FastQCFastQC Report
Thu 26 May 2016
SRR938230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242743
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98730.44021985577482575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79930.3563939336785356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49910.2225399878630766No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28010.12489170627218545No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24840.11075722898254503No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24530.10937499303308493No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24080.10736852149354607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23120.10308804887586318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59550.044.8084871
GTATCAA100350.040.1947671
ATCAACG121500.032.740493
TATCAAC122350.032.5899932
TCAACGC124300.031.9647354
CAACGCA125450.031.6717155
AACGCAG127700.031.0695256
ACGCAGA143200.027.5646027
CGCAGAG144200.027.1099228
GCAGAGT158700.023.9445559
GAGTACT93800.023.80144312-13
GTACATG81750.023.6481691
TACATGG80200.023.1108822
TGGTATC30450.022.6311682
ACATGGG79950.022.232543
GTACTTT100850.021.99873414-15
AGAGTAC132650.021.46673210-11
CAGAGTA149100.021.1531210-11
TAAGGTG14800.020.8731335
AGTACTT98450.020.60252212-13
Produced by FastQC (version 0.11.5)