FastQCFastQC Report
Thu 26 May 2016
SRR938231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263423
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94530.41764177531111063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79360.3506193937235771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49760.2198440150161945No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26400.11663749992820609No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25880.11434009462658991No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25770.11385410504355571No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23880.10550392038960459No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23350.1031623342168035No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23160.10232289766428988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58800.043.515981
GTATCAA95200.037.5186161
TATCAAC119450.029.7425562
ATCAACG120350.029.3970283
TCAACGC122050.029.0258854
CAACGCA123400.028.7083425
AACGCAG127400.027.8471376
ACGCAGA142450.024.7383567
CGCAGAG144200.024.4381338
GTGTAAG11350.024.3038981
TAAGGTG13650.023.3404435
TGGTATC31050.021.9037382
GAGTACT94050.021.81495512-13
GCAGAGT161900.021.5610499
TAAGGTA9100.021.4244384
GTACTTT95750.021.27883514-15
AGTACTT95800.020.94549412-13
AGAGTAC134650.020.79250510-11
TACATGG83150.020.6485142
GTACATG83650.020.6388021
Produced by FastQC (version 0.11.5)