FastQCFastQC Report
Thu 26 May 2016
SRR938229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265215
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100110.44194480435631944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82940.36614625984729926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51180.2259388181695777No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27780.12263736554808263No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24590.10855481709241728No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24490.1081133578931801No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24040.1061267914966129No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.100520259666301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59300.044.0253261
GTATCAA96950.037.2172241
TATCAAC119750.029.8035682
ATCAACG120150.029.7043463
TCAACGC121400.029.3984934
CAACGCA122950.028.9892225
AACGCAG126650.028.1798426
ACGCAGA140650.025.2846957
CGCAGAG142850.024.8287248
TAAGGTG15100.024.5483045
GTAAGGT13350.022.7825584
GAGTACT88500.022.72191612-13
TACATGG82900.022.2423692
GTACATG84550.022.1280141
GCAGAGT158200.021.9077459
ACATGGG81850.021.6567823
AGGTAAG13000.021.5681292
TGGTATC31100.021.5457972
GTACTTT94950.021.18751114-15
AGAGTAC130300.021.14392710-11
Produced by FastQC (version 0.11.5)