##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938228_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220625 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09181424148607 33.0 31.0 34.0 30.0 34.0 2 32.204877455671266 34.0 31.0 34.0 30.0 34.0 3 32.2322125527723 34.0 31.0 34.0 30.0 34.0 4 35.73499757951027 37.0 35.0 37.0 35.0 37.0 5 35.74834066985646 37.0 35.0 37.0 35.0 37.0 6 35.77569513087532 37.0 36.0 37.0 35.0 37.0 7 35.71635462989023 37.0 36.0 37.0 35.0 37.0 8 35.71024058542077 37.0 36.0 37.0 35.0 37.0 9 37.366223022797634 39.0 37.0 39.0 35.0 39.0 10-11 37.281183225443286 39.0 37.0 39.0 34.0 39.0 12-13 37.14493892485224 39.0 37.0 39.0 34.0 39.0 14-15 38.36438637770898 40.0 38.0 41.0 34.0 41.0 16-17 38.199008612440196 40.0 38.0 41.0 33.0 41.0 18-19 38.374596566282015 40.0 38.0 41.0 34.0 41.0 20-21 38.37974264002251 40.0 38.0 41.0 34.0 41.0 22-23 38.27589147199549 40.0 38.0 41.0 34.0 41.0 24-25 38.162086574725585 40.0 38.0 41.0 33.5 41.0 26-27 37.99141075147762 40.0 38.0 41.0 33.0 41.0 28-29 37.825929411764704 40.0 38.0 41.0 33.0 41.0 30-31 37.60098485786659 40.0 37.5 41.0 32.5 41.0 32-33 37.312109428651844 40.0 37.0 41.0 31.5 41.0 34-35 37.083880664227415 40.0 36.5 41.0 31.0 41.0 36-37 36.82993976920912 39.5 36.0 41.0 30.5 41.0 38-39 36.54089817056009 39.0 35.5 41.0 30.0 41.0 40-41 36.4320135097101 39.0 35.5 41.0 30.0 41.0 42-43 36.22158086124402 39.0 35.0 41.0 29.5 41.0 44-45 35.89772338868562 39.0 35.0 41.0 28.5 41.0 46-47 35.52400607936955 39.0 35.0 40.5 26.5 41.0 48-49 35.121603377427526 39.0 35.0 40.0 25.5 41.0 50-51 34.38755868280327 37.5 33.5 39.5 24.0 40.5 52-53 34.43849862088376 38.0 34.0 40.0 24.0 40.5 54-55 34.52442533070645 38.0 34.0 40.0 23.5 41.0 56-57 34.21503360540389 38.0 34.0 40.0 23.0 41.0 58-59 33.81735524908528 38.0 33.5 40.0 21.5 41.0 60-61 33.32954978891078 37.0 33.0 40.0 19.0 41.0 62-63 32.8023403321137 36.5 32.0 39.5 16.5 41.0 64-65 32.27639493385871 36.0 31.5 39.0 10.5 40.5 66-67 31.729108471714042 35.0 31.0 39.0 7.5 40.0 68-69 31.066710723332395 35.0 30.0 38.0 4.0 40.0 70-71 30.495849817056012 35.0 29.5 37.5 2.0 40.0 72-73 29.826777821559247 34.0 29.0 37.0 2.0 39.0 74-75 29.16897224880383 34.0 28.0 36.0 2.0 39.0 76-77 28.449573205741626 34.0 26.5 36.0 2.0 38.5 78-79 27.712531607092597 33.5 26.0 35.0 2.0 37.5 80-81 27.016586096256685 33.0 25.0 35.0 2.0 37.0 82-83 26.30398401350971 32.0 23.5 35.0 2.0 36.0 84-85 25.59830543202927 32.0 20.5 35.0 2.0 36.0 86-87 24.88039673515339 31.5 18.0 34.5 2.0 35.0 88-89 24.025973318322542 31.0 9.0 34.0 2.0 35.0 90-91 23.152881058260625 30.5 2.0 34.0 2.0 35.0 92-93 22.242779172530255 29.5 2.0 34.0 2.0 35.0 94-95 21.176513594145792 29.0 2.0 34.0 2.0 35.0 96-97 19.93200720517872 28.0 2.0 34.0 2.0 35.0 98-99 18.377824936673235 25.5 2.0 33.0 2.0 35.0 100-101 14.291615423585704 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21121643549793134 1101 2 0.17567588702554104 1101 3 0.20868846105691574 1101 4 0.12203721733024508 1101 5 0.24271998729270905 1101 6 0.14119464037222684 1101 7 0.1696352075176648 1101 8 0.30467123198096147 1101 9 0.22397736099004817 1101 10-11 0.300229011001953 1101 12-13 0.35221944792098014 1101 14-15 0.38564382515289 1101 16-17 0.34731047782729974 1101 18-19 0.37642464552777 1101 20-21 0.37778117287419377 1101 22-23 0.3821017844017973 1101 24-25 0.37583151852930285 1101 26-27 0.4453783895758008 1101 28-29 0.3883556486044668 1101 30-31 0.33213782349049126 1101 32-33 0.2393314689338908 1101 34-35 0.2931785398005786 1101 36-37 0.34535798343818414 1101 38-39 0.3854615295476549 1101 40-41 0.4162927599638877 1101 42-43 0.2793374773033932 1101 44-45 0.3107677800319735 1101 46-47 0.4286952052455959 1101 48-49 0.5859180332953002 1101 50-51 0.4966995614308658 1101 52-53 0.49039619719518157 1101 54-55 0.46119735764862213 1101 56-57 0.48899496958409117 1101 58-59 0.4664736440088717 1101 60-61 0.44030344550706957 1101 62-63 0.3012329304237511 1101 64-65 0.202554391734715 1101 66-67 0.1163280701042737 1101 68-69 0.07789012969633546 1101 70-71 -0.12594419438872606 1101 72-73 -0.1428183500985334 1101 74-75 -0.17040513859803852 1101 76-77 -0.2917923169159202 1101 78-79 -0.3715669821943983 1101 80-81 -0.4289943124575508 1101 82-83 -0.22679517927150172 1101 84-85 -0.47823051024951724 1101 86-87 -0.49399298780793544 1101 88-89 -0.3233055835994385 1101 90-91 -0.33799784066773597 1101 92-93 -0.5791898751510445 1101 94-95 -1.049374376896484 1101 96-97 -1.1536855755214575 1101 98-99 -1.1173904651697555 1101 100-101 -1.185543087913958 1102 1 0.12977744420538073 1102 2 0.1956521394285744 1102 3 0.15940823941267723 1102 4 0.05334955477228931 1102 5 0.10898369870286473 1102 6 0.16871527816483223 1102 7 0.058493061187235185 1102 8 0.157053342124577 1102 9 0.1714459802369106 1102 10-11 0.13490219883414056 1102 12-13 0.13881732194563767 1102 14-15 0.1683162779404057 1102 16-17 0.1444698128945774 1102 18-19 0.15126943801900694 1102 20-21 0.20943869860076347 1102 22-23 0.28352098603020437 1102 24-25 0.1361184686593475 1102 26-27 0.07691010958031796 1102 28-29 -0.009496041998268367 1102 30-31 -0.013237614709211698 1102 32-33 -0.050511910009902294 1102 34-35 -0.01435764701385267 1102 36-37 0.052358435773363965 1102 38-39 -0.20585533124619815 1102 40-41 -0.11322578577850351 1102 42-43 -0.01029867558589359 1102 44-45 0.06999372145456562 1102 46-47 -0.03525622526441197 1102 48-49 -0.1802894189267903 1102 50-51 -0.3060198211316134 1102 52-53 -0.21014999753511177 1102 54-55 -0.4110470729744762 1102 56-57 -0.4116165310378648 1102 58-59 -0.2804388856756219 1102 60-61 -0.32663989951158356 1102 62-63 -0.4191690824678034 1102 64-65 -0.6024492834886246 1102 66-67 -0.6344787827737086 1102 68-69 -0.6817624203432437 1102 70-71 -0.9965090479452137 1102 72-73 -1.0619922729753881 1102 74-75 -1.0794792650257214 1102 76-77 -1.0516797985398014 1102 78-79 -1.0409458694498532 1102 80-81 -1.2141416041006572 1102 82-83 -1.1941976444982352 1102 84-85 -1.232877688808827 1102 86-87 -1.1591580901487717 1102 88-89 -0.924299024739323 1102 90-91 -1.106209985867327 1102 92-93 -1.2855675184847541 1102 94-95 -1.2312363015826797 1102 96-97 -1.623830322779174 1102 98-99 -1.7064959723505773 1102 100-101 -1.7671754524961134 1103 1 0.20416443483196645 1103 2 0.19580464000850384 1103 3 0.218343269869834 1103 4 0.1366889738095125 1103 5 0.1795205866600469 1103 6 0.1015237374364375 1103 7 0.15458775760368582 1103 8 0.11701764410150162 1103 9 0.07574327474775089 1103 10-11 0.14585002215684284 1103 12-13 0.0409205412113991 1103 14-15 0.11228681311004607 1103 16-17 0.0835952328081504 1103 18-19 -0.007274782853379236 1103 20-21 0.10384976713581295 1103 22-23 0.06689283828936254 1103 24-25 0.13008341930071055 1103 26-27 -0.018911804108647345 1103 28-29 -0.008038157933079049 1103 30-31 -0.024075895361825417 1103 32-33 0.01475296846216878 1103 34-35 -0.18264364789018828 1103 36-37 -0.05430820225469546 1103 38-39 -0.10933535152233276 1103 40-41 -0.05752709685391011 1103 42-43 -0.06906670716854535 1103 44-45 -0.028010454054268052 1103 46-47 0.0834746047683197 1103 48-49 0.24168666640480296 1103 50-51 0.13353678880197606 1103 52-53 0.11650946652200389 1103 54-55 0.07961763624343376 1103 56-57 0.1929263753224859 1103 58-59 0.2322095339028749 1103 60-61 0.26883083326443824 1103 62-63 0.0611186066543965 1103 64-65 -0.18297227456196907 1103 66-67 -0.149819052377552 1103 68-69 -0.1707077392198073 1103 70-71 -0.4766012785776361 1103 72-73 -0.5735672817817594 1103 74-75 -0.5144608575613567 1103 76-77 -0.5317462544263272 1103 78-79 -0.5986281398613009 1103 80-81 -0.7005942902587599 1103 82-83 -0.8405595391138903 1103 84-85 -0.7444569934061853 1103 86-87 -0.7607428074427673 1103 88-89 -1.0138297525322315 1103 90-91 -0.8485505905428674 1103 92-93 -0.9726970065123872 1103 94-95 -1.077033514473488 1103 96-97 -1.3190482482557933 1103 98-99 -1.4567644592870757 1103 100-101 -1.518878733621266 1104 1 0.2415981410921404 1104 2 0.16639665175705431 1104 3 0.22341429583160277 1104 4 0.029800192811826776 1104 5 0.10728461053832916 1104 6 0.12678357077795965 1104 7 0.15569470130554208 1104 8 0.20739004263333527 1104 9 0.2507775563979635 1104 10-11 0.2298454569545001 1104 12-13 0.16323794565663263 1104 14-15 0.2538462043762735 1104 16-17 0.30308544184847364 1104 18-19 0.32870444853546843 1104 20-21 0.19431796369530474 1104 22-23 0.31724418131751975 1104 24-25 0.26736613081623517 1104 26-27 0.27886219580508964 1104 28-29 0.20607544949964307 1104 30-31 0.22794526747576072 1104 32-33 0.2253844931354294 1104 34-35 0.16653915982460887 1104 36-37 0.27557544075872187 1104 38-39 0.10567542199407853 1104 40-41 0.1520423737691985 1104 42-43 0.22929562304073414 1104 44-45 0.14337159048629644 1104 46-47 -0.09695446005233777 1104 48-49 -0.10894828392568456 1104 50-51 -0.18934369237496895 1104 52-53 -0.10053964827412898 1104 54-55 -0.04597345263406538 1104 56-57 0.02624909229498229 1104 58-59 -0.11048446211874818 1104 60-61 -0.018120748105204143 1104 62-63 0.002912778322453846 1104 64-65 -0.19635277630288783 1104 66-67 -0.06458902676788014 1104 68-69 0.03599744561019591 1104 70-71 -0.19634966581032032 1104 72-73 -0.20265198930686878 1104 74-75 -0.19191968196407316 1104 76-77 -0.5109545715007435 1104 78-79 -0.576299550620174 1104 80-81 -0.5581386106394675 1104 82-83 -0.766827659235588 1104 84-85 -0.6179347577309997 1104 86-87 -0.686369306481275 1104 88-89 -0.5830176209910043 1104 90-91 -0.84316558779933 1104 92-93 -0.8708048951539489 1104 94-95 -1.0379283583327314 1104 96-97 -1.4884209166270956 1104 98-99 -1.3555977295909898 1104 100-101 -0.8540428040443526 1105 1 0.17498435114084288 1105 2 0.13398618596084333 1105 3 0.18478029152374376 1105 4 0.17041917883720004 1105 5 0.1859140904868113 1105 6 0.10250862481121459 1105 7 0.1825262804087373 1105 8 0.16712925818898583 1105 9 0.22708342159611306 1105 10-11 0.16243365883160266 1105 12-13 0.3091589442354845 1105 14-15 0.22054324161480565 1105 16-17 0.29279240862172884 1105 18-19 0.19103126919689117 1105 20-21 0.17354002832054505 1105 22-23 0.19063474918475976 1105 24-25 0.16458996447524754 1105 26-27 0.24186410824901827 1105 28-29 0.23889926039807818 1105 30-31 0.2132644614921375 1105 32-33 0.05987840791833321 1105 34-35 0.16651059344513897 1105 36-37 0.2578597750024727 1105 38-39 0.06713740997352602 1105 40-41 0.26993142704005635 1105 42-43 0.3189307922092155 1105 44-45 0.28384543154712816 1105 46-47 0.3107235119613989 1105 48-49 0.3147637540160204 1105 50-51 0.2396090618403619 1105 52-53 0.22972436058584833 1105 54-55 0.324577015715775 1105 56-57 0.42839709898623113 1105 58-59 0.5336370858598158 1105 60-61 0.314450660904285 1105 62-63 0.31704834811417015 1105 64-65 0.2018994470172757 1105 66-67 0.30327678017798476 1105 68-69 0.23562636024506745 1105 70-71 -0.005451770146304824 1105 72-73 0.04738767979499414 1105 74-75 -0.15572122172662262 1105 76-77 -0.21902895696506164 1105 78-79 -0.22114647646138863 1105 80-81 -0.1426186470193862 1105 82-83 -0.40519556829689307 1105 84-85 -0.6589258931323982 1105 86-87 -0.4964556208955706 1105 88-89 -0.5336631286444806 1105 90-91 -0.5895577812901784 1105 92-93 -0.6712952191313875 1105 94-95 -0.7225372556843652 1105 96-97 -0.8638990276099072 1105 98-99 -0.9903580634498503 1105 100-101 -0.9927067857026532 1106 1 0.30955312694516124 1106 2 0.254061213225782 1106 3 0.24530626137651268 1106 4 0.27639141728855776 1106 5 0.33162570535009905 1106 6 0.31474011386628575 1106 7 0.36419645256734157 1106 8 0.2355501314459758 1106 9 0.3962652787278387 1106 10-11 0.40748531615029293 1106 12-13 0.4391929767197027 1106 14-15 0.480180058043878 1106 16-17 0.1967011538503627 1106 18-19 0.5028638868256294 1106 20-21 0.537328174889069 1106 22-23 0.5009199599037757 1106 24-25 0.39231731023360084 1106 26-27 0.31479477253974153 1106 28-29 0.16529999655138994 1106 30-31 0.22083510689214592 1106 32-33 0.20066614551278406 1106 34-35 0.3746084015389215 1106 36-37 0.25110939037210755 1106 38-39 0.1454184530521303 1106 40-41 0.22490666950324822 1106 42-43 -0.08160464734051942 1106 44-45 0.0598166232196391 1106 46-47 0.14416383582990733 1106 48-49 0.34310833413599084 1106 50-51 0.1547479182709921 1106 52-53 0.29893993373377725 1106 54-55 0.06830011593389429 1106 56-57 -0.16625897330481365 1106 58-59 -0.06145729464185479 1106 60-61 0.25777513313300204 1106 62-63 0.03428441378371616 1106 64-65 -0.05238273047746844 1106 66-67 -0.09866949815807757 1106 68-69 -0.045306563140460554 1106 70-71 -0.19545576251212182 1106 72-73 -0.14500552441938552 1106 74-75 -0.0947871298437839 1106 76-77 -0.3355642894025479 1106 78-79 -0.19081800699812135 1106 80-81 -0.11020879171830344 1106 82-83 -0.061988676110242835 1106 84-85 -0.2928014822497964 1106 86-87 -0.3592976497964031 1106 88-89 -0.5806813912837292 1106 90-91 -0.5018344278976379 1106 92-93 -0.5411720887270874 1106 94-95 -0.7364951995185933 1106 96-97 -0.8031846375655043 1106 98-99 -0.7939318394489199 1106 100-101 -1.3233878735736528 1107 1 -0.10208370072469819 1107 2 -0.07408543719575533 1107 3 -0.08223086996406437 1107 4 0.04324307375846104 1107 5 -0.02360806884475153 1107 6 -0.09013473220237955 1107 7 -0.04497747377862282 1107 8 0.043126765023529856 1107 9 0.029644451206060296 1107 10-11 -0.07908392327921376 1107 12-13 -0.09931288492011703 1107 14-15 -0.21139316100735073 1107 16-17 -0.03115696038600646 1107 18-19 -0.00808535367091423 1107 20-21 -0.09962372885091497 1107 22-23 0.07395532790717851 1107 24-25 0.14137085710549258 1107 26-27 -0.04354648192849453 1107 28-29 -0.04734910316127383 1107 30-31 -0.02511139523267758 1107 32-33 0.06194110525382257 1107 34-35 0.057478824837843945 1107 36-37 0.06013893974391493 1107 38-39 0.093345728867682 1107 40-41 0.1042252668036312 1107 42-43 0.09231113711314975 1107 44-45 0.04686265508491516 1107 46-47 0.09473410310152275 1107 48-49 0.12746891072927724 1107 50-51 0.16267950579708668 1107 52-53 0.17747828563686596 1107 54-55 0.011383032522985559 1107 56-57 -0.08051668947013013 1107 58-59 -0.036439574164823796 1107 60-61 -0.06254314949962492 1107 62-63 -0.0803462217907267 1107 64-65 -0.20769653263310772 1107 66-67 -0.17603247341255823 1107 68-69 -0.29860322515787985 1107 70-71 -0.3997942579211262 1107 72-73 -0.49390206405391623 1107 74-75 -0.5105939042378829 1107 76-77 -0.6057304100280945 1107 78-79 -0.7286948597542953 1107 80-81 -0.7920548571191155 1107 82-83 -0.9001099680119573 1107 84-85 -0.8083560869062261 1107 86-87 -0.7440653346147172 1107 88-89 -0.8116670200040765 1107 90-91 -0.8443705411954561 1107 92-93 -0.9587895637850394 1107 94-95 -1.2187559948549769 1107 96-97 -1.296882571755301 1107 98-99 -1.5748642795177723 1107 100-101 -1.4643644760110757 1108 1 -0.1266064627772323 1108 2 -0.04916679485231157 1108 3 -0.012662890294436124 1108 4 0.029856041179215254 1108 5 -0.00774359186434026 1108 6 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0.40334229960966894 2316 92-93 0.27394107204572293 2316 94-95 0.14696775543124474 2316 96-97 0.23457689332380127 2316 98-99 0.17585435318305187 2316 100-101 0.03727469044033249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6158.0 3 1688.0 4 1582.0 5 1926.0 6 2301.0 7 2761.0 8 3487.0 9 4692.0 10 5766.0 11 7406.0 12 9520.0 13 12064.0 14 14445.0 15 17837.0 16 20979.0 17 26596.0 18 26021.0 19 22207.0 20 22234.0 21 23731.0 22 25878.0 23 28260.0 24 31783.0 25 35681.0 26 40336.0 27 46802.0 28 53989.0 29 63142.0 30 73174.0 31 89097.0 32 107834.0 33 136607.0 34 175994.0 35 236855.0 36 306264.0 37 337910.0 38 185055.0 39 12563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86001940116163 18.437281979333388 14.828584738925397 22.87411388057958 2 15.523592739475564 19.472131434127494 40.76101599762825 24.2432598287687 3 19.56524288273401 23.706119739284638 29.587369051506258 27.141268326475092 4 12.910895499910271 16.448670696525983 36.63369372407879 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25.941608112316043 29.55922902310133 22.13501121445559 26-27 21.832697442003102 26.198677706822526 29.523938377169245 22.444686474005124 28-29 22.022131156965376 26.66783447826213 29.321336887268078 21.98869747750441 30-31 22.103765662462962 26.15462840796758 29.20206126734946 22.539544662220003 32-33 21.784431521110832 26.17141883233703 29.29602149092867 22.74812815562347 34-35 22.582344175530547 26.322212576299698 28.97155083896989 22.123892409199865 36-37 22.643223846719494 25.829499074572155 29.09597880023176 22.431298278476596 38-39 22.12499448067425 26.310378044527667 28.96533142537376 22.59929604942432 40-41 22.66335358163297 26.15777362127644 29.018272134083066 22.160600663007525 42-43 22.28759049062926 26.123440134299347 28.82907361883435 22.759895756237043 44-45 22.318154961883817 26.416407606973202 28.902682380013932 22.362755051129046 46-47 22.84277521861055 26.700381892630322 28.157507731568586 22.29933515719054 48-49 22.45887204443326 26.813019033977064 28.088716051824385 22.639392869765285 50-51 22.58751842238179 27.02396137416212 28.046423275707866 22.342096927748216 52-53 22.933124866555136 26.663950472473648 27.972588510543606 22.43033615042761 54-55 22.50527260529554 26.992787986074283 27.984254047437002 22.517685361193173 56-57 22.52214147299916 27.131986692143663 27.8506893112833 22.495182523573877 58-59 22.559056891960694 26.718518385019845 27.81787994431103 22.90454477870843 60-61 22.203158664139373 26.885180542556817 28.20160219482446 22.710058598479346 62-63 22.301495884240126 26.864099386493944 28.368296472582365 22.46610825668356 64-65 22.159394487097124 26.96201083190899 28.099442366621645 22.779152314372237 66-67 22.102866966355002 26.979000567856616 27.9613632252814 22.956769240506986 68-69 22.546706862055913 26.628228699147428 28.18627318444817 22.638791254348494 70-71 22.459938466168897 26.718848244560146 27.77141603281384 23.049797256457122 72-73 22.439703191604877 26.751661088030122 27.81995234016632 22.988683380198687 74-75 22.326112381580536 27.279118528490052 27.584076242582174 22.81069284734724 76-77 22.494330350808838 26.838283813517528 27.712051636034424 22.955334199639214 78-79 22.606309185776677 27.02046909007048 27.649554440104357 22.72366728404849 80-81 22.75095346393323 27.059119493455817 27.323684057614585 22.866242984996372 82-83 22.67535885453017 27.058274548010058 27.46946214779197 22.796904449667803 84-85 22.567785195299418 27.20136780870676 27.442427878470955 22.788419117522864 86-87 22.782927134136386 27.057179438959768 27.229651283910773 22.93024214299308 88-89 22.62230281313143 27.148737091985993 27.120325491942012 23.108634602940565 90-91 22.61258426378707 27.12542202334072 27.145055209601733 23.11693850327047 92-93 22.764152344356493 26.775886464411286 27.239937651783364 23.22002353944886 94-95 22.616729095390774 26.617800782854623 27.386211510455148 23.379258611299452 96-97 22.837239651984568 26.27760300698888 27.435709209646987 23.449448131379565 98-99 22.606712107950646 26.469153658901995 27.09208449780848 23.832049735338877 100-101 23.492867906798057 25.36454073143523 27.17822612572721 23.9643652360395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 591.0 1 419.0 2 357.5 3 479.5 4 423.0 5 406.5 6 720.0 7 1589.0 8 2702.0 9 3247.0 10 2814.5 11 2199.0 12 2105.0 13 2188.0 14 2425.0 15 2669.0 16 2781.0 17 2967.5 18 3206.5 19 3542.5 20 3998.5 21 4689.5 22 5702.5 23 6950.5 24 8541.0 25 10762.5 26 13851.5 27 17256.5 28 20084.5 29 22861.5 30 25889.0 31 30470.0 32 36890.0 33 40051.5 34 40465.5 35 43207.0 36 47788.5 37 51923.0 38 55250.5 39 57894.0 40 60864.5 41 65014.5 42 67615.5 43 69337.0 44 72155.0 45 75676.0 46 77610.0 47 79793.0 48 81854.0 49 80508.0 50 78332.5 51 77007.0 52 76542.5 53 77118.0 54 78775.0 55 75582.0 56 69428.0 57 64161.0 58 59624.5 59 53138.5 60 43092.0 61 35625.0 62 30151.0 63 23007.5 64 16473.0 65 12522.5 66 9495.0 67 7248.0 68 5605.0 69 4390.0 70 3387.5 71 2306.5 72 1568.5 73 1131.0 74 760.5 75 508.0 76 385.5 77 276.5 78 176.5 79 103.0 80 67.5 81 37.0 82 19.5 83 17.5 84 14.0 85 14.0 86 8.5 87 7.0 88 7.0 89 4.5 90 3.5 91 3.0 92 3.0 93 2.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14554461018857304 2 0.05273290177314945 3 0.15045313819307626 4 0.12820714888826343 5 0.18976639459611597 6 0.195575569940895 7 0.165178722206586 8 0.07119617224880383 9 0.12176752040529132 10-11 0.10163805235012666 12-13 0.15137630171685898 14-15 0.13642555586828034 16-17 0.13426400225161836 18-19 0.1325077399380805 20-21 0.13376864621446666 22-23 0.19519279482127777 24-25 0.21322825781030116 26-27 0.14703067830002814 28-29 0.12615817618913594 30-31 0.09835068955811989 32-33 0.12424430059104982 34-35 0.0932620320855615 36-37 0.08652969321699971 38-39 0.05183225443287363 40-41 0.06052350126653532 42-43 0.0632029271038559 44-45 0.04046158176189136 46-47 0.05192231916690121 48-49 0.04217281170841542 50-51 0.053048128342245995 52-53 0.027334646777371234 54-55 0.05106670419363918 56-57 0.02625386996904025 58-59 0.052012383900928785 60-61 0.03861525471432592 62-63 0.05262032085561497 64-65 0.03994370954123276 66-67 0.03938080495356037 68-69 0.06597241767520405 70-71 0.05383619476498734 72-73 0.051607092597804674 74-75 0.043816493104418805 76-77 0.021165212496481848 78-79 0.022381086405854206 80-81 0.025308190261750633 82-83 0.021187728679988742 84-85 0.019431466366450885 86-87 0.024880382775119617 88-89 0.06579228820714889 90-91 0.10996904024767802 92-93 0.08090064734027583 94-95 0.07630734590486912 96-97 0.0688094567970729 98-99 0.057528848860118206 100-101 0.13072896144103577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.173135837037194 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4718455655759 42.74364658935486 2 12.762635666858452 14.083092625723943 3 4.159717521632375 6.8851397959418 4 1.8259769264252899 4.029794919878327 5 1.0045064433681168 2.7710885224564104 6 0.5905918349836909 1.9550882121480138 7 0.3914206008054432 1.5117131384357396 8 0.27960022789294764 1.2341137082883324 9 0.21265817756684116 1.055971666597689 >10 1.1649399373360732 11.797089019842886 >50 0.0865124011954392 3.2842504236268653 >100 0.04369635049295233 4.433424237297916 >500 0.0033587802851507667 1.2854494389627573 >1k 0.0023757226407163465 2.1491503295794363 >5k 1.6384294073905838E-4 0.7809873718648779 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9473 0.4265916127216437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7806 0.35152265690965384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4633 0.20863495637489446 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2700 0.12158739093723614 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2418 0.10888826343934703 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2406 0.10834787503518153 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2359 0.10623135378553335 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.125809175344779E-4 14-15 0.0 0.0 0.0 0.0 2.251618350689558E-4 16-17 0.0 0.0 0.0 4.503236701379116E-5 3.152265690965381E-4 18-19 0.0 0.0 0.0 4.503236701379116E-5 4.052913031241205E-4 20-21 0.0 0.0 0.0 9.006473402758233E-5 5.178722206585983E-4 22-23 0.0 0.0 0.0 1.5761328454826909E-4 5.40388404165494E-4 24-25 0.0 0.0 0.0 3.602589361103293E-4 5.40388404165494E-4 26-27 0.0 0.0 0.0 0.0010357444413171967 5.40388404165494E-4 28-29 0.0 0.0 0.0 0.003827751196172249 5.40388404165494E-4 30-31 0.0 0.0 0.0 0.012158739093723614 5.629045876723896E-4 32-33 0.0 0.0 0.0 0.030554461018857303 5.854207711792852E-4 34-35 0.0 0.0 0.0 0.057573881227132 6.304531381930762E-4 36-37 0.0 0.0 0.0 0.10208837602026456 6.304531381930762E-4 38-39 0.0 0.0 0.0 0.16623698283141009 6.304531381930762E-4 40-41 0.0 0.0 0.0 0.2358570222347312 6.304531381930762E-4 42-43 0.0 0.0 0.0 0.3070531944835351 6.754855052068674E-4 44-45 0.0 0.0 0.0 0.3914438502673797 7.430340557275541E-4 46-47 0.0 4.503236701379116E-5 0.0 0.48970447509147197 7.655502392344498E-4 48-49 0.0 4.503236701379116E-5 0.0 0.5925358851674641 8.330987897551365E-4 50-51 0.0 4.503236701379116E-5 0.0 0.7019420208274698 9.006473402758232E-4 52-53 0.0 4.503236701379116E-5 0.0 0.8040529130312413 9.456797072896144E-4 54-55 0.0 4.503236701379116E-5 0.0 0.913414016324233 9.456797072896144E-4 56-57 0.0 4.503236701379116E-5 0.0 1.0419589079651 9.456797072896144E-4 58-59 0.0 4.503236701379116E-5 0.0 1.1791725302561216 9.681958907965099E-4 60-61 4.503236701379116E-5 4.503236701379116E-5 0.0 1.3271263720799324 9.907120743034056E-4 62-63 4.503236701379116E-5 4.503236701379116E-5 0.0 1.463056571911061 0.0010357444413171967 64-65 4.503236701379116E-5 4.503236701379116E-5 0.0 1.606214466647903 0.0010357444413171967 66-67 4.503236701379116E-5 4.503236701379116E-5 0.0 1.7743202927103856 0.0010357444413171967 68-69 4.503236701379116E-5 4.503236701379116E-5 0.0 1.9623529411764706 0.0010582606248240923 70-71 4.503236701379116E-5 4.503236701379116E-5 0.0 2.157230509428652 0.001080776808330988 72-73 4.503236701379116E-5 4.503236701379116E-5 0.0 2.354539825499578 0.0011258091753447792 74-75 4.503236701379116E-5 4.503236701379116E-5 0.0 2.547751196172249 0.0011708415423585703 76-77 4.503236701379116E-5 4.503236701379116E-5 0.0 2.759560934421615 0.0011708415423585703 78-79 4.503236701379116E-5 4.503236701379116E-5 0.0 2.9938868561778778 0.0011708415423585703 80-81 4.503236701379116E-5 4.503236701379116E-5 0.0 3.2446946242611876 0.0011708415423585703 82-83 4.503236701379116E-5 4.503236701379116E-5 0.0 3.50063608218407 0.0013059386433999438 84-85 4.503236701379116E-5 4.503236701379116E-5 0.0 3.7579510273008725 0.0013284548269068392 86-87 4.503236701379116E-5 4.503236701379116E-5 0.0 4.014928229665072 0.0013734871939206305 88-89 4.503236701379116E-5 4.503236701379116E-5 0.0 4.300838727835632 0.0013960033774275261 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5820 0.0 44.98052 1 GTATCAA 9825 0.0 37.835823 1 TATCAAC 11890 0.0 30.711649 2 ATCAACG 11940 0.0 30.504086 3 TCAACGC 12225 0.0 29.79295 4 CAACGCA 12235 0.0 29.768595 5 AACGCAG 12575 0.0 28.998259 6 ACGCAGA 14090 0.0 25.643505 7 CGCAGAG 14270 0.0 25.249989 8 TAAGGTG 1285 0.0 23.311543 5 TACATGG 8065 0.0 22.933428 2 GAGTACT 9195 0.0 22.643484 12-13 GCAGAGT 15605 0.0 22.601969 9 GTACATG 8365 0.0 22.475836 1 GTAAGGT 1290 0.0 21.746824 4 ACATGGG 8285 0.0 21.693663 3 GTACTTT 9590 0.0 21.683107 14-15 TGGTATC 3115 0.0 21.064192 2 AGAGTAC 13100 0.0 20.700726 10-11 GTGTAAG 955 0.0 20.434555 1 >>END_MODULE