##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2265215 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01754049836329 33.0 31.0 34.0 30.0 34.0 2 32.204077758623356 34.0 31.0 34.0 30.0 34.0 3 32.27859518853619 34.0 31.0 34.0 30.0 34.0 4 35.78584814245006 37.0 35.0 37.0 35.0 37.0 5 35.809667956463294 37.0 35.0 37.0 35.0 37.0 6 35.837728427544405 37.0 36.0 37.0 35.0 37.0 7 35.78601236527217 37.0 36.0 37.0 35.0 37.0 8 35.7684612718881 37.0 36.0 37.0 35.0 37.0 9 37.45422973095269 39.0 38.0 39.0 35.0 39.0 10-11 37.37932867299572 39.0 37.5 39.0 35.0 39.0 12-13 37.29069094986569 39.0 37.0 39.0 34.0 39.0 14-15 38.579853347254016 40.0 38.0 41.0 34.0 41.0 16-17 38.51461870065314 40.0 38.0 41.0 34.0 41.0 18-19 38.46266645770931 40.0 38.0 41.0 34.0 41.0 20-21 38.358018775259744 40.0 38.0 41.0 34.0 41.0 22-23 38.21809894425033 40.0 38.0 41.0 34.0 41.0 24-25 38.17657242248528 40.0 38.0 41.0 33.5 41.0 26-27 38.189063510527696 40.0 38.0 41.0 34.0 41.0 28-29 38.106513951214346 40.0 38.0 41.0 33.0 41.0 30-31 37.91971689221553 40.0 38.0 41.0 33.0 41.0 32-33 37.69669700227131 40.0 37.5 41.0 33.0 41.0 34-35 37.496700975404096 40.0 37.0 41.0 32.5 41.0 36-37 37.284612277421786 40.0 37.0 41.0 31.5 41.0 38-39 37.04944828636576 40.0 36.5 41.0 31.0 41.0 40-41 36.82479588030276 40.0 36.0 41.0 30.5 41.0 42-43 36.54807424460813 39.0 36.0 41.0 30.0 41.0 44-45 36.32041263191353 39.0 35.5 41.0 30.0 41.0 46-47 36.17686842087837 39.0 35.5 41.0 29.5 41.0 48-49 35.916663760393604 39.0 35.0 41.0 28.0 41.0 50-51 35.263655547045204 38.5 34.5 40.5 26.5 40.5 52-53 35.32238440942692 39.0 35.0 40.0 27.0 41.0 54-55 35.463678944382764 39.0 35.0 41.0 27.0 41.0 56-57 35.25955328743629 39.0 35.0 41.0 26.5 41.0 58-59 34.99737883600453 38.5 35.0 40.0 26.0 41.0 60-61 34.636258147681346 38.0 34.0 40.0 26.0 41.0 62-63 34.230824667857135 37.5 34.0 40.0 24.5 41.0 64-65 33.81497606187492 37.0 34.0 40.0 23.0 41.0 66-67 33.3903384446951 36.0 33.0 39.0 22.0 41.0 68-69 32.918445931180926 36.0 33.0 39.0 20.5 41.0 70-71 32.44737695980293 35.0 32.0 38.5 19.5 40.0 72-73 31.971390795134237 35.0 32.0 37.5 17.5 40.0 74-75 31.463828599051304 35.0 31.0 37.0 13.0 39.0 76-77 30.947405433921283 35.0 31.0 36.5 7.5 39.0 78-79 30.403880867820494 34.5 30.5 36.0 6.5 38.5 80-81 29.90047699666478 34.0 30.0 35.5 2.0 37.0 82-83 29.390354557955867 34.0 29.0 35.0 2.0 37.0 84-85 28.922902461797225 34.0 29.0 35.0 2.0 36.5 86-87 28.45240208986785 34.0 28.5 35.0 2.0 36.0 88-89 27.928673437179253 34.0 27.0 35.0 2.0 35.5 90-91 27.359539160741917 33.0 26.0 35.0 2.0 35.0 92-93 26.784038380462782 33.0 25.0 35.0 2.0 35.0 94-95 26.220702891337027 33.0 24.5 35.0 2.0 35.0 96-97 25.423295360484545 32.0 21.0 34.5 2.0 35.0 98-99 24.408428118302236 32.0 9.5 34.0 2.0 35.0 100-101 21.513793171950567 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27166858772412894 1101 2 0.17893306667180298 1101 3 0.2404940083370235 1101 4 0.11789243318028753 1101 5 0.18376982480118187 1101 6 0.11497367376482259 1101 7 0.10522025471247076 1101 8 0.12510846150144062 1101 9 0.19162731958704882 1101 10-11 0.1304305614285184 1101 12-13 0.045476373742701526 1101 14-15 0.19859003072086523 1101 16-17 0.19855495894194775 1101 18-19 0.18531029992946912 1101 20-21 0.24683554211163283 1101 22-23 0.36508532991006604 1101 24-25 0.2365894687253416 1101 26-27 0.1608547118400523 1101 28-29 0.1402664355586154 1101 30-31 0.16857882537853897 1101 32-33 0.10300757249822823 1101 34-35 0.1589303035981331 1101 36-37 0.1925034434235755 1101 38-39 0.15269750345288458 1101 40-41 0.16765794140806634 1101 42-43 0.3213261521848949 1101 44-45 0.3150008125116983 1101 46-47 0.32182780300200164 1101 48-49 0.32635617350616997 1101 50-51 0.4691511487030695 1101 52-53 0.44500539730751143 1101 54-55 0.3772256122386821 1101 56-57 0.26979779878042365 1101 58-59 0.2826255399238349 1101 60-61 0.31288754540474173 1101 62-63 0.21653505113695815 1101 64-65 0.2337438780795793 1101 66-67 0.09647223012071748 1101 68-69 0.065765562319136 1101 70-71 0.291646928052991 1101 72-73 0.11636068670711808 1101 74-75 0.160316722551741 1101 76-77 0.16527806477857965 1101 78-79 0.21879073833753893 1101 80-81 0.25354133967184467 1101 82-83 0.35545725999344313 1101 84-85 0.33530502934163664 1101 86-87 0.3509880410825126 1101 88-89 0.28489393435628685 1101 90-91 0.2954793158740614 1101 92-93 0.5508493401320926 1101 94-95 0.6419191163960392 1101 96-97 0.6183276616253046 1101 98-99 0.5864700693186116 1101 100-101 0.5202426437533596 1102 1 0.3460430940104473 1102 2 0.23623496685647183 1102 3 0.16171496953494824 1102 4 0.11853788637385776 1102 5 0.10549069582550175 1102 6 0.1985266404765227 1102 7 0.1110767932482375 1102 8 0.20757143495243469 1102 9 0.2358173971152553 1102 10-11 0.32411951626894364 1102 12-13 0.14528115579150835 1102 14-15 0.26433160054318705 1102 16-17 0.14329198422536393 1102 18-19 0.2197687467708107 1102 20-21 0.2718425028813627 1102 22-23 0.22129982075613697 1102 24-25 0.046037474412074175 1102 26-27 0.12599522974240784 1102 28-29 0.07132330715666768 1102 30-31 0.06577379337481659 1102 32-33 0.22480034510991231 1102 34-35 0.16840050992167477 1102 36-37 0.1822920733226141 1102 38-39 0.21547665932244797 1102 40-41 0.4067851663495574 1102 42-43 0.483783662486303 1102 44-45 0.39037026063866875 1102 46-47 0.3116120824199484 1102 48-49 0.2846325550871285 1102 50-51 0.2232245173253915 1102 52-53 0.08691835393664604 1102 54-55 0.32119866667718355 1102 56-57 0.40405219487563926 1102 58-59 0.22811190261579384 1102 60-61 0.18192303583843028 1102 62-63 0.20738651674812303 1102 64-65 0.07556486817524899 1102 66-67 0.163663301140609 1102 68-69 0.03394508900788651 1102 70-71 0.1001771140637473 1102 72-73 0.3499767749443734 1102 74-75 0.13418338267950247 1102 76-77 0.22386151122442044 1102 78-79 0.27569700840907885 1102 80-81 0.43142499673369983 1102 82-83 0.5616169347778452 1102 84-85 0.3766189115422094 1102 86-87 0.4301120862178287 1102 88-89 0.5620429138125225 1102 90-91 0.6377313990953368 1102 92-93 0.7312161990636596 1102 94-95 0.8634146325001986 1102 96-97 0.8200176785104496 1102 98-99 1.1511321925010343 1102 100-101 0.9376052555607259 1103 1 0.3970100967131174 1103 2 0.3298112326434861 1103 3 0.1926169230653798 1103 4 0.18562485495911574 1103 5 0.16500503644475373 1103 6 0.1482846328925902 1103 7 0.19635903266618726 1103 8 0.24666259628055087 1103 9 0.2736913270923438 1103 10-11 0.2666450272594787 1103 12-13 0.180981128045417 1103 14-15 0.3145556245486958 1103 16-17 0.33383981540580265 1103 18-19 0.3550098808666462 1103 20-21 0.2896701379894253 1103 22-23 0.34913891397272323 1103 24-25 0.4656552462980912 1103 26-27 0.32941981689082667 1103 28-29 0.39475976346685115 1103 30-31 0.35488036919871746 1103 32-33 0.41716837133510865 1103 34-35 0.5705238567170099 1103 36-37 0.5030962475680809 1103 38-39 0.40590759724314296 1103 40-41 0.4519285725420801 1103 42-43 0.3511254840686462 1103 44-45 0.4068439891996718 1103 46-47 0.2412017485419753 1103 48-49 0.17819828763534673 1103 50-51 0.06759462964568286 1103 52-53 0.2227668191666723 1103 54-55 0.22030796572867217 1103 56-57 0.135277595909443 1103 58-59 0.20202954810294926 1103 60-61 0.26916908202026235 1103 62-63 0.17875434772303578 1103 64-65 0.2673145162109307 1103 66-67 0.33203866466580223 1103 68-69 0.4437944380023495 1103 70-71 0.5898655871037946 1103 72-73 0.5421693328258002 1103 74-75 0.3377788138229505 1103 76-77 0.5201864385193815 1103 78-79 0.5167434985283847 1103 80-81 0.4323460128572627 1103 82-83 0.4400024718702582 1103 84-85 0.727480838765235 1103 86-87 0.7147065822152001 1103 88-89 0.6762558496573909 1103 90-91 0.753687971322524 1103 92-93 0.7932577462679866 1103 94-95 0.7206807689082133 1103 96-97 0.5042658621690244 1103 98-99 0.6572928526525068 1103 100-101 0.8481742046004008 1104 1 0.3477067077302891 1104 2 0.2701265533227115 1104 3 0.2853031027791175 1104 4 0.2741480396027143 1104 5 0.2017098877153245 1104 6 0.25777997691126586 1104 7 0.2567081717264159 1104 8 0.2426686931154336 1104 9 0.19763569347401244 1104 10-11 0.2967898270880056 1104 12-13 0.25154841947889395 1104 14-15 0.2066880878128856 1104 16-17 0.34159541806364047 1104 18-19 0.46081973582866453 1104 20-21 0.27336824939954596 1104 22-23 0.29751348826599866 1104 24-25 0.49931406170212256 1104 26-27 0.4249567432286838 1104 28-29 0.43253513320654235 1104 30-31 0.6024702141581173 1104 32-33 0.5423094797206431 1104 34-35 0.3921211798254376 1104 36-37 0.5720000080767846 1104 38-39 0.5070638524528306 1104 40-41 0.5528633411719497 1104 42-43 0.6453751685098155 1104 44-45 0.7640744543214879 1104 46-47 0.6932129895812906 1104 48-49 0.7195245873805263 1104 50-51 0.7046388555143679 1104 52-53 0.772255971087418 1104 54-55 0.8569532775960695 1104 56-57 0.7700033574184104 1104 58-59 0.7088730071041098 1104 60-61 0.8447948411476531 1104 62-63 0.7769765011441692 1104 64-65 0.7149793808183205 1104 66-67 0.6461042532723411 1104 68-69 0.7989636489861809 1104 70-71 0.844516805279973 1104 72-73 0.9172520925962218 1104 74-75 0.7452212392200792 1104 76-77 0.8021126572909658 1104 78-79 0.7438613150993909 1104 80-81 0.8311218827279454 1104 82-83 0.9417957986939527 1104 84-85 0.8792694024366732 1104 86-87 0.7733909995625972 1104 88-89 0.8324038884294502 1104 90-91 0.8455798953978793 1104 92-93 0.9323906144001128 1104 94-95 0.971301169489319 1104 96-97 0.8969579958230796 1104 98-99 1.020437962085932 1104 100-101 1.1346635232314704 1105 1 0.3670420747852958 1105 2 0.3517390535662841 1105 3 0.27717608249891157 1105 4 0.279411972364322 1105 5 0.25883783681021555 1105 6 0.2777501422575952 1105 7 0.23697118433361197 1105 8 0.2818384756775316 1105 9 0.3953450502536029 1105 10-11 0.3113458038697985 1105 12-13 0.26458094531358256 1105 14-15 0.41350491646280574 1105 16-17 0.4234469419476099 1105 18-19 0.42008525775106875 1105 20-21 0.49556323986242035 1105 22-23 0.4228712118256297 1105 24-25 0.4704747986050819 1105 26-27 0.3904979347528652 1105 28-29 0.4838643774458262 1105 30-31 0.5259630553567831 1105 32-33 0.4054735335096211 1105 34-35 0.5853251358398879 1105 36-37 0.5406247994182394 1105 38-39 0.546337017069547 1105 40-41 0.47657452960009294 1105 42-43 0.4970368591257568 1105 44-45 0.5668257160155719 1105 46-47 0.586130939953577 1105 48-49 0.6136254233137421 1105 50-51 0.48216749396728886 1105 52-53 0.5602725295367108 1105 54-55 0.5680009762357585 1105 56-57 0.6911935392625708 1105 58-59 0.6212392328905239 1105 60-61 0.6676387028516899 1105 62-63 0.519731963072509 1105 64-65 0.6531527985316217 1105 66-67 0.5188362891518636 1105 68-69 0.5294956714422696 1105 70-71 0.6145115441618074 1105 72-73 0.7271624803817645 1105 74-75 0.6498847982907421 1105 76-77 0.7722650132110225 1105 78-79 0.7546831970208494 1105 80-81 0.8511445510025339 1105 82-83 0.8727114714134316 1105 84-85 0.8286000712915076 1105 86-87 1.033939108482901 1105 88-89 1.040257676975802 1105 90-91 0.885186372418918 1105 92-93 0.9375930591962636 1105 94-95 1.193065419433566 1105 96-97 1.2628314172169937 1105 98-99 1.2309566260650229 1105 100-101 0.9518745243811253 1106 1 0.04425436815979822 1106 2 0.04348919310770327 1106 3 -0.02911202579340255 1106 4 0.034286319706829715 1106 5 -0.007639492101375822 1106 6 0.028884861225670022 1106 7 0.14029472184553526 1106 8 -0.01859931254803371 1106 9 0.002882452666547408 1106 10-11 -0.3627528566142786 1106 12-13 -0.19792592960418887 1106 14-15 -0.10932900432015913 1106 16-17 0.09523152349904507 1106 18-19 0.07656870360287371 1106 20-21 0.10185089868017627 1106 22-23 0.06266495203423261 1106 24-25 0.0705037284224872 1106 26-27 0.02230665717453917 1106 28-29 -0.10247978849690753 1106 30-31 0.03800866721833529 1106 32-33 0.01742457183672741 1106 34-35 -0.018722913808254305 1106 36-37 -0.16808299389865056 1106 38-39 -0.058145899219496755 1106 40-41 0.2643026072954484 1106 42-43 0.04455214330400281 1106 44-45 0.3242748375379705 1106 46-47 0.3776617767689814 1106 48-49 0.553861037880317 1106 50-51 0.625939799142543 1106 52-53 0.5712754625625465 1106 54-55 0.0969892253361948 1106 56-57 0.25797718508969325 1106 58-59 0.44213526452045215 1106 60-61 0.20469869789930328 1106 62-63 0.3053576234936557 1106 64-65 0.2736984034467511 1106 66-67 0.0825935410153491 1106 68-69 -0.210371741733546 1106 70-71 0.1350663224441746 1106 72-73 0.11748036922425342 1106 74-75 0.2831018062448081 1106 76-77 0.46901106253390523 1106 78-79 0.5082565198065119 1106 80-81 0.22930711532847425 1106 82-83 0.41460311371993797 1106 84-85 0.41204543275486216 1106 86-87 0.3685927392975721 1106 88-89 0.18447067153062235 1106 90-91 0.2215339094208879 1106 92-93 0.27185534068011563 1106 94-95 0.19917296153503017 1106 96-97 0.3121660518472922 1106 98-99 0.2518072403333065 1106 100-101 4.146105658300314E-4 1107 1 0.1236063414996309 1107 2 0.04754556856916281 1107 3 0.07261945602063236 1107 4 0.003349316652460743 1107 5 0.10786414188223858 1107 6 0.13255541010200744 1107 7 -0.0590618135555232 1107 8 0.15017632039020157 1107 9 0.13547203756482418 1107 10-11 0.10149494966088923 1107 12-13 -0.026521571269178423 1107 14-15 0.09866755541190031 1107 16-17 0.15420638554827093 1107 18-19 0.2597277246777594 1107 20-21 0.13164528497595285 1107 22-23 0.24907730734926048 1107 24-25 0.23379227917713763 1107 26-27 0.28042580797396965 1107 28-29 0.26339608905033884 1107 30-31 0.24596329343115997 1107 32-33 0.1687818423638987 1107 34-35 0.34978732484791664 1107 36-37 0.21249683873407577 1107 38-39 0.21552896336464755 1107 40-41 0.2511091185871237 1107 42-43 0.18845909788742432 1107 44-45 0.25892627160700954 1107 46-47 0.19401053832600468 1107 48-49 0.18501934293319522 1107 50-51 0.09858147330438527 1107 52-53 0.17220495388125556 1107 54-55 0.18850134612917913 1107 56-57 0.19116982939640081 1107 58-59 0.1631887185815657 1107 60-61 0.05597206317980152 1107 62-63 0.24181076608137886 1107 64-65 0.2724514782530889 1107 66-67 0.3480400728171915 1107 68-69 0.3201359919918545 1107 70-71 0.4026725737671413 1107 72-73 0.4176304702085716 1107 74-75 0.37274583174038867 1107 76-77 0.4904248582987556 1107 78-79 0.46314541412945687 1107 80-81 0.40084023535806423 1107 82-83 0.4412382535123456 1107 84-85 0.49781982545433934 1107 86-87 0.49377986404510565 1107 88-89 0.5214210176588701 1107 90-91 0.6291456741897008 1107 92-93 0.6639137816778415 1107 94-95 0.6810572991459019 1107 96-97 0.6798468856117417 1107 98-99 0.7018959407113385 1107 100-101 0.6709381739559355 1108 1 0.4092801351506239 1108 2 0.32312961330317336 1108 3 0.3147112105162648 1108 4 0.26668903445968795 1108 5 0.2537263260272482 1108 6 0.21903489185349656 1108 7 0.21595916278869254 1108 8 0.12946031571246408 1108 9 0.25328777611770903 1108 10-11 0.3193131806328182 1108 12-13 0.10845174347349484 1108 14-15 0.1714835777730812 1108 16-17 0.3908437447629822 1108 18-19 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sequence quality scores pass #Quality Count 2 5116.0 3 1347.0 4 1470.0 5 1557.0 6 1893.0 7 2264.0 8 2880.0 9 3508.0 10 4736.0 11 5818.0 12 7444.0 13 9385.0 14 11264.0 15 13881.0 16 17091.0 17 22987.0 18 20893.0 19 16207.0 20 15336.0 21 15798.0 22 16680.0 23 18430.0 24 20145.0 25 22568.0 26 25784.0 27 30443.0 28 35197.0 29 41823.0 30 49988.0 31 62135.0 32 78153.0 33 102573.0 34 142263.0 35 212651.0 36 329639.0 37 470010.0 38 375986.0 39 49868.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89345087915316 18.450368868935048 14.875805694416957 22.780374557494834 2 15.525983599533937 19.462015951719064 40.739248843460615 24.27275160528638 3 19.581963443997864 23.69456889952555 29.50599173362063 27.21747592285596 4 12.892351622681582 16.429137500099394 36.70145892472662 33.977051952492396 5 13.82126860165399 37.04571140253445 34.14879732646924 14.984222669342318 6 32.61076794779498 35.846932809373996 17.011310468447757 14.530988774383266 7 28.78953878777169 32.8028361901396 20.066266124757025 18.341358897331684 8 26.62214102916325 34.78406052259394 19.250985197088003 19.34281325115481 9 26.27388485799052 17.07068650319186 20.083090441463245 36.57233819735438 10-11 25.238044852388448 26.545493985729568 26.613861814811322 21.602599347070665 12-13 26.272190159764293 23.814561880101877 27.248426818268396 22.66482114186544 14-15 22.89287357296652 25.24781700401317 25.806185160817407 26.053124262202903 16-17 22.160448481480614 28.45282988863499 26.7281984365344 22.658523193349993 18-19 22.17479082688397 26.743503171250627 29.246402540445953 21.83530346141945 20-21 22.722232285256087 25.84437444499914 29.668302768708777 21.765090501036003 22-23 22.51623361205295 25.683574272626615 29.5932911156714 22.206900999649033 24-25 22.326629406756183 25.955737029101698 29.61000812565919 22.107625438482927 26-27 21.772942919322993 26.242904590472367 29.53797533639072 22.446177153813924 28-29 21.992197258327824 26.65880045933821 29.343198929712816 22.00580335262115 30-31 22.055506653663794 26.219466536196173 29.2151117521738 22.50991505796623 32-33 21.773759614067796 26.196959723627305 29.279491608536805 22.749789053768097 34-35 22.54367078695442 26.374524503596223 29.017689206206782 22.064115503242576 36-37 22.60407771630923 25.886867735521403 29.109379507339632 22.399675040829734 38-39 22.059151512153715 26.319764234834185 28.9924377960921 22.62864645691999 40-41 22.62526474646944 26.127150295612857 29.02745325103475 22.220131706882956 42-43 22.259941450669306 26.122068531026578 28.874642292384944 22.743347725919175 44-45 22.25002307129494 26.471650566803213 28.94213365984919 22.33619270205266 46-47 22.747951880648817 26.71617553384682 28.22488963173042 22.310982953773937 48-49 22.454560709365172 26.80156410075246 28.14683647154414 22.59703871833822 50-51 22.558995635202844 27.00491103157275 28.15542554950763 22.280667783716783 52-53 22.884721616899174 26.699669835836392 28.01529255759548 22.400315989668957 54-55 22.47488725011718 26.99322827305839 28.06684428163195 22.465040195192472 56-57 22.487640441499856 27.16210583208663 27.92105514155867 22.429198584854838 58-59 22.53590749889169 26.688363356335564 27.913112860465894 22.86261628430685 60-61 22.176542501974446 26.905431277032022 28.271657247248307 22.646368973745226 62-63 22.281573932287387 26.863871830331675 28.479371656360886 22.375182581020052 64-65 22.098650027619392 26.981807407826068 28.207569789196246 22.71197277535829 66-67 22.040020222045122 27.035549737179643 28.053407392526232 22.871022648249003 68-69 22.52620758900778 26.661889207091832 28.261234315405936 22.550668888494446 70-71 22.40570230207989 26.751656211901498 27.864505183439224 22.978136302579387 72-73 22.339272840088192 26.795923346909667 27.90765150820928 22.95715230479286 74-75 22.26496699344594 27.277321957036833 27.736479258212004 22.721231791305225 76-77 22.435797660215247 26.8613815852948 27.852728987186136 22.850091767303816 78-79 22.53456203903392 27.093066663899773 27.75056388284306 22.621807414223248 80-81 22.659941483221296 27.10459835558712 27.48654915477849 22.748911006413092 82-83 22.542028054395548 27.155029954199385 27.667546471233038 22.63539552017203 84-85 22.50092778796143 27.277937456163258 27.584013655979096 22.637121099896216 86-87 22.706507229654243 27.136131538404996 27.40873501083769 22.74862622110307 88-89 22.552068680885533 27.208980028433018 27.227352403800076 23.011598886881373 90-91 22.502915726504522 27.285104717701802 27.312754705080422 22.899224850713257 92-93 22.652443871332427 26.900072424087192 27.484300754270368 22.96318295031001 94-95 22.511016494540726 26.74460129377694 27.62333692507238 23.121045286609952 96-97 22.748214119699966 26.40475006950519 27.66549356190952 23.18154224888532 98-99 22.48202201342851 26.686203540307606 27.347560275494647 23.48421417076923 100-101 22.9508486684123 26.059953892505906 27.611366932211105 23.37783050687069 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 686.0 1 484.5 2 393.5 3 542.0 4 465.0 5 409.5 6 774.0 7 1789.0 8 3059.0 9 3589.0 10 3048.0 11 2403.0 12 2350.5 13 2490.5 14 2581.0 15 2734.5 16 2906.5 17 3055.0 18 3259.0 19 3567.5 20 4080.0 21 4836.5 22 5845.5 23 7135.0 24 8682.0 25 10793.0 26 13832.5 27 17582.5 28 20674.5 29 23239.0 30 26434.5 31 31181.0 32 37503.0 33 40657.0 34 41194.5 35 44065.5 36 48587.0 37 52718.5 38 56201.5 39 59304.5 40 62534.5 41 66643.5 42 69430.5 43 71296.5 44 74335.0 45 78414.0 46 79980.0 47 81968.5 48 84364.0 49 82706.5 50 80406.0 51 79030.0 52 78228.0 53 79017.0 54 81231.5 55 77530.5 56 70601.5 57 65000.5 58 60465.0 59 53865.5 60 43114.5 61 35538.5 62 30127.5 63 22281.0 64 15460.0 65 11774.0 66 8897.0 67 6909.5 68 5423.5 69 4156.0 70 3140.5 71 2025.5 72 1386.0 73 1012.5 74 683.5 75 486.5 76 346.0 77 213.5 78 125.0 79 78.5 80 55.0 81 32.0 82 15.0 83 17.0 84 14.0 85 10.5 86 11.0 87 8.0 88 5.5 89 3.0 90 1.5 91 5.5 92 7.0 93 2.0 94 1.5 95 3.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5107241475974686 2 0.050017327273570056 3 0.05942040821732154 4 0.06590985844610776 5 0.08127263857956088 6 0.10789262829356153 7 0.0712956606768011 8 0.053019249828382735 9 0.051915601830289836 10-11 0.07608549298852427 12-13 0.10789262829356153 14-15 0.0572351851810976 16-17 0.06171599605335476 18-19 0.06006052405621541 20-21 0.07526879346993552 22-23 0.06491657524782415 24-25 0.08890988272636371 26-27 0.06690314164439137 28-29 0.0672563090037811 30-31 0.07118529587699181 32-33 0.07085420147756394 34-35 0.07326015411340646 36-37 0.06824959220206471 38-39 0.04096741368920831 40-41 0.04505091128215203 42-43 0.04284361528596624 44-45 0.021896376282163062 46-47 0.028143023951368855 48-49 0.028915577550033884 50-51 0.02734839739274197 52-53 0.025781217235450057 54-55 0.025538414675869616 56-57 0.025207320276441753 58-59 0.022381981401323935 60-61 0.02893765050999574 62-63 0.020616144604375303 64-65 0.022161251801705355 66-67 0.015892531172537708 68-69 0.018232264928494644 70-71 0.041342654008559895 72-73 0.03785512633458634 74-75 0.03269005370351159 76-77 0.022933805400370384 78-79 0.014943393894177814 80-81 0.017415565409905902 82-83 0.022404054361285793 84-85 0.018519213407998798 86-87 0.008983694704476176 88-89 0.04176204024783519 90-91 0.05295303094849717 92-93 0.03421308794087979 94-95 0.054012533026666344 96-97 0.04394726328405913 98-99 0.034389671620574644 100-101 0.16601073187313345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2265215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.671734297876505 #Duplication Level Percentage of deduplicated Percentage of total 1 77.13499724231427 42.17104074299243 2 12.90446815527411 14.110193084811092 3 4.261765554637282 6.989943421289155 4 1.8599010688308555 4.067360682218283 5 0.9944739101092473 2.7184806689831547 6 0.6089785250931992 1.9976347270204864 7 0.4083047358696701 1.5625909622421874 8 0.2734713725970328 1.196092337656045 9 0.2150718879519609 1.0582517801747047 >10 1.2003057600202567 12.009439020157993 >50 0.08747866848520368 3.2981796496163485 >100 0.04454118163983843 4.411169475172093 >500 0.003404693005691245 1.2206383006082848 >1k 0.00259405181385976 2.1508974658745577 >5k 1.62128238366235E-4 0.5944085719176949 >10k+ 8.10641191831175E-5 0.4436791092654372 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10011 0.44194480435631944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8294 0.36614625984729926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5118 0.2259388181695777 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2778 0.12263736554808263 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2459 0.10855481709241728 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2449 0.1081133578931801 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2404 0.1061267914966129 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2277 0.100520259666301 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.414591992371585E-5 0.0 0.0 0.0 0.0 2 4.414591992371585E-5 0.0 0.0 0.0 0.0 3 4.414591992371585E-5 0.0 0.0 0.0 0.0 4 4.414591992371585E-5 0.0 0.0 0.0 0.0 5 4.414591992371585E-5 0.0 0.0 0.0 0.0 6 4.414591992371585E-5 0.0 0.0 0.0 0.0 7 4.414591992371585E-5 0.0 0.0 0.0 0.0 8 4.414591992371585E-5 0.0 0.0 0.0 0.0 9 4.414591992371585E-5 0.0 0.0 0.0 0.0 10-11 4.414591992371585E-5 0.0 0.0 2.2072959961857924E-5 0.0 12-13 4.414591992371585E-5 0.0 0.0 4.414591992371585E-5 8.82918398474317E-5 14-15 4.414591992371585E-5 0.0 0.0 4.414591992371585E-5 8.82918398474317E-5 16-17 4.414591992371585E-5 0.0 0.0 4.414591992371585E-5 8.82918398474317E-5 18-19 4.414591992371585E-5 0.0 0.0 4.414591992371585E-5 8.82918398474317E-5 20-21 4.414591992371585E-5 0.0 0.0 1.3243775977114756E-4 1.3243775977114756E-4 22-23 4.414591992371585E-5 0.0 0.0 2.2072959961857926E-4 1.3243775977114756E-4 24-25 4.414591992371585E-5 0.0 0.0 5.738969590083061E-4 1.3243775977114756E-4 26-27 4.414591992371585E-5 0.0 0.0 0.0010595020781691805 1.3243775977114756E-4 28-29 4.414591992371585E-5 0.0 0.0 0.0030681414346982517 1.3243775977114756E-4 30-31 4.414591992371585E-5 0.0 0.0 0.010881969261195957 1.5451071973300546E-4 32-33 4.414591992371585E-5 0.0 0.0 0.02887143163011017 1.765836796948634E-4 34-35 4.414591992371585E-5 0.0 0.0 0.056021172383195414 2.2072959961857926E-4 36-37 4.414591992371585E-5 0.0 0.0 0.10058647854618656 2.2072959961857926E-4 38-39 4.414591992371585E-5 0.0 0.0 0.16428904099610853 2.2072959961857926E-4 40-41 4.414591992371585E-5 0.0 0.0 0.23161156887977521 2.2072959961857926E-4 42-43 4.414591992371585E-5 0.0 0.0 0.29888995084351816 2.6487551954229506E-4 44-45 4.414591992371585E-5 0.0 0.0 0.38592363197312396 3.3109439942786886E-4 46-47 4.414591992371585E-5 0.0 0.0 0.48335367724476486 3.752403193515847E-4 48-49 4.414591992371585E-5 0.0 0.0 0.5820418812342316 5.076780791227324E-4 50-51 4.414591992371585E-5 0.0 0.0 0.693686912721309 5.738969590083061E-4 52-53 4.414591992371585E-5 0.0 0.0 0.7940968075878008 5.738969590083061E-4 54-55 4.414591992371585E-5 0.0 0.0 0.9025191869204469 5.738969590083061E-4 56-57 4.414591992371585E-5 0.0 0.0 1.0315577108574683 5.738969590083061E-4 58-59 4.414591992371585E-5 0.0 0.0 1.1655847237458696 5.738969590083061E-4 60-61 4.414591992371585E-5 0.0 0.0 1.3135397743702033 5.738969590083061E-4 62-63 4.414591992371585E-5 4.414591992371585E-5 0.0 1.4507011475731884 6.180428789320219E-4 64-65 4.414591992371585E-5 4.414591992371585E-5 0.0 1.593226250046905 6.180428789320219E-4 66-67 4.414591992371585E-5 4.414591992371585E-5 0.0 1.7630997499133638 6.180428789320219E-4 68-69 4.414591992371585E-5 4.414591992371585E-5 0.0 1.949152729431864 6.180428789320219E-4 70-71 4.414591992371585E-5 4.414591992371585E-5 0.0 2.1425118586977394 6.401158388938798E-4 72-73 4.414591992371585E-5 4.414591992371585E-5 0.0 2.3383652324393047 7.063347187794536E-4 74-75 4.414591992371585E-5 4.414591992371585E-5 0.0 2.5307531514668584 7.504806387031694E-4 76-77 4.414591992371585E-5 4.414591992371585E-5 0.0 2.7398503011855384 7.504806387031694E-4 78-79 4.414591992371585E-5 4.414591992371585E-5 0.0 2.9727421017431017 7.946265586268853E-4 80-81 4.414591992371585E-5 4.414591992371585E-5 0.0 3.214617597005141 7.946265586268853E-4 82-83 4.414591992371585E-5 4.414591992371585E-5 0.0 3.46647007016994 8.387724785506012E-4 84-85 4.414591992371585E-5 4.414591992371585E-5 0.0 3.728939637076392 8.387724785506012E-4 86-87 4.414591992371585E-5 4.414591992371585E-5 0.0 3.988009968148719 8.60845438512459E-4 88-89 4.414591992371585E-5 4.414591992371585E-5 0.0 4.268049611184811 8.82918398474317E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5930 0.0 44.025326 1 GTATCAA 9695 0.0 37.217224 1 TATCAAC 11975 0.0 29.803568 2 ATCAACG 12015 0.0 29.704346 3 TCAACGC 12140 0.0 29.398493 4 CAACGCA 12295 0.0 28.989222 5 AACGCAG 12665 0.0 28.179842 6 ACGCAGA 14065 0.0 25.284695 7 CGCAGAG 14285 0.0 24.828724 8 TAAGGTG 1510 0.0 24.548304 5 GTAAGGT 1335 0.0 22.782558 4 GAGTACT 8850 0.0 22.721916 12-13 TACATGG 8290 0.0 22.242369 2 GTACATG 8455 0.0 22.128014 1 GCAGAGT 15820 0.0 21.907745 9 ACATGGG 8185 0.0 21.656782 3 AGGTAAG 1300 0.0 21.568129 2 TGGTATC 3110 0.0 21.545797 2 GTACTTT 9495 0.0 21.187511 14-15 AGAGTAC 13030 0.0 21.143927 10-11 >>END_MODULE