##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938229_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2265215 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5538772257821 34.0 31.0 34.0 31.0 34.0 2 32.76853146390078 34.0 31.0 34.0 31.0 34.0 3 32.82497820295204 34.0 31.0 34.0 31.0 34.0 4 36.269394295905684 37.0 37.0 37.0 35.0 37.0 5 36.23252715525899 37.0 37.0 37.0 35.0 37.0 6 36.27107625545478 37.0 37.0 37.0 35.0 37.0 7 36.23768295724688 37.0 37.0 37.0 35.0 37.0 8 36.22946519425309 37.0 37.0 37.0 35.0 37.0 9 37.947073898062655 39.0 38.0 39.0 35.0 39.0 10-11 37.935942062894696 39.0 38.0 39.0 35.0 39.0 12-13 37.88696989027532 39.0 38.0 39.0 35.0 39.0 14-15 39.230071538463235 41.0 39.0 41.0 36.0 41.0 16-17 39.18849049648709 40.0 39.0 41.0 36.0 41.0 18-19 39.217026639855376 40.0 39.0 41.0 36.0 41.0 20-21 39.1482062409087 40.0 39.0 41.0 36.0 41.0 22-23 39.01196310284013 40.0 38.5 41.0 35.5 41.0 24-25 38.97706266292603 40.0 38.5 41.0 35.0 41.0 26-27 38.94139607057167 40.0 38.0 41.0 35.0 41.0 28-29 38.890962668002814 40.0 38.0 41.0 35.0 41.0 30-31 38.69802667737941 40.0 38.0 41.0 35.0 41.0 32-33 38.526495498219816 40.0 38.0 41.0 34.5 41.0 34-35 38.33460355860261 40.0 38.0 41.0 34.0 41.0 36-37 38.21046302448112 40.0 38.0 41.0 34.0 41.0 38-39 38.19520266288189 40.0 38.0 41.0 34.0 41.0 40-41 38.13612968305437 40.0 38.0 41.0 34.0 41.0 42-43 37.98216085448843 40.0 38.0 41.0 33.0 41.0 44-45 37.780712206126125 40.0 37.5 41.0 33.0 41.0 46-47 37.467406625861116 40.0 37.0 41.0 33.0 41.0 48-49 37.130679427780585 40.0 37.0 41.0 31.5 41.0 50-51 36.8387726992802 40.0 36.0 41.0 31.0 41.0 52-53 36.440489092646835 39.0 36.0 41.0 30.0 41.0 54-55 36.22672505700342 39.0 35.0 41.0 30.0 41.0 56-57 35.98240453996641 39.0 35.0 41.0 29.0 41.0 58-59 35.65843838222862 38.5 35.0 40.0 28.5 41.0 60-61 35.30774562238022 38.0 35.0 40.0 28.0 41.0 62-63 34.950767587182675 37.5 34.0 40.0 28.0 41.0 64-65 34.572279673231904 37.0 34.0 40.0 27.0 41.0 66-67 34.16241570888414 36.5 34.0 39.0 26.0 41.0 68-69 33.719060000927065 36.0 33.5 39.0 26.0 40.5 70-71 33.21762459634074 35.5 33.0 38.5 25.0 40.0 72-73 32.81007608549299 35.0 33.0 37.5 24.5 40.0 74-75 32.3717018031401 35.0 32.0 37.0 23.5 39.0 76-77 31.073585730272843 34.0 30.5 35.5 22.0 38.5 78-79 31.78076827144443 35.0 32.0 36.0 23.0 39.0 80-81 31.626942917118242 35.0 32.0 36.0 22.5 37.5 82-83 31.269569555207784 35.0 32.0 35.5 20.5 37.0 84-85 30.90119569224113 34.5 31.0 35.0 19.5 37.0 86-87 30.49618601324819 34.0 31.0 35.0 18.0 36.0 88-89 30.05533183384359 34.0 31.0 35.0 13.0 36.0 90-91 29.725468884851992 34.0 30.0 35.0 7.0 35.5 92-93 29.344707676754744 34.0 30.0 35.0 2.0 35.0 94-95 28.958717825901736 34.0 29.5 35.0 2.0 35.0 96-97 28.420932891579827 34.0 29.0 35.0 2.0 35.0 98-99 27.78175934734672 34.0 29.0 35.0 2.0 35.0 100-101 25.394789457071404 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.061718695841207705 1101 2 0.08553738087607599 1101 3 0.037105792273223415 1101 4 -0.023613226094155948 1101 5 0.01985344262650557 1101 6 0.025330717115473078 1101 7 0.04675520195827687 1101 8 0.08311437611826022 1101 9 -0.0312955024514423 1101 10-11 -0.02473100256709415 1101 12-13 0.034110576907558254 1101 14-15 0.07720977934787498 1101 16-17 0.07492645169302392 1101 18-19 0.1749645980261434 1101 20-21 0.11648993183118961 1101 22-23 0.10502204046287744 1101 24-25 0.1223969097046762 1101 26-27 0.13630861901262392 1101 28-29 0.12719143316213177 1101 30-31 0.08358863213750567 1101 32-33 0.016300721203371893 1101 34-35 0.03668242726341475 1101 36-37 0.018035360695087377 1101 38-39 0.1398346077204522 1101 40-41 0.10218448429732518 1101 42-43 0.28334638776055954 1101 44-45 0.2565541062255079 1101 46-47 0.3620367861100817 1101 48-49 0.3882120191335332 1101 50-51 0.3638799388115288 1101 52-53 0.4409104044036738 1101 54-55 0.4820838649335286 1101 56-57 0.5013778983903663 1101 58-59 0.46350087137645346 1101 60-61 0.36782486246367085 1101 62-63 0.3115166807333978 1101 64-65 0.3140804382960809 1101 66-67 0.25549992642880426 1101 68-69 0.2034453085332828 1101 70-71 0.14397018292454788 1101 72-73 0.17630560632827041 1101 74-75 0.14750817337957045 1101 76-77 -0.028840266219575028 1101 78-79 0.1489974604526516 1101 80-81 0.3597224399011303 1101 82-83 0.2649202590288411 1101 84-85 0.3238948970402724 1101 86-87 0.3765531595487239 1101 88-89 0.3478551891434165 1101 90-91 0.37897380660128377 1101 92-93 0.4841654214635298 1101 94-95 0.2865820645755086 1101 96-97 0.14288876498198633 1101 98-99 0.22315838078543848 1101 100-101 0.49554865088823874 1102 1 0.10120418949161092 1102 2 0.1633495582153941 1102 3 0.13528718571385667 1102 4 0.0866066076700065 1102 5 0.14539725276201665 1102 6 0.06814683361766782 1102 7 0.10705682476682199 1102 8 0.12422194004923881 1102 9 0.13654131179409035 1102 10-11 0.12899720152267236 1102 12-13 0.15627379957520304 1102 14-15 0.2550446582884902 1102 16-17 0.17423303774132393 1102 18-19 0.21351632023658595 1102 20-21 0.13773939527185775 1102 22-23 0.18709808476290135 1102 24-25 0.08826053029631709 1102 26-27 -0.0880410763482331 1102 28-29 -0.02115087534550497 1102 30-31 0.10830476983840498 1102 32-33 0.06301961473628381 1102 34-35 0.08300595434871383 1102 36-37 0.12930234207291136 1102 38-39 0.17161434470882142 1102 40-41 0.2242751989468914 1102 42-43 0.17612930877374566 1102 44-45 0.27558047383219275 1102 46-47 0.20408769253941017 1102 48-49 0.24706540633901142 1102 50-51 0.10837618474185007 1102 52-53 0.04813302036640721 1102 54-55 0.09438006466965021 1102 56-57 0.20872696833661308 1102 58-59 0.08996355585003357 1102 60-61 0.04312314726672639 1102 62-63 0.20053248062603046 1102 64-65 0.14424952172091565 1102 66-67 0.10985478436055018 1102 68-69 0.1244552054822563 1102 70-71 0.2017317839437709 1102 72-73 0.12976429152324442 1102 74-75 0.09255157799751146 1102 76-77 0.11266137219796235 1102 78-79 0.1024491585113303 1102 80-81 0.17492230891210525 1102 82-83 0.29970340995836864 1102 84-85 0.21279178378327757 1102 86-87 0.30211919668759535 1102 88-89 0.17888540415737353 1102 90-91 0.13619916468542925 1102 92-93 0.24841469717434705 1102 94-95 0.11697473636150235 1102 96-97 0.178306558818754 1102 98-99 0.18903057050649252 1102 100-101 0.22736324445838463 1103 1 0.14649300576110136 1103 2 0.13458353998518646 1103 3 0.043200474036865444 1103 4 -0.006643384622748272 1103 5 0.13025096919695045 1103 6 0.07520256215811827 1103 7 0.07029787665201326 1103 8 0.08925467016197075 1103 9 0.0761750633740732 1103 10-11 0.027317792206154934 1103 12-13 0.05715268274359886 1103 14-15 0.1674284072993899 1103 16-17 0.16273099788971734 1103 18-19 0.07557842010196936 1103 20-21 0.08342051045924848 1103 22-23 0.250058335916421 1103 24-25 0.22475255072566114 1103 26-27 0.2285277082701569 1103 28-29 0.19328557068834584 1103 30-31 0.09038978592086266 1103 32-33 0.1560728152994315 1103 34-35 0.11096707899032765 1103 36-37 0.0292990022654962 1103 38-39 0.06243937990524984 1103 40-41 0.23054042076149273 1103 42-43 0.3267384382657923 1103 44-45 0.2920549731179847 1103 46-47 0.40357182074691167 1103 48-49 0.3225093438178135 1103 50-51 0.2984045474141155 1103 52-53 0.26311257046865677 1103 54-55 0.22000049812527323 1103 56-57 0.2723449046439157 1103 58-59 0.4261054103166373 1103 60-61 0.26337779473720957 1103 62-63 0.4279713107685623 1103 64-65 0.24419711243522357 1103 66-67 0.17351716579738508 1103 68-69 0.16172976848130105 1103 70-71 0.17936971799028356 1103 72-73 0.1626852870941491 1103 74-75 0.08821907704695064 1103 76-77 0.25926607259155077 1103 78-79 0.11977864849705 1103 80-81 0.13665351093448663 1103 82-83 0.12200946991048056 1103 84-85 0.3254260183508002 1103 86-87 0.31359914242856846 1103 88-89 0.052621672410705145 1103 90-91 0.08319410873080813 1103 92-93 0.13648439124798628 1103 94-95 0.053790204294212884 1103 96-97 0.14858304349160534 1103 98-99 0.09463476735988152 1103 100-101 0.25951248760664214 1104 1 0.18337885681732757 1104 2 0.12967749316339905 1104 3 0.14494855091413683 1104 4 0.07716427928922798 1104 5 0.05083731323944107 1104 6 0.05147968235626621 1104 7 0.04542945873303239 1104 8 0.11474229787398826 1104 9 0.14830824111110275 1104 10-11 0.10393905015466487 1104 12-13 0.1730056442335126 1104 14-15 0.21920863545438607 1104 16-17 0.11955064112961367 1104 18-19 0.15939799189458626 1104 20-21 0.1370345522320946 1104 22-23 0.15917500933088746 1104 24-25 0.13432053281621137 1104 26-27 0.13873157118663926 1104 28-29 0.04609463973023509 1104 30-31 0.12749593276885207 1104 32-33 0.11334138563928065 1104 34-35 0.16970746337671727 1104 36-37 0.16169677311023634 1104 38-39 0.17478189818385204 1104 40-41 0.20754868483064826 1104 42-43 0.21298007297964716 1104 44-45 0.19261107114365927 1104 46-47 0.1599687342242433 1104 48-49 0.1415810054434843 1104 50-51 0.08098689040870255 1104 52-53 0.09415522449709357 1104 54-55 0.0566136587149515 1104 56-57 0.09579628476728175 1104 58-59 0.17437995841257248 1104 60-61 0.16717164550764352 1104 62-63 0.19011853980473603 1104 64-65 0.26132421715312404 1104 66-67 0.3046550828709016 1104 68-69 0.4996456512124823 1104 70-71 0.3702280380264824 1104 72-73 0.18110201528486414 1104 74-75 0.2579301918627337 1104 76-77 0.23332594541538398 1104 78-79 0.41750067093149923 1104 80-81 0.39326833862745403 1104 82-83 0.34253746533785545 1104 84-85 0.39832130044849023 1104 86-87 0.2605126197678693 1104 88-89 0.21705402374081828 1104 90-91 0.20418174835070602 1104 92-93 0.2203527853512064 1104 94-95 0.08525558808520728 1104 96-97 0.31642938419988553 1104 98-99 0.5496587021999595 1104 100-101 0.6824434933462413 1105 1 0.11044915011925127 1105 2 0.10900108224192451 1105 3 0.06087977965586333 1105 4 0.03858274603052081 1105 5 0.09512077276022524 1105 6 0.10105911766291342 1105 7 0.07815534581601469 1105 8 0.04782022520993934 1105 9 0.051826045557724854 1105 10-11 0.050890199698152117 1105 12-13 0.14054692669060387 1105 14-15 0.17307025289098732 1105 16-17 0.13411062328943757 1105 18-19 0.13622254984157678 1105 20-21 0.16692896180689587 1105 22-23 0.12596057438147312 1105 24-25 0.18160499476403658 1105 26-27 0.18775566624183426 1105 28-29 0.06567022121370059 1105 30-31 0.05763510341743938 1105 32-33 0.09741589433095044 1105 34-35 0.09956918040649754 1105 36-37 0.17968730742766326 1105 38-39 0.074865145094833 1105 40-41 0.1926464052292829 1105 42-43 0.13386498006569525 1105 44-45 0.16775163826189043 1105 46-47 0.1492319395226005 1105 48-49 0.19021149091695122 1105 50-51 0.22992578999063085 1105 52-53 0.20338209993416712 1105 54-55 0.24912338528835676 1105 56-57 0.230133849367526 1105 58-59 0.32745305764419186 1105 60-61 0.2829986261670854 1105 62-63 0.38973467303443954 1105 64-65 0.327454307501462 1105 66-67 0.3338871977754607 1105 68-69 0.3520449809070598 1105 70-71 0.4040385165284306 1105 72-73 0.2663627653947387 1105 74-75 0.3744913474900713 1105 76-77 0.3111162324819077 1105 78-79 0.3856991601461992 1105 80-81 0.39540179782347806 1105 82-83 0.44421138859481246 1105 84-85 0.6277558493238438 1105 86-87 0.634750353027016 1105 88-89 0.44567786245182006 1105 90-91 0.4411886267755776 1105 92-93 0.5898649303069163 1105 94-95 0.3254667689356019 1105 96-97 0.6276602476944362 1105 98-99 0.8850641871862841 1105 100-101 0.8896403998953595 1106 1 -0.1664096110798141 1106 2 -0.07794826034023572 1106 3 -0.02609232268377326 1106 4 -0.08177305803452839 1106 5 -0.12080170023248371 1106 6 -0.08591698908647771 1106 7 0.026528493061427127 1106 8 -0.13679777453018716 1106 9 -0.13929360375404798 1106 10-11 -0.060808213493181995 1106 12-13 -0.06780394296094983 1106 14-15 -0.21630353505999267 1106 16-17 -0.19144012606638228 1106 18-19 -0.13514986353445835 1106 20-21 -0.057586851934111394 1106 22-23 -0.11947021021315862 1106 24-25 -0.08250983454595229 1106 26-27 -0.18633731451819813 1106 28-29 -0.029779890787189345 1106 30-31 -0.004991413457062777 1106 32-33 0.00510689446088719 1106 34-35 -0.06367221767187203 1106 36-37 -0.09587471965358674 1106 38-39 -0.1704262002315673 1106 40-41 -0.016083351049267947 1106 42-43 -0.09641085021597462 1106 44-45 -0.0186872442299304 1106 46-47 -0.03338262950075688 1106 48-49 -0.006428727576718529 1106 50-51 0.04186828830101064 1106 52-53 -0.0814759805152434 1106 54-55 -0.1565445706892845 1106 56-57 0.023771087436230687 1106 58-59 -0.049515538793855285 1106 60-61 -0.18838414555458627 1106 62-63 -0.052230907837888196 1106 64-65 -0.26825445668048786 1106 66-67 -0.23273862118762878 1106 68-69 -0.21710290112793018 1106 70-71 -0.3039788225658029 1106 72-73 -0.10984979439559339 1106 74-75 -0.3754725923914819 1106 76-77 -0.23505593997575858 1106 78-79 -0.20480918332754783 1106 80-81 -0.37739504130794543 1106 82-83 -0.3493819235505633 1106 84-85 -0.25936443660735264 1106 86-87 -0.23879386015141435 1106 88-89 -0.15711574454886446 1106 90-91 -0.30678485585958626 1106 92-93 -0.12632967910377246 1106 94-95 -0.17049265903855115 1106 96-97 -0.1717795026919049 1106 98-99 -0.05598865386071239 1106 100-101 -0.00300403058359322 1107 1 0.06872720108629693 1107 2 0.029594691428002307 1107 3 0.053469031690575264 1107 4 0.09373677332203556 1107 5 0.028437115648991096 1107 6 0.05173537573475784 1107 7 0.022655134964075785 1107 8 0.04070843621564535 1107 9 0.040245093147035504 1107 10-11 0.04177731751317282 1107 12-13 0.08430048926874889 1107 14-15 0.1087247008130987 1107 16-17 0.12085856438515208 1107 18-19 0.07192119763765703 1107 20-21 0.07026051999003613 1107 22-23 0.046434170664703345 1107 24-25 -0.04257393647325358 1107 26-27 -0.0957404715868364 1107 28-29 -0.07466642766308951 1107 30-31 -0.03982278912867088 1107 32-33 -0.14877135428836397 1107 34-35 -0.12754489566294325 1107 36-37 -0.057421013876727045 1107 38-39 -0.007706951924767225 1107 40-41 0.07915244714715897 1107 42-43 0.0431253707925805 1107 44-45 0.05349008545884715 1107 46-47 0.14119683174808983 1107 48-49 0.08914989385549177 1107 50-51 0.08371641216288594 1107 52-53 0.15514588956305886 1107 54-55 0.10688848354575953 1107 56-57 0.06628384324979919 1107 58-59 -3.625767781727518E-4 1107 60-61 0.07627386414876725 1107 62-63 0.04678043541786536 1107 64-65 0.11629950822450752 1107 66-67 0.04581017720212799 1107 68-69 0.2962320535074525 1107 70-71 0.1745287682608634 1107 72-73 0.14655476262214506 1107 74-75 0.16650381556225113 1107 76-77 0.13124557711974205 1107 78-79 0.31203767966313123 1107 80-81 0.3541300435334058 1107 82-83 0.3810573792564398 1107 84-85 0.5409285631121996 1107 86-87 0.4740650774003079 1107 88-89 0.32864596302224314 1107 90-91 0.425971966408774 1107 92-93 0.5548962927031447 1107 94-95 0.5476374842651914 1107 96-97 0.5411206997352345 1107 98-99 0.5500145067230768 1107 100-101 0.7027149372748873 1108 1 0.05744855009263716 1108 2 0.05995999637274707 1108 3 0.1180885430017895 1108 4 0.06407862405779241 1108 5 -0.031139886272896433 1108 6 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-0.06286115097849176 2316 92-93 -0.45562629489673867 2316 94-95 -0.22419761136003657 2316 96-97 -0.3733492790367592 2316 98-99 -0.10669537372377036 2316 100-101 -0.030407750611654905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 13.0 5 29.0 6 55.0 7 166.0 8 438.0 9 842.0 10 1419.0 11 2359.0 12 3673.0 13 5165.0 14 7225.0 15 10154.0 16 13168.0 17 18579.0 18 18632.0 19 13185.0 20 10893.0 21 10960.0 22 10809.0 23 11830.0 24 13349.0 25 15873.0 26 18647.0 27 22722.0 28 27793.0 29 34473.0 30 42092.0 31 51997.0 32 65737.0 33 85945.0 34 121710.0 35 187530.0 36 319078.0 37 541661.0 38 508279.0 39 68720.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18044865498418 18.774332679237954 15.143419057352173 22.90179960842569 2 15.780329338210782 19.74682594173111 40.092677423841366 24.38016729621674 3 19.623074501680527 23.528970275881953 29.75930470808097 27.088650514356555 4 13.130056087391262 16.715013806636456 36.38828985328104 33.766640252691246 5 13.846853389192637 37.13029447535885 34.149959275388866 14.872892860059642 6 32.748826380200725 35.91091172842831 16.791040493304386 14.549221398066583 7 28.871299192350396 32.80430334427417 19.90230507920882 18.422092384166625 8 26.707292792850463 34.84093520730317 19.08839316813592 19.363378831710452 9 26.123803091104136 17.019216761359875 20.01311136715801 36.84386878037798 10-11 25.320355904406426 26.522603814648942 26.58619601229905 21.57084426864558 12-13 26.212478727184834 23.723620053725586 27.237856009252987 22.826045209836593 14-15 22.78794728094243 25.220321249859285 25.73872678752348 26.253004681674806 16-17 22.171434625746205 28.4362028644175 26.72183453392239 22.670527975913906 18-19 22.130658679198223 26.74534205362405 29.236253512359756 21.887745754817974 20-21 22.630876097853847 25.84719331277605 29.634096542712278 21.887834046657822 22-23 22.469677271252397 25.691424434325217 29.610500548513052 22.22839774590933 24-25 22.33468978708422 25.923299107433568 29.562193238922386 22.17981786655983 26-27 21.820109810460053 26.223633335769247 29.535278194373216 22.420978659397484 28-29 22.024399449941836 26.641334266283774 29.338473389943122 21.995792893831272 30-31 22.07565286297327 26.147407641217278 29.217535642312097 22.55940385349735 32-33 21.790257393922406 26.126134279237416 29.342112524032043 22.741495802808135 34-35 22.529941309558083 26.32612051410213 29.035276964840484 22.1086612114993 36-37 22.59820160908661 25.901358682215992 29.106353878972392 22.394085829725014 38-39 22.07200971921698 26.296526633908684 29.05658202973651 22.574881617137823 40-41 22.625780956796255 26.113318762831977 29.057111950018765 22.203788330353003 42-43 22.240637557143522 26.126518501708922 28.850334908668597 22.782509032478956 44-45 22.266769205910958 26.40841728292488 28.988556602678234 22.336256908485932 46-47 22.771635363530613 26.666166346240868 28.22451731954803 22.337680970680488 48-49 22.466057305818655 26.756201949925284 28.18478599161669 22.592954752639375 50-51 22.526316486514524 26.974702180587716 28.17677350715054 22.322207825747224 52-53 22.887562549250294 26.64438033475851 28.053871266082908 22.414185849908286 54-55 22.497467127844377 26.917202119887072 28.092189924576694 22.493140827691853 56-57 22.475460386762407 27.13861598126447 27.906379747617777 22.479543884355348 58-59 22.509651401743323 26.696936052427695 27.91984425319451 22.873568292634474 60-61 22.13112221135742 26.922389265478113 28.25742810285116 22.689060420313304 62-63 22.26534788088548 26.89111188121216 28.41739084369475 22.426149394207613 64-65 22.070840957701588 26.992029454157773 28.15876197182166 22.77836761631898 66-67 21.96444929068543 27.037102438399884 28.051487386407032 22.946960884507654 68-69 22.458695321558558 26.69499222919558 28.20761170214057 22.63870074710529 70-71 22.38699196323528 26.7796875793247 27.798133951964825 23.035186505475195 72-73 22.282277841176224 26.91914454036372 27.767320099858072 23.031257518601986 74-75 22.201049348516584 27.339700646516995 27.60203777566368 22.857212229302736 76-77 22.28033542069958 26.901883485673544 27.873711766874226 22.944069326752647 78-79 22.379487156848246 27.116233999863148 27.767430464657885 22.736848378630725 80-81 22.571345324836713 27.117403866741125 27.4651633509402 22.84608745748196 82-83 22.44928185624764 27.142015217098596 27.635147215606466 22.773555711047297 84-85 22.3394822365794 27.31324441929032 27.561683096435484 22.785590247694795 86-87 22.53221119845171 27.22569161480236 27.359829525438307 22.882267661307626 88-89 22.385280154546106 27.387279965601486 27.139930267074103 23.087509612778305 90-91 22.253256872431255 27.369427117132627 27.303495040194946 23.07382097024117 92-93 22.36390392857529 27.017533249117463 27.468175694929897 23.150387127377346 94-95 22.20928256258236 26.84575194849054 27.592149089600763 23.352816399326333 96-97 22.39590943905987 26.557103851069325 27.654814222932483 23.392172486938325 98-99 22.13968651982262 26.862659835821322 27.301448206903096 23.696205437452956 100-101 22.155821853554738 26.554786190273326 27.69505322894295 23.594338727228983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 670.0 1 468.0 2 368.5 3 503.5 4 431.0 5 375.0 6 794.5 7 1845.0 8 3085.0 9 3634.5 10 3055.5 11 2337.0 12 2248.0 13 2359.5 14 2496.5 15 2609.0 16 2696.0 17 2899.0 18 3140.5 19 3426.5 20 3863.5 21 4569.0 22 5427.0 23 6602.5 24 8330.0 25 10544.5 26 13517.5 27 17066.0 28 20161.5 29 23008.5 30 26375.0 31 31178.5 32 37859.0 33 41005.5 34 41155.0 35 43695.0 36 47978.5 37 52479.0 38 56085.5 39 58992.5 40 62560.5 41 67368.0 42 69623.5 43 71417.0 44 75008.5 45 78689.0 46 80206.5 47 82718.5 48 84916.0 49 83125.0 50 80946.0 51 79336.5 52 78835.5 53 79990.0 54 82387.0 55 78317.0 56 71504.5 57 66001.0 58 61108.0 59 54313.0 60 43215.5 61 35764.5 62 30274.5 63 21747.5 64 14662.5 65 10984.0 66 8143.0 67 6368.5 68 4931.0 69 3697.5 70 2764.0 71 1701.0 72 1146.5 73 861.0 74 578.5 75 403.0 76 280.5 77 146.0 78 74.5 79 36.5 80 21.0 81 15.5 82 11.0 83 5.5 84 2.0 85 1.0 86 2.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016333990371774864 3 0.00671017982840481 4 0.0 5 0.0 6 4.4145919923715855E-5 7 0.0 8 2.6487551954229506E-4 9 2.2072959961857923E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.3109439942786886E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005187145591036613 26-27 0.02183015740227749 28-29 0.0 30-31 0.0 32-33 4.4145919923715846E-4 34-35 0.019909809885595847 36-37 0.0034875276739735517 38-39 0.01869579708769366 40-41 0.016113260772156284 42-43 0.008586381425162734 44-45 0.002913630714965246 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.428025595804372E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0030239955147745357 86-87 3.9731327931344265E-4 88-89 4.4145919923715855E-5 90-91 2.2072959961857923E-4 92-93 1.9865663965672133E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2265215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.93073533933283 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78187695608865 40.11190472680614 2 13.53719911873874 14.330678075796197 3 4.467603778387027 7.094206595844213 4 2.0480307992182745 4.3361510480089915 5 1.0862869535103137 2.8748983619412276 6 0.6607942126659065 2.0985794150629142 7 0.44654249942743685 1.6545075998470486 8 0.3234895672730682 1.369803253629286 9 0.2323840021309957 1.1070230502501621 >10 1.2635317635521413 12.308425244526815 >50 0.09700480469091659 3.52203600407026 >100 0.04890691788246873 4.756268940733747 >500 0.0033413823337560475 1.1839130496924162 >1k 0.002756640425348662 2.2316424949235993 >5k 2.5060367503169657E-4 1.0199621388670468 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9621 0.4247278955860702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8275 0.3653074873687487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5172 0.22832269784545836 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2771 0.12232834410861662 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2563 0.11314599276448373 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2523 0.1113801559675351 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2478 0.10939358957096788 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2329 0.10281584750233422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.414591992371585E-5 2 0.0 0.0 0.0 0.0 4.414591992371585E-5 3 0.0 0.0 0.0 0.0 4.414591992371585E-5 4 0.0 0.0 0.0 0.0 4.414591992371585E-5 5 0.0 0.0 0.0 0.0 4.414591992371585E-5 6 0.0 0.0 0.0 0.0 4.414591992371585E-5 7 0.0 0.0 0.0 0.0 4.414591992371585E-5 8 0.0 0.0 0.0 0.0 4.414591992371585E-5 9 0.0 0.0 0.0 4.414591992371585E-5 4.414591992371585E-5 10-11 0.0 0.0 0.0 1.1036479980928963E-4 4.414591992371585E-5 12-13 0.0 0.0 0.0 1.3243775977114756E-4 8.82918398474317E-5 14-15 0.0 0.0 0.0 1.5451071973300546E-4 1.1036479980928963E-4 16-17 0.0 0.0 0.0 1.765836796948634E-4 1.3243775977114756E-4 18-19 0.0 0.0 0.0 1.765836796948634E-4 2.2072959961857926E-4 20-21 0.0 0.0 0.0 2.648755195422951E-4 3.9731327931344265E-4 22-23 0.0 0.0 0.0 3.090214394660109E-4 4.856051191608744E-4 24-25 0.0 0.0 0.0 6.621887988557377E-4 4.856051191608744E-4 26-27 0.0 0.0 0.0 0.0011036479980928962 4.856051191608744E-4 28-29 0.0 0.0 0.0 0.0030681414346982517 4.856051191608744E-4 30-31 0.0 0.0 0.0 0.010926115181119673 4.856051191608744E-4 32-33 0.0 0.0 0.0 0.029025942349843174 4.856051191608744E-4 34-35 0.0 2.2072959961857924E-5 0.0 0.056550923422280006 4.856051191608744E-4 36-37 0.0 4.414591992371585E-5 0.0 0.1014031780647753 4.856051191608744E-4 38-39 0.0 4.414591992371585E-5 0.0 0.16561341859382 5.297510390845902E-4 40-41 0.0 4.414591992371585E-5 0.0 0.23333325975680014 5.297510390845902E-4 42-43 0.0 4.414591992371585E-5 0.0 0.30120761163951326 5.297510390845902E-4 44-45 0.0 4.414591992371585E-5 0.0 0.3889917734078222 6.180428789320219E-4 46-47 0.0 4.414591992371585E-5 0.0 0.4868191319587766 7.284076787413116E-4 48-49 0.0 4.414591992371585E-5 0.0 0.5860591599472897 8.387724785506012E-4 50-51 0.0 4.414591992371585E-5 0.0 0.6980573587937569 9.04991358436175E-4 52-53 0.0 4.414591992371585E-5 0.0 0.799328099098761 9.270643183980329E-4 54-55 0.0 4.414591992371585E-5 0.0 0.9081257187507588 9.712102383217487E-4 56-57 0.0 4.414591992371585E-5 0.0 1.037340826367475 0.0010153561582454645 58-59 0.0 4.414591992371585E-5 0.0 1.1716106418154568 0.0010374291182073225 60-61 0.0 4.414591992371585E-5 0.0 1.3197864220394089 0.0010595020781691805 62-63 0.0 4.414591992371585E-5 0.0 1.45760998404125 0.0010595020781691805 64-65 0.0 4.414591992371585E-5 0.0 1.6010621508333647 0.0010815750381310382 66-67 0.0 4.414591992371585E-5 0.0 1.771112234379518 0.0011036479980928962 68-69 0.0 4.414591992371585E-5 0.0 1.9578715486167981 0.0011036479980928962 70-71 0.0 4.414591992371585E-5 0.0 2.152157742201071 0.0011257209580547542 72-73 0.0 4.414591992371585E-5 0.0 2.348916107301073 0.0012360857578640437 74-75 0.0 4.414591992371585E-5 0.0 2.54324644680527 0.0013464505576733334 76-77 0.0 4.414591992371585E-5 0.0 2.7543743088404415 0.0013685235176351914 78-79 0.0 4.414591992371585E-5 0.0 2.989782426833656 0.0013685235176351914 80-81 0.0 4.414591992371585E-5 0.0 3.2353220334493633 0.0013685235176351914 82-83 0.0 4.414591992371585E-5 0.0 3.4899556995693564 0.0013685235176351914 84-85 0.0 4.414591992371585E-5 0.0 3.754919510951499 0.0013685235176351914 86-87 0.0 4.414591992371585E-5 0.0 4.017234567138218 0.0014347423975207651 88-89 0.0 4.414591992371585E-5 0.0 4.3022847720856525 0.0015009612774063389 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5635 0.0 46.285507 1 GTATCAA 9055 0.0 37.198406 1 ATCAACG 11130 0.0 30.087343 3 TATCAAC 11260 0.0 29.829613 2 TCAACGC 11290 0.0 29.616917 4 CAACGCA 11455 0.0 29.231771 5 TACGTCG 65 0.0058473926 29.22858 5 AACGCAG 11850 0.0 28.297462 6 ACGCAGA 13410 0.0 24.934748 7 CGCAGAG 13605 0.0 24.402803 8 TAAGGTG 1370 0.0 22.534811 5 GAGTACT 8530 0.0 22.41236 12-13 GTAAGGT 1305 0.0 22.201403 4 GCAGAGT 15035 0.0 21.955458 9 GTACTTT 8945 0.0 21.213245 14-15 AGAGTAC 12355 0.0 20.874832 10-11 CAGAGTA 14110 0.0 20.34863 10-11 AGTACTT 8870 0.0 19.812935 12-13 GTACATG 8160 0.0 19.73677 1 AGGTAAG 1380 0.0 19.622862 2 >>END_MODULE