FastQCFastQC Report
Thu 26 May 2016
SRR938227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439660
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96510.3955879097907085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82920.33988342637908564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.21158686046416306No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30480.1249354418238607No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27980.11468811227794036No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27740.11370436864153201No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27470.11259765705057263No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25700.10534254773206103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.053.5734251
GTATCAA81750.043.808551
ATCAACG105650.033.6246833
TATCAAC106900.033.501862
TCAACGC107700.032.895674
CAACGCA108250.032.6839335
AACGCAG111800.031.6915196
TGGTATC20700.028.2609312
ACGCAGA127300.027.6089157
CGCAGAG129350.026.9877668
GTGGTAT23100.026.9718671
GCAGAGT142900.023.996679
GAGTACT95950.022.34903712-13
CAGAGTA135900.021.63302210-11
TACATGG72250.021.5917422
GTACATG76400.021.4771881
AGAGTAC129750.021.23081210-11
GTAAGGT14400.020.8054084
GTACTTT102650.020.72836714-15
AGTACTT100800.020.33135212-13
Produced by FastQC (version 0.11.5)