FastQCFastQC Report
Thu 26 May 2016
SRR938229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265215
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96210.4247278955860702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.3653074873687487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51720.22832269784545836No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27710.12232834410861662No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25630.11314599276448373No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25230.1113801559675351No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24780.10939358957096788No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23290.10281584750233422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.046.2855071
GTATCAA90550.037.1984061
ATCAACG111300.030.0873433
TATCAAC112600.029.8296132
TCAACGC112900.029.6169174
CAACGCA114550.029.2317715
TACGTCG650.005847392629.228585
AACGCAG118500.028.2974626
ACGCAGA134100.024.9347487
CGCAGAG136050.024.4028038
TAAGGTG13700.022.5348115
GAGTACT85300.022.4123612-13
GTAAGGT13050.022.2014034
GCAGAGT150350.021.9554589
GTACTTT89450.021.21324514-15
AGAGTAC123550.020.87483210-11
CAGAGTA141100.020.3486310-11
AGTACTT88700.019.81293512-13
GTACATG81600.019.736771
AGGTAAG13800.019.6228622
Produced by FastQC (version 0.11.5)