FastQCFastQC Report
Thu 26 May 2016
SRR938226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420978
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.41206487626075083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83080.343167100238003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53760.22205901912367648No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29950.12371033524468211No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28220.11656446279148344No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26690.11024470276061989No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26320.10871639477929994No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24600.10161182794721803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.052.9786951
GTATCAA83850.042.376181
ATCAACG106200.033.1428643
TATCAAC107850.032.814022
TCAACGC107850.032.8092734
CAACGCA109450.032.3296475
AACGCAG113250.031.3287376
TGGTATC20900.030.0020482
ACGCAGA127500.027.5665237
GTGGTAT23500.027.2890971
CGCAGAG128450.027.2886928
GCAGAGT142500.024.2648169
ACGACCG1000.001654907723.7481865
TAAGGTG17850.023.1494945
GAGTACT98950.022.22417612-13
GTAAGGT16750.022.1177164
CAGAGTA136600.021.43595510-11
AGAGTAC131850.021.19955810-11
GTACTTT103700.021.02298714-15
AGTACTT103000.020.3127712-13
Produced by FastQC (version 0.11.5)