##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938230_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2242743 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99000509643771 33.0 31.0 34.0 30.0 34.0 2 32.177694457189254 34.0 31.0 34.0 30.0 34.0 3 32.26003068563808 34.0 31.0 34.0 30.0 34.0 4 35.76665449407266 37.0 35.0 37.0 35.0 37.0 5 35.77666188234675 37.0 35.0 37.0 35.0 37.0 6 35.806484737662764 37.0 36.0 37.0 35.0 37.0 7 35.75726331550249 37.0 36.0 37.0 35.0 37.0 8 35.72844280419112 37.0 36.0 37.0 35.0 37.0 9 37.38295382038869 39.0 37.0 39.0 35.0 39.0 10-11 37.30336222206468 39.0 37.0 39.0 34.0 39.0 12-13 37.21151264322305 39.0 37.0 39.0 34.0 39.0 14-15 38.438259087198134 40.0 38.0 41.0 34.0 41.0 16-17 38.299210832449376 40.0 38.0 41.0 33.5 41.0 18-19 38.44677232299911 40.0 38.0 41.0 34.0 41.0 20-21 38.425377539914294 40.0 38.0 41.0 34.0 41.0 22-23 38.34540448905648 40.0 38.0 41.0 34.0 41.0 24-25 38.248285915952025 40.0 38.0 41.0 34.0 41.0 26-27 38.081427742723974 40.0 38.0 41.0 33.0 41.0 28-29 37.8792550015762 40.0 38.0 41.0 33.0 41.0 30-31 37.66106504401084 40.0 37.5 41.0 33.0 41.0 32-33 37.41005835265119 40.0 37.0 41.0 32.0 41.0 34-35 37.18734469352931 40.0 36.5 41.0 31.0 41.0 36-37 36.93674955177655 40.0 36.0 41.0 31.0 41.0 38-39 36.65789704839119 39.0 36.0 41.0 30.0 41.0 40-41 36.44233267030596 39.0 35.0 41.0 30.0 41.0 42-43 36.27592194023123 39.0 35.0 41.0 30.0 41.0 44-45 36.013088659734976 39.0 35.0 41.0 29.0 41.0 46-47 35.64038434185281 39.0 35.0 40.5 27.5 41.0 48-49 35.25053739996068 39.0 35.0 40.0 26.0 41.0 50-51 34.534472741638254 38.5 34.0 39.5 24.0 40.5 52-53 34.64492699341832 38.0 34.0 40.0 25.5 40.5 54-55 34.75138145565497 38.0 34.0 40.0 25.0 41.0 56-57 34.46015415052014 38.0 34.0 40.0 23.5 41.0 58-59 34.14606221042715 38.0 34.0 40.0 23.0 41.0 60-61 33.73215120947875 37.5 33.5 40.0 22.0 41.0 62-63 33.21169523213315 37.0 33.0 40.0 19.5 41.0 64-65 32.705619636311425 36.0 32.0 39.0 17.5 41.0 66-67 32.184501969240344 35.5 31.5 39.0 13.5 40.0 68-69 31.59949780246778 35.0 31.0 38.5 8.0 40.0 70-71 31.064278876358102 35.0 30.5 38.0 4.5 40.0 72-73 30.442200466125634 35.0 30.0 37.0 2.0 39.0 74-75 29.769719490819945 34.0 29.0 36.5 2.0 39.0 76-77 29.12517551052439 34.0 28.5 36.0 2.0 38.5 78-79 28.421817613520588 34.0 27.0 35.0 2.0 37.5 80-81 27.779533366061116 33.5 26.0 35.0 2.0 37.0 82-83 27.06890490796315 33.0 25.5 35.0 2.0 36.5 84-85 26.435826351927084 32.5 24.5 35.0 2.0 36.0 86-87 25.77932558478613 32.0 21.5 35.0 2.0 35.5 88-89 24.989808239285555 31.5 19.0 34.0 2.0 35.0 90-91 24.188068360931233 31.0 11.5 34.0 2.0 35.0 92-93 23.316251126410826 30.5 4.5 34.0 2.0 35.0 94-95 22.38704679938807 30.0 2.0 34.0 2.0 35.0 96-97 21.20088659289094 29.0 2.0 34.0 2.0 35.0 98-99 19.727466767257773 28.0 2.0 34.0 2.0 35.0 100-101 15.949015335239036 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30665949685960214 1101 2 0.24553386474276095 1101 3 0.21555305289789572 1101 4 0.1640166620995558 1101 5 0.1625905730775088 1101 6 0.21831702904569994 1101 7 0.1561479073517873 1101 8 0.1201979899554928 1101 9 0.2296074163636277 1101 10-11 0.31257764993711845 1101 12-13 0.31731378217502026 1101 14-15 0.3059002775891386 1101 16-17 0.4435859226333463 1101 18-19 0.3465792024077885 1101 20-21 0.42580275993436345 1101 22-23 0.4088777436839166 1101 24-25 0.4298156382696945 1101 26-27 0.43640086340812445 1101 28-29 0.4813869662041128 1101 30-31 0.45099151527087855 1101 32-33 0.2863868148099371 1101 34-35 0.42564810658051755 1101 36-37 0.38443155334098833 1101 38-39 0.5155444148037844 1101 40-41 0.6401121975098718 1101 42-43 0.7692232242618928 1101 44-45 0.6598286320218136 1101 46-47 0.6656340339431495 1101 48-49 0.6350723067575643 1101 50-51 0.6987644520461487 1101 52-53 0.6713947537158731 1101 54-55 0.7082630381461072 1101 56-57 0.8061564391686531 1101 58-59 0.8315618820967643 1101 60-61 0.9152993408351406 1101 62-63 0.8665850153503101 1101 64-65 0.7989082074029525 1101 66-67 0.774273357895936 1101 68-69 1.0866322545081353 1101 70-71 1.3194503311481185 1101 72-73 1.4937753252753332 1101 74-75 1.6402511672080706 1101 76-77 1.6293142644369034 1101 78-79 1.5490123555294524 1101 80-81 1.9513565613623527 1101 82-83 2.1001405859626416 1101 84-85 2.036692117523639 1101 86-87 2.3441100022069037 1101 88-89 2.6317715202524283 1101 90-91 2.966706412535327 1101 92-93 3.1538120076026352 1101 94-95 3.3947644347204715 1101 96-97 3.509803494584059 1101 98-99 3.9158721891211066 1101 100-101 4.2326713990298295 1102 1 0.2603999713784262 1102 2 0.14405029732380825 1102 3 0.12042271800059012 1102 4 0.08382573209375721 1102 5 0.10419510141400679 1102 6 0.23871376076425577 1102 7 0.04486615620282919 1102 8 0.2279714595727711 1102 9 0.236302414250801 1102 10-11 0.2479665254198764 1102 12-13 0.22921011043574424 1102 14-15 0.3785576288747947 1102 16-17 0.4027324045792895 1102 18-19 0.24230309530091887 1102 20-21 0.3608113805016515 1102 22-23 0.37813278253925375 1102 24-25 0.28231931525010623 1102 26-27 0.27877496436320826 1102 28-29 0.1926113378963379 1102 30-31 0.19164510604561968 1102 32-33 0.3597872054925091 1102 34-35 0.37415154552626717 1102 36-37 0.5140616278196362 1102 38-39 0.4481947997538782 1102 40-41 0.5148762101628321 1102 42-43 0.6564802350924666 1102 44-45 0.688197341164198 1102 46-47 0.8545049853065549 1102 48-49 0.9474736943467903 1102 50-51 0.9151183069520101 1102 52-53 0.8576118369613965 1102 54-55 1.0265697054096776 1102 56-57 1.1415939588911357 1102 58-59 1.2199724875237337 1102 60-61 1.3074344244147866 1102 62-63 1.2954241159286894 1102 64-65 1.3472366144378611 1102 66-67 1.2607567014749534 1102 68-69 1.3525205223473584 1102 70-71 1.647674745424517 1102 72-73 1.717526544111081 1102 74-75 1.534430291568082 1102 76-77 1.811901865014736 1102 78-79 1.9745779335333005 1102 80-81 1.913414873303502 1102 82-83 2.1493578013283248 1102 84-85 2.1594896144471996 1102 86-87 2.4216238611746412 1102 88-89 2.6862877366368565 1102 90-91 2.954732886794279 1102 92-93 3.181352781957788 1102 94-95 3.4252874667579754 1102 96-97 3.6666716135533477 1102 98-99 4.176087752438871 1102 100-101 4.180242353559748 1103 1 0.5423696573483028 1103 2 0.4267642760666348 1103 3 0.40948102476090753 1103 4 0.383757104515027 1103 5 0.4744967338940853 1103 6 0.4153438363973052 1103 7 0.4978416829629353 1103 8 0.37265030918591435 1103 9 0.5420804716683776 1103 10-11 0.5291776470588516 1103 12-13 0.6577756885548354 1103 14-15 0.7729820724546741 1103 16-17 0.7993993442670586 1103 18-19 0.7049899518785949 1103 20-21 0.7718327152398032 1103 22-23 0.7676853505820063 1103 24-25 0.7663815528938827 1103 26-27 0.712010134197854 1103 28-29 0.8122839359465956 1103 30-31 0.93743344518996 1103 32-33 1.1359209101345584 1103 34-35 1.1718874249055418 1103 36-37 1.1666273747154179 1103 38-39 1.2274229504640104 1103 40-41 1.2647625030760068 1103 42-43 1.3127896288180665 1103 44-45 1.3167999012334946 1103 46-47 1.301934615667001 1103 48-49 1.300221310531235 1103 50-51 1.4671919643241722 1103 52-53 1.4114173168673574 1103 54-55 1.5287251760391243 1103 56-57 1.5542445781216259 1103 58-59 1.4902616789282845 1103 60-61 1.4608027913204111 1103 62-63 1.537635102918614 1103 64-65 1.6696719068837993 1103 66-67 1.7800572772392371 1103 68-69 1.9596558669775668 1103 70-71 2.2110522523322658 1103 72-73 2.2583134157669633 1103 74-75 2.1528215519141867 1103 76-77 2.316768000903547 1103 78-79 2.59397425283645 1103 80-81 2.7565547835912483 1103 82-83 2.7033432269651314 1103 84-85 2.9775148403566583 1103 86-87 3.0752036643140137 1103 88-89 3.368162454696023 1103 90-91 3.5816625720691952 1103 92-93 3.4809497035206824 1103 94-95 3.68507651451619 1103 96-97 4.194000716300497 1103 98-99 4.397880328374416 1103 100-101 4.346919879020518 1104 1 0.25019696139654 1104 2 0.23167218039314008 1104 3 0.1241326844707018 1104 4 0.1509886025604814 1104 5 0.16727898394378116 1104 6 0.10875220451497825 1104 7 0.09768680984294065 1104 8 0.20120824995316156 1104 9 0.27504774126355613 1104 10-11 0.2562589098959265 1104 12-13 0.312795399679203 1104 14-15 0.4283090452235072 1104 16-17 0.38119595527879113 1104 18-19 0.40629146582545417 1104 20-21 0.3775096018759925 1104 22-23 0.3457318787738828 1104 24-25 0.4183013170229444 1104 26-27 0.4971915924897772 1104 28-29 0.5373535046208104 1104 30-31 0.37385595893805856 1104 32-33 0.4279143011105049 1104 34-35 0.2410125988272256 1104 36-37 0.36388313897698765 1104 38-39 0.24137561063760415 1104 40-41 0.21743551374320447 1104 42-43 0.3009716502849784 1104 44-45 0.4541938918251631 1104 46-47 0.3382478072127455 1104 48-49 0.4287241760980507 1104 50-51 0.4674379392794492 1104 52-53 0.4516015082143312 1104 54-55 0.6511014431828315 1104 56-57 0.741329236572561 1104 58-59 0.7123025256784317 1104 60-61 0.5878873669514988 1104 62-63 0.7526412315875319 1104 64-65 0.871487732100789 1104 66-67 0.7019738942139995 1104 68-69 0.8371085768430611 1104 70-71 1.0814358738786751 1104 72-73 1.204992339771465 1104 74-75 1.354444406880063 1104 76-77 1.4376497838492845 1104 78-79 1.4971607465752719 1104 80-81 1.526713135144952 1104 82-83 1.80634006324685 1104 84-85 1.8027877317632708 1104 86-87 1.9885641625582302 1104 88-89 2.2562264578763056 1104 90-91 2.4283866328920922 1104 92-93 2.7491845640520545 1104 94-95 2.914815880447417 1104 96-97 3.1843733228068203 1104 98-99 3.360840806741873 1104 100-101 3.591576756380885 1105 1 0.2688596814936872 1105 2 0.16001158537189042 1105 3 0.1868148338331821 1105 4 0.045083927393505974 1105 5 0.15295749031901096 1105 6 0.17120666717435995 1105 7 0.22228456333414215 1105 8 0.16609896033567395 1105 9 0.2657429446636783 1105 10-11 0.20189491475324672 1105 12-13 0.20431786476723346 1105 14-15 0.35490285214518025 1105 16-17 0.33774194799281076 1105 18-19 0.32120873965666163 1105 20-21 0.3257374403702258 1105 22-23 0.26738108945998107 1105 24-25 0.23367775903873422 1105 26-27 0.30122544191296896 1105 28-29 0.3425902987920324 1105 30-31 0.18657605608438388 1105 32-33 0.3940231353545869 1105 34-35 0.3034329084811418 1105 36-37 0.4463398785034087 1105 38-39 0.4629678996479214 1105 40-41 0.5772981427656703 1105 42-43 0.5360240182266836 1105 44-45 0.5805979598275925 1105 46-47 0.712534388876378 1105 48-49 0.8122206849019378 1105 50-51 0.8909124383687015 1105 52-53 0.858174064377053 1105 54-55 1.089489421324913 1105 56-57 1.087439588727996 1105 58-59 1.0280356768624088 1105 60-61 1.0122726128531312 1105 62-63 1.125417491453959 1105 64-65 1.0864277746023063 1105 66-67 1.3468509433943225 1105 68-69 1.497942669132069 1105 70-71 1.4437309558458935 1105 72-73 1.6076031473003098 1105 74-75 1.609814018294756 1105 76-77 1.5884353029749683 1105 78-79 1.7998588340069652 1105 80-81 1.8269560009615873 1105 82-83 2.1239819879584445 1105 84-85 2.1836886121518546 1105 86-87 2.2311112178102057 1105 88-89 2.5294049642515333 1105 90-91 2.684750476243643 1105 92-93 2.8652099131716646 1105 94-95 3.22687189137638 1105 96-97 3.4464824606331774 1105 98-99 3.376680211720622 1105 100-101 3.5196163738672226 1106 1 0.13348624259874953 1106 2 0.10015246498652886 1106 3 0.08772983188742245 1106 4 0.1714537265713858 1106 5 0.20853920736288956 1106 6 0.0980076403817236 1106 7 0.1123727929535221 1106 8 0.10721762557653847 1106 9 0.06941405551317104 1106 10-11 0.14317708929615236 1106 12-13 0.21641183029294098 1106 14-15 0.38395436738588984 1106 16-17 0.21626352958558925 1106 18-19 0.3034683891581196 1106 20-21 0.26800639355096934 1106 22-23 0.2641693579945823 1106 24-25 0.1400065500836618 1106 26-27 0.16204755033271567 1106 28-29 0.1484197324285077 1106 30-31 0.17222852360325902 1106 32-33 0.26171018182066064 1106 34-35 0.2084859181968639 1106 36-37 0.2902882357254484 1106 38-39 0.2469851923917048 1106 40-41 0.608987063048346 1106 42-43 0.37851877775355547 1106 44-45 0.4569824748426541 1106 46-47 0.8532521293086361 1106 48-49 0.6843940178428412 1106 50-51 0.6595459584296606 1106 52-53 0.6552304064123575 1106 54-55 0.3461642798076099 1106 56-57 0.7572873288659352 1106 58-59 0.836502106767604 1106 60-61 0.8730285000055531 1106 62-63 0.5658011823821383 1106 64-65 0.7163980486111896 1106 66-67 0.8994629227398292 1106 68-69 0.6805784717828836 1106 70-71 0.7528501966394998 1106 72-73 1.1814169690686285 1106 74-75 1.3769587861606034 1106 76-77 1.5409374858241485 1106 78-79 1.7223664617159038 1106 80-81 1.9652562563631157 1106 82-83 1.9031238420885295 1106 84-85 2.0294380243359136 1106 86-87 1.9914699408679617 1106 88-89 2.219187758494698 1106 90-91 2.3261312468956845 1106 92-93 2.7283616203722865 1106 94-95 2.903803263252126 1106 96-97 2.91193458020426 1106 98-99 3.1639435728832375 1106 100-101 3.48809954712495 1107 1 0.2673154735622205 1107 2 0.08269339886719251 1107 3 0.13118195071808714 1107 4 0.04105117800041569 1107 5 0.058104693873261226 1107 6 0.16289981267201625 1107 7 0.13158792794217788 1107 8 0.19523817181327274 1107 9 0.19925998953240764 1107 10-11 0.09533441335822346 1107 12-13 0.10001587487137442 1107 14-15 0.1768748923742578 1107 16-17 0.22066255524067202 1107 18-19 0.15220857719067737 1107 20-21 0.17740755943903963 1107 22-23 0.14911913180736036 1107 24-25 0.15928981838880674 1107 26-27 0.22350226207874613 1107 28-29 0.33439943562900964 1107 30-31 0.433796559916388 1107 32-33 0.3580041143371133 1107 34-35 0.30882396722821426 1107 36-37 0.29768936158038173 1107 38-39 0.3683537251157176 1107 40-41 0.3197134026956263 1107 42-43 0.419850295224947 1107 44-45 0.3422767645778464 1107 46-47 0.35938528259550395 1107 48-49 0.49537218130737415 1107 50-51 0.3544376534018028 1107 52-53 0.35906450294147874 1107 54-55 0.5725851481571098 1107 56-57 0.47424844437384905 1107 58-59 0.4624240236772934 1107 60-61 0.6215661357717792 1107 62-63 0.684698438880595 1107 64-65 0.7554623048725304 1107 66-67 0.8002419521518576 1107 68-69 0.8187524527086332 1107 70-71 0.988563756478257 1107 72-73 1.1323428892918272 1107 74-75 1.3047513282954775 1107 76-77 1.4719014991151518 1107 78-79 1.4715353892282153 1107 80-81 1.7698441306344357 1107 82-83 1.8650065234463291 1107 84-85 1.9897139912131117 1107 86-87 2.09237268806487 1107 88-89 2.1781199208075037 1107 90-91 2.2597867186553877 1107 92-93 2.6239721410801984 1107 94-95 3.017389970403581 1107 96-97 3.1306017231625844 1107 98-99 3.107585772118913 1107 100-101 3.1985152450410723 1108 1 0.30229379700680425 1108 2 0.1900490318564394 1108 3 0.1686604739051205 1108 4 0.11115358289961108 1108 5 0.11046043123244687 1108 6 0.12609087659032525 1108 7 0.1419997285106689 1108 8 0.08948749646782517 1108 9 0.20566002580995502 1108 10-11 0.1812019866277197 1108 12-13 0.20195452838407135 1108 14-15 0.24788054706525742 1108 16-17 0.27834619773694413 1108 18-19 0.30207885256174904 1108 20-21 0.25324698161830383 1108 22-23 0.14874618924465466 1108 24-25 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-2.314774363222611 2316 100-101 -2.5731587839539714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5225.0 3 1543.0 4 1623.0 5 1799.0 6 2241.0 7 2777.0 8 3380.0 9 4275.0 10 5471.0 11 7089.0 12 8982.0 13 11174.0 14 13437.0 15 16732.0 16 20153.0 17 25246.0 18 24764.0 19 20179.0 20 20095.0 21 21598.0 22 23114.0 23 25706.0 24 28941.0 25 32687.0 26 36772.0 27 42571.0 28 49181.0 29 57866.0 30 69178.0 31 83656.0 32 102760.0 33 131383.0 34 173040.0 35 239345.0 36 325082.0 37 376952.0 38 212646.0 39 14080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92721936193974 18.42174226816593 14.823161971726881 22.827876398167447 2 15.509320696089238 19.45518835206275 40.78986046723248 24.245630484615532 3 19.553917011218992 23.663383828019466 29.519003763457167 27.26369539730437 4 12.913151519748025 16.445533997474893 36.69411864429464 33.947195838482436 5 13.848700945172835 37.048231482555806 34.09945288862312 15.003614683648243 6 32.557239781447294 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22.43680730060686 28-29 22.043088458206533 26.7238818154323 29.297425987436398 21.93560373892477 30-31 22.109519126602063 26.17688998191759 29.211085517724083 22.50250537375626 32-33 21.77308281748472 26.196589231940393 29.32785890316455 22.70246904741034 34-35 22.572179121682858 26.343179528313225 29.015296484398263 22.06934486560565 36-37 22.616635823403602 25.890564370376996 29.0805391686393 22.412260637580097 38-39 22.07066063463045 26.34410124793752 29.029750379046444 22.55548773838559 40-41 22.650957697369684 26.167234784035863 29.039434912391886 22.142372606202564 42-43 22.23096119620523 26.183190054547108 28.863225493105958 22.722623256141706 44-45 22.258886423550234 26.433276319125266 28.95057512552825 22.357262131796247 46-47 22.738932690195607 26.703796497307806 28.24763924522748 22.309631567269108 48-49 22.4840996278373 26.784096349754893 28.126772143613906 22.60503187879391 50-51 22.559440207045974 26.970984888738652 28.140289836717393 22.329285067497977 52-53 22.917578078140348 26.669650075967677 28.00123617497495 22.411535670917022 54-55 22.492006838253282 26.942156020898278 28.03962565758653 22.52621148326191 56-57 22.521387760635463 27.110770405332847 27.903535669754426 22.464306164277268 58-59 22.534203492692843 26.6802962596957 27.893715039235435 22.89178520837602 60-61 22.146101361501522 26.889314981377716 28.27447481771836 22.690108839402402 62-63 22.28207415718662 26.84397932469685 28.45995927105308 22.413987247063453 64-65 22.114334897161918 27.00578854421235 28.15785600884782 22.722020549777913 66-67 22.03406835967983 26.986198746962163 28.040824061893826 22.93890883146418 68-69 22.506061458754655 26.631242566713954 28.24753494731425 22.61516102721714 70-71 22.445699897471666 26.719222657735987 27.828281319194918 23.006796125597432 72-73 22.33717763416961 26.763995367395253 27.929014296054127 22.96981270238101 74-75 22.281047401341226 27.20566921717818 27.715050574690093 22.7982328067905 76-77 22.5051192134605 26.79020856781531 27.78569046060435 22.918981758119838 78-79 22.56478542802241 27.019172410779145 27.743985237515723 22.672056923682717 80-81 22.735442877373416 26.999713918053914 27.44252774247898 22.822315462093695 82-83 22.632677615513185 27.030388489619288 27.59455376047748 22.742380134390043 84-85 22.544369621163103 27.15136753283992 27.57895426871007 22.72530857728691 86-87 22.770681927680222 27.049766026081922 27.372730071751704 22.80682197448615 88-89 22.65835620464717 27.147571210310456 27.188649257038183 23.00542332800419 90-91 22.632240476642878 27.124161579722518 27.209687361452694 23.033910582181914 92-93 22.801012330813656 26.708698463665353 27.351216357838876 23.13907284768212 94-95 22.608273278771662 26.59462200751208 27.49137038584935 23.305734327866908 96-97 22.860596221433287 26.212847662034143 27.530083135603807 23.396472980928763 98-99 22.5843015839885 26.43618643931731 27.190427386106787 23.789084590587407 100-101 23.40258929504406 25.428713560128855 27.35311296817771 23.815584176649367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 630.0 1 445.5 2 379.0 3 522.0 4 444.5 5 385.5 6 726.5 7 1681.5 8 2895.0 9 3396.5 10 2941.5 11 2359.0 12 2248.0 13 2317.0 14 2440.0 15 2639.0 16 2772.5 17 2984.0 18 3260.5 19 3594.5 20 4041.5 21 4752.5 22 5713.0 23 6908.0 24 8575.5 25 10810.5 26 13865.0 27 17486.5 28 20424.0 29 23091.0 30 26203.5 31 30788.5 32 37064.0 33 40264.5 34 40871.0 35 43412.5 36 47933.0 37 52371.5 38 55527.0 39 58331.0 40 61645.5 41 65763.0 42 68527.5 43 70438.5 44 73283.0 45 76864.0 46 78503.5 47 80683.0 48 82934.5 49 81571.5 50 79563.5 51 78094.5 52 77208.5 53 77591.5 54 79515.5 55 76220.5 56 69969.5 57 64897.5 58 60140.0 59 53507.0 60 43587.5 61 36092.5 62 30340.5 63 22987.5 64 16389.5 65 12318.0 66 9232.5 67 7185.0 68 5669.0 69 4317.5 70 3282.5 71 2233.0 72 1516.0 73 1111.5 74 764.5 75 533.0 76 396.0 77 248.0 78 151.0 79 98.0 80 55.5 81 33.5 82 20.5 83 12.5 84 12.5 85 11.5 86 7.0 87 6.0 88 7.5 89 5.0 90 3.0 91 3.5 92 3.0 93 2.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46576892671162057 2 0.040307783816513974 3 0.052926260387391684 4 0.055869085312048684 5 0.08395968686559271 6 0.08110603845380411 7 0.04628261017869636 8 0.03968354822643522 9 0.044989550742104645 10-11 0.049336905744438844 12-13 0.04487808010101915 14-15 0.039037018508139364 16-17 0.03917078327744196 18-19 0.04793237566676164 20-21 0.056894615210035214 22-23 0.048110728692498425 24-25 0.0656339134711378 26-27 0.057318203646160075 28-29 0.045881315870788586 30-31 0.047330434204899983 32-33 0.046750786871255424 34-35 0.05125420077110931 36-37 0.04603737476830828 38-39 0.026909012758037816 40-41 0.024166834987334705 42-43 0.02463501167989377 44-45 0.014245947930725901 46-47 0.02722113055307719 48-49 0.025794306347182893 50-51 0.015271477828712429 52-53 0.015249183700495331 54-55 0.015940301675225383 56-57 0.015115418931192741 58-59 0.014201359674291704 60-61 0.01794677321476424 62-63 0.015940301675225383 64-65 0.017077302214297402 66-67 0.008605533491799997 68-69 0.013198123904522276 70-71 0.019440479805309836 72-73 0.016765184419258027 74-75 0.014134477289640408 76-77 0.00940812210761554 78-79 0.013465653443127456 80-81 0.01687665506034352 82-83 0.014045300776772017 84-85 0.012217182262969943 86-87 0.003500178130084454 88-89 0.029851837682694806 90-91 0.032504838940529523 92-93 0.017523184778639372 94-95 0.03361954535138444 96-97 0.02777848375850465 98-99 0.024746482320979264 100-101 0.1502178359268093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2242743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.68029833939085 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86022040767678 42.02739782323104 2 13.238080004254549 14.477243281467263 3 4.15898357357916 6.822433877758032 4 1.877620693826318 4.106754388265485 5 1.0175988438328956 2.7821304185300963 6 0.6046624512071546 1.9837873935980748 7 0.41068481430277615 1.5719457718673189 8 0.296140799877106 1.2954453830196797 9 0.21831663104205085 1.0743856666047054 >10 1.178789560479362 11.804664019277133 >50 0.08917288529154163 3.3434649124680145 >100 0.04375267473840995 4.408869792846654 >500 0.0032748821326059924 1.2214021521429752 >1k 0.002538033652769606 2.2802521210791222 >5k 1.6374410663029716E-4 0.7998229978443036 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9873 0.44021985577482575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7993 0.3563939336785356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4991 0.2225399878630766 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2801 0.12489170627218545 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2484 0.11075722898254503 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2453 0.10937499303308493 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2408 0.10736852149354607 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2312 0.10308804887586318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4588256434196876E-5 2 0.0 0.0 0.0 0.0 4.4588256434196876E-5 3 0.0 0.0 0.0 0.0 4.4588256434196876E-5 4 0.0 0.0 0.0 0.0 4.4588256434196876E-5 5 0.0 0.0 0.0 0.0 4.4588256434196876E-5 6 0.0 0.0 0.0 0.0 4.4588256434196876E-5 7 0.0 0.0 0.0 0.0 4.4588256434196876E-5 8 0.0 0.0 0.0 0.0 4.4588256434196876E-5 9 0.0 0.0 0.0 4.4588256434196876E-5 4.4588256434196876E-5 10-11 0.0 0.0 0.0 4.4588256434196876E-5 4.4588256434196876E-5 12-13 0.0 0.0 0.0 8.917651286839375E-5 8.917651286839375E-5 14-15 0.0 0.0 0.0 8.917651286839375E-5 1.3376476930259063E-4 16-17 0.0 0.0 0.0 8.917651286839375E-5 2.2294128217098438E-4 18-19 0.0 0.0 0.0 8.917651286839375E-5 2.2294128217098438E-4 20-21 0.0 0.0 0.0 1.1147064108549219E-4 2.2294128217098438E-4 22-23 0.0 0.0 0.0 2.6752953860518125E-4 2.2294128217098438E-4 24-25 0.0 0.0 0.0 4.904708207761656E-4 2.2294128217098438E-4 26-27 0.0 0.0 0.0 8.025886158155438E-4 2.2294128217098438E-4 28-29 0.0 0.0 0.0 0.003767707668689636 2.2294128217098438E-4 30-31 0.0 0.0 0.0 0.011971946852581862 2.6752953860518125E-4 32-33 0.0 0.0 0.0 0.0313455442732404 4.4588256434196876E-4 34-35 0.0 0.0 0.0 0.0583883218005808 4.904708207761656E-4 36-37 0.0 0.0 0.0 0.10043504761802846 4.904708207761656E-4 38-39 0.0 0.0 0.0 0.16620272585846885 4.904708207761656E-4 40-41 0.0 0.0 0.0 0.2335532872023232 4.904708207761656E-4 42-43 0.0 0.0 0.0 0.3040696147530056 4.904708207761656E-4 44-45 0.0 0.0 0.0 0.3881407722596838 5.573532054274609E-4 46-47 0.0 0.0 0.0 0.4859228186198775 5.796473336445593E-4 48-49 0.0 0.0 0.0 0.5871158665972873 5.796473336445593E-4 50-51 0.0 0.0 0.0 0.6975609777847931 6.688238465129531E-4 52-53 0.0 0.0 0.0 0.7979737312746044 6.911179747300515E-4 54-55 0.0 0.0 0.0 0.9075270773334261 7.580003593813468E-4 56-57 0.0 0.0 0.0 1.0380591980445373 7.580003593813468E-4 58-59 0.0 0.0 0.0 1.1740087919124038 7.580003593813468E-4 60-61 0.0 0.0 0.0 1.3174938011176494 7.580003593813468E-4 62-63 0.0 0.0 0.0 1.4546472779092388 7.580003593813468E-4 64-65 0.0 0.0 0.0 1.5976641104219254 7.580003593813468E-4 66-67 0.0 0.0 0.0 1.7642235423318677 7.580003593813468E-4 68-69 0.0 0.0 0.0 1.9472806291224631 7.580003593813468E-4 70-71 0.0 0.0 0.0 2.134618188530741 7.580003593813468E-4 72-73 0.0 0.0 0.0 2.3252329847869326 8.025886158155437E-4 74-75 0.0 0.0 0.0 2.520239724301893 8.471768722497406E-4 76-77 0.0 0.0 0.0 2.726594175079356 8.917651286839375E-4 78-79 0.0 0.0 0.0 2.9595232266916005 9.363533851181343E-4 80-81 0.0 0.0 0.0 3.2007902822570395 9.363533851181343E-4 82-83 0.0 0.0 0.0 3.4461148691579906 9.809416415523312E-4 84-85 0.0 0.0 0.0 3.6955415756508883 0.0010032357697694295 86-87 0.0 0.0 0.0 3.9489812252228633 0.001025529897986528 88-89 0.0 4.4588256434196876E-5 0.0 4.226253297858916 0.001025529897986528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5955 0.0 44.808487 1 GTATCAA 10035 0.0 40.194767 1 ATCAACG 12150 0.0 32.74049 3 TATCAAC 12235 0.0 32.589993 2 TCAACGC 12430 0.0 31.964735 4 CAACGCA 12545 0.0 31.671715 5 AACGCAG 12770 0.0 31.069525 6 ACGCAGA 14320 0.0 27.564602 7 CGCAGAG 14420 0.0 27.109922 8 GCAGAGT 15870 0.0 23.944555 9 GAGTACT 9380 0.0 23.801443 12-13 GTACATG 8175 0.0 23.648169 1 TACATGG 8020 0.0 23.110882 2 TGGTATC 3045 0.0 22.631168 2 ACATGGG 7995 0.0 22.23254 3 GTACTTT 10085 0.0 21.998734 14-15 AGAGTAC 13265 0.0 21.466732 10-11 CAGAGTA 14910 0.0 21.15312 10-11 TAAGGTG 1480 0.0 20.873133 5 AGTACTT 9845 0.0 20.602522 12-13 >>END_MODULE