##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938230_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2242743 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54181063099963 34.0 31.0 34.0 31.0 34.0 2 32.77079986427335 34.0 31.0 34.0 31.0 34.0 3 32.72701820939805 34.0 31.0 34.0 31.0 34.0 4 36.24054249639838 37.0 35.0 37.0 35.0 37.0 5 36.2077157302464 37.0 37.0 37.0 35.0 37.0 6 36.26819167421323 37.0 37.0 37.0 35.0 37.0 7 36.22974188304233 37.0 37.0 37.0 35.0 37.0 8 36.22256674081694 37.0 37.0 37.0 35.0 37.0 9 37.93720858787654 39.0 38.0 39.0 35.0 39.0 10-11 37.90225005718444 39.0 38.0 39.0 35.0 39.0 12-13 37.84847751168992 39.0 38.0 39.0 35.0 39.0 14-15 39.14173291366866 40.0 38.5 41.0 36.0 41.0 16-17 39.08310403822462 40.0 38.0 41.0 36.0 41.0 18-19 39.083627281413875 40.0 38.5 41.0 36.0 41.0 20-21 39.08610148376341 40.0 39.0 41.0 36.0 41.0 22-23 39.11534357703937 40.0 39.0 41.0 36.0 41.0 24-25 39.067994861649325 40.0 39.0 41.0 35.0 41.0 26-27 38.90296770517175 40.0 38.0 41.0 35.0 41.0 28-29 38.8314956283444 40.0 38.0 41.0 35.0 41.0 30-31 38.61752929336977 40.0 38.0 41.0 35.0 41.0 32-33 38.418724749113025 40.0 38.0 41.0 34.0 41.0 34-35 38.21649582676214 40.0 38.0 41.0 34.0 41.0 36-37 38.13920587423526 40.0 38.0 41.0 34.0 41.0 38-39 38.09467669724083 40.0 38.0 41.0 34.0 41.0 40-41 37.97375401461514 40.0 38.0 41.0 33.0 41.0 42-43 37.78470649557261 40.0 37.5 41.0 33.0 41.0 44-45 37.540334759711655 40.0 37.0 41.0 33.0 41.0 46-47 37.19204028281439 40.0 37.0 41.0 32.0 41.0 48-49 36.82156849893189 40.0 36.0 41.0 31.0 41.0 50-51 36.5214112807397 39.5 36.0 41.0 30.5 41.0 52-53 36.069486115885766 39.0 35.0 40.5 29.5 41.0 54-55 35.76124348621309 39.0 35.0 40.0 28.5 41.0 56-57 35.489430353812274 38.0 35.0 40.0 28.0 41.0 58-59 35.11779659996709 38.0 34.5 40.0 27.5 41.0 60-61 34.70956993289022 38.0 34.0 40.0 26.0 41.0 62-63 34.28604592679589 37.0 34.0 40.0 26.0 41.0 64-65 33.85792732381731 36.5 33.0 39.5 25.5 41.0 66-67 33.358260175151585 36.0 33.0 39.0 23.5 40.5 68-69 32.837401788791674 35.0 32.0 39.0 22.5 40.0 70-71 32.25586725719353 35.0 31.5 38.0 21.0 40.0 72-73 31.798778772244525 35.0 31.0 37.0 20.0 39.5 74-75 31.254022418083572 35.0 31.0 36.5 18.0 39.0 76-77 30.04512264668756 33.5 29.5 35.0 18.0 37.5 78-79 30.731802529313434 34.0 30.5 36.0 18.0 38.0 80-81 30.55301543690026 34.0 31.0 35.5 15.5 37.0 82-83 30.110526484755496 34.0 30.0 35.0 8.5 37.0 84-85 29.620847774354885 34.0 29.5 35.0 6.0 36.0 86-87 29.109853870907187 34.0 29.0 35.0 2.0 36.0 88-89 28.512166574591916 34.0 29.0 35.0 2.0 35.5 90-91 28.004347132061053 33.0 27.5 35.0 2.0 35.0 92-93 27.402439334333003 33.0 26.5 35.0 2.0 35.0 94-95 26.688919996629128 33.0 25.0 35.0 2.0 35.0 96-97 25.91316793765492 32.0 24.0 34.0 2.0 35.0 98-99 24.945507131222794 32.0 18.0 34.0 2.0 35.0 100-101 21.466890990184787 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05424464808075413 1101 2 0.09956722458825595 1101 3 0.141177914057657 1101 4 0.0846002263591501 1101 5 -0.013895196158678402 1101 6 0.06678516416724278 1101 7 0.08341040086904172 1101 8 0.12465549957784106 1101 9 0.08425014691869848 1101 10-11 0.13808417983342736 1101 12-13 0.02657130918744599 1101 14-15 0.12370654190065977 1101 16-17 0.211472950139175 1101 18-19 0.23083365144172774 1101 20-21 0.21106633902391536 1101 22-23 0.0963154601538534 1101 24-25 0.17734692306086686 1101 26-27 0.18749375001201685 1101 28-29 0.0852376442864724 1101 30-31 0.11281100867474692 1101 32-33 0.07160252823523905 1101 34-35 0.010287131705730701 1101 36-37 0.09892015126212783 1101 38-39 0.1344929286867611 1101 40-41 0.2661410975632137 1101 42-43 0.2681835656881475 1101 44-45 0.2993343414323846 1101 46-47 0.13771646067410614 1101 48-49 0.07947588030461361 1101 50-51 0.1316422187902475 1101 52-53 0.08253316396915267 1101 54-55 0.20199417307394896 1101 56-57 0.32205689916080615 1101 58-59 0.2773912143819146 1101 60-61 0.32560953057906517 1101 62-63 0.40057245379981765 1101 64-65 0.22959227921210612 1101 66-67 0.32548674199183125 1101 68-69 0.4792185059166769 1101 70-71 0.5659790882485183 1101 72-73 0.41595622963108525 1101 74-75 0.574739546715687 1101 76-77 0.6440175993622894 1101 78-79 0.6798766340356828 1101 80-81 0.7630623587196723 1101 82-83 0.7954255746865435 1101 84-85 0.9851041487166761 1101 86-87 1.0000170790445786 1101 88-89 1.2365751287047182 1101 90-91 1.2821017429179022 1101 92-93 1.5305044857144203 1101 94-95 1.7139776752911864 1101 96-97 1.7695756323271894 1101 98-99 2.1711470838704905 1101 100-101 2.4777875235376854 1102 1 0.027273573659975625 1102 2 0.06143199687753054 1102 3 0.22538977364340695 1102 4 0.07972379146327313 1102 5 -0.036440791261718175 1102 6 0.02066158050079281 1102 7 0.05876289417295055 1102 8 0.06282708762937261 1102 9 0.09221666483189495 1102 10-11 0.1602298657827177 1102 12-13 0.10806378652775095 1102 14-15 0.15587971102647202 1102 16-17 0.1242373405902768 1102 18-19 0.23316486248129564 1102 20-21 0.21928672418147244 1102 22-23 0.15487226035650536 1102 24-25 0.10430740603101896 1102 26-27 0.1048383969072475 1102 28-29 0.09741590167619307 1102 30-31 0.21282514880729053 1102 32-33 0.2730145214698112 1102 34-35 0.06590589626215859 1102 36-37 0.037130228863617276 1102 38-39 0.1969278922663662 1102 40-41 0.10547397812113957 1102 42-43 0.18425778445947572 1102 44-45 0.29077246388484923 1102 46-47 0.28369422081159 1102 48-49 0.2534909572891735 1102 50-51 0.4289597974317658 1102 52-53 0.282650434257512 1102 54-55 0.24035160286737067 1102 56-57 0.2930221297214004 1102 58-59 0.2926955875591233 1102 60-61 0.5008237890737917 1102 62-63 0.3500777129464083 1102 64-65 0.4434396727871288 1102 66-67 0.512290722630226 1102 68-69 0.6175949894022068 1102 70-71 0.6647828548740904 1102 72-73 0.5861089758195277 1102 74-75 0.6194395629850256 1102 76-77 0.7457932506000127 1102 78-79 0.8101391928209587 1102 80-81 0.8903726871310731 1102 82-83 0.9867849709956964 1102 84-85 1.0029701242820899 1102 86-87 0.8329478644606993 1102 88-89 0.9914698529612131 1102 90-91 1.1539378200192942 1102 92-93 1.4761487789425516 1102 94-95 1.468978587029465 1102 96-97 1.6793351081268177 1102 98-99 1.8835845588249853 1102 100-101 2.325254528985184 1103 1 0.3803103440188238 1103 2 0.3187742480730833 1103 3 0.3459766821571364 1103 4 0.22884233660823838 1103 5 0.15587124456361323 1103 6 0.17522813777286217 1103 7 0.21751536144609673 1103 8 0.21499664366602644 1103 9 0.3554797510749239 1103 10-11 0.40546425130217756 1103 12-13 0.3343778092021523 1103 14-15 0.523671721063721 1103 16-17 0.4586109463986787 1103 18-19 0.501553144415297 1103 20-21 0.4611742742327536 1103 22-23 0.5218307803835316 1103 24-25 0.4786091080073831 1103 26-27 0.47173502180788773 1103 28-29 0.514291987943416 1103 30-31 0.4426588169017265 1103 32-33 0.4750174105295528 1103 34-35 0.5479685645346777 1103 36-37 0.6731446915363577 1103 38-39 0.7112669298793648 1103 40-41 0.7235503334382543 1103 42-43 0.7387756012585811 1103 44-45 0.7261347163586294 1103 46-47 0.8195195543161873 1103 48-49 0.8202649398667035 1103 50-51 0.905782632172702 1103 52-53 0.9324431553906507 1103 54-55 0.7753184711353072 1103 56-57 0.8578837235317636 1103 58-59 1.1324323517749875 1103 60-61 1.1617584085847525 1103 62-63 0.990806288390516 1103 64-65 0.7343118635395385 1103 66-67 0.7766260372280982 1103 68-69 0.9808863395350258 1103 70-71 0.97512517370442 1103 72-73 0.9539821165612921 1103 74-75 0.9858652264728569 1103 76-77 0.7161578951110172 1103 78-79 1.0529385713256367 1103 80-81 1.2171428101829314 1103 82-83 1.2015063002113742 1103 84-85 1.3836955785985232 1103 86-87 1.39903727763285 1103 88-89 1.499289270594776 1103 90-91 1.523094315098401 1103 92-93 1.7873810535124335 1103 94-95 1.4870437422760894 1103 96-97 1.8722062167228266 1103 98-99 2.240269573751174 1103 100-101 2.938881260730156 1104 1 0.11388137032240309 1104 2 0.1370915143554683 1104 3 0.21314606531489488 1104 4 0.05992959560247613 1104 5 0.07503741431118272 1104 6 0.05952300956773371 1104 7 0.03929532566792204 1104 8 0.03501751426829003 1104 9 0.07039477790855386 1104 10-11 0.12194581560350315 1104 12-13 0.025577371913342972 1104 14-15 0.1195270016573744 1104 16-17 0.16678609381801124 1104 18-19 0.19235185905122876 1104 20-21 0.24321603200125708 1104 22-23 0.1795251585720976 1104 24-25 0.18703975466477374 1104 26-27 0.23930649533865989 1104 28-29 0.14861377618962734 1104 30-31 0.13765774024367516 1104 32-33 0.2986256229458988 1104 34-35 0.18871344626793274 1104 36-37 0.1727020194367057 1104 38-39 0.16944663239046776 1104 40-41 0.25466078199055886 1104 42-43 0.25789539189000266 1104 44-45 0.1805610232558763 1104 46-47 0.09621156385703244 1104 48-49 -0.02483336729555674 1104 50-51 -0.029704017267434324 1104 52-53 0.052638775876964417 1104 54-55 -0.003976980398512353 1104 56-57 0.23078299802987345 1104 58-59 0.24545867192340864 1104 60-61 0.22113899892463706 1104 62-63 0.25549356063592654 1104 64-65 0.34671413611622626 1104 66-67 0.3435556569193494 1104 68-69 0.5401195105414516 1104 70-71 0.5463556282197573 1104 72-73 0.45208264329554027 1104 74-75 0.5031252973666476 1104 76-77 0.4591695561459339 1104 78-79 0.682296137747155 1104 80-81 0.760613707287547 1104 82-83 0.6891860192864421 1104 84-85 0.8111155746231695 1104 86-87 0.9178647141928451 1104 88-89 1.0058038800163622 1104 90-91 1.1019197639819893 1104 92-93 1.4457011356697294 1104 94-95 1.5931844200737437 1104 96-97 1.8136919220166696 1104 98-99 2.158485799816237 1104 100-101 2.459159701066735 1105 1 0.08506382326715567 1105 2 0.0826668027586237 1105 3 0.15193628996577502 1105 4 -0.003003009133408341 1105 5 0.030547432526120133 1105 6 0.0647825724092641 1105 7 0.07458676464788994 1105 8 0.07650203157248114 1105 9 0.055800060240066784 1105 10-11 0.026761389374001965 1105 12-13 0.08759922983684021 1105 14-15 0.06928034106235259 1105 16-17 0.03426484305905575 1105 18-19 0.07348422092128715 1105 20-21 0.05430057966367485 1105 22-23 0.09578578395157678 1105 24-25 0.08198131507764117 1105 26-27 0.02252219054206961 1105 28-29 0.08862212470207709 1105 30-31 0.04999456477312236 1105 32-33 0.07387987309768818 1105 34-35 0.05863603278888263 1105 36-37 0.0717267613917798 1105 38-39 0.18503937677419202 1105 40-41 0.22539545108588754 1105 42-43 0.19866649085782484 1105 44-45 0.2927536462066911 1105 46-47 0.2527006348952838 1105 48-49 0.300944562273358 1105 50-51 0.30314358443263245 1105 52-53 0.2980774522582692 1105 54-55 0.3880046528741019 1105 56-57 0.2381714315453607 1105 58-59 0.28744789475279475 1105 60-61 0.2923935526587016 1105 62-63 0.24512622608826717 1105 64-65 0.3989985927039612 1105 66-67 0.4519610910419942 1105 68-69 0.39683247350441064 1105 70-71 0.447588931194872 1105 72-73 0.41054120431557806 1105 74-75 0.28272836354758724 1105 76-77 0.387918797190256 1105 78-79 0.5856924644016779 1105 80-81 0.5833102768078895 1105 82-83 0.618937081824388 1105 84-85 0.6309698551331913 1105 86-87 0.7376355095783929 1105 88-89 0.9434632607085298 1105 90-91 1.0052060420633495 1105 92-93 1.1482815849714854 1105 94-95 1.0936284091447845 1105 96-97 1.33568266275741 1105 98-99 1.6099961216134453 1105 100-101 2.0251508971808825 1106 1 0.0834197372350971 1106 2 -0.00981569357059442 1106 3 0.16960510514466876 1106 4 0.0769154324267376 1106 5 0.11758179891121756 1106 6 0.020242638532430135 1106 7 0.04430729454883675 1106 8 -0.033968123074615164 1106 9 0.06995342542501248 1106 10-11 0.03716998276888006 1106 12-13 0.04509662186347185 1106 14-15 -0.029949775388971034 1106 16-17 0.05169616097673213 1106 18-19 -0.08630197431535436 1106 20-21 -0.1296690501185651 1106 22-23 0.010556560261044012 1106 24-25 -2.5037433316299484E-4 1106 26-27 -0.02278664582762957 1106 28-29 -0.06770421370331547 1106 30-31 -0.02250791239280403 1106 32-33 -0.01581724227120418 1106 34-35 -0.20942888367421375 1106 36-37 -0.036958280450257064 1106 38-39 -0.023047466286307383 1106 40-41 -0.1086712214671195 1106 42-43 -0.07933447711724284 1106 44-45 -0.031618559828110904 1106 46-47 -0.3115008837791109 1106 48-49 -0.18656577968999954 1106 50-51 -0.11588660919508698 1106 52-53 -0.26549823456254984 1106 54-55 -0.25988869327836284 1106 56-57 -0.07395842427214205 1106 58-59 -0.07677643346924157 1106 60-61 -0.23517084547990663 1106 62-63 -0.0291679860094618 1106 64-65 -0.2037355280901707 1106 66-67 -0.1422815829237294 1106 68-69 -0.37984815685664586 1106 70-71 -0.07728083364605709 1106 72-73 -0.07341730683412351 1106 74-75 -0.0895017888757863 1106 76-77 0.11342183355799662 1106 78-79 0.3521840126058571 1106 80-81 0.3921670851717174 1106 82-83 0.4222284423198879 1106 84-85 0.46019297338638765 1106 86-87 0.5929686107285193 1106 88-89 0.8053101636788433 1106 90-91 0.6581228027478652 1106 92-93 0.571884725004125 1106 94-95 0.9587876018012551 1106 96-97 1.038779610692778 1106 98-99 1.4767225509300097 1106 100-101 2.024543868897041 1107 1 0.06970453911727503 1107 2 0.07228225827262236 1107 3 0.15249632895761067 1107 4 0.07429018260457099 1107 5 0.05268654714899412 1107 6 0.028869779150355157 1107 7 0.0019168236667823635 1107 8 -0.02686070185520606 1107 9 -0.04539281500168357 1107 10-11 0.04334971306872859 1107 12-13 -0.021087388902365944 1107 14-15 0.07175247970832288 1107 16-17 0.15612682820592028 1107 18-19 0.09684807911348514 1107 20-21 0.06871592082661238 1107 22-23 0.05417535551003283 1107 24-25 0.12195692955836535 1107 26-27 0.11969012725531769 1107 28-29 0.07329017857829001 1107 30-31 0.03808377734424084 1107 32-33 0.07491246939783736 1107 34-35 0.1448574333081467 1107 36-37 0.2121582960392132 1107 38-39 0.18123392354096524 1107 40-41 0.22216761128984786 1107 42-43 0.08990098238615474 1107 44-45 0.177303209266924 1107 46-47 0.35044969993479214 1107 48-49 0.3888581866082603 1107 50-51 0.39885674761409007 1107 52-53 0.27706622648366164 1107 54-55 0.3163270822404556 1107 56-57 0.21654510018348816 1107 58-59 0.21965622925775818 1107 60-61 0.30975749520823115 1107 62-63 0.34588013073948076 1107 64-65 0.15568195899914627 1107 66-67 0.35541997621291443 1107 68-69 0.42689727243713804 1107 70-71 0.6439903877649016 1107 72-73 0.4403533217784812 1107 74-75 0.5632719684803043 1107 76-77 0.6413657317383361 1107 78-79 0.7315580175514889 1107 80-81 0.918994289842658 1107 82-83 1.0286673101828292 1107 84-85 1.1396699866681494 1107 86-87 1.1562779344456864 1107 88-89 1.2217987342187655 1107 90-91 1.4370644498342209 1107 92-93 1.5253069421623238 1107 94-95 1.6408271206645573 1107 96-97 1.797303589000979 1107 98-99 1.816753222470453 1107 100-101 2.1758271618379688 1108 1 0.10866871431768033 1108 2 0.07262775874085037 1108 3 0.14340534788743753 1108 4 0.08411013367307163 1108 5 0.029179019197641765 1108 6 0.0628294160581504 1108 7 -0.0010075138900589309 1108 8 0.01599737159099135 1108 9 0.05507620188393503 1108 10-11 0.03615205656961251 1108 12-13 0.0484138991665688 1108 14-15 0.12432730395295266 1108 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-2.4093343792972917 2316 94-95 -2.55378095283341 2316 96-97 -2.5682556934069716 2316 98-99 -3.0141676755769637 2316 100-101 -3.7504610498211406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 13.0 5 35.0 6 75.0 7 195.0 8 422.0 9 820.0 10 1582.0 11 2482.0 12 3873.0 13 5825.0 14 8068.0 15 11053.0 16 14586.0 17 20468.0 18 20498.0 19 15482.0 20 13714.0 21 13599.0 22 14342.0 23 15602.0 24 17576.0 25 20671.0 26 24118.0 27 28920.0 28 35304.0 29 42763.0 30 51632.0 31 64479.0 32 80889.0 33 105683.0 34 148229.0 35 222564.0 36 350139.0 37 503440.0 38 354566.0 39 29031.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18733800529084 18.728717467850753 15.158714128190345 22.92523039866806 2 15.73783387500134 19.73166920554963 40.16796762121531 24.362529298233724 3 19.89428431668509 23.57966288522482 29.567750919795117 26.958301878294975 4 13.111221392732025 16.72848828421268 36.357353472957 33.8029368500983 5 13.832568790976735 37.05465110472209 34.182935485942174 14.929844618359004 6 32.68623767634129 35.906139769273196 16.835886833019803 14.571735721365705 7 28.951734550057672 32.72189457285119 19.920383209311098 18.405987667780035 8 26.73425372658497 34.78927756576005 19.11423408094663 19.362234626708346 9 26.08877882520593 17.002267759733396 20.00881064339991 36.90014277166076 10-11 25.349159489072086 26.513604099979354 26.586595075762137 21.55064133518642 12-13 26.18318282567374 23.73022678033105 27.269932399744416 22.816657994250793 14-15 22.803861164654176 25.2336536107793 25.745504500515665 26.21698072405086 16-17 22.18901140255482 28.420710710054607 26.783608286816634 22.60666960057394 18-19 22.17324945390533 26.69235842002405 29.216209793097114 21.918182332973508 20-21 22.644034559465798 25.803090233700427 29.6879312520427 21.864943954791073 22-23 22.479459304967175 25.623176618988442 29.65375435348589 22.243609722558492 24-25 22.35856804999679 25.957237334931406 29.57108918519787 22.11310542987394 26-27 21.88372223184778 26.222603676724894 29.54145853722604 22.352215554201287 28-29 21.966470901533995 26.68987044187274 29.37199321276199 21.97166544383128 30-31 22.06036674311328 26.154173363269912 29.269111041107504 22.516348852509307 32-33 21.766796489977207 26.14946468785888 29.380132150900035 22.70360667126388 34-35 22.5093281876492 26.274718526648044 29.055938801202462 22.160014484500294 36-37 22.58465768488062 25.834960740452217 29.148360891305554 22.432020683361607 38-39 22.07586945362773 26.303360970147153 29.08238374989241 22.538385826332703 40-41 22.636185000383545 26.119169011044292 29.072304352247123 22.172341636325037 42-43 22.21756193279372 26.12212634066939 28.93439917943229 22.725912547104603 44-45 22.258958732851248 26.34583051009199 29.040847090573152 22.354363666483614 46-47 22.725253048159914 26.676574342908598 28.261847130619692 22.336325478311796 48-49 22.48553161975632 26.755072098337052 28.17338469025884 22.58601159164779 50-51 22.490227368896036 26.967245020940872 28.193578131778807 22.348949478384284 52-53 22.91738286553564 26.629243743041446 28.05403918326799 22.399334208154926 54-55 22.496536607181476 26.921363705070085 28.03595864528392 22.54614104246452 56-57 22.50815184798258 27.124953683948625 27.90159193451947 22.46530253354932 58-59 22.51002455475282 26.67436705855285 27.91615000024524 22.899458386449094 60-61 22.15788879956375 26.933313357794454 28.234309504031447 22.674488338610356 62-63 22.256518022796193 26.929345002971804 28.388228165242296 22.425908808989707 64-65 22.083939176267634 27.047147176470954 28.10883815042562 22.760075496835793 66-67 22.001088847164116 27.018324468429007 28.035139175802442 22.94544750860443 68-69 22.502484685021308 26.697555979541086 28.181255152739578 22.618704182698032 70-71 22.36451077987982 26.816046243372515 27.739156916329694 23.08028606041798 72-73 22.340856709841475 26.88092215648427 27.779085699966515 22.99913543370774 74-75 22.240064956171974 27.315702244974123 27.553313063511958 22.890919735341946 76-77 22.30895381236281 26.90435328524044 27.82122160229683 22.96547130009992 78-79 22.408229565313547 27.15752540527381 27.71175743275088 22.722487596661768 80-81 22.57495799222335 27.109613042442067 27.481769505553366 22.83365945978122 82-83 22.427080158709103 27.16035152857948 27.64133009054409 22.771238222167327 84-85 22.317736747926226 27.249872806667476 27.59972693007243 22.832663515333877 86-87 22.52093822324013 27.19290364393193 27.392392792975162 22.893765339852774 88-89 22.391296011757035 27.322848548785373 27.15053715496477 23.135318284492822 90-91 22.285978641339486 27.28932289924866 27.321360890018504 23.10333756939335 92-93 22.402882740520806 26.960905183733907 27.421170744366126 23.215041331379165 94-95 22.283370088935776 26.77220563042918 27.566456596434186 23.377967684200858 96-97 22.418943834202437 26.51002013166959 27.64220917763356 23.428826856494414 98-99 22.123835406323085 26.833040907641788 27.255047965987956 23.788075720047175 100-101 22.366676788444586 26.38234726259415 27.54459044477411 23.70638550418716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 427.5 2 363.5 3 519.5 4 425.0 5 394.0 6 747.5 7 1765.0 8 3025.0 9 3537.5 10 3030.0 11 2333.5 12 2201.5 13 2312.5 14 2449.5 15 2567.5 16 2706.0 17 2891.0 18 3171.5 19 3497.0 20 3916.0 21 4641.5 22 5454.5 23 6613.0 24 8336.5 25 10491.5 26 13514.5 27 17061.5 28 19981.0 29 22792.5 30 25997.5 31 30505.5 32 37193.0 33 40345.5 34 40611.5 35 43376.5 36 47866.5 37 52380.5 38 55767.5 39 58667.0 40 61827.0 41 65944.5 42 68753.5 43 70683.0 44 73932.0 45 77820.0 46 79303.5 47 81704.5 48 83987.0 49 81975.5 50 79711.5 51 78522.5 52 77978.5 53 79205.0 54 81577.5 55 77602.5 56 71011.5 57 65454.5 58 60309.5 59 53552.0 60 42867.0 61 35349.0 62 29871.5 63 21729.5 64 14806.5 65 11138.0 66 8286.5 67 6446.0 68 5021.0 69 3777.0 70 2839.5 71 1835.5 72 1202.5 73 874.5 74 608.5 75 404.0 76 267.5 77 166.0 78 94.5 79 43.0 80 30.5 81 17.5 82 7.0 83 4.5 84 2.5 85 2.0 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012796829596614502 3 0.005528943797840412 4 0.0 5 3.1211779503937813E-4 6 1.783530257367875E-4 7 0.0 8 2.6752953860518125E-4 9 4.4588256434196876E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001738942000933678 26-27 0.0199978330107373 28-29 2.0064715395388594E-4 30-31 6.688238465129531E-5 32-33 0.0035447663865186513 34-35 0.015427536726232118 36-37 0.007468532952727976 38-39 0.01979718585678341 40-41 0.022249539960664238 42-43 0.017523184778639372 44-45 0.008761592389319686 46-47 0.0016720596162823828 48-49 3.344119232564766E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.783530257367875E-4 68-69 1.783530257367875E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5605889751968906E-4 82-83 0.0010478240262036267 84-85 0.004191296104814507 86-87 6.465297182958546E-4 88-89 4.4588256434196876E-5 90-91 0.00414670784838031 92-93 7.1341210294715E-4 94-95 4.4588256434196876E-5 96-97 3.56706051473575E-4 98-99 3.56706051473575E-4 100-101 0.002407765847446631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2242743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.22979023436298 #Duplication Level Percentage of deduplicated Percentage of total 1 75.8355267370234 40.367091805241806 2 13.653497341617246 14.535455989194373 3 4.427082578213824 7.069580309655741 4 1.9847205162933284 4.225850270245219 5 1.0751281492130569 2.861442292883496 6 0.6495759657114594 2.0746075437662848 7 0.42606514723289024 1.5875550889365813 8 0.31185055308689963 1.3279791620228596 9 0.21837408359698324 1.0461605996239798 >10 1.2677904599007925 12.33410422435863 >50 0.0960327753199258 3.501234020656885 >100 0.04797512480042122 4.684681006104457 >500 0.0034421485208384257 1.219562435393164 >1k 0.002686554943093249 2.1518193780364085 >5k 2.518645259149921E-4 1.012875873879986 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9480 0.4226966709961864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8106 0.36143240665559984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5079 0.22646375442928593 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2685 0.1197194685258186 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2566 0.11441346601014916 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2531 0.11285287703495228 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2437 0.10866158093013778 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2312 0.10308804887586318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4588256434196876E-5 2 0.0 0.0 0.0 0.0 8.917651286839375E-5 3 0.0 0.0 0.0 0.0 8.917651286839375E-5 4 0.0 0.0 0.0 0.0 8.917651286839375E-5 5 0.0 0.0 0.0 0.0 8.917651286839375E-5 6 0.0 0.0 0.0 0.0 8.917651286839375E-5 7 0.0 0.0 0.0 0.0 8.917651286839375E-5 8 0.0 0.0 0.0 0.0 8.917651286839375E-5 9 0.0 0.0 0.0 0.0 8.917651286839375E-5 10-11 0.0 0.0 0.0 0.0 8.917651286839375E-5 12-13 0.0 0.0 0.0 6.688238465129531E-5 1.3376476930259063E-4 14-15 0.0 0.0 0.0 8.917651286839375E-5 1.5605889751968906E-4 16-17 0.0 0.0 0.0 8.917651286839375E-5 2.2294128217098438E-4 18-19 0.0 0.0 0.0 8.917651286839375E-5 2.6752953860518125E-4 20-21 0.0 0.0 0.0 1.1147064108549219E-4 2.898236668222797E-4 22-23 0.0 0.0 0.0 2.6752953860518125E-4 3.1211779503937813E-4 24-25 0.0 0.0 0.0 4.904708207761656E-4 3.1211779503937813E-4 26-27 0.0 0.0 0.0 8.025886158155438E-4 3.3441192325647654E-4 28-29 0.0 0.0 0.0 0.003723119412255439 4.012943079077719E-4 30-31 0.0 0.0 0.0 0.01199424098079896 4.235884361248703E-4 32-33 0.0 0.0 0.0 0.0313232501450233 4.4588256434196876E-4 34-35 0.0 0.0 0.0 0.058410615928797906 4.904708207761656E-4 36-37 0.0 0.0 0.0 0.10063569477198234 4.904708207761656E-4 38-39 0.0 0.0 0.0 0.16678237319211342 5.350590772103625E-4 40-41 0.0 0.0 0.0 0.23435587581813877 5.350590772103625E-4 42-43 0.0 2.2294128217098438E-5 0.0 0.30487220336882115 5.350590772103625E-4 44-45 0.0 4.4588256434196876E-5 0.0 0.38896565500371644 5.796473336445594E-4 46-47 0.0 4.4588256434196876E-5 0.0 0.4866585248510418 6.242355900787563E-4 48-49 0.0 4.4588256434196876E-5 0.0 0.5882082788799252 6.688238465129531E-4 50-51 0.0 4.4588256434196876E-5 0.0 0.6989878019906872 6.688238465129531E-4 52-53 0.0 4.4588256434196876E-5 0.0 0.7995343202498013 8.025886158155438E-4 54-55 0.0 4.4588256434196876E-5 0.0 0.9094220782318794 8.025886158155438E-4 56-57 0.0 4.4588256434196876E-5 0.0 1.0401102578405106 8.025886158155438E-4 58-59 0.0 4.4588256434196876E-5 0.0 1.1766172049138042 8.248827440326422E-4 60-61 0.0 8.917651286839375E-5 0.0 1.3209716851195166 8.471768722497406E-4 62-63 0.0 8.917651286839375E-5 0.0 1.4583035149368428 8.471768722497406E-4 64-65 0.0 8.917651286839375E-5 0.0 1.6016101711163517 8.69471000466839E-4 66-67 0.0 8.917651286839375E-5 0.0 1.7684594266931164 8.917651286839375E-4 68-69 0.0 8.917651286839375E-5 0.0 1.9522076314584416 8.917651286839375E-4 70-71 0.0 8.917651286839375E-5 0.0 2.141261838739436 9.363533851181343E-4 72-73 0.0 8.917651286839375E-5 0.0 2.3337493417658646 0.0010478240262036267 74-75 0.0 8.917651286839375E-5 0.0 2.5308740234614486 0.001070118154420725 76-77 0.0 8.917651286839375E-5 0.0 2.739948357881398 0.001070118154420725 78-79 0.0 8.917651286839375E-5 0.0 2.9767119995469833 0.001070118154420725 80-81 0.0 8.917651286839375E-5 0.0 3.2233742341409606 0.001070118154420725 82-83 0.0 8.917651286839375E-5 0.0 3.4725111169670355 0.001070118154420725 84-85 0.0 8.917651286839375E-5 0.0 3.727399884873122 0.001070118154420725 86-87 0.0 8.917651286839375E-5 0.0 3.9864353606275884 0.0011592946672891189 88-89 0.0 8.917651286839375E-5 0.0 4.269570788984739 0.0012484711801575125 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5655 0.0 44.018307 1 GTATCAA 9140 0.0 38.09715 1 ATCAACG 11515 0.0 30.028547 3 TATCAAC 11625 0.0 29.871643 2 TCAACGC 11805 0.0 29.530962 4 CAACGCA 11870 0.0 29.329239 5 AACGCAG 12275 0.0 28.400246 6 GTAAGGT 1300 0.0 27.40094 4 TAAGGTG 1405 0.0 26.70535 5 ACGCAGA 13750 0.0 25.215508 7 CGCAGAG 13830 0.0 24.966621 8 AGGTAAG 1460 0.0 23.101501 2 GTACATG 8100 0.0 22.75524 1 GAGTACT 9015 0.0 22.65428 12-13 GCAGAGT 15350 0.0 22.308708 9 TACATGG 8010 0.0 22.239931 2 GGTAAGG 1555 0.0 21.992216 3 AAGGTAA 1555 0.0 21.384659 1 GTACTTT 9510 0.0 21.37523 14-15 AGAGTAC 12850 0.0 21.32653 10-11 >>END_MODULE