735 1106 1 0.4057401928551272 1106 2 0.38730860678946044 1106 3 0.32504336683017243 1106 4 0.295884653153756 1106 5 0.2714350634909053 1106 6 0.30496433388960753 1106 7 0.1856505117041536 1106 8 0.2047903218004592 1106 9 0.3593824425158374 1106 10-11 0.345130025407677 1106 12-13 0.45740089968204245 1106 14-15 0.6700723099422774 1106 16-17 0.6206511967576773 1106 18-19 0.5058788682163993 1106 20-21 0.570774742900312 1106 22-23 0.5183059503398013 1106 24-25 0.553303993935657 1106 26-27 0.5626903173571876 1106 28-29 0.557527604310458 1106 30-31 0.6455009093427861 1106 32-33 0.6697497755855224 1106 34-35 0.8461376244831698 1106 36-37 0.7355363159164625 1106 38-39 0.8751149346782796 1106 40-41 1.062174889262252 1106 42-43 1.0242672018715453 1106 44-45 1.141774319158813 1106 46-47 1.3065239050890156 1106 48-49 1.3929786695893185 1106 50-51 1.5838172314030956 1106 52-53 1.5387898388747345 1106 54-55 1.5886205083990887 1106 56-57 1.3423237109946058 1106 58-59 2.0051500640438675 1106 60-61 2.1697267997456464 1106 62-63 1.9972743438732827 1106 64-65 2.278575680108201 1106 66-67 2.4658375313069065 1106 68-69 2.268471444180964 1106 70-71 2.4615020997179755 1106 72-73 2.8116419638376016 1106 74-75 3.183806541619216 1106 76-77 2.9966627553225607 1106 78-79 3.304140225602943 1106 80-81 3.5120851996234173 1106 82-83 3.619523716462332 1106 84-85 3.7718771809945615 1106 86-87 3.8416168298633515 1106 88-89 4.0715633718899795 1106 90-91 4.233823566533342 1106 92-93 4.288931901208812 1106 94-95 4.2647237059114005 1106 96-97 4.275374580901943 1106 98-99 4.164265705348495 1106 100-101 3.398349168049272 1107 1 0.45050023572481024 1107 2 0.343513939648858 1107 3 0.26556347590730667 1107 4 0.19819455357329474 1107 5 0.2300302735038855 1107 6 0.2429285086212687 1107 7 0.31446197306129875 1107 8 0.28449732510544834 1107 9 0.30812328019807467 1107 10-11 0.2923153111247885 1107 12-13 0.22903927379363154 1107 14-15 0.43011405761792787 1107 16-17 0.4103000441412519 1107 18-19 0.3582874604643109 1107 20-21 0.43591588253735836 1107 22-23 0.5174725227577923 1107 24-25 0.4772601225231696 1107 26-27 0.6510668524886114 1107 28-29 0.7418237103141863 1107 30-31 0.8110585832495474 1107 32-33 0.8057687922756216 1107 34-35 0.8156549096904371 1107 36-37 0.9796789850748837 1107 38-39 0.9675378796532144 1107 40-41 1.1133691995145298 1107 42-43 1.0591665891653932 1107 44-45 1.1778473268764387 1107 46-47 1.3517421760476722 1107 48-49 1.4053701265302507 1107 50-51 1.4345506656273912 1107 52-53 1.6574040422400387 1107 54-55 1.7248479941854882 1107 56-57 1.7515700896014081 1107 58-59 1.9370452972150929 1107 60-61 2.092209731759141 1107 62-63 2.129079098315085 1107 64-65 2.3332807535593325 1107 66-67 2.3593535814077065 1107 68-69 2.478190681861765 1107 70-71 2.7085191291074047 1107 72-73 2.8404540086389325 1107 74-75 3.033498752370029 1107 76-77 3.1429043873864977 1107 78-79 3.379887886889353 1107 80-81 3.424858273943432 1107 82-83 3.689513232419081 1107 84-85 3.8765320333652333 1107 86-87 4.132196046324733 1107 88-89 4.272382841691808 1107 90-91 4.265459930006507 1107 92-93 4.459575637621295 1107 94-95 4.604172431938576 1107 96-97 4.436991762525423 1107 98-99 4.298338711270905 1107 100-101 3.537737126120277 1108 1 0.3199033840613055 1108 2 0.30500512675334335 1108 3 0.3144409133053401 1108 4 0.23221744683184653 1108 5 0.2366340293984237 1108 6 -0.03579834296187556 1108 7 0.1746073173040088 1108 8 0.18812807375732632 1108 9 0.3111014665069405 1108 10-11 0.2901552833364178 1108 12-13 0.3689103373684617 1108 14-15 0.4918593538203311 1108 16-17 0.6565919889966949 1108 18-19 0.5005960336940731 1108 20-21 0.46742212911627234 1108 22-23 0.4675077046903482 1108 24-25 0.5145228342286714 1108 26-27 0.5700144662338502 1108 28-29 0.6395623782047792 1108 30-31 0.5959978586981833 1108 32-33 0.49544550692603906 1108 34-35 0.675518084118707 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34-35 0.5941433224888684 2311 36-37 0.8264370006767905 2311 38-39 0.8539348477087216 2311 40-41 0.9374835312375538 2311 42-43 0.9532270659191298 2311 44-45 0.9660508223826838 2311 46-47 0.9960550691815868 2311 48-49 1.072710763356504 2311 50-51 1.1226961237907531 2311 52-53 1.1922336335466 2311 54-55 1.6024656625951224 2311 56-57 1.7247248705887444 2311 58-59 1.7704971925488877 2311 60-61 1.8455752527870857 2311 62-63 1.9706886488665383 2311 64-65 2.347736473830391 2311 66-67 2.544749911973671 2311 68-69 2.669233677399337 2311 70-71 3.115783101367395 2311 72-73 3.361389552181681 2311 74-75 3.572245587952999 2311 76-77 3.7195597413774415 2311 78-79 4.115483755367524 2311 80-81 4.181963242308473 2311 82-83 4.445971959577243 2311 84-85 4.525810804226435 2311 86-87 4.602350573053233 2311 88-89 4.950635696576338 2311 90-91 5.2307096564018885 2311 92-93 5.4121562726788 2311 94-95 5.2939259851136775 2311 96-97 5.478443506777882 2311 98-99 5.456238514865163 2311 100-101 4.806876183593829 2312 1 -0.20058042782580543 2312 2 -0.12854262446646914 2312 3 -0.040834413320894214 2312 4 -0.0010024844644505038 2312 5 -0.019210515666422623 2312 6 0.07523704541558374 2312 7 0.09109269869551184 2312 8 0.05967696563401148 2312 9 0.11574115971006194 2312 10-11 -0.06412632923596817 2312 12-13 -0.015094073030510913 2312 14-15 0.07459653213403783 2312 16-17 0.07853081673476936 2312 18-19 0.05354892371935449 2312 20-21 0.1297909531349788 2312 22-23 0.10357147294214286 2312 24-25 0.14179417119868987 2312 26-27 0.1314232638098929 2312 28-29 0.2256765104924341 2312 30-31 0.2201567035373131 2312 32-33 0.17699847408749037 2312 34-35 0.3839660807189347 2312 36-37 0.3750695335610814 2312 38-39 0.3795348209519531 2312 40-41 0.4937533034342252 2312 42-43 0.6248671466838474 2312 44-45 0.7995523307227259 2312 46-47 0.805575388692283 2312 48-49 0.8385842518354636 2312 50-51 0.8219697648123727 2312 52-53 1.1081819028272903 2312 54-55 1.2923870864041334 2312 56-57 1.5867313518339188 2312 58-59 1.5383253792035205 2312 60-61 1.6938776158894981 2312 62-63 1.8159742591789403 2312 64-65 2.0221289388920916 2312 66-67 2.0279777087647552 2312 68-69 2.429433640608785 2312 70-71 2.780902310763093 2312 72-73 2.9814709421957346 2312 74-75 2.587406423025886 2312 76-77 2.802227609190286 2312 78-79 3.206403541051639 2312 80-81 3.5067140517370348 2312 82-83 3.773337370493568 2312 84-85 4.178201644977861 2312 86-87 4.2757918641254165 2312 88-89 4.390643898107143 2312 90-91 4.4687904981930515 2312 92-93 4.553282296325094 2312 94-95 4.635099123936946 2312 96-97 4.697591100159364 2312 98-99 4.6927672460345455 2312 100-101 4.323687794867446 2313 1 -0.33914968681103375 2313 2 -0.28331702345785104 2313 3 -0.32092337856667186 2313 4 -0.16020764251656772 2313 5 -0.12012042573645942 2313 6 -0.1619399742296892 2313 7 -0.055770783858044126 2313 8 -0.13080836089413594 2313 9 -0.1251428808599826 2313 10-11 -0.03382837680160122 2313 12-13 -0.034963154310482025 2313 14-15 -0.022063689093201333 2313 16-17 0.018786735641370456 2313 18-19 0.036777438947638075 2313 20-21 -0.009412492585816779 2313 22-23 -0.003954928778114208 2313 24-25 0.13088206169197747 2313 26-27 0.10117897437379497 2313 28-29 0.09761746518363168 2313 30-31 0.05969823293141019 2313 32-33 0.16530604624183098 2313 34-35 0.2716772405466159 2313 36-37 0.4246627598931525 2313 38-39 0.4111298440107589 2313 40-41 0.5774846090705736 2313 42-43 0.6344049814042592 2313 44-45 0.7545244619198712 2313 46-47 0.9266743906147852 2313 48-49 1.1003147051052977 2313 50-51 1.1120166422567976 2313 52-53 1.1678390105151522 2313 54-55 1.2459316190265497 2313 56-57 1.5581917275780341 2313 58-59 1.7179536839045966 2313 60-61 1.8365404593445618 2313 62-63 1.758695487433389 2313 64-65 1.9544602372829196 2313 66-67 2.1292138708685293 2313 68-69 2.259867521214243 2313 70-71 2.501440476688 2313 72-73 2.893083302948394 2313 74-75 2.949213076396429 2313 76-77 3.2151643808812977 2313 78-79 3.3658666342736936 2313 80-81 3.5847331313413093 2313 82-83 4.142528283088822 2313 84-85 4.3067311743486165 2313 86-87 4.441656661320668 2313 88-89 4.610925350017567 2313 90-91 4.798158106556013 2313 92-93 5.029703819651402 2313 94-95 4.855315311872232 2313 96-97 4.878044566741561 2313 98-99 4.961414595197763 2313 100-101 4.5335831839065435 2314 1 -0.37221334479781376 2314 2 -0.3383287343669288 2314 3 -0.3010972882048115 2314 4 -0.1468888277182927 2314 5 -0.11697683400871739 2314 6 -0.22307459418427555 2314 7 -0.2506789383151471 2314 8 -0.210508023534004 2314 9 -0.1829318719061348 2314 10-11 -0.3341160858843395 2314 12-13 -0.2832829970952986 2314 14-15 -0.2750519385141388 2314 16-17 -0.06399733162264454 2314 18-19 -0.28830432635127323 2314 20-21 -0.2430252619459452 2314 22-23 -0.11211932880970465 2314 24-25 -0.07780988551795076 2314 26-27 -0.1535422937839641 2314 28-29 -0.0980902555483496 2314 30-31 -0.04537190855437956 2314 32-33 0.0768193555841421 2314 34-35 0.05162257734666298 2314 36-37 0.17489127551492345 2314 38-39 0.15283420881488752 2314 40-41 0.16876371040309124 2314 42-43 0.3253704228745704 2314 44-45 0.37471789376613884 2314 46-47 0.4149582049190954 2314 48-49 0.5134380816717155 2314 50-51 0.5220283410868802 2314 52-53 0.6231372353216287 2314 54-55 0.8823746762648739 2314 56-57 1.0807483714162345 2314 58-59 1.282314922823545 2314 60-61 1.387230558085939 2314 62-63 1.547820991938277 2314 64-65 1.7424250769647038 2314 66-67 1.814085392895091 2314 68-69 2.090675559318786 2314 70-71 2.4753771717894786 2314 72-73 2.701262604652463 2314 74-75 2.9234528908426363 2314 76-77 3.056526668384656 2314 78-79 3.4773471014487534 2314 80-81 3.6595164499615755 2314 82-83 3.766547515909526 2314 84-85 4.044316854197373 2314 86-87 4.260737579055171 2314 88-89 4.396018756930239 2314 90-91 4.60971037385438 2314 92-93 4.705814823888076 2314 94-95 4.95588049467392 2314 96-97 4.955779230256841 2314 98-99 4.8519571699245 2314 100-101 4.415420613525704 2315 1 -0.2846048421933709 2315 2 -0.17175814699789527 2315 3 -0.22456284829998197 2315 4 -0.09107576260336714 2315 5 -0.22005504215757554 2315 6 -0.13172373204083243 2315 7 -0.07837906114726678 2315 8 -0.04220057968706925 2315 9 -0.1669671712523595 2315 10-11 -0.1865313948093288 2315 12-13 -0.13872201797555306 2315 14-15 -0.07226906187001703 2315 16-17 0.028036710045370228 2315 18-19 -0.09746609748715684 2315 20-21 -0.12264499162900222 2315 22-23 -0.0931518854923965 2315 24-25 -0.009879505483695539 2315 26-27 0.02716926498091965 2315 28-29 0.05437794392162232 2315 30-31 0.06864286365261307 2315 32-33 0.17533668286937854 2315 34-35 0.18696192099824316 2315 36-37 0.32751009441177814 2315 38-39 0.24221461657055698 2315 40-41 0.44643361948144644 2315 42-43 0.6169309300055019 2315 44-45 0.6697833281414276 2315 46-47 0.866499549712124 2315 48-49 0.9707874409759754 2315 50-51 1.048360559133485 2315 52-53 1.17792208619462 2315 54-55 1.3037977075209781 2315 56-57 1.562837540753094 2315 58-59 1.8609794832946065 2315 60-61 1.9010027695086436 2315 62-63 2.0385316556139763 2315 64-65 2.2297605833557235 2315 66-67 2.2998947542339714 2315 68-69 2.659391928319369 2315 70-71 2.996520808202572 2315 72-73 3.213635744930915 2315 74-75 3.500447277183504 2315 76-77 3.6814675200039133 2315 78-79 3.9658558097559613 2315 80-81 4.194154664374857 2315 82-83 4.557330661439888 2315 84-85 4.748143222710265 2315 86-87 4.790074816431268 2315 88-89 4.98321823852142 2315 90-91 5.084248800800424 2315 92-93 5.351791709497718 2315 94-95 5.432646639261144 2315 96-97 5.342853722886854 2315 98-99 5.379142898056795 2315 100-101 5.040664201805285 2316 1 -0.6551070492776851 2316 2 -0.6329405831952499 2316 3 -0.6502554192040257 2316 4 -0.3093210005955882 2316 5 -0.3726775536715934 2316 6 -0.3527838188367838 2316 7 -0.3527555473469235 2316 8 -0.3328234467585176 2316 9 -0.38203454295873485 2316 10-11 -0.3636573568257475 2316 12-13 -0.3301394262189561 2316 14-15 -0.4140158772036102 2316 16-17 -0.2751900253027131 2316 18-19 -0.37245430773980814 2316 20-21 -0.427797337889146 2316 22-23 -0.33588462095823246 2316 24-25 -0.36238669611056196 2316 26-27 -0.24399922781019967 2316 28-29 -0.2527816147143085 2316 30-31 -0.30393151562250154 2316 32-33 -0.13891755492647206 2316 34-35 0.009403276115072856 2316 36-37 0.031997222326033636 2316 38-39 -0.07758060162957037 2316 40-41 -0.005058843745395336 2316 42-43 0.24061464732289295 2316 44-45 0.17642971571578414 2316 46-47 0.3191526244747891 2316 48-49 0.5535111256803091 2316 50-51 0.5152322011787334 2316 52-53 0.5062362217602399 2316 54-55 0.8370213819534058 2316 56-57 0.9116227405659174 2316 58-59 1.1280782895834065 2316 60-61 1.1948185951197772 2316 62-63 1.3003006582822572 2316 64-65 1.6935063499211331 2316 66-67 1.8554273047824061 2316 68-69 2.157214798971637 2316 70-71 2.4109382604028795 2316 72-73 2.814280017753415 2316 74-75 3.0262176736185644 2316 76-77 3.37754877899399 2316 78-79 3.6103667799881833 2316 80-81 3.6331402399675987 2316 82-83 3.7819835654248024 2316 84-85 4.005026065158962 2316 86-87 4.452501095362056 2316 88-89 4.575190835120168 2316 90-91 4.8293335176955665 2316 92-93 4.950778933187529 2316 94-95 5.403908749934072 2316 96-97 5.735940483605997 2316 98-99 5.609088649725063 2316 100-101 5.294314145757355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4984.0 3 1677.0 4 1733.0 5 2169.0 6 2578.0 7 3249.0 8 4189.0 9 5464.0 10 7078.0 11 8875.0 12 11632.0 13 14522.0 14 17699.0 15 21733.0 16 26166.0 17 32678.0 18 33181.0 19 30491.0 20 32352.0 21 34939.0 22 38813.0 23 43741.0 24 49183.0 25 53731.0 26 59735.0 27 65357.0 28 71774.0 29 78929.0 30 87294.0 31 97991.0 32 111342.0 33 130859.0 34 158809.0 35 199348.0 36 249433.0 37 284784.0 38 171295.0 39 13615.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85011487319596 18.446018468416526 14.831149707716463 22.872716950671055 2 15.471664594851497 19.504567347102913 40.78149781409874 24.242270243946844 3 19.537962022507866 23.71193256303431 29.54084379301109 27.20926162144674 4 12.892412008798567 16.39447000209048 36.74109149440852 33.972026494702426 5 13.832191727835944 37.08115897823545 34.09782221898049 14.988827074948114 6 32.61398488011154 35.77959548861146 17.049172791273573 14.55724684000343 7 28.809208028740617 32.78844364815636 20.090280684185103 18.312067638917924 8 26.67051154568556 34.70343608441056 19.252414097889737 19.373638272014144 9 26.281419805056338 17.128481176291206 20.084544825772692 36.505554192879764 10-11 25.26654588659445 26.494167268246883 26.681612813912732 21.55767403124593 12-13 26.316238387041746 23.807216275244457 27.200661665725345 22.675883671988455 14-15 22.894805803726296 25.26473877884529 25.799563478753505 26.04089193867491 16-17 22.24037323570823 28.429149471938825 26.692677145102145 22.637800147250804 18-19 22.192695214105793 26.706836360422464 29.232643952450392 21.867824473021344 20-21 22.726881992260793 25.82689522903711 29.66091975486109 21.785303023841003 22-23 22.515052487610006 25.697231226095546 29.583037807429797 22.20467847886465 24-25 22.345972396658745 25.94352079952494 29.603965800692922 22.106541003123393 26-27 21.83311967658239 26.248532928368896 29.480874866386266 22.437472528662454 28-29 22.004749886312965 26.67069119507723 29.32659269017775 21.997966228432055 30-31 22.106242696494142 26.211343350075044 29.18275426807601 22.499659685354803 32-33 21.843138788731753 26.19529503430534 29.232382011800535 22.729184165162376 34-35 22.597774177381673 26.30772161218784 29.016901826744345 22.07760238368614 36-37 22.660191810541512 25.85758660609895 29.055975788233667 22.426245795125872 38-39 22.094728590643882 26.330953975915968 28.973419363733257 22.600898069706897 40-41 22.640157601564702 26.132507178519738 29.032967173778612 22.194368046136944 42-43 22.282439877711838 26.132599321573768 28.822267262112277 22.762693538602115 44-45 22.324292725862012 26.455154569025396 28.866832567749324 22.35372013736327 46-47 22.83256323561676 26.693900132322916 28.17786550697971 22.295671125080617 48-49 22.492329092535183 26.75684107081626 28.145558120380223 22.605271716268334 50-51 22.608608626742633 26.982601896910424 28.09182458604639 22.316964890300554 52-53 22.900331480972152 26.705392834223062 27.9754034712166 22.418872213588177 54-55 22.581325719744193 26.976856800944983 27.96845255965624 22.473364919654585 56-57 22.551639030265648 27.15975060742054 27.81959554783611 22.469014814477706 58-59 22.588065634437733 26.696988916230314 27.864216586487007 22.85072886284495 60-61 22.24441446858861 26.888600421596227 28.215775793166003 22.65120931664916 62-63 22.372470524663097 26.859609935180657 28.395228098204804 22.372691441951442 64-65 22.18020794856251 26.941299510615696 28.14474481851778 22.733747722304013 66-67 22.15005703994917 26.92426985772266 28.00506864676145 22.92060445556672 68-69 22.599224894739134 26.60953194343406 28.190604405812646 22.600638756014156 70-71 22.514951485139985 26.690585507868846 27.789933097503194 23.004529909487974 72-73 22.51658145709147 26.74894300874601 27.753407812268367 22.981067721894153 74-75 22.473457077324753 27.13689913623106 27.580558858370434 22.80908492807375 76-77 22.609068140400936 26.754166693361014 27.704713809697495 22.932051356540555 78-79 22.732931247364167 26.973081772232828 27.607802037794254 22.686184942608747 80-81 22.890855848242936 26.948388857039024 27.265436783170898 22.895318511547142 82-83 22.855963737917776 26.966311496977386 27.409866734594367 22.767858030510467 84-85 22.79182793418518 27.019555343477723 27.382407260131135 22.806209462205963 86-87 22.983765681503392 26.886894360954578 27.210078575957127 22.919261381584906 88-89 22.896027599354206 26.977621924287813 27.043155807802332 23.083194668555645 90-91 22.880970592953073 26.998924892257236 27.011894235557744 23.10821027923195 92-93 23.0408860861289 26.566036251870166 27.16426899308314 23.228808668917793 94-95 22.920239185005133 26.398554620118258 27.322089967694602 23.359116227182007 96-97 23.11342942672505 26.09276056139492 27.343267898533895 23.45054211334613 98-99 22.835483321472623 26.278697409210345 26.96431337103573 23.921505898281296 100-101 23.650952658953404 25.191195202207833 27.202128224180022 23.95572391465874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 626.0 1 437.0 2 357.0 3 480.0 4 420.0 5 396.0 6 714.0 7 1639.0 8 2863.0 9 3416.5 10 2857.5 11 2243.0 12 2232.0 13 2335.5 14 2493.0 15 2693.0 16 2833.0 17 3058.0 18 3365.5 19 3682.0 20 4106.0 21 4877.5 22 5871.0 23 7171.0 24 8944.5 25 11211.0 26 14317.5 27 17860.0 28 20855.5 29 23315.5 30 26304.5 31 31289.5 32 37377.0 33 40501.0 34 41357.0 35 43990.5 36 48362.0 37 52720.5 38 55993.5 39 58401.5 40 61631.0 41 65578.5 42 68121.5 43 70114.0 44 72837.0 45 76350.5 46 78496.5 47 80383.0 48 82139.0 49 81163.0 50 79463.5 51 77857.0 52 77197.0 53 77924.5 54 78859.5 55 75778.0 56 70223.5 57 65205.0 58 60867.5 59 54557.5 60 44930.5 61 37676.5 62 32114.5 63 24814.5 64 18146.5 65 13957.0 66 10673.0 67 8199.0 68 6407.0 69 4954.5 70 3824.0 71 2737.5 72 1940.5 73 1447.5 74 1006.5 75 690.5 76 496.0 77 341.5 78 224.5 79 147.0 80 92.0 81 63.5 82 45.0 83 22.5 84 14.0 85 9.5 86 6.0 87 7.0 88 7.0 89 3.5 90 5.0 91 6.0 92 2.5 93 1.5 94 1.5 95 0.5 96 1.5 97 1.5 98 1.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3871569741935113 2 0.022620606046682392 3 0.04086730584605706 4 0.03454944126661256 5 0.055579535950637596 6 0.05465173765575414 7 0.03101497157181844 8 0.02973372630745557 9 0.03172186551077726 10-11 0.027944401024466044 12-13 0.02867338539901733 14-15 0.023504223470380923 16-17 0.02725959752109968 18-19 0.02310659562971658 20-21 0.03479243605812966 22-23 0.022443882561942685 24-25 0.04327516332563555 26-27 0.03700147961737599 28-29 0.027922310588873577 30-31 0.03583068653097543 32-33 0.03218576465821899 34-35 0.03132423767011292 36-37 0.031876498559924506 38-39 0.015330762301169512 40-41 0.01093476561826932 42-43 0.010780132569122077 44-45 0.010029057758978326 46-47 0.01760607716719323 48-49 0.01444714487747098 50-51 0.009565158611536596 52-53 0.004219273198160485 54-55 0.004484358425270044 56-57 0.007245662874327954 58-59 0.0030263896761674684 60-61 0.005301704542191185 62-63 0.005853965432002768 64-65 0.007554928972622439 66-67 0.004285544504937875 68-69 0.005191252364228869 70-71 0.015617937963871535 72-73 0.012260191753817117 74-75 0.008924535979355164 76-77 0.006516678499776665 78-79 0.006295774143852033 80-81 0.008858264672577772 82-83 0.010272052550495422 84-85 0.0045506297320474344 86-87 3.534469694794124E-4 88-89 0.020831280763692867 90-91 0.01740726324686106 92-93 0.011354483894526122 94-95 0.021582355573836618 96-97 0.016855002357049478 98-99 0.015397033607946902 100-101 0.10528301603367997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2263423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.69714630741353 #Duplication Level Percentage of deduplicated Percentage of total 1 76.89381958671429 42.058725000703724 2 13.149628030837334 14.384942565815518 3 4.2518442530484615 6.976912415559814 4 1.861529525976217 4.072814113515653 5 0.973168547065948 2.661477120031961 6 0.6202594407411425 2.0355852827263656 7 0.41196806915912954 1.57734344269457 8 0.28433281897523244 1.2441755039590094 9 0.2141636837995279 1.054272811186569 >10 1.2028361637630212 11.982038960290605 >50 0.08623437002451234 3.2390707008997834 >100 0.044206901114508826 4.435257892470816 >500 0.003410291470083993 1.2852138271668367 >1k 0.0024359224786313326 2.2077982086629615 >5k 1.6239483190875548E-4 0.7843721543158771 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9809 0.4333701654529445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7852 0.34690820054404325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4963 0.21926966369079046 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2737 0.12092304443314396 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2484 0.10974528402335755 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2438 0.10771296394885092 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2434 0.10753624046411121 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3254261355477963E-4 2 0.0 0.0 0.0 0.0 1.3254261355477963E-4 3 0.0 0.0 0.0 0.0 1.3254261355477963E-4 4 0.0 0.0 0.0 0.0 1.3254261355477963E-4 5 0.0 0.0 0.0 0.0 1.3254261355477963E-4 6 0.0 0.0 0.0 0.0 1.3254261355477963E-4 7 0.0 0.0 0.0 0.0 1.3254261355477963E-4 8 0.0 0.0 0.0 0.0 1.3254261355477963E-4 9 0.0 0.0 0.0 0.0 1.3254261355477963E-4 10-11 0.0 0.0 0.0 0.0 1.3254261355477963E-4 12-13 0.0 0.0 0.0 0.0 2.4299479151709602E-4 14-15 0.0 0.0 0.0 0.0 2.6508522710955927E-4 16-17 0.0 0.0 0.0 0.0 3.092660982944858E-4 18-19 0.0 0.0 0.0 0.0 3.092660982944858E-4 20-21 0.0 0.0 0.0 2.2090435592463273E-5 3.534469694794124E-4 22-23 0.0 0.0 0.0 8.83617423698531E-5 3.534469694794124E-4 24-25 0.0 2.2090435592463273E-5 0.0 3.092660982944858E-4 3.534469694794124E-4 26-27 0.0 4.418087118492655E-5 0.0 6.848035033663615E-4 3.534469694794124E-4 28-29 0.0 4.418087118492655E-5 0.0 0.003225203596499638 3.534469694794124E-4 30-31 0.0 4.418087118492655E-5 0.0 0.011023127360639174 3.534469694794124E-4 32-33 0.0 4.418087118492655E-5 0.0 0.028430390607500234 3.534469694794124E-4 34-35 0.0 4.418087118492655E-5 0.0 0.053746029796463146 3.9762784066433893E-4 36-37 0.0 4.418087118492655E-5 0.0 0.09794899141698216 3.9762784066433893E-4 38-39 0.0 4.418087118492655E-5 0.0 0.1613927224385367 3.9762784066433893E-4 40-41 0.0 4.418087118492655E-5 0.0 0.22742103442440942 3.9762784066433893E-4 42-43 0.0 4.418087118492655E-5 0.0 0.29477477254582996 3.9762784066433893E-4 44-45 0.0 4.418087118492655E-5 0.0 0.379226507815817 3.9762784066433893E-4 46-47 0.0 6.627130677738982E-5 0.0 0.47476764175322067 4.197182762568022E-4 48-49 0.0 8.83617423698531E-5 0.0 0.5701541426414771 5.080800186266553E-4 50-51 0.0 8.83617423698531E-5 0.0 0.679391346646208 5.743513254040451E-4 52-53 0.0 8.83617423698531E-5 0.0 0.7793947485732893 6.185321965889716E-4 54-55 0.0 8.83617423698531E-5 0.0 0.8889853995474994 6.627130677738983E-4 56-57 0.0 8.83617423698531E-5 0.0 1.0139951745652491 7.068939389588248E-4 58-59 0.0 8.83617423698531E-5 0.0 1.1513755935147785 7.952556813286779E-4 60-61 0.0 8.83617423698531E-5 0.0 1.2966202075352244 7.952556813286779E-4 62-63 0.0 8.83617423698531E-5 0.0 1.4360992178660372 8.394365525136044E-4 64-65 0.0 8.83617423698531E-5 0.0 1.5742086211901178 8.394365525136044E-4 66-67 0.0 8.83617423698531E-5 0.0 1.7353583488371376 8.394365525136044E-4 68-69 0.0 8.83617423698531E-5 0.0 1.915196584995381 8.615269881060677E-4 70-71 0.0 8.83617423698531E-5 0.0 2.1043790754092364 9.277982948834575E-4 72-73 0.0 8.83617423698531E-5 0.0 2.294312640633236 9.498887304759207E-4 74-75 0.0 8.83617423698531E-5 0.0 2.4874272285825496 0.0010161600372533106 76-77 0.0 8.83617423698531E-5 0.0 2.6909243212603213 0.001060340908438237 78-79 0.0 8.83617423698531E-5 0.0 2.9173291956474774 0.0011045217796231636 80-81 0.0 8.83617423698531E-5 0.0 3.1532771382105773 0.0011487026508080903 82-83 0.0 8.83617423698531E-5 0.0 3.3981717071886255 0.0011487026508080903 84-85 0.0 8.83617423698531E-5 0.0 3.641718759595533 0.0011487026508080903 86-87 0.0 8.83617423698531E-5 0.0 3.893196278380135 0.0011707930864005536 88-89 0.0 1.3254261355477963E-4 0.0 4.159827835981167 0.0011928835219930168 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 43.97884 1 GTATCAA 9775 0.0 38.374725 1 TATCAAC 12165 0.0 30.785383 2 ATCAACG 12180 0.0 30.552372 3 TCAACGC 12295 0.0 30.266605 4 CAACGCA 12385 0.0 30.008287 5 AACGCAG 12835 0.0 29.096579 6 ACGCAGA 14160 0.0 26.032614 7 CGCAGAG 14395 0.0 25.442629 8 GTACATG 8400 0.0 24.941624 1 TACATGG 8415 0.0 24.285374 2 ACATGGG 8345 0.0 23.862621 3 GCAGAGT 15700 0.0 22.782187 9 GAGTACT 9335 0.0 21.753857 12-13 AGAGTAC 13035 0.0 21.154175 10-11 TAAGGTG 1275 0.0 20.874126 5 GTACTTT 9670 0.0 20.73097 14-15 AGTACTT 9670 0.0 19.993204 12-13 CAGAGTA 14960 0.0 19.92448 10-11 GTGTAAG 970 0.0 19.68012 1 >>END_MODULE