##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938231_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2263423 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52778954707096 34.0 31.0 34.0 31.0 34.0 2 32.748350617626485 34.0 31.0 34.0 31.0 34.0 3 32.80974126356408 34.0 31.0 34.0 31.0 34.0 4 36.25897987252051 37.0 35.0 37.0 35.0 37.0 5 36.1850789710982 37.0 37.0 37.0 35.0 37.0 6 36.240117733185535 37.0 37.0 37.0 35.0 37.0 7 36.21443097467862 37.0 37.0 37.0 35.0 37.0 8 36.207175150203916 37.0 37.0 37.0 35.0 37.0 9 37.905279746649214 39.0 38.0 39.0 35.0 39.0 10-11 37.87976728167912 39.0 38.0 39.0 35.0 39.0 12-13 37.803810423416216 39.0 38.0 39.0 35.0 39.0 14-15 39.06661017405938 40.0 38.0 41.0 36.0 41.0 16-17 38.984950890752636 40.0 38.0 41.0 36.0 41.0 18-19 39.22733443991689 40.5 39.0 41.0 36.0 41.0 20-21 39.200318499900376 40.5 39.0 41.0 36.0 41.0 22-23 39.09759156816909 40.0 39.0 41.0 35.5 41.0 24-25 38.99728795722231 40.0 38.5 41.0 35.0 41.0 26-27 38.84525274330075 40.0 38.0 41.0 35.0 41.0 28-29 38.725011188805624 40.0 38.0 41.0 35.0 41.0 30-31 38.48506355197416 40.0 38.0 41.0 34.0 41.0 32-33 38.26163492197438 40.0 38.0 41.0 34.0 41.0 34-35 38.17529091999154 40.0 38.0 41.0 34.0 41.0 36-37 38.123717263631235 40.0 38.0 41.0 34.0 41.0 38-39 37.93615400214631 40.0 38.0 41.0 33.0 41.0 40-41 37.7251200946531 40.0 37.0 41.0 33.0 41.0 42-43 37.47140348931684 40.0 37.0 41.0 32.5 41.0 44-45 37.20236584147108 40.0 37.0 41.0 32.0 41.0 46-47 36.83523892794233 40.0 36.0 41.0 31.0 41.0 48-49 36.422240562192755 39.0 36.0 41.0 30.0 41.0 50-51 36.04370924038503 39.0 35.0 40.5 29.5 41.0 52-53 35.524196316817495 38.5 35.0 40.0 28.0 41.0 54-55 35.16226882911413 38.0 34.5 40.0 27.0 41.0 56-57 34.79510325732309 38.0 34.0 40.0 26.0 41.0 58-59 34.296291722757964 37.5 33.5 40.0 25.5 41.0 60-61 33.83055465107494 37.0 33.0 40.0 23.5 41.0 62-63 33.29482535964334 36.5 32.5 39.0 22.5 40.5 64-65 32.74502821611338 36.0 32.0 39.0 20.5 40.0 66-67 32.19669566846321 35.0 31.0 39.0 18.5 40.0 68-69 31.601283321765308 35.0 31.0 38.0 14.5 40.0 70-71 30.998761389276332 35.0 30.0 37.5 9.5 39.5 72-73 30.344499459447043 34.0 29.0 37.0 7.0 39.0 74-75 29.707777998191233 34.0 29.0 36.0 4.0 39.0 76-77 28.413041221194625 32.5 27.0 35.0 2.0 37.0 78-79 29.07278467171183 34.0 29.0 35.0 2.0 37.0 80-81 28.815509959914696 34.0 29.0 35.0 2.0 37.0 82-83 28.280352368956223 34.0 27.5 35.0 2.0 36.5 84-85 27.64726036626826 33.0 26.0 35.0 2.0 36.0 86-87 26.969660333044242 33.0 25.5 35.0 2.0 35.5 88-89 26.19827182104273 32.0 24.0 35.0 2.0 35.0 90-91 25.576060462405835 32.0 21.5 34.0 2.0 35.0 92-93 24.67814014437425 31.0 17.5 34.0 2.0 35.0 94-95 23.81655969741405 31.0 4.5 34.0 2.0 35.0 96-97 22.807110071780663 31.0 2.0 34.0 2.0 35.0 98-99 21.436526888699106 30.0 2.0 34.0 2.0 35.0 100-101 17.52405560074277 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19633965181281354 1101 2 0.08186972341393783 1101 3 0.20792458205655606 1101 4 -0.030023920469581356 1101 5 -0.025032150065193548 1101 6 0.03020857502783514 1101 7 0.11308380451156097 1101 8 0.17013258895117644 1101 9 0.15775989115586952 1101 10-11 0.18519884525407093 1101 12-13 0.2361794672792783 1101 14-15 0.3634202940712754 1101 16-17 0.4006619581186186 1101 18-19 0.27128868442243714 1101 20-21 0.20087090112877348 1101 22-23 0.2832629202777355 1101 24-25 0.3330984100734682 1101 26-27 0.39498388726008926 1101 28-29 0.43621260573060283 1101 30-31 0.48229564014930304 1101 32-33 0.4059023441870906 1101 34-35 0.3423651660570286 1101 36-37 0.40343401848134874 1101 38-39 0.4846100925528205 1101 40-41 0.6107253282559313 1101 42-43 0.5803642521809778 1101 44-45 0.6470662543251606 1101 46-47 0.7405208581405915 1101 48-49 0.9402932805053581 1101 50-51 0.8598237595117055 1101 52-53 0.8928889409009528 1101 54-55 1.0422112524679505 1101 56-57 1.1245137291418033 1101 58-59 1.3553008988330433 1101 60-61 1.131877780675424 1101 62-63 1.0098598403689962 1101 64-65 1.2268498385649949 1101 66-67 1.294938922891184 1101 68-69 1.5282731443876685 1101 70-71 1.7548653314262381 1101 72-73 1.9296005363435462 1101 74-75 1.7000410571478 1101 76-77 1.943199228095164 1101 78-79 1.9433752918749043 1101 80-81 2.062215716989371 1101 82-83 2.1251561728483566 1101 84-85 2.25759844517048 1101 86-87 2.416203198406542 1101 88-89 2.5862904569231695 1101 90-91 3.070704439084075 1101 92-93 3.3824424419497383 1101 94-95 3.5513990647889138 1101 96-97 3.8605932517242714 1101 98-99 4.085241960913606 1101 100-101 4.317572253646766 1102 1 0.14944341311239384 1102 2 0.07474599595369114 1102 3 0.08585166113224574 1102 4 0.099915933907198 1102 5 -0.1126312441974946 1102 6 -0.007558289985517774 1102 7 0.03918085022318252 1102 8 0.1211499078489311 1102 9 0.11390420262987533 1102 10-11 0.15559120573808372 1102 12-13 0.24839454194444954 1102 14-15 0.27551999655123893 1102 16-17 0.23988291664641537 1102 18-19 0.29931545171994856 1102 20-21 0.22765691245924558 1102 22-23 0.32529118943355684 1102 24-25 0.31209942477836705 1102 26-27 0.3225851676418259 1102 28-29 0.31571253681622125 1102 30-31 0.2880013353290991 1102 32-33 0.2416921386796389 1102 34-35 0.2494575849994547 1102 36-37 0.2036277510745279 1102 38-39 0.32197867995051155 1102 40-41 0.4696659002928527 1102 42-43 0.5682590840774111 1102 44-45 0.5353762359796761 1102 46-47 0.5952852778814659 1102 48-49 0.6523802694695533 1102 50-51 0.556776317898823 1102 52-53 0.6959859889889373 1102 54-55 0.7472829353096984 1102 56-57 0.8788027584470584 1102 58-59 0.9655749048024589 1102 60-61 0.8987828950733032 1102 62-63 1.3160446687484182 1102 64-65 1.11070117835569 1102 66-67 1.2030374324393946 1102 68-69 1.5027442264860866 1102 70-71 1.523661729817917 1102 72-73 1.85146172376216 1102 74-75 1.7539478492544873 1102 76-77 1.8267357004391087 1102 78-79 1.902894625924315 1102 80-81 2.049387541937328 1102 82-83 1.845915383612308 1102 84-85 2.0867469128947853 1102 86-87 2.049859379789204 1102 88-89 2.365831261367912 1102 90-91 2.40620966469551 1102 92-93 2.5496055694270936 1102 94-95 2.906186951065923 1102 96-97 2.9192093914731387 1102 98-99 3.259716548140208 1102 100-101 3.25993695569899 1103 1 0.18842521602989848 1103 2 0.18750438094438238 1103 3 0.13503305617830108 1103 4 0.06839793155694451 1103 5 -0.04168922277582254 1103 6 0.0641289891168455 1103 7 0.043843761419672944 1103 8 0.006273599298779686 1103 9 0.18276197177284104 1103 10-11 0.25490240357144245 1103 12-13 0.1965292652282571 1103 14-15 0.3195712913803419 1103 16-17 0.30143906855776237 1103 18-19 0.254266925792507 1103 20-21 0.27118910837671706 1103 22-23 0.24293825155818638 1103 24-25 0.3662787602644215 1103 26-27 0.31134597471997694 1103 28-29 0.3351798683784395 1103 30-31 0.4616267333517001 1103 32-33 0.37317568408592194 1103 34-35 0.5239767310508725 1103 36-37 0.4483935504812351 1103 38-39 0.5108796156754067 1103 40-41 0.5333598979381051 1103 42-43 0.6676623167448383 1103 44-45 0.6830343422843939 1103 46-47 0.7386202723313247 1103 48-49 0.7642805979890142 1103 50-51 0.9007494953565711 1103 52-53 0.9951168771138654 1103 54-55 1.0796586012995704 1103 56-57 1.205704315968262 1103 58-59 1.18107783588745 1103 60-61 1.1791473754637067 1103 62-63 1.1493322652587281 1103 64-65 1.4214539896270324 1103 66-67 1.3855018222540565 1103 68-69 1.464931939386787 1103 70-71 1.5634190945688005 1103 72-73 1.5218891918950952 1103 74-75 1.659895263692956 1103 76-77 1.656930028624103 1103 78-79 1.6086704851873392 1103 80-81 1.815660871925985 1103 82-83 2.0520175737046458 1103 84-85 2.202562369583976 1103 86-87 2.411288029784096 1103 88-89 2.477187237515178 1103 90-91 2.746149864500893 1103 92-93 2.830847186681158 1103 94-95 3.086677081452347 1103 96-97 3.4364493678134522 1103 98-99 3.9913760821072337 1103 100-101 3.9811564284740797 1104 1 0.26747605370142935 1104 2 0.2536504643318409 1104 3 0.23540615655140584 1104 4 0.10765191908679128 1104 5 0.11508196847812258 1104 6 0.15087404137914007 1104 7 0.16740305739276096 1104 8 0.17082686730032748 1104 9 0.1951003289387856 1104 10-11 0.2573766646663955 1104 12-13 0.2592251899931455 1104 14-15 0.4135688939986295 1104 16-17 0.4118174720396155 1104 18-19 0.3277832320674321 1104 20-21 0.37048825077931014 1104 22-23 0.4113888338018796 1104 24-25 0.41182509029351166 1104 26-27 0.38805826694950696 1104 28-29 0.48864916839946915 1104 30-31 0.38058801438194223 1104 32-33 0.4666641568847467 1104 34-35 0.4268121683690751 1104 36-37 0.542596687787821 1104 38-39 0.5655267392880461 1104 40-41 0.6944849728562872 1104 42-43 0.6764736359009831 1104 44-45 0.6018850111350602 1104 46-47 0.7203206247213316 1104 48-49 0.6550185145890026 1104 50-51 0.8552439584717249 1104 52-53 0.9234535209677475 1104 54-55 0.9244477478128559 1104 56-57 0.9666000078984425 1104 58-59 1.168472754316852 1104 60-61 1.2798562347932503 1104 62-63 1.3495983620765486 1104 64-65 1.5792293198161573 1104 66-67 1.6132766189943695 1104 68-69 1.7100602965841105 1104 70-71 1.6046946961892274 1104 72-73 1.838111370186244 1104 74-75 1.998767050840609 1104 76-77 2.0114483400389673 1104 78-79 2.0910855020851145 1104 80-81 2.3431044597143966 1104 82-83 2.4754990489102617 1104 84-85 2.58785597039482 1104 86-87 2.6087001754721086 1104 88-89 2.7155117689431556 1104 90-91 3.0503325738560143 1104 92-93 3.3028811328530345 1104 94-95 3.688664648957154 1104 96-97 3.7478807051068586 1104 98-99 4.208491316463846 1104 100-101 3.841232805791833 1105 1 0.13308017885123036 1105 2 0.1265235791092607 1105 3 0.10153714979694684 1105 4 0.08206664313902223 1105 5 -0.04534598001168888 1105 6 -0.03023408462990318 1105 7 0.093298745250749 1105 8 0.05437885815052823 1105 9 0.12415916060757581 1105 10-11 0.15456783844046385 1105 12-13 0.17927513360519498 1105 14-15 0.28814719390317123 1105 16-17 0.26937899683168354 1105 18-19 0.20127816247391195 1105 20-21 0.18015969119023367 1105 22-23 0.14667861851167174 1105 24-25 0.18553088889056113 1105 26-27 0.20738230340742803 1105 28-29 0.30440474998395217 1105 30-31 0.2672558567046863 1105 32-33 0.20020533460184708 1105 34-35 0.2415657813343799 1105 36-37 0.3354753566935571 1105 38-39 0.2504574408934701 1105 40-41 0.2774784303060045 1105 42-43 0.16908224257435478 1105 44-45 0.3566652868191582 1105 46-47 0.3719103488098412 1105 48-49 0.36640679992605385 1105 50-51 0.2951020137236924 1105 52-53 0.4141660798291724 1105 54-55 0.5006018381042097 1105 56-57 0.45902307378694474 1105 58-59 0.584080967653442 1105 60-61 0.7072638260481128 1105 62-63 0.8164385710627258 1105 64-65 0.832553451100658 1105 66-67 0.9197579432379044 1105 68-69 1.2932956585773212 1105 70-71 1.2498281089640102 1105 72-73 1.3732255080069997 1105 74-75 1.1185893864676792 1105 76-77 1.0929255398100253 1105 78-79 1.3405176311692486 1105 80-81 1.4979176827973149 1105 82-83 1.6833208146102265 1105 84-85 1.7080462428325554 1105 86-87 1.8508065883131728 1105 88-89 1.8499560247020561 1105 90-91 2.237581446701796 1105 92-93 2.4058011142167075 1105 94-95 2.6089821389943175 1105 96-97 2.823631298619656 1105 98-99 2.8036066105883215 1105 100-101 3.028649197533987 1106 1 0.1733049595303342 1106 2 0.15674586742724728 1106 3 0.10408252522777417 1106 4 0.12096009906164085 1106 5 0.08072710078992174 1106 6 0.12253801304311196 1106 7 0.13792486124789605 1106 8 0.11504031151376637 1106 9 0.1806884811936058 1106 10-11 0.1740176646407363 1106 12-13 0.11107704184499312 1106 14-15 0.08272522982163366 1106 16-17 0.09774057462939112 1106 18-19 0.03660102421855527 1106 20-21 -0.021519141228090177 1106 22-23 0.0028777586241375275 1106 24-25 0.04302038815547604 1106 26-27 -0.04967433744976546 1106 28-29 0.03071687713384108 1106 30-31 0.12841917887977417 1106 32-33 0.28666364355089513 1106 34-35 0.19812835635192272 1106 36-37 0.2277305395883431 1106 38-39 0.20447337823468814 1106 40-41 0.27836372340170357 1106 42-43 0.20593258202659115 1106 44-45 0.32740540332212476 1106 46-47 0.5637794681801651 1106 48-49 0.6427305856344034 1106 50-51 0.686889706784136 1106 52-53 0.7137823112965336 1106 54-55 0.7914493648144756 1106 56-57 1.0008008754326383 1106 58-59 1.0447015472123127 1106 60-61 1.0241453957490805 1106 62-63 1.0936821894937125 1106 64-65 1.0540182112717176 1106 66-67 1.3627440351284505 1106 68-69 1.5003249201820665 1106 70-71 1.156554248048785 1106 72-73 1.5068634556049894 1106 74-75 1.6753988108056994 1106 76-77 1.7476396595636174 1106 78-79 1.8003909613905726 1106 80-81 1.4843161842594554 1106 82-83 2.1494165561611 1106 84-85 2.1522344514399663 1106 86-87 2.2364048031767325 1106 88-89 2.348965735730456 1106 90-91 2.345262118785559 1106 92-93 2.214138544110444 1106 94-95 2.7076130845722552 1106 96-97 3.1426857665785874 1106 98-99 3.2549929462988096 1106 100-101 3.273004320896682 1107 1 0.14105710166811036 1107 2 0.1613223138751465 1107 3 0.12327559498795182 1107 4 0.07090013895126646 1107 5 -4.376153779404035E-4 1107 6 0.030496102003091607 1107 7 0.10924139856947335 1107 8 0.1025752508445521 1107 9 0.14047481495599357 1107 10-11 0.1816993805770153 1107 12-13 0.20779166762389423 1107 14-15 0.37655243519578363 1107 16-17 0.23284762317373975 1107 18-19 0.2545138379095704 1107 20-21 0.25850646748371986 1107 22-23 0.24686514186797126 1107 24-25 0.2232489905642936 1107 26-27 0.23236594892259888 1107 28-29 0.24963321482774603 1107 30-31 0.3560090876291042 1107 32-33 0.4429928012190629 1107 34-35 0.349584184241273 1107 36-37 0.40878045189219137 1107 38-39 0.4841071817342737 1107 40-41 0.47339408045159104 1107 42-43 0.561983436817215 1107 44-45 0.6862493849606821 1107 46-47 0.7401355719937399 1107 48-49 0.8111978508250459 1107 50-51 0.7959760421622235 1107 52-53 0.913279831209934 1107 54-55 0.9294792443377347 1107 56-57 0.8744326988124129 1107 58-59 0.8869618010195097 1107 60-61 0.9345804296875855 1107 62-63 0.9448505140394801 1107 64-65 1.1190836826856412 1107 66-67 1.3525499187591663 1107 68-69 1.3746663014547664 1107 70-71 1.2785355975821204 1107 72-73 1.4114382414809725 1107 74-75 1.4205245834204483 1107 76-77 1.2972620365331906 1107 78-79 1.254457522498722 1107 80-81 1.4975242386615868 1107 82-83 1.696311780356261 1107 84-85 1.703743597152009 1107 86-87 1.8732262800265644 1107 88-89 2.1495521420194486 1107 90-91 2.271939341303991 1107 92-93 2.609708990956886 1107 94-95 2.4859579252201875 1107 96-97 2.797538586050184 1107 98-99 2.9633179767725473 1107 100-101 3.0021044524857885 1108 1 0.15017783909570426 1108 2 0.16229718379575786 1108 3 0.10676263348180726 1108 4 0.06506989445469458 1108 5 0.019782843183726584 1108 6 0.08940775695584335 1108 7 0.11049754536207246 1108 8 0.06935600579167556 1108 9 0.10359763115361176 1108 10-11 0.10348387248829738 1108 12-13 0.14070054820444966 1108 14-15 0.26674138628307276 1108 16-17 0.2561133503458848 1108 18-19 0.19806246264425198 1108 20-21 0.20041643120347175 1108 22-23 0.2651144299696071 1108 24-25 0.31359199654789904 1108 26-27 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-0.01428383613131956 2316 56-57 0.04278114383853193 2316 58-59 0.13014500574470844 2316 60-61 0.2413817040187567 2316 62-63 0.2961959472747324 2316 64-65 0.5529734020302755 2316 66-67 0.7364189276907425 2316 68-69 0.9403050207190233 2316 70-71 0.9981057671195543 2316 72-73 1.1930602259720544 2316 74-75 1.561220196989769 2316 76-77 1.6046479172526027 2316 78-79 1.5564695335889986 2316 80-81 1.537577054170253 2316 82-83 1.7432528254521173 2316 84-85 1.9384963663984571 2316 86-87 2.0281707843467984 2316 88-89 2.3253626643648566 2316 90-91 2.647989655108674 2316 92-93 2.7342036505498406 2316 94-95 3.1866836756839128 2316 96-97 3.5197700199322313 2316 98-99 3.8335228595569895 2316 100-101 4.146440842754139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 10.0 5 33.0 6 74.0 7 182.0 8 507.0 9 991.0 10 1798.0 11 2944.0 12 4522.0 13 6841.0 14 9187.0 15 12801.0 16 17030.0 17 23719.0 18 24458.0 19 20127.0 20 18959.0 21 20082.0 22 21958.0 23 23821.0 24 26906.0 25 30236.0 26 34246.0 27 40254.0 28 47927.0 29 57555.0 30 69324.0 31 83333.0 32 101668.0 33 129109.0 34 170192.0 35 236473.0 36 336988.0 37 430140.0 38 246109.0 39 12910.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16608075467997 18.75120116743534 15.134864318335547 22.947853759549144 2 15.735301980445207 19.743738281993938 40.16177973617336 24.359180001387493 3 19.594708930656665 23.56924061163906 29.74969525409336 27.086355203610914 4 13.112802869676516 16.727821220460804 36.34855842406372 33.810817485798964 5 13.798685788898208 37.12128852135327 34.16440200564042 14.915623684108095 6 32.756330900733495 35.91619238480496 16.7767212654114 14.550755449050154 7 28.907190569327962 32.69627462476082 19.98004791857289 18.416486887338337 8 26.720941657992448 34.75416760183299 19.120336907288642 19.404553832885927 9 26.089113700797423 17.057483289689994 19.95234651233994 36.90105649717265 10-11 25.299822437078706 26.511416558018542 26.657235523364392 21.53152548153836 12-13 26.23893103498551 23.710349324894196 27.27128336152809 22.7794362785922 14-15 22.787698101503786 25.195312586290765 25.75252173367506 26.264467578530393 16-17 22.17851505850101 28.411178204687815 26.77319413410444 22.63711260270674 18-19 22.156508085320333 26.712395341038768 29.250785204533138 21.880311369107762 20-21 22.650428134732216 25.79064982550765 29.654730909776916 21.904191129983218 22-23 22.442157685074754 25.684623223344 29.647857563996727 22.225361527584518 24-25 22.337740830673635 25.951923994528624 29.57319821013901 22.13713696465873 26-27 21.824834627206215 26.231970151489275 29.49241493212 22.45078028918451 28-29 21.972318033350373 26.680828108577142 29.30079795071447 22.046055907358014 30-31 22.095936110925795 26.143743348017583 29.213496549253055 22.546823991803564 32-33 21.78488068734832 26.138088196505908 29.329714330905006 22.747316785240763 34-35 22.56469634869043 26.325387020433123 29.03044289736425 22.079473733512195 36-37 22.59830194189384 25.858163504695792 29.129114534757083 22.41442001865328 38-39 22.056975615963353 26.316582452754496 29.045957113645482 22.580484817636673 40-41 22.621818381855373 26.085363943468927 29.083134151387707 22.209683523287996 42-43 22.247612836722958 26.1054030093622 28.87986520588158 22.767118948033264 44-45 22.27296324687575 26.385791825815332 28.98360612279613 22.35763880451279 46-47 22.760708617149472 26.699124887200686 28.19902161439148 22.34114488125836 48-49 22.463786044411496 26.738219060246358 28.18096749922573 22.617027396116413 50-51 22.57174200315186 26.959962852723507 28.113834665460235 22.354460478664397 52-53 22.89965242908639 26.672941823070634 27.991343200100026 22.436062547742956 54-55 22.518393601196067 26.923889171401015 28.028212137103846 22.529505090299075 56-57 22.531294415582064 27.127629258870307 27.865273084173836 22.475803241373796 58-59 22.53887143499028 26.703912613771262 27.840310891954356 22.9169050592841 60-61 22.200490142584925 26.927975901985622 28.149245633714955 22.7222883217145 62-63 22.280457519429643 26.89389035986645 28.333281052635765 22.49237106806814 64-65 22.097570803159638 26.98976285033774 28.08034556510206 22.83232078140056 66-67 22.046171229631234 27.00812596852775 27.975112909763865 22.970589892077154 68-69 22.52143451064458 26.682221395542822 28.12586429478405 22.670479799028545 70-71 22.44982488911706 26.83811200999548 27.650929587620166 23.061133513267297 72-73 22.38470722986981 26.878410902074183 27.690676398242044 23.046205469813966 74-75 22.296804441768064 27.28831508736988 27.44190988604428 22.972970584817773 76-77 22.339770338995407 26.846462194649433 27.854338318555566 22.959429147799593 78-79 22.43199790759394 27.088308283515723 27.706796299233506 22.772897509656833 80-81 22.61305111770977 27.054023927476216 27.473167852407617 22.8597571024064 82-83 22.49450601885847 27.159618377068696 27.562664963024424 22.783210641048413 84-85 22.405130291900335 27.246535100102015 27.50235161206376 22.845982995933888 86-87 22.537227772466117 27.172385882253142 27.333672356722115 22.956713988558626 88-89 22.44838901080355 27.31654666405705 27.045916737613783 23.189147587525618 90-91 22.383724269562645 27.28400570830222 27.259484750615233 23.0727852715199 92-93 22.47474290046536 26.913882204077627 27.358297587326806 23.25307730813021 94-95 22.298272262088112 26.802779154748873 27.44775388769748 23.45119469546554 96-97 22.490216141753507 26.486974147993614 27.4993350775949 23.523474632657983 98-99 22.131567983536442 26.754499711278008 27.20777335919976 23.90615894598579 100-101 22.49150954108004 26.136630227756807 27.462674895501195 23.90918533566196 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 582.0 1 408.5 2 333.0 3 480.5 4 432.5 5 376.5 6 727.5 7 1740.0 8 2878.0 9 3323.0 10 2830.0 11 2170.5 12 2089.5 13 2269.5 14 2442.0 15 2606.0 16 2759.5 17 2955.0 18 3210.0 19 3465.0 20 4007.0 21 4832.5 22 5629.5 23 6803.5 24 8540.0 25 10778.5 26 13808.0 27 17247.5 28 20148.0 29 22850.0 30 26023.5 31 31087.0 32 37844.5 33 40734.5 34 41076.0 35 43830.5 36 48318.0 37 52798.0 38 56394.5 39 59117.0 40 62218.0 41 66508.5 42 69229.5 43 71333.5 44 74220.0 45 77699.0 46 79542.5 47 81788.5 48 83723.0 49 82406.0 50 80578.5 51 79453.5 52 78838.5 53 79576.0 54 81718.0 55 77864.5 56 71332.5 57 65845.0 58 61088.5 59 54432.5 60 43775.5 61 36269.0 62 30580.5 63 22657.0 64 15705.0 65 11599.0 66 8663.0 67 6777.0 68 5246.5 69 3953.5 70 3037.0 71 1997.5 72 1309.5 73 962.0 74 649.0 75 442.5 76 314.0 77 190.5 78 115.0 79 55.5 80 30.5 81 17.5 82 9.0 83 6.0 84 4.0 85 2.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015286581429984587 3 0.002739214013465446 4 0.0014137878779176495 5 0.11482608420962409 6 4.418087118492655E-5 7 0.0 8 4.8598958303419204E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2090435592463273E-5 18-19 0.0 20-21 0.0 22-23 2.8717566270202257E-4 24-25 3.092660982944858E-4 26-27 0.005633061076078135 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010382504728457738 36-37 6.185321965889716E-4 38-39 0.00614114109470479 40-41 0.009167530770872259 42-43 0.005279614106598722 44-45 0.003446107952424271 46-47 2.2090435592463273E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2090435592463273E-5 68-69 7.510748101437513E-4 70-71 0.0 72-73 2.2090435592463273E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016346922338422824 84-85 0.003622831437163977 86-87 0.0026066713999106663 88-89 0.0 90-91 0.002341586172801107 92-93 0.0 94-95 4.418087118492655E-5 96-97 4.418087118492655E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2263423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.06748775702167 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6452972159088 40.14305883881505 2 13.753703056564612 14.597489371359082 3 4.520216713956528 7.196296353809181 4 1.9612758987918884 4.163199389891208 5 1.0530714595824506 2.7941928394330313 6 0.6596765471734544 2.1004426254432973 7 0.45578485726728973 1.6931150133007438 8 0.3147312195914285 1.3361596113936505 9 0.23461835886203755 1.1205546197835325 >10 1.2515120167313694 12.240814483705213 >50 0.09562930817297204 3.5183474886413686 >100 0.048146521268741554 4.723546175792266 >500 0.003418550937787221 1.2187917443879352 >1k 0.0027515166084627236 2.3845752292086275 >5k 1.6675858233107415E-4 0.7694162150357905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9453 0.41764177531111063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7936 0.3506193937235771 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4976 0.2198440150161945 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2640 0.11663749992820609 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2588 0.11434009462658991 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2577 0.11385410504355571 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2388 0.10550392038960459 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2335 0.1031623342168035 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2316 0.10232289766428988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.767234847397062E-4 14-15 0.0 0.0 0.0 0.0 2.2090435592463271E-4 16-17 0.0 0.0 0.0 0.0 2.6508522710955927E-4 18-19 0.0 0.0 0.0 0.0 3.092660982944858E-4 20-21 0.0 0.0 0.0 4.418087118492655E-5 3.534469694794124E-4 22-23 0.0 0.0 0.0 1.546330491472429E-4 3.534469694794124E-4 24-25 0.0 0.0 0.0 4.197182762568022E-4 3.534469694794124E-4 26-27 0.0 0.0 0.0 8.173461169211412E-4 3.534469694794124E-4 28-29 0.0 0.0 0.0 0.003379836645646881 3.534469694794124E-4 30-31 0.0 0.0 0.0 0.01126612215215627 3.534469694794124E-4 32-33 0.0 0.0 0.0 0.028828018448164572 3.534469694794124E-4 34-35 0.0 0.0 0.0 0.054232019379497336 3.534469694794124E-4 36-37 0.0 0.0 0.0 0.09832452882205403 3.534469694794124E-4 38-39 0.0 0.0 0.0 0.16176825984360854 3.534469694794124E-4 40-41 0.0 0.0 0.0 0.2281941996701456 3.534469694794124E-4 42-43 0.0 0.0 0.0 0.2961664699881551 3.9762784066433893E-4 44-45 0.0 0.0 0.0 0.3807065670005121 4.197182762568022E-4 46-47 0.0 0.0 0.0 0.47733013228194643 4.418087118492655E-4 48-49 0.0 0.0 0.0 0.5732909844956069 5.743513254040451E-4 50-51 0.0 0.0 0.0 0.6833455346172589 6.185321965889716E-4 52-53 0.0 0.0 0.0 0.7839895591765216 6.185321965889716E-4 54-55 0.0 0.0 0.0 0.8935581197151394 7.068939389588248E-4 56-57 0.0 0.0 0.0 1.019606145205735 7.731652457362145E-4 58-59 0.0 0.0 0.0 1.157273739817966 8.394365525136044E-4 60-61 0.0 0.0 0.0 1.3036007851824427 8.83617423698531E-4 62-63 0.0 0.0 0.0 1.4440075938081391 8.83617423698531E-4 64-65 0.0 0.0 0.0 1.5832877902186202 9.057078592909942E-4 66-67 0.0 0.0 0.0 1.7453211352893383 9.719791660683841E-4 68-69 0.0 0.0 0.0 1.9275672289271604 9.719791660683841E-4 70-71 0.0 0.0 0.0 2.1199307420663303 0.0010161600372533106 72-73 0.0 0.0 0.0 2.3131999630647915 0.001126612215215627 74-75 0.0 0.0 0.0 2.5096502067885673 0.00121497395758548 76-77 0.0 0.0 0.0 2.7177200196339792 0.0012370643931779433 78-79 0.0 0.0 0.0 2.9509729290547986 0.0012370643931779433 80-81 0.0 0.0 0.0 3.194630433639669 0.0012370643931779433 82-83 0.0 0.0 0.0 3.446417218522565 0.0012812452643628698 84-85 0.0 0.0 0.0 3.699750333896934 0.0012812452643628698 86-87 0.0 0.0 0.0 3.9631345974658734 0.0013254261355477963 88-89 0.0 0.0 0.0 4.243197139907123 0.001369607006732723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5880 0.0 43.51598 1 GTATCAA 9520 0.0 37.518616 1 TATCAAC 11945 0.0 29.742556 2 ATCAACG 12035 0.0 29.397028 3 TCAACGC 12205 0.0 29.025885 4 CAACGCA 12340 0.0 28.708342 5 AACGCAG 12740 0.0 27.847137 6 ACGCAGA 14245 0.0 24.738356 7 CGCAGAG 14420 0.0 24.438133 8 GTGTAAG 1135 0.0 24.303898 1 TAAGGTG 1365 0.0 23.340443 5 TGGTATC 3105 0.0 21.903738 2 GAGTACT 9405 0.0 21.814955 12-13 GCAGAGT 16190 0.0 21.561049 9 TAAGGTA 910 0.0 21.424438 4 GTACTTT 9575 0.0 21.278835 14-15 AGTACTT 9580 0.0 20.945494 12-13 AGAGTAC 13465 0.0 20.792505 10-11 TACATGG 8315 0.0 20.648514 2 GTACATG 8365 0.0 20.638802 1 >>END_MODULE