FastQCFastQC Report
Thu 26 May 2016
SRR938227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439660
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104280.42743661001942895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84710.34722051433396456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50430.20670913160030496No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28840.11821319364173695No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27500.11272062500512366No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27010.11071214841412327No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24860.1018994450046318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.055.9529531
GTATCAA86250.044.4516751
TATCAAC110750.034.5051962
ATCAACG110700.034.2202263
TCAACGC112350.033.717664
CAACGCA113400.033.3216325
AACGCAG116300.032.4840746
ACGCAGA131950.028.2990287
CGCAGAG133000.028.0398968
TGGTATC19450.027.125362
GCAGAGT144750.025.4329629
GCGATAC950.001226616325.0011659
GTGGTAT22600.024.6870171
GTGTAGC14900.024.3230761
GAGTACT97000.023.4798412-13
TAAGGTG15050.023.054655
TAGCCCT16650.022.266534
CAGAGTA138900.022.2290310-11
GTACTTT103650.021.74785414-15
AGAGTAC132600.021.5477310-11
Produced by FastQC (version 0.11.5)