##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938228_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220625 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57437703349282 34.0 31.0 34.0 31.0 34.0 2 32.78566574725584 34.0 31.0 34.0 31.0 34.0 3 32.83804694624261 34.0 31.0 34.0 31.0 34.0 4 36.27466456515621 37.0 37.0 37.0 35.0 37.0 5 36.24952524627076 37.0 37.0 37.0 35.0 37.0 6 36.28233267661131 37.0 37.0 37.0 35.0 37.0 7 36.24483962848297 37.0 37.0 37.0 35.0 37.0 8 36.2293836194765 37.0 37.0 37.0 35.0 37.0 9 37.931091471995494 39.0 38.0 39.0 35.0 39.0 10-11 37.90969479313256 39.0 38.0 39.0 35.0 39.0 12-13 37.83200810582606 39.0 38.0 39.0 35.0 39.0 14-15 39.136294511680276 40.0 38.5 41.0 36.0 41.0 16-17 39.030168983957225 40.0 38.0 41.0 36.0 41.0 18-19 39.27407306501548 40.5 39.0 41.0 36.0 41.0 20-21 39.25348088938925 41.0 39.0 41.0 36.0 41.0 22-23 39.16701469180974 40.5 39.0 41.0 36.0 41.0 24-25 39.07596059667886 40.5 39.0 41.0 35.0 41.0 26-27 38.910789755136506 40.0 38.0 41.0 35.0 41.0 28-29 38.80092338868562 40.0 38.0 41.0 35.0 41.0 30-31 38.564640585420776 40.0 38.0 41.0 34.5 41.0 32-33 38.357400956937795 40.0 38.0 41.0 34.0 41.0 34-35 38.2119871657754 40.0 38.0 41.0 34.0 41.0 36-37 38.212421728117086 40.0 38.0 41.0 34.0 41.0 38-39 38.07018609625669 40.0 38.0 41.0 33.5 41.0 40-41 37.90225116802702 40.0 38.0 41.0 33.0 41.0 42-43 37.6732591049817 40.0 37.0 41.0 33.0 41.0 44-45 37.40959977483817 40.0 37.0 41.0 32.5 41.0 46-47 37.033389698846044 40.0 36.5 41.0 31.5 41.0 48-49 36.638894680551644 39.5 36.0 41.0 30.5 41.0 50-51 36.268034224598935 39.0 35.5 41.0 30.0 41.0 52-53 35.7721386996904 39.0 35.0 40.0 28.5 41.0 54-55 35.4720950182944 38.5 35.0 40.0 28.0 41.0 56-57 35.13511286236983 38.0 34.5 40.0 27.0 41.0 58-59 34.7078658035463 38.0 34.0 40.0 26.0 41.0 60-61 34.25070059104982 37.0 34.0 40.0 25.5 41.0 62-63 33.80786220095694 37.0 33.0 39.5 24.5 41.0 64-65 33.33199144385027 36.0 33.0 39.0 23.0 40.5 66-67 32.802035462989025 35.5 32.0 39.0 22.0 40.0 68-69 32.23872085561497 35.0 31.0 38.0 19.5 40.0 70-71 31.6465040247678 35.0 31.0 37.5 17.5 40.0 72-73 31.086654207711792 35.0 30.0 37.0 13.5 39.0 74-75 30.514627638615252 34.0 30.0 36.5 8.0 39.0 76-77 29.166552659724175 32.5 28.5 35.0 7.5 37.0 78-79 29.85000776808331 34.0 29.5 35.0 7.0 37.5 80-81 29.549661919504643 34.0 29.0 35.0 2.0 37.0 82-83 29.011292991837884 34.0 29.0 35.0 2.0 36.5 84-85 28.444380298339432 34.0 28.0 35.0 2.0 36.0 86-87 27.839569715733184 33.0 26.5 35.0 2.0 36.0 88-89 27.130314888826344 33.0 25.5 35.0 2.0 35.0 90-91 26.61400911905432 32.5 25.0 35.0 2.0 35.0 92-93 25.850246439628485 32.0 23.5 34.0 2.0 35.0 94-95 25.073202814522936 32.0 19.5 34.0 2.0 35.0 96-97 24.07976898395722 31.0 8.5 34.0 2.0 35.0 98-99 22.71373059386434 31.0 2.0 34.0 2.0 35.0 100-101 18.304236645088658 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09319208000415813 1101 2 0.09813047756317417 1101 3 0.08806455452636186 1101 4 0.12740782891042102 1101 5 0.131372711011295 1101 6 0.10024903473234303 1101 7 0.08692592940398924 1101 8 0.11709630141721306 1101 9 0.020294378102455823 1101 10-11 0.12451237404492588 1101 12-13 0.15552569586584752 1101 14-15 0.14494167100856714 1101 16-17 0.11366863898015112 1101 18-19 0.17408594110990805 1101 20-21 0.20335184865044909 1101 22-23 0.26542524458587735 1101 24-25 0.23244078006115387 1101 26-27 0.0945050420400051 1101 28-29 0.14251446889715425 1101 30-31 0.1842698448265807 1101 32-33 0.09945457921586609 1101 34-35 0.0055906620622678815 1101 36-37 0.007896201147708837 1101 38-39 0.0826388999895471 1101 40-41 -0.03565622462426887 1101 42-43 -0.02577975650431341 1101 44-45 -0.05010304064094839 1101 46-47 -0.07270818112769462 1101 48-49 0.10227520921529987 1101 50-51 0.03706742369694638 1101 52-53 -0.1910834379553208 1101 54-55 -0.256507013607397 1101 56-57 -0.15143981960697062 1101 58-59 -0.1392649717362744 1101 60-61 -0.2522486710942644 1101 62-63 -0.12784482037205436 1101 64-65 -0.20518013720303685 1101 66-67 -0.35779519446352026 1101 68-69 -0.2925679014882725 1101 70-71 -0.19010944141954056 1101 72-73 -0.2087389031980713 1101 74-75 -0.3383839765648311 1101 76-77 -0.34618916543065126 1101 78-79 -0.45469424549796145 1101 80-81 -0.3160374643836974 1101 82-83 -0.4560843935769405 1101 84-85 -0.36226040585127706 1101 86-87 -0.7940919214442204 1101 88-89 -0.7949293623781841 1101 90-91 -0.5274791522394331 1101 92-93 -0.6908309763745528 1101 94-95 -0.8912641098388185 1101 96-97 -0.9401053312585468 1101 98-99 -0.924210163335804 1101 100-101 -1.1403834565188937 1102 1 0.13615035208376725 1102 2 0.17447221679194058 1102 3 0.08889402416335912 1102 4 0.04128264514925206 1102 5 0.08085026387797001 1102 6 0.029329098059264425 1102 7 0.07943921674991117 1102 8 0.11561999247228272 1102 9 0.11731744719665471 1102 10-11 0.1760367436027721 1102 12-13 0.1640575854960602 1102 14-15 0.19320526933492488 1102 16-17 0.17957217850293006 1102 18-19 0.16145024055579427 1102 20-21 0.18103023833720044 1102 22-23 0.19005715683285018 1102 24-25 0.1811787649079264 1102 26-27 0.029207631658913158 1102 28-29 0.053684038047897786 1102 30-31 0.07569526606371113 1102 32-33 0.02681718240481956 1102 34-35 0.14635115058426607 1102 36-37 0.22807430812497387 1102 38-39 0.12263437663774113 1102 40-41 0.25371813927938547 1102 42-43 0.22891237286354738 1102 44-45 0.07017175298112477 1102 46-47 0.165044843863825 1102 48-49 0.1214222746908149 1102 50-51 0.22102224672080695 1102 52-53 0.21931998848366874 1102 54-55 0.21708537585320187 1102 56-57 0.2875534519072218 1102 58-59 0.2526780905728927 1102 60-61 0.16439245748200904 1102 62-63 0.22566925856043696 1102 64-65 0.16093260734066206 1102 66-67 0.07685452848118501 1102 68-69 0.147870015508218 1102 70-71 -0.16157161489008232 1102 72-73 -0.018083582605513016 1102 74-75 0.0302448824196766 1102 76-77 -0.005080378819769749 1102 78-79 -0.07235765158187135 1102 80-81 -0.1940466241710972 1102 82-83 -0.06551445124967614 1102 84-85 -0.14006092603673537 1102 86-87 -0.23493665739355762 1102 88-89 -0.39093609086399184 1102 90-91 -0.5173290335014435 1102 92-93 -0.6212906620016021 1102 94-95 -0.7751974469416147 1102 96-97 -0.7683409413456168 1102 98-99 -0.7773346685941966 1102 100-101 -1.5392823030641836 1103 1 0.08202553358093922 1103 2 0.09475737139745632 1103 3 0.0617134806598898 1103 4 0.02341537649907366 1103 5 0.009769958361310671 1103 6 0.01971404388934417 1103 7 0.03369656040460001 1103 8 0.03184240275469108 1103 9 0.09595340468550972 1103 10-11 0.10705385803404965 1103 12-13 0.12776083655068504 1103 14-15 0.19146117819542496 1103 16-17 0.12143294332556565 1103 18-19 0.13436259353234448 1103 20-21 0.05027065657541385 1103 22-23 0.10412522793678392 1103 24-25 0.13402494122436792 1103 26-27 0.11247080040617874 1103 28-29 0.1072616801741404 1103 30-31 0.09607127664853721 1103 32-33 0.13420068055334156 1103 34-35 0.11529902545646564 1103 36-37 0.09474369545926464 1103 38-39 0.08868474049123876 1103 40-41 0.16400108155254856 1103 42-43 0.12631331192346096 1103 44-45 0.13004541376828627 1103 46-47 0.1676062092380306 1103 48-49 0.16223497390812724 1103 50-51 -0.0806652473724796 1103 52-53 -0.14659817240228534 1103 54-55 -0.0613618890166876 1103 56-57 -0.06693790496132124 1103 58-59 -0.053910901033127345 1103 60-61 -0.1596181144596045 1103 62-63 -0.19988683158243958 1103 64-65 -0.22333985749828145 1103 66-67 -0.084653788170165 1103 68-69 -0.1536123545655812 1103 70-71 -0.22768061629050962 1103 72-73 -0.3040704976261779 1103 74-75 -0.36635972438657305 1103 76-77 -0.30958752272017875 1103 78-79 -0.28133445860634865 1103 80-81 -0.3865882718646887 1103 82-83 -0.3897596807737891 1103 84-85 -0.5319476803948184 1103 86-87 -0.552443938759275 1103 88-89 -0.6194007536873549 1103 90-91 -1.1596834921029107 1103 92-93 -1.098758164342808 1103 94-95 -1.1043436370045896 1103 96-97 -1.1849344005742672 1103 98-99 -1.4349246868942664 1103 100-101 -1.7216005051897412 1104 1 0.09895846192100777 1104 2 0.10006237526448558 1104 3 0.13564347687812273 1104 4 0.08609437085050331 1104 5 -0.028940469780636136 1104 6 0.026403855894493233 1104 7 0.03234585837056869 1104 8 0.0186511146574162 1104 9 0.11105739319376084 1104 10-11 0.039717750403212904 1104 12-13 0.10072209402267873 1104 14-15 0.1239011238664034 1104 16-17 0.06021110719335354 1104 18-19 0.023504338500941913 1104 20-21 0.1450269433836766 1104 22-23 0.10866597921400256 1104 24-25 0.04129909612024818 1104 26-27 0.1594530873006974 1104 28-29 -0.010063067114671753 1104 30-31 -0.05505863851754356 1104 32-33 0.010726050980743196 1104 34-35 0.15003421162062835 1104 36-37 -0.022343437611716865 1104 38-39 0.07915509376026364 1104 40-41 0.07204588393275202 1104 42-43 0.004444302434940539 1104 44-45 -0.09384774284192332 1104 46-47 -0.19715067442704992 1104 48-49 -0.1777292798854262 1104 50-51 0.12034948445825222 1104 52-53 0.028598364022528244 1104 54-55 0.01609257181095103 1104 56-57 -0.028486783522524206 1104 58-59 0.06441107363004761 1104 60-61 -0.24206686251480392 1104 62-63 -0.2142732126138256 1104 64-65 -0.32605339932691635 1104 66-67 -0.4568866796956925 1104 68-69 -0.4218435718826541 1104 70-71 -0.4782796047435873 1104 72-73 -0.6014418699655231 1104 74-75 -0.5568687730369284 1104 76-77 -0.5432091947418343 1104 78-79 -0.681884068442546 1104 80-81 -0.557452455339476 1104 82-83 -0.7153612522626922 1104 84-85 -0.6904310973048204 1104 86-87 -0.745325367885286 1104 88-89 -0.797169291608828 1104 90-91 -1.0608191752130658 1104 92-93 -0.9226585685001432 1104 94-95 -1.0522066889592203 1104 96-97 -1.0061109558376877 1104 98-99 -0.9775398877842356 1104 100-101 -1.2772937669270057 1105 1 0.11112103168311194 1105 2 0.14084290590059823 1105 3 0.07535314993995712 1105 4 0.05094847182606088 1105 5 0.04285459061816965 1105 6 0.040140005248311184 1105 7 0.08263078198383766 1105 8 0.09699955694546958 1105 9 0.1809823387904146 1105 10-11 0.08273744570749386 1105 12-13 0.09591241781506454 1105 14-15 0.08801932252372069 1105 16-17 0.06894709925860809 1105 18-19 0.13320756273152767 1105 20-21 0.10295872825732744 1105 22-23 0.18238828717391442 1105 24-25 0.0582945884149666 1105 26-27 0.2168829597304196 1105 28-29 0.16779190542459332 1105 30-31 0.14527772770946257 1105 32-33 0.11768169838047982 1105 34-35 0.11689108180019048 1105 36-37 0.2016958530723585 1105 38-39 0.18788613586178826 1105 40-41 0.16133906612102322 1105 42-43 0.12184437330731868 1105 44-45 0.19739158466617823 1105 46-47 0.27665453386001815 1105 48-49 0.259056791934384 1105 50-51 0.07906297037999366 1105 52-53 0.11833993428055578 1105 54-55 0.1685393111264304 1105 56-57 0.21335005549540398 1105 58-59 0.3020116457903441 1105 60-61 0.20978756969197576 1105 62-63 0.20893087577864122 1105 64-65 0.39971953519663117 1105 66-67 0.23203076786244736 1105 68-69 0.020166793433915586 1105 70-71 -0.13037510481020576 1105 72-73 -0.09116468124884847 1105 74-75 -0.020275356581212378 1105 76-77 -0.11077249876398554 1105 78-79 -0.02497449734071111 1105 80-81 -0.12657800492423732 1105 82-83 -0.11465088620593278 1105 84-85 -0.1912904017562731 1105 86-87 -0.2981052813950775 1105 88-89 -0.3372985781848996 1105 90-91 -0.4436139802492569 1105 92-93 -0.4313093240403987 1105 94-95 -0.3635202011574101 1105 96-97 -0.3922731240513393 1105 98-99 -0.527953764891901 1105 100-101 -1.4426804978784347 1106 1 0.12934715538802521 1106 2 0.1665831976354255 1106 3 0.1834345260402941 1106 4 0.09227408333965315 1106 5 0.08628690541134887 1106 6 0.12508610830977318 1106 7 0.08812929214976606 1106 8 0.10117328328339426 1106 9 0.07649530911058378 1106 10-11 0.06546839433256935 1106 12-13 0.09688677416780678 1106 14-15 0.13854064273093059 1106 16-17 0.16953329541035345 1106 18-19 0.2517486938369302 1106 20-21 0.28985987338469243 1106 22-23 0.26397106547102567 1106 24-25 0.2811825295226953 1106 26-27 0.2734948287418746 1106 28-29 0.27375951857408154 1106 30-31 0.21308246207106407 1106 32-33 0.28463695953963253 1106 34-35 0.4022258319365122 1106 36-37 0.3274098536176595 1106 38-39 0.191808635277539 1106 40-41 0.2996282886808146 1106 42-43 0.25408406618726787 1106 44-45 0.22914659459840436 1106 46-47 0.12041694914396572 1106 48-49 0.11673223693711066 1106 50-51 0.2754541394615515 1106 52-53 0.2130477369213608 1106 54-55 0.11982453645166657 1106 56-57 0.21705041870331598 1106 58-59 0.20809416117555912 1106 60-61 0.14111060077400595 1106 62-63 0.12711245052351927 1106 64-65 0.07587732128371982 1106 66-67 -0.11174016771440876 1106 68-69 -0.23746264198712908 1106 70-71 -0.6310696304002938 1106 72-73 -0.39349607682414245 1106 74-75 -0.13877583371761304 1106 76-77 -0.3194539469216551 1106 78-79 -0.30631030886209487 1106 80-81 -0.40344175813760685 1106 82-83 -0.3935533020741282 1106 84-85 -0.48131411251424083 1106 86-87 -0.42049989754371 1106 88-89 -0.6271846253532409 1106 90-91 -0.811539459333158 1106 92-93 -0.8786674246480182 1106 94-95 -0.8677186363447582 1106 96-97 -0.892623916682016 1106 98-99 -0.794187235523669 1106 100-101 -1.2669692968579476 1107 1 -0.003919542418771016 1107 2 0.0016317424875609277 1107 3 0.07940514543493293 1107 4 0.020003499549289927 1107 5 0.0014520406586058243 1107 6 0.012683298729498915 1107 7 -0.026870560860103865 1107 8 -0.03669583888064665 1107 9 0.006075712168417624 1107 10-11 0.06766083381346277 1107 12-13 0.03601598284914331 1107 14-15 0.035068735811613294 1107 16-17 -0.07252319453061773 1107 18-19 0.05212433528552651 1107 20-21 -0.03245192208344605 1107 22-23 -0.0241768479768254 1107 24-25 0.058070781091409174 1107 26-27 0.11929183645830932 1107 28-29 0.03219234245050728 1107 30-31 0.05635221945024682 1107 32-33 0.010071587583823316 1107 34-35 -0.009295036851383998 1107 36-37 -0.08572148260974899 1107 38-39 -0.028550586828913538 1107 40-41 -0.167892336212347 1107 42-43 -0.10202642248413696 1107 44-45 -0.1878978367076627 1107 46-47 -0.029637219441738694 1107 48-49 -0.007302500641955589 1107 50-51 -0.14431929974822566 1107 52-53 -0.2784863936429005 1107 54-55 -0.3060799156072349 1107 56-57 -0.19937621852177045 1107 58-59 -0.19223313685183285 1107 60-61 -0.2318143613079826 1107 62-63 -0.20844511571186075 1107 64-65 -0.34287557088322984 1107 66-67 -0.3578314823397122 1107 68-69 -0.2889818122223886 1107 70-71 -0.5065392638722379 1107 72-73 -0.790273182076696 1107 74-75 -0.5860019121624376 1107 76-77 -0.6532200845327267 1107 78-79 -0.4637111531298608 1107 80-81 -0.4090854170377476 1107 82-83 -0.45902989307898423 1107 84-85 -0.5872470697705978 1107 86-87 -0.6228476021566998 1107 88-89 -0.5336789760324976 1107 90-91 -0.8203328445594522 1107 92-93 -0.8472531291499443 1107 94-95 -1.0193956848700125 1107 96-97 -1.144503376063195 1107 98-99 -1.3273126050405288 1107 100-101 -1.8655268336953057 1108 1 -0.0851776968078326 1108 2 -0.08538529952044627 1108 3 -0.11163529607801337 1108 4 -0.027433494825373828 1108 5 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-0.32476812482838113 2316 50-51 -0.18442355648446807 2316 52-53 0.08561031441892908 2316 54-55 -0.10786651846706974 2316 56-57 -0.06707658081109713 2316 58-59 -0.005145133694597348 2316 60-61 0.20829261067430593 2316 62-63 0.22010151471860695 2316 64-65 0.2261958599802938 2316 66-67 0.25408863657354885 2316 68-69 0.2832012615726285 2316 70-71 0.3301175596645045 2316 72-73 0.5196452196975372 2316 74-75 0.8039640485257387 2316 76-77 0.7416407968593717 2316 78-79 0.8040969042393655 2316 80-81 0.9005046142050048 2316 82-83 1.066971782242348 2316 84-85 1.1009698659014475 2316 86-87 1.3821242021184936 2316 88-89 1.4322278428215292 2316 90-91 1.4936362106380159 2316 92-93 1.5112158321755125 2316 94-95 2.0702853347034242 2316 96-97 2.3664276672867963 2316 98-99 2.763843649819144 2316 100-101 3.301972629426494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 18.0 5 31.0 6 61.0 7 197.0 8 438.0 9 927.0 10 1713.0 11 2644.0 12 4069.0 13 6230.0 14 8629.0 15 11724.0 16 15538.0 17 22031.0 18 22451.0 19 17506.0 20 16289.0 21 16701.0 22 18101.0 23 19496.0 24 21933.0 25 25213.0 26 28622.0 27 34181.0 28 40859.0 29 49527.0 30 59806.0 31 72822.0 32 90924.0 33 118901.0 34 162110.0 35 235356.0 36 346357.0 37 454200.0 38 277325.0 39 17687.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20279475888134 18.771172586358933 15.124691390272748 22.90134126448698 2 15.718289001845681 19.7124015568941 40.16915594973332 24.400153491526886 3 19.58988253213219 23.56495590732815 29.764276131230023 27.080885429309635 4 13.14783592359625 16.740609846601675 36.377702843885324 33.73385138591675 5 13.846501638973663 37.107138305599804 34.10091970762817 14.94544034779836 6 32.72241241260174 35.85361855585607 16.83738767584354 14.586581355698641 7 28.93122431747819 32.758975513650434 19.863596960315228 18.44620320855615 8 26.725899240078803 34.738148043906556 19.116419926822402 19.419532789192232 9 26.11751196172249 17.033312693498452 19.958525189980296 36.89065015479876 10-11 25.333633549113426 26.482454264002254 26.604334365325077 21.579577821559244 12-13 26.226341512621087 23.703992913655668 27.277449564511357 22.792216009211888 14-15 22.805246270757106 25.258722206585983 25.722240360258937 26.213791162397975 16-17 22.21688325870918 28.43122663187084 26.686046053438922 22.665844055981058 18-19 22.211584576414296 26.71133126934985 29.1683197298058 21.908764424430057 20-21 22.676813960033773 25.825882352941175 29.60950182943991 21.88780185758514 22-23 22.496729524345625 25.676532507739935 29.58061356600056 22.246124401913875 24-25 22.325884691777283 25.90750132342653 29.625569929047224 22.141044055748964 26-27 21.88030349981443 26.2253899592475 29.448048672390993 22.44625786854707 28-29 22.042394668027327 26.67389179377629 29.291375355411176 21.992338182785208 30-31 22.122922991081985 26.139205362969086 29.21671595601292 22.521155689936013 32-33 21.838401350971008 26.110869687587957 29.293937517590766 22.756791443850265 34-35 22.551013353984047 26.304734209487513 29.037591252299393 22.106661184229047 36-37 22.645581488346455 25.85278292650855 29.05390495953368 22.447730625611317 38-39 22.044252561952508 26.2991022133036 29.00284251084856 22.653802713895338 40-41 22.623818188409402 26.113957834074768 29.015220992490942 22.24700298502489 42-43 22.277518546547796 26.128368883275694 28.844100917718336 22.750011652458173 44-45 22.299645370109765 26.40184632704757 28.934218969884608 22.364289332958066 46-47 22.793328090061195 26.699866051133835 28.182310058413773 22.324495800391198 48-49 22.53754662861098 26.733766983824907 28.108301992407025 22.620384395157085 50-51 22.574709330799394 26.91418348609393 28.157449429132107 22.35365775397457 52-53 22.93999586117787 26.650028811876574 27.93966146776864 22.470313859176915 54-55 22.527605284235854 26.910989129022518 27.967214805444023 22.594190781297605 56-57 22.51434068864131 27.13434847152761 27.820102726892753 22.531208112938327 58-59 22.53630181489368 26.659624949748938 27.89774346004295 22.906329775314425 60-61 22.21337803712381 26.911103930683606 28.14967513569319 22.72584289649939 62-63 22.300141136855455 26.936195196302293 28.288492110231328 22.475171556610924 64-65 22.11125396903684 27.03485275461685 28.01075330568211 22.843139970664204 66-67 22.03079447992711 27.0345033830511 27.923424098997764 23.011278038024027 68-69 22.491065562453446 26.721935888273528 28.094889841825488 22.692108707447538 70-71 22.420114995851826 26.80107771453176 27.651273232702206 23.127534056914207 72-73 22.3454749380265 26.87277739235503 27.692050301158904 23.089697368459568 74-75 22.271630961308496 27.32670346424172 27.45932336600976 22.94234220844002 76-77 22.386020037393887 26.881011139342448 27.748858297348512 22.984110525915153 78-79 22.431785985356086 27.11353649829845 27.706590211415588 22.748087304929875 80-81 22.61601936981447 27.05858324003023 27.389993163276976 22.93540422687832 82-83 22.499695922660745 27.10624236991121 27.557200326151097 22.836861381276943 84-85 22.408931222908297 27.246932206187815 27.483203058931682 22.860933511972206 86-87 22.5488436164153 27.198365343921722 27.306635181998963 22.946155857664017 88-89 22.444041464313287 27.31853957838429 27.00607983510846 23.231339122193965 90-91 22.345082665596397 27.296414839919937 27.19562648353141 23.16287601095226 92-93 22.471179044415397 26.950600111887557 27.295541704240282 23.28267913945676 94-95 22.256894667079223 26.81935753813781 27.46690429189169 23.45684350289127 96-97 22.454676108646726 26.476165125656614 27.492335484847295 23.576823280849364 98-99 22.152914360553908 26.757063134157548 27.14222468627934 23.9477978190092 100-101 22.630842825124578 26.169465770214924 27.179111176064858 24.020580228595644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 624.0 1 430.5 2 328.5 3 445.5 4 381.0 5 365.5 6 737.5 7 1663.0 8 2735.0 9 3218.0 10 2728.0 11 2080.5 12 2005.5 13 2153.0 14 2343.0 15 2506.5 16 2687.5 17 2900.0 18 3074.0 19 3398.0 20 3881.5 21 4520.5 22 5397.5 23 6605.5 24 8290.0 25 10438.5 26 13311.5 27 16768.0 28 19776.5 29 22409.0 30 25572.5 31 30634.5 32 37181.5 33 39897.5 34 40164.5 35 42861.0 36 47211.5 37 51656.0 38 55112.5 39 57906.5 40 61083.5 41 65478.5 42 68186.0 43 69869.5 44 72704.5 45 76342.5 46 78012.5 47 80284.5 48 82792.5 49 81013.0 50 79027.5 51 78136.0 52 77308.0 53 78274.0 54 80484.0 55 76817.5 56 70224.0 57 64850.5 58 60132.0 59 53363.5 60 43007.5 61 35467.5 62 29960.5 63 22064.5 64 15181.5 65 11375.5 66 8551.0 67 6697.5 68 5132.5 69 3896.5 70 2961.5 71 1917.0 72 1248.5 73 913.0 74 645.5 75 429.5 76 285.5 77 178.0 78 105.5 79 67.5 80 35.5 81 12.5 82 6.5 83 3.0 84 3.0 85 3.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008240923163523782 2 0.013824936673233887 3 0.008466084998592738 4 4.503236701379116E-5 5 0.0012609062763861525 6 0.0019363917815930199 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.205178722206586E-4 14-15 0.0 16-17 0.002364199268224036 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0028595553053757388 26-27 0.01769772023641993 28-29 0.00601182099634112 30-31 0.0028820714888826344 32-33 0.0 34-35 0.00907402195327892 36-37 5.854207711792852E-4 38-39 0.0023867154517309315 40-41 0.001823810864058542 42-43 0.0028595553053757388 44-45 0.0 46-47 6.754855052068675E-5 48-49 8.781311567689276E-4 50-51 0.004615817618913594 52-53 0.008398536448072053 54-55 0.007723050942865184 56-57 0.016616943428088938 58-59 0.023529411764705882 60-61 0.0037151702786377707 62-63 0.019791725302561215 64-65 0.021187728679988742 66-67 0.013149451168027019 68-69 0.03168027019420208 70-71 0.01670700816211652 72-73 0.005381367858148044 74-75 0.016661975795102733 76-77 0.021435406698564596 78-79 0.026569096538136786 80-81 0.011843512524627075 82-83 0.035800731775963976 84-85 0.02267379679144385 86-87 0.031905432029271034 88-89 0.022471151139881792 90-91 0.028212777934140166 92-93 0.024272445820433437 94-95 0.014500422178440756 96-97 0.0147706163805235 98-99 0.01161835068955812 100-101 0.010875316633830566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220625.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.12669586833915 #Duplication Level Percentage of deduplicated Percentage of total 1 75.68374048422568 40.20827062883765 2 13.70625491979245 14.563360732354813 3 4.511212833748544 7.1899749664772195 4 2.003675771138562 4.257946932481532 5 1.0547923579532017 2.8018816402614033 6 0.6373939966976473 2.031758220651665 7 0.43373533963757366 1.613004783339331 8 0.32004026050762824 1.3602145268490236 9 0.24181670082307985 1.156223008846163 >10 1.2569580888074283 12.263238424748632 >50 0.09627599880209349 3.520181167252417 >100 0.047716985407162606 4.677350440153207 >500 0.0034911568109282226 1.255973111140287 >1k 0.002724805315846343 2.327965432660602 >5k 1.7030033224039643E-4 0.7726559839459605 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9241 0.41614410357444415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7839 0.3530087250211089 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4782 0.21534477905994936 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2638 0.11879538418238109 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2511 0.11307627357162961 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2381 0.10722206585983675 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2370 0.10672670982268505 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2274 0.10240360258936111 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.503236701379116E-5 6 0.0 0.0 0.0 0.0 4.503236701379116E-5 7 0.0 0.0 0.0 0.0 4.503236701379116E-5 8 0.0 0.0 0.0 0.0 4.503236701379116E-5 9 0.0 0.0 0.0 0.0 4.503236701379116E-5 10-11 0.0 0.0 0.0 0.0 4.503236701379116E-5 12-13 0.0 0.0 0.0 0.0 2.70194202082747E-4 14-15 0.0 0.0 0.0 0.0 2.927103855896425E-4 16-17 0.0 0.0 0.0 4.503236701379116E-5 3.152265690965381E-4 18-19 0.0 0.0 0.0 4.503236701379116E-5 3.152265690965381E-4 20-21 0.0 0.0 0.0 9.006473402758233E-5 3.602589361103293E-4 22-23 0.0 0.0 0.0 1.5761328454826909E-4 4.052913031241205E-4 24-25 0.0 0.0 2.251618350689558E-5 3.602589361103293E-4 4.052913031241205E-4 26-27 0.0 0.0 4.503236701379116E-5 9.456797072896144E-4 4.052913031241205E-4 28-29 0.0 0.0 4.503236701379116E-5 0.0037376864621446663 4.052913031241205E-4 30-31 0.0 0.0 4.503236701379116E-5 0.012136222910216718 4.052913031241205E-4 32-33 0.0 0.0 4.503236701379116E-5 0.030531944835350405 4.052913031241205E-4 34-35 0.0 0.0 4.503236701379116E-5 0.05782155924570785 4.052913031241205E-4 36-37 0.0 0.0 4.503236701379116E-5 0.10258373205741628 4.052913031241205E-4 38-39 0.0 0.0 4.503236701379116E-5 0.16693498452012384 4.052913031241205E-4 40-41 0.0 0.0 4.503236701379116E-5 0.23657754010695187 4.052913031241205E-4 42-43 0.0 0.0 4.503236701379116E-5 0.30835913312693497 4.503236701379116E-4 44-45 0.0 0.0 4.503236701379116E-5 0.3929299183788348 4.503236701379116E-4 46-47 0.0 0.0 4.503236701379116E-5 0.49155080213903746 4.728398536448072E-4 48-49 0.0 0.0 4.503236701379116E-5 0.594719954967633 5.40388404165494E-4 50-51 0.0 0.0 4.503236701379116E-5 0.7039009287925697 5.40388404165494E-4 52-53 0.0 0.0 4.503236701379116E-5 0.8064171122994652 5.854207711792852E-4 54-55 0.0 0.0 4.503236701379116E-5 0.9166113143822121 5.854207711792852E-4 56-57 0.0 0.0 4.503236701379116E-5 1.0462144666479032 6.079369546861807E-4 58-59 0.0 0.0 4.503236701379116E-5 1.1847790599493386 6.304531381930762E-4 60-61 0.0 0.0 4.503236701379116E-5 1.3335660005629046 6.304531381930762E-4 62-63 0.0 0.0 4.503236701379116E-5 1.4706670419363919 8.10582606248241E-4 64-65 0.0 0.0 4.503236701379116E-5 1.6154461018857305 8.55614973262032E-4 66-67 0.0 0.0 4.503236701379116E-5 1.7850830284266817 8.55614973262032E-4 68-69 0.0 0.0 4.503236701379116E-5 1.9749845201238392 8.55614973262032E-4 70-71 0.0 0.0 4.503236701379116E-5 2.1713256403039685 9.006473402758232E-4 72-73 0.0 0.0 4.503236701379116E-5 2.370909090909091 9.681958907965099E-4 74-75 0.0 0.0 4.503236701379116E-5 2.5657416267942583 0.001013228257810301 76-77 0.0 0.0 4.503236701379116E-5 2.780320855614973 0.001080776808330988 78-79 0.0 0.0 4.503236701379116E-5 3.016920911905432 0.0011708415423585703 80-81 0.0 0.0 4.503236701379116E-5 3.2718491415705038 0.0011933577258654657 82-83 0.0 0.0 4.503236701379116E-5 3.532676611314382 0.0013059386433999438 84-85 0.0 0.0 4.503236701379116E-5 3.796588798198705 0.0013059386433999438 86-87 0.0 0.0 4.503236701379116E-5 4.059172530256122 0.0013284548269068392 88-89 0.0 0.0 4.503236701379116E-5 4.351567689276667 0.0013509710104137348 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6050 0.0 42.08596 1 GTATCAA 9425 0.0 36.440533 1 TATCAAC 11525 0.0 29.756712 2 ATCAACG 11595 0.0 29.451517 3 TCAACGC 11830 0.0 28.863873 4 CAACGCA 11990 0.0 28.557915 5 AACGCAG 12390 0.0 27.635948 6 ACGACCG 70 0.008401071 27.137657 5 GTAAGGT 1320 0.0 26.26391 4 TAAGGTG 1385 0.0 25.717096 5 GTGTAAG 825 0.0 24.759523 1 ACGCAGA 13975 0.0 24.467033 7 CGCAGAG 13940 0.0 24.392197 8 GTACATG 8345 0.0 22.82684 1 TACATGG 8075 0.0 22.823269 2 GGTAAGG 1475 0.0 22.218084 3 GAGTACT 8925 0.0 22.190525 12-13 ACATGGG 8085 0.0 21.5006 3 GCAGAGT 15495 0.0 21.423294 9 GTACTTT 9325 0.0 21.034924 14-15 >>END_MODULE