FastQCFastQC Report
Thu 26 May 2016
SRR938228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220625
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94730.4265916127216437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78060.35152265690965384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.20863495637489446No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27000.12158739093723614No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24180.10888826343934703No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24060.10834787503518153No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23590.10623135378553335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.044.980521
GTATCAA98250.037.8358231
TATCAAC118900.030.7116492
ATCAACG119400.030.5040863
TCAACGC122250.029.792954
CAACGCA122350.029.7685955
AACGCAG125750.028.9982596
ACGCAGA140900.025.6435057
CGCAGAG142700.025.2499898
TAAGGTG12850.023.3115435
TACATGG80650.022.9334282
GAGTACT91950.022.64348412-13
GCAGAGT156050.022.6019699
GTACATG83650.022.4758361
GTAAGGT12900.021.7468244
ACATGGG82850.021.6936633
GTACTTT95900.021.68310714-15
TGGTATC31150.021.0641922
AGAGTAC131000.020.70072610-11
GTGTAAG9550.020.4345551
Produced by FastQC (version 0.11.5)