##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2420978 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.902294857698006 33.0 31.0 34.0 30.0 34.0 2 32.094657613576004 34.0 31.0 34.0 30.0 34.0 3 32.17980419483366 34.0 31.0 34.0 30.0 34.0 4 35.68747010505672 37.0 35.0 37.0 35.0 37.0 5 35.696522231924455 37.0 35.0 37.0 35.0 37.0 6 35.719272128866926 37.0 36.0 37.0 35.0 37.0 7 35.66874957145418 37.0 35.0 37.0 33.0 37.0 8 35.636693518074104 37.0 35.0 37.0 33.0 37.0 9 37.282923677951636 39.0 37.0 39.0 34.0 39.0 10-11 37.19963027338538 39.0 37.0 39.0 34.0 39.0 12-13 37.09818800501285 39.0 37.0 39.0 33.5 39.0 14-15 38.29891886667289 40.0 38.0 41.0 33.5 41.0 16-17 38.14980226999172 40.0 38.0 41.0 33.0 41.0 18-19 38.30044097881104 40.0 38.0 41.0 33.5 41.0 20-21 38.27628545158196 40.0 38.0 41.0 34.0 41.0 22-23 38.1905688527529 40.0 38.0 41.0 33.5 41.0 24-25 38.09048781112426 40.0 38.0 41.0 33.0 41.0 26-27 37.912599164469896 40.0 38.0 41.0 33.0 41.0 28-29 37.705910793076185 40.0 38.0 41.0 32.5 41.0 30-31 37.490444357610855 40.0 37.0 41.0 32.0 41.0 32-33 37.228217274175975 40.0 37.0 41.0 31.5 41.0 34-35 36.9949625729767 40.0 36.0 41.0 31.0 41.0 36-37 36.73201677173439 39.5 36.0 41.0 30.0 41.0 38-39 36.43855417108293 39.0 35.5 41.0 30.0 41.0 40-41 36.217110399185785 39.0 35.0 41.0 30.0 41.0 42-43 36.03937726819492 39.0 35.0 41.0 29.0 41.0 44-45 35.770744095981044 39.0 35.0 40.5 27.5 41.0 46-47 35.394459800956476 39.0 35.0 40.0 26.5 41.0 48-49 34.998270946700046 38.0 34.5 40.0 24.5 41.0 50-51 34.29889470288454 37.5 33.5 39.5 24.0 40.5 52-53 34.41671754142334 38.0 34.0 40.0 24.0 40.5 54-55 34.51822218128376 38.0 34.0 40.0 23.5 41.0 56-57 34.222571002297414 38.0 34.0 40.0 23.0 41.0 58-59 33.89909429164577 38.0 33.5 40.0 22.0 41.0 60-61 33.47710594643982 37.0 33.0 40.0 19.5 41.0 62-63 32.93397957354425 36.5 32.5 39.5 17.5 41.0 64-65 32.41406840541302 36.0 31.5 39.0 12.0 41.0 66-67 31.883714350151052 35.5 31.0 39.0 8.0 40.0 68-69 31.2735349102718 35.0 30.5 38.5 4.5 40.0 70-71 30.717780582888402 35.0 30.0 38.0 2.0 40.0 72-73 30.066169746276092 34.5 29.0 37.0 2.0 39.0 74-75 29.374807825597756 34.0 28.5 36.5 2.0 39.0 76-77 28.70683190842709 34.0 27.5 36.0 2.0 38.5 78-79 27.973726527048157 33.5 26.0 35.0 2.0 37.5 80-81 27.31778830703955 33.0 25.5 35.0 2.0 37.0 82-83 26.572056003813337 32.5 24.5 35.0 2.0 36.0 84-85 25.906325047150368 32.0 22.0 35.0 2.0 36.0 86-87 25.209059520573916 31.5 19.0 34.5 2.0 35.5 88-89 24.385293050990136 31.0 14.0 34.0 2.0 35.0 90-91 23.54896099840643 31.0 4.5 34.0 2.0 35.0 92-93 22.652127569932482 30.0 2.0 34.0 2.0 35.0 94-95 21.699323785676697 29.0 2.0 34.0 2.0 35.0 96-97 20.49353236584554 29.0 2.0 34.0 2.0 35.0 98-99 18.98625410887666 26.0 2.0 33.5 2.0 35.0 100-101 15.298587802119638 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4033346508554807 1101 2 0.33564676778599534 1101 3 0.2690266756848274 1101 4 0.2317243330733234 1101 5 0.279643440531558 1101 6 0.28002534748159036 1101 7 0.29341094818165203 1101 8 0.260600704266821 1101 9 0.4010277671819438 1101 10-11 0.35098276395493855 1101 12-13 0.38230789502369333 1101 14-15 0.5130370993384403 1101 16-17 0.6103992791001431 1101 18-19 0.5455603824381541 1101 20-21 0.5534727999371611 1101 22-23 0.5584906667255964 1101 24-25 0.5517885896714603 1101 26-27 0.6360837810402842 1101 28-29 0.5986747847494556 1101 30-31 0.6433630764479616 1101 32-33 0.5851174943834323 1101 34-35 0.6868597062027177 1101 36-37 0.6712385513252457 1101 38-39 0.7842800802421621 1101 40-41 0.7729073957751993 1101 42-43 0.8612508317766512 1101 44-45 0.8286524141703495 1101 46-47 1.0406062675346348 1101 48-49 1.091937283266624 1101 50-51 1.036807040721051 1101 52-53 1.1460699512957078 1101 54-55 1.1720161544799694 1101 56-57 1.2196340813462498 1101 58-59 1.338477433489011 1101 60-61 1.4091625826970144 1101 62-63 1.3425720306420246 1101 64-65 1.3172773290235398 1101 66-67 1.3599692890530193 1101 68-69 1.5361440642731878 1101 70-71 1.8851491916732535 1101 72-73 2.087896861237301 1101 74-75 2.1447295834102214 1101 76-77 2.2527514819314085 1101 78-79 2.452966875091157 1101 80-81 2.7661283630890807 1101 82-83 2.950720921209637 1101 84-85 2.8962167730397432 1101 86-87 3.027988905736766 1101 88-89 3.4397782129546854 1101 90-91 3.825314004287751 1101 92-93 3.8949436532414374 1101 94-95 4.260516437872067 1101 96-97 4.424538273429668 1101 98-99 4.816118004200636 1101 100-101 4.463196099328478 1102 1 0.3484825967250593 1102 2 0.2121876176696702 1102 3 0.1296776301032807 1102 4 0.06863674419653876 1102 5 0.13006746875648645 1102 6 0.1920667037628334 1102 7 0.059530584760636884 1102 8 0.2303561560141958 1102 9 0.31665588329657623 1102 10-11 0.3035175090832567 1102 12-13 0.3426285284301329 1102 14-15 0.4271383064206873 1102 16-17 0.42982060310857406 1102 18-19 0.34423422166423023 1102 20-21 0.4027112899382246 1102 22-23 0.3874404339802524 1102 24-25 0.3829344268200927 1102 26-27 0.4213480645998757 1102 28-29 0.4123612232127911 1102 30-31 0.47881936308256456 1102 32-33 0.45455509472154887 1102 34-35 0.5956971359171703 1102 36-37 0.6772291048809578 1102 38-39 0.5905967982756337 1102 40-41 0.5217343065772084 1102 42-43 0.7004245415740158 1102 44-45 0.7303245549789139 1102 46-47 0.742581423805909 1102 48-49 1.0575355834498836 1102 50-51 1.131269425937937 1102 52-53 1.0271560331201712 1102 54-55 1.0261989654412034 1102 56-57 1.1104337985373078 1102 58-59 0.9790852710664595 1102 60-61 1.1581277702822277 1102 62-63 1.2333713362950434 1102 64-65 1.1770706381556977 1102 66-67 1.0649393105641138 1102 68-69 1.2192543165501277 1102 70-71 1.407157320372722 1102 72-73 1.5794369765255674 1102 74-75 1.741627553663431 1102 76-77 1.892813208522817 1102 78-79 2.0939599122789687 1102 80-81 2.187042068087912 1102 82-83 2.4646642417208184 1102 84-85 2.5487141505135504 1102 86-87 2.539609299751522 1102 88-89 3.037868306040476 1102 90-91 3.1461203595874743 1102 92-93 3.503368598373129 1102 94-95 3.810364395141246 1102 96-97 4.131985548204156 1102 98-99 3.955933219502935 1102 100-101 3.7304387203484453 1103 1 0.49451743468044995 1103 2 0.5059114572421421 1103 3 0.5033966291732455 1103 4 0.44038440958118485 1103 5 0.3673335802617572 1103 6 0.4340771908676757 1103 7 0.40810922389629667 1103 8 0.5408695127758207 1103 9 0.5615784791721978 1103 10-11 0.5907354595403191 1103 12-13 0.5915428310415649 1103 14-15 0.8855692711933401 1103 16-17 0.7939322781859346 1103 18-19 0.8571501169571363 1103 20-21 0.7446393616323164 1103 22-23 0.8088854703669739 1103 24-25 0.8774681234513579 1103 26-27 0.9451816840368323 1103 28-29 0.9721341099577288 1103 30-31 0.9534247791754709 1103 32-33 0.9324668863222954 1103 34-35 0.9745520935774081 1103 36-37 1.0069524740658906 1103 38-39 0.9581292740462786 1103 40-41 1.0178487298108365 1103 42-43 1.0964615753218823 1103 44-45 1.0360230268049477 1103 46-47 1.2249026106659002 1103 48-49 1.1790563502791684 1103 50-51 1.246622699669338 1103 52-53 1.2732345917434387 1103 54-55 1.3993372993909858 1103 56-57 1.408019305135909 1103 58-59 1.417701160601517 1103 60-61 1.5146639613337385 1103 62-63 1.5100231333803933 1103 64-65 1.729567644558628 1103 66-67 1.8810431549575668 1103 68-69 1.8837006304001136 1103 70-71 2.199066215479231 1103 72-73 2.352529881968888 1103 74-75 2.3486875632078075 1103 76-77 2.492654916283332 1103 78-79 2.8197464207444796 1103 80-81 2.904665596053036 1103 82-83 3.165316733652503 1103 84-85 3.2495215678029297 1103 86-87 3.2206811782861386 1103 88-89 3.581341337317344 1103 90-91 3.9125775801826457 1103 92-93 4.203972538893506 1103 94-95 4.4564646189169 1103 96-97 4.635087715012169 1103 98-99 4.483113714619979 1103 100-101 4.44555318944974 1104 1 0.294212484138054 1104 2 0.24693131245816602 1104 3 0.21032507715785442 1104 4 0.16688977909294778 1104 5 0.23138793912394107 1104 6 0.19166896273792844 1104 7 0.12251575701998263 1104 8 0.17558220083492415 1104 9 0.2905989047037352 1104 10-11 0.28256039835759594 1104 12-13 0.2905827261176839 1104 14-15 0.4834410378740799 1104 16-17 0.5291164728300188 1104 18-19 0.34891751501212553 1104 20-21 0.38046921277907586 1104 22-23 0.3990510035610626 1104 24-25 0.4347941318316302 1104 26-27 0.43195738221947977 1104 28-29 0.4964179056908975 1104 30-31 0.5517378983364409 1104 32-33 0.520095002523199 1104 34-35 0.5435228556083871 1104 36-37 0.6411489778723478 1104 38-39 0.7338217004067289 1104 40-41 0.7070186904776605 1104 42-43 0.7343514034700505 1104 44-45 0.842121949094583 1104 46-47 0.852554766020198 1104 48-49 1.0530021189409453 1104 50-51 0.9377472529480073 1104 52-53 0.93684252816211 1104 54-55 1.122601656132197 1104 56-57 1.2313236680939141 1104 58-59 1.306082376675377 1104 60-61 1.3246325497009863 1104 62-63 1.3392188379481027 1104 64-65 1.5338577633551722 1104 66-67 1.5993579877218451 1104 68-69 1.6548328572563946 1104 70-71 1.8317945331945715 1104 72-73 1.869941110011407 1104 74-75 1.9956247297580916 1104 76-77 1.9352643710342434 1104 78-79 2.1918926845357802 1104 80-81 2.3114628449005608 1104 82-83 2.483909462395296 1104 84-85 2.649857380916554 1104 86-87 2.727795067373055 1104 88-89 2.992181605135386 1104 90-91 3.235067317381432 1104 92-93 3.337597952294768 1104 94-95 3.650812251908235 1104 96-97 3.9211570721098283 1104 98-99 4.10503603658346 1104 100-101 4.074191109498415 1105 1 0.2202566449973098 1105 2 0.14960252991669876 1105 3 0.1279828887848211 1105 4 0.06449863699113223 1105 5 0.13590806930990595 1105 6 0.148469658688839 1105 7 0.17331856601401086 1105 8 0.15011932781984427 1105 9 0.2494985746831162 1105 10-11 0.104414751132623 1105 12-13 0.18574590744679398 1105 14-15 0.1713348048658503 1105 16-17 0.25321829551393904 1105 18-19 0.20907094177314178 1105 20-21 0.19880227366339653 1105 22-23 0.06395839211042897 1105 24-25 0.16130980814612172 1105 26-27 0.16419607904670386 1105 28-29 0.22449558581877938 1105 30-31 0.20331038697062098 1105 32-33 0.24998918622798527 1105 34-35 0.29557694254463485 1105 36-37 0.37386284141827275 1105 38-39 0.3823900199009884 1105 40-41 0.46044509976486836 1105 42-43 0.47375796596293185 1105 44-45 0.5486513126224182 1105 46-47 0.49289593471929294 1105 48-49 0.5940127585217212 1105 50-51 0.6627784729255737 1105 52-53 0.5938172118421008 1105 54-55 0.8178904878216784 1105 56-57 0.8272421919919353 1105 58-59 0.9410514146310973 1105 60-61 0.9192674367530032 1105 62-63 0.903799890637508 1105 64-65 1.0641722945798406 1105 66-67 1.178978309917369 1105 68-69 1.2803253591021289 1105 70-71 1.3542225865552773 1105 72-73 1.555986717976925 1105 74-75 1.5557639701880994 1105 76-77 1.6387409935686321 1105 78-79 1.7795482987050946 1105 80-81 1.9621130545299827 1105 82-83 2.187965513644297 1105 84-85 2.2520842161621673 1105 86-87 2.384266194923409 1105 88-89 2.541006032288994 1105 90-91 2.7069374308854783 1105 92-93 2.8637837084814812 1105 94-95 3.152419561536714 1105 96-97 3.348745399188001 1105 98-99 3.6281956825515955 1105 100-101 3.7809101544886765 1106 1 0.18180318280527175 1106 2 0.16549497065806662 1106 3 0.12016896094898044 1106 4 0.12215031406837795 1106 5 0.18042284653996887 1106 6 0.02383777704667267 1106 7 0.15978243921538393 1106 8 0.16469451405014013 1106 9 0.13898286549142824 1106 10-11 0.15319934486087305 1106 12-13 0.2012982599693771 1106 14-15 0.2822042932979869 1106 16-17 0.17863997898141548 1106 18-19 0.28380042662779914 1106 20-21 0.18395877581312448 1106 22-23 0.23339288648561052 1106 24-25 0.19310603130361415 1106 26-27 0.21571409740336378 1106 28-29 0.29022642389725206 1106 30-31 0.2430627949702071 1106 32-33 0.32448576169030474 1106 34-35 0.3112216487045245 1106 36-37 0.43098121674604783 1106 38-39 0.20485983808422503 1106 40-41 0.5520254778898206 1106 42-43 0.6015788835109035 1106 44-45 0.7840855237797726 1106 46-47 0.7544582860623379 1106 48-49 0.8074902164570616 1106 50-51 0.8383121149252872 1106 52-53 0.8516048918020758 1106 54-55 0.5255719448963632 1106 56-57 0.9332293324770475 1106 58-59 1.040967923702489 1106 60-61 1.1065141123461046 1106 62-63 0.8763315695553402 1106 64-65 1.084388667810373 1106 66-67 1.1308008198364803 1106 68-69 0.9179953870091282 1106 70-71 1.1599175817010376 1106 72-73 1.3107544339969444 1106 74-75 1.3890121059408145 1106 76-77 1.6339840521396312 1106 78-79 1.808584120250643 1106 80-81 2.1254659808764984 1106 82-83 2.161462288071995 1106 84-85 2.3366407761220387 1106 86-87 2.2078831424963568 1106 88-89 2.354606579800244 1106 90-91 2.5662788716799874 1106 92-93 2.688813261645418 1106 94-95 3.1255547795755554 1106 96-97 3.353626126895776 1106 98-99 3.6836777782597743 1106 100-101 3.637096989461476 1107 1 0.298260395085304 1107 2 0.20676625389486247 1107 3 0.04582220455374397 1107 4 0.13077376290065956 1107 5 0.12798776827894898 1107 6 0.19939998128599967 1107 7 0.09688076563900694 1107 8 0.1619681020018291 1107 9 0.1742384363266254 1107 10-11 0.1844789697896303 1107 12-13 0.18084391529254162 1107 14-15 0.2852681256135625 1107 16-17 0.3319522248510225 1107 18-19 0.2853213977352098 1107 20-21 0.2880081317359995 1107 22-23 0.31196935759810884 1107 24-25 0.32187884880193707 1107 26-27 0.4346704555785763 1107 28-29 0.45117784585473686 1107 30-31 0.5254411856881731 1107 32-33 0.4641530477947029 1107 34-35 0.4666523090741421 1107 36-37 0.30857779313429035 1107 38-39 0.4680732110594832 1107 40-41 0.5732951506406394 1107 42-43 0.7988780359731109 1107 44-45 0.6890125945637564 1107 46-47 0.7480798234197579 1107 48-49 0.7640484515520569 1107 50-51 0.5235569361574335 1107 52-53 0.6115128595642148 1107 54-55 0.6517057543915072 1107 56-57 0.7610392200791978 1107 58-59 0.7809612941291206 1107 60-61 0.8768487259897384 1107 62-63 0.9935632223283051 1107 64-65 0.9863384503556745 1107 66-67 1.1726140394246798 1107 68-69 1.3313498338658292 1107 70-71 1.344360287098585 1107 72-73 1.4951559390977245 1107 74-75 1.4994600946705425 1107 76-77 1.6943166096773723 1107 78-79 1.8869523226902984 1107 80-81 2.109374802352562 1107 82-83 2.2120262317925494 1107 84-85 2.171128551673455 1107 86-87 2.280579541652962 1107 88-89 2.606610813971159 1107 90-91 2.796891786683073 1107 92-93 3.084585657759302 1107 94-95 3.3933115993191727 1107 96-97 3.396510945094583 1107 98-99 3.5235009531530785 1107 100-101 3.5049419950395446 1108 1 0.16576286688271935 1108 2 0.09922676349552262 1108 3 0.14982113224925087 1108 4 0.13512081304671142 1108 5 0.17645894369486115 1108 6 0.17271375610657458 1108 7 0.19034806675249882 1108 8 0.15096658917897798 1108 9 0.20152647734528273 1108 10-11 0.23477080378424375 1108 12-13 0.267159156248546 1108 14-15 0.3751105254671714 1108 16-17 0.42836745970755175 1108 18-19 0.2968754034024599 1108 20-21 0.2143983436441559 1108 22-23 0.14521019462815588 1108 24-25 0.15657113732113004 1108 26-27 0.17153310647493214 1108 28-29 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2135.0 5 2296.0 6 2673.0 7 3434.0 8 4094.0 9 5469.0 10 6822.0 11 8432.0 12 10578.0 13 13424.0 14 15717.0 15 19143.0 16 22805.0 17 27864.0 18 27317.0 19 23507.0 20 23296.0 21 24694.0 22 26829.0 23 29784.0 24 33880.0 25 37621.0 26 42911.0 27 48735.0 28 56540.0 29 65884.0 30 77290.0 31 94158.0 32 114202.0 33 145069.0 34 188450.0 35 256098.0 36 339256.0 37 386562.0 38 211913.0 39 13204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45164217788528 18.51657282378078 14.827798408135845 23.2039865901981 2 15.316196509410377 19.423248971563286 40.92138838439875 24.339166134627582 3 19.370764858846538 23.422572651423604 29.67661251527089 27.53004997445897 4 12.750346966492005 16.539080536729678 37.147154296143654 33.563418200634665 5 13.741837732362372 36.99144861001156 34.47878275500692 14.78793090261916 6 32.448090273543286 36.05290540269595 17.097974789981972 14.401029533778786 7 28.617234717386587 32.88609713512341 20.170218920789726 18.326449226700277 8 26.285045811775237 35.05694808825249 19.434457116104017 19.223548983868255 9 26.019422049592798 17.079154832142958 20.245579539363764 36.65584357890047 10-11 25.00610087846451 26.521433042109805 26.986256957636034 21.486209121789653 12-13 25.95399090231926 23.82821644752407 27.465511772952635 22.75228087720404 14-15 22.580017326507136 25.347093161277634 26.01930626615837 26.053583246056856 16-17 22.017918337307883 28.484420732576606 26.91010955053742 22.587551379578088 18-19 22.00860365515869 26.807624064199082 29.475959749324886 21.70781253131734 20-21 22.39982542305668 26.00921612513993 29.967781378748686 21.623177073054705 22-23 22.246976579650347 25.893016543680076 29.761060527916634 22.098946348752943 24-25 22.04217897565345 26.02172808725624 29.880728639882413 22.055364297207895 26-27 21.646775300788285 26.45033661092786 29.6135163474005 22.28937174088336 28-29 21.829455288478723 26.860819959001432 29.407268784864126 21.90245596765572 30-31 21.93162404465166 26.390570617878023 29.326724792730634 22.351080544739684 32-33 21.561109926457824 26.40207848796365 29.417799388057702 22.619012197520824 34-35 22.35725016066008 26.566234313686998 29.176676495688525 21.899839029964397 36-37 22.416378046729363 26.108591899742507 29.171055714633432 22.3039743388947 38-39 21.919668901643924 26.534122929808934 29.090078618093234 22.45612955045391 40-41 22.528236084717445 26.354271270102707 29.050057398687752 22.06743524649209 42-43 22.100378271034263 26.392270223225566 28.979246635152002 22.528104870588173 44-45 22.131599603404116 26.61604560852681 29.10706023300008 22.145294555068993 46-47 22.540044159304028 26.891727071702675 28.319198885440322 22.24902988355297 48-49 22.214025448606368 26.90694116378393 28.35492612781155 22.52410725979815 50-51 22.437379651727955 27.213571628936712 28.193626400956134 22.1554223183792 52-53 22.748650798552504 26.8515617108744 28.171359273336737 22.228428217236356 54-55 22.207345120349604 27.124569265211175 28.349403219816132 22.31868239462309 56-57 22.342720777132367 27.219235463728598 28.17210173629049 22.26594202284855 58-59 22.43310570476037 26.819396763625697 28.046050375731735 22.701447155882196 60-61 22.02753498679313 27.11098707901875 28.342499061668764 22.51897887251936 62-63 22.12864272199225 27.031785770352563 28.527832172454538 22.311739335200652 64-65 21.946706327074423 27.10334428125839 28.33031748982669 22.61963190184049 66-67 21.846231762344434 27.118860417879276 28.256401846206973 22.778505973569317 68-69 22.323471448468684 26.770025978564842 28.43827658651118 22.468225986455288 70-71 22.171576026478874 26.84283032131231 28.054235176227348 22.93135847598147 72-73 22.197560675838833 26.938822725023986 28.054272845296364 22.809343753840817 74-75 22.155021946811257 27.27702556158017 27.936483346243225 22.631469145365347 76-77 22.27657228592898 26.852780177194703 28.03922127526152 22.831426261614794 78-79 22.293854200705685 27.122371912424413 27.95541985161708 22.62835403525282 80-81 22.534588084818424 27.104111075169 27.659519970387002 22.70178086962557 82-83 22.470012325357207 27.12485378226524 27.77664973780472 22.628484154572828 84-85 22.47850736236832 27.170750720517944 27.7216554448394 22.629086472274338 86-87 22.604386067455273 27.200995665648996 27.453609060788725 22.741009206107 88-89 22.54889605807037 27.221023073782884 27.310580455790262 22.91950041235648 90-91 22.476310421183417 27.199103198676784 27.41284966223266 22.911736717907143 92-93 22.57714954395554 26.781454983748475 27.602076807162028 23.039318665133955 94-95 22.440508176477966 26.6811658069036 27.657678150076638 23.2206478665418 96-97 22.670207579288345 26.381059216289028 27.63876493628836 23.309968268134266 98-99 22.44700315841902 26.54908481457174 27.349214878056188 23.65469714895306 100-101 23.300864240609815 25.504250670524375 27.48592503819822 23.70896005066759 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 661.0 1 456.0 2 372.5 3 498.5 4 401.0 5 371.0 6 743.5 7 1628.0 8 2777.5 9 3319.5 10 2794.0 11 2121.5 12 2043.0 13 2246.0 14 2450.0 15 2636.0 16 2844.0 17 3104.5 18 3392.5 19 3824.5 20 4376.5 21 5103.5 22 6266.0 23 7719.0 24 9632.5 25 12345.5 26 15852.0 27 19733.0 28 23050.5 29 26278.0 30 29834.5 31 35051.5 32 42125.5 33 45123.0 34 45922.5 35 49356.0 36 53592.5 37 57745.5 38 61121.0 39 63867.5 40 67972.5 41 73056.0 42 76181.5 43 78951.0 44 82040.5 45 85323.5 46 87600.0 47 89400.5 48 89855.0 49 87637.0 50 86369.5 51 85105.5 52 83049.0 53 81952.5 54 82344.5 55 78440.0 56 71728.0 57 66190.5 58 61616.5 59 54724.0 60 44091.5 61 36216.5 62 30490.5 63 23325.5 64 16803.0 65 12647.0 66 9373.5 67 6912.5 68 5207.0 69 3934.0 70 2964.0 71 2057.0 72 1411.5 73 1056.0 74 760.0 75 563.5 76 392.5 77 256.5 78 171.0 79 109.0 80 71.0 81 46.5 82 32.0 83 21.0 84 13.5 85 12.0 86 10.5 87 8.5 88 8.5 89 7.0 90 4.0 91 5.0 92 3.0 93 0.5 94 1.0 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45845108877486707 2 0.04308176282477578 3 0.05543214353868561 4 0.05931487192366061 5 0.08562655257503372 6 0.08339604903472894 7 0.047625381147618856 8 0.03994253561990237 9 0.04754276990538534 10-11 0.05140484547980197 12-13 0.04669600467249186 14-15 0.0407479952316791 16-17 0.04126431549563854 18-19 0.049256953181730685 20-21 0.05842680106965037 22-23 0.04964935658233986 24-25 0.06695641183026034 26-27 0.058612676364675764 28-29 0.04698514402030914 30-31 0.04874063291777125 32-33 0.047171019315334545 34-35 0.052582055681629485 36-37 0.046634046240816726 38-39 0.02767476614822605 40-41 0.025877971629647188 42-43 0.025754054766296927 44-45 0.015613524782133502 46-47 0.02684865372589094 48-49 0.026807348104774188 50-51 0.016480942825585362 52-53 0.015675483213808634 54-55 0.016728776552285895 56-57 0.01596462256162592 58-59 0.015200468570965948 60-61 0.018897321660915546 62-63 0.01643963720446861 64-65 0.017678805837971268 66-67 0.009541598477970472 68-69 0.013568896536854115 70-71 0.01992996218883443 72-73 0.017782069890763152 74-75 0.01458088425421462 76-77 0.010367710900305579 78-79 0.014415661769747599 80-81 0.017307055247920467 82-83 0.014808065170356774 84-85 0.013362368431270338 86-87 0.0035109777949242 88-89 0.03064877086863243 90-91 0.033911914936856094 92-93 0.01798859799634693 94-95 0.03446954082193229 96-97 0.028831323539495197 98-99 0.025671443524063416 100-101 0.15091008675006545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2420978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.91475532400082 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39521289055597 43.48014849590947 2 13.692559503489003 15.586173478007973 3 4.371909619384028 7.464784988576626 4 1.9801778790592302 4.508053579386199 5 1.0348162123426703 2.944815576539618 6 0.5993556581617285 2.046730838179815 7 0.3785059296959725 1.5079800664130814 8 0.27308592584150965 1.2434094921358203 9 0.19790552701111813 1.01373701823947 >10 0.9746166609618946 9.959778053893208 >50 0.0635640455116328 2.511116237538275 >100 0.03297242090680358 3.525011274941386 >500 0.0026952863273624504 1.0649799781727385 >1k 0.0024039040216997623 2.1678957479908663 >5k 1.4569115283028864E-4 0.5554794934227395 >10k+ 7.284557641514432E-5 0.41990568065274714 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10128 0.4183433306704976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8332 0.34415843514480515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5066 0.2092542765774823 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2920 0.12061241366092545 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2647 0.10933597909605128 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2581 0.10660980810234541 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2466 0.10185966167391856 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2425 0.10016613120813159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.1305621116755296E-5 2 0.0 0.0 0.0 0.0 4.1305621116755296E-5 3 0.0 0.0 0.0 0.0 4.1305621116755296E-5 4 0.0 0.0 0.0 0.0 4.1305621116755296E-5 5 4.1305621116755296E-5 0.0 0.0 0.0 4.1305621116755296E-5 6 4.1305621116755296E-5 0.0 0.0 0.0 4.1305621116755296E-5 7 4.1305621116755296E-5 0.0 0.0 0.0 4.1305621116755296E-5 8 4.1305621116755296E-5 0.0 0.0 0.0 4.1305621116755296E-5 9 4.1305621116755296E-5 0.0 0.0 0.0 4.1305621116755296E-5 10-11 4.1305621116755296E-5 0.0 0.0 0.0 8.261124223351059E-5 12-13 8.261124223351059E-5 0.0 0.0 2.0652810558377648E-5 1.4456967390864353E-4 14-15 1.6522248446702119E-4 0.0 0.0 8.261124223351059E-5 1.858752950253988E-4 16-17 1.858752950253988E-4 0.0 0.0 1.2391686335026588E-4 2.271809161421541E-4 18-19 2.0652810558377646E-4 0.0 0.0 1.2391686335026588E-4 2.4783372670053176E-4 20-21 2.0652810558377646E-4 0.0 0.0 1.4456967390864353E-4 2.4783372670053176E-4 22-23 2.4783372670053176E-4 0.0 0.0 3.3044496893404237E-4 2.8913934781728707E-4 24-25 2.8913934781728707E-4 0.0 0.0 6.195843167513294E-4 3.3044496893404237E-4 26-27 2.8913934781728707E-4 0.0 0.0 0.001280474254619414 3.3044496893404237E-4 28-29 4.3370902172593063E-4 0.0 0.0 0.004006645248325264 3.3044496893404237E-4 30-31 4.956674534010635E-4 0.0 0.0 0.01212319979776768 3.3044496893404237E-4 32-33 4.956674534010635E-4 0.0 0.0 0.028955240402845465 3.717505900507977E-4 34-35 4.956674534010635E-4 0.0 0.0 0.05380057150457377 3.717505900507977E-4 36-37 4.956674534010635E-4 0.0 0.0 0.0965518893604155 3.717505900507977E-4 38-39 5.369730745178188E-4 0.0 0.0 0.15941904470011706 3.924034006091753E-4 40-41 5.369730745178188E-4 0.0 0.0 0.22253403376651915 4.130562111675529E-4 42-43 5.989315061929517E-4 0.0 0.0 0.28759038702540873 4.130562111675529E-4 44-45 6.815427484264623E-4 0.0 0.0 0.3686113628459243 4.337090217259306E-4 46-47 8.054596117767282E-4 0.0 0.0 0.4635523329827863 4.543618322843082E-4 48-49 8.261124223351058E-4 0.0 0.0 0.5618803640512222 4.956674534010635E-4 50-51 8.261124223351058E-4 0.0 0.0 0.6665488079610802 4.956674534010635E-4 52-53 8.261124223351058E-4 0.0 0.0 0.7606430128650488 4.956674534010635E-4 54-55 8.261124223351058E-4 0.0 0.0 0.8646505668370386 5.163202639594411E-4 56-57 9.087236645686165E-4 0.0 0.0 0.9893935426096396 5.369730745178188E-4 58-59 9.087236645686165E-4 0.0 0.0 1.120724764950363 5.576258850761965E-4 60-61 9.087236645686165E-4 0.0 0.0 1.256186549402762 5.782786956345741E-4 62-63 9.087236645686165E-4 0.0 0.0 1.3897069696626736 6.195843167513294E-4 64-65 9.087236645686165E-4 0.0 0.0 1.5266970620963924 6.195843167513294E-4 66-67 9.500292856853718E-4 0.0 0.0 1.688718360926865 6.402371273097071E-4 68-69 9.500292856853718E-4 0.0 0.0 1.8611280234682017 6.608899378680847E-4 70-71 0.0010119877173605048 0.0 0.0 2.040311807872686 6.815427484264623E-4 72-73 0.0010739461490356377 0.0 0.0 2.2268892984570696 7.848068012183507E-4 74-75 0.0010739461490356377 0.0 0.0 2.4155114172867327 8.674180434518611E-4 76-77 0.0010739461490356377 0.0 0.0 2.6197470608985296 8.674180434518611E-4 78-79 0.0010739461490356377 0.0 0.0 2.842425664338957 8.674180434518611E-4 80-81 0.0011978630123859035 0.0 0.0 3.073799101024462 8.674180434518611E-4 82-83 0.001280474254619414 0.0 0.0 3.3146728305668205 9.087236645686165E-4 84-85 0.001342432686294547 0.0 0.0 3.556290061289281 9.087236645686165E-4 86-87 0.00140439111796968 0.0 0.0 3.8053836094338735 0.0010326405279188823 88-89 0.00140439111796968 0.0 0.0 4.0732712151865895 0.0011152517701523929 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 56.734585 1 GTATCAA 8430 0.0 44.048912 1 ATCAACG 10745 0.0 34.45708 3 TATCAAC 10980 0.0 33.84807 2 TCAACGC 10945 0.0 33.82744 4 CAACGCA 11065 0.0 33.331722 5 AACGCAG 11440 0.0 32.318863 6 TGGTATC 1955 0.0 31.359737 2 GTGGTAT 2275 0.0 28.532558 1 ACGCAGA 12990 0.0 28.194166 7 CGCAGAG 12975 0.0 28.080317 8 GCAGAGT 14370 0.0 24.559986 9 GAGTACT 9565 0.0 23.51692 12-13 GTGTAGC 1625 0.0 23.203684 1 GTTACGT 125 2.2479905E-4 22.813328 3 GTACTTT 10075 0.0 22.283018 14-15 CAGAGTA 13675 0.0 21.987103 10-11 GTACATG 7445 0.0 21.668818 1 TACATGG 7265 0.0 21.391518 2 AGAGTAC 13110 0.0 21.15932 10-11 >>END_MODULE