FastQCFastQC Report
Thu 26 May 2016
SRR938226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420978
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101280.4183433306704976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83320.34415843514480515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.2092542765774823No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29200.12061241366092545No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26470.10933597909605128No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA25810.10660980810234541No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24660.10185966167391856No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24250.10016613120813159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.056.7345851
GTATCAA84300.044.0489121
ATCAACG107450.034.457083
TATCAAC109800.033.848072
TCAACGC109450.033.827444
CAACGCA110650.033.3317225
AACGCAG114400.032.3188636
TGGTATC19550.031.3597372
GTGGTAT22750.028.5325581
ACGCAGA129900.028.1941667
CGCAGAG129750.028.0803178
GCAGAGT143700.024.5599869
GAGTACT95650.023.5169212-13
GTGTAGC16250.023.2036841
GTTACGT1252.2479905E-422.8133283
GTACTTT100750.022.28301814-15
CAGAGTA136750.021.98710310-11
GTACATG74450.021.6688181
TACATGG72650.021.3915182
AGAGTAC131100.021.1593210-11
Produced by FastQC (version 0.11.5)