FastQCFastQC Report
Thu 26 May 2016
SRR938228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220625
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92410.41614410357444415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.3530087250211089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.21534477905994936No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26380.11879538418238109No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25110.11307627357162961No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23810.10722206585983675No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23700.10672670982268505No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22740.10240360258936111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.042.085961
GTATCAA94250.036.4405331
TATCAAC115250.029.7567122
ATCAACG115950.029.4515173
TCAACGC118300.028.8638734
CAACGCA119900.028.5579155
AACGCAG123900.027.6359486
ACGACCG700.00840107127.1376575
GTAAGGT13200.026.263914
TAAGGTG13850.025.7170965
GTGTAAG8250.024.7595231
ACGCAGA139750.024.4670337
CGCAGAG139400.024.3921978
GTACATG83450.022.826841
TACATGG80750.022.8232692
GGTAAGG14750.022.2180843
GAGTACT89250.022.19052512-13
ACATGGG80850.021.50063
GCAGAGT154950.021.4232949
GTACTTT93250.021.03492414-15
Produced by FastQC (version 0.11.5)