FastQCFastQC Report
Thu 26 May 2016
SRR938230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242743
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94800.4226966709961864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81060.36143240665559984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50790.22646375442928593No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26850.1197194685258186No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25660.11441346601014916No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25310.11285287703495228No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24370.10866158093013778No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23120.10308804887586318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.044.0183071
GTATCAA91400.038.097151
ATCAACG115150.030.0285473
TATCAAC116250.029.8716432
TCAACGC118050.029.5309624
CAACGCA118700.029.3292395
AACGCAG122750.028.4002466
GTAAGGT13000.027.400944
TAAGGTG14050.026.705355
ACGCAGA137500.025.2155087
CGCAGAG138300.024.9666218
AGGTAAG14600.023.1015012
GTACATG81000.022.755241
GAGTACT90150.022.6542812-13
GCAGAGT153500.022.3087089
TACATGG80100.022.2399312
GGTAAGG15550.021.9922163
AAGGTAA15550.021.3846591
GTACTTT95100.021.3752314-15
AGAGTAC128500.021.3265310-11
Produced by FastQC (version 0.11.5)