##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938226_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2420978 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50432469853092 33.0 31.0 34.0 31.0 34.0 2 32.734598579582304 34.0 31.0 34.0 31.0 34.0 3 32.69856438183247 34.0 31.0 34.0 31.0 34.0 4 36.2201217854933 37.0 35.0 37.0 35.0 37.0 5 36.18292689979008 37.0 35.0 37.0 35.0 37.0 6 36.240947253547944 37.0 37.0 37.0 35.0 37.0 7 36.20552355287822 37.0 37.0 37.0 35.0 37.0 8 36.197290516477224 37.0 37.0 37.0 35.0 37.0 9 37.90825443271273 39.0 38.0 39.0 35.0 39.0 10-11 37.87006325542818 39.0 38.0 39.0 35.0 39.0 12-13 37.813836804795415 39.0 38.0 39.0 35.0 39.0 14-15 39.09987616574789 40.0 38.5 41.0 36.0 41.0 16-17 39.038485479835 40.0 38.0 41.0 36.0 41.0 18-19 39.03315498942989 40.0 38.5 41.0 36.0 41.0 20-21 39.03212255543008 40.0 38.5 41.0 35.5 41.0 22-23 39.06322238368131 40.0 39.0 41.0 35.5 41.0 24-25 39.01884259171294 40.0 38.5 41.0 35.0 41.0 26-27 38.85307074248506 40.0 38.0 41.0 35.0 41.0 28-29 38.77373978615253 40.0 38.0 41.0 35.0 41.0 30-31 38.55904783108314 40.0 38.0 41.0 34.5 41.0 32-33 38.35689894745016 40.0 38.0 41.0 34.0 41.0 34-35 38.15250675553433 40.0 38.0 41.0 34.0 41.0 36-37 38.06759912729484 40.0 38.0 41.0 34.0 41.0 38-39 38.016760168824334 40.0 38.0 41.0 33.0 41.0 40-41 37.89378176918584 40.0 38.0 41.0 33.0 41.0 42-43 37.70764273776961 40.0 37.0 41.0 33.0 41.0 44-45 37.46230077266295 40.0 37.0 41.0 33.0 41.0 46-47 37.11853308869391 40.0 37.0 41.0 31.5 41.0 48-49 36.745590005361464 40.0 36.0 41.0 31.0 41.0 50-51 36.44824942647145 39.5 36.0 41.0 30.0 41.0 52-53 35.98596848050664 39.0 35.0 40.5 29.0 41.0 54-55 35.67448093291223 39.0 35.0 40.0 28.0 41.0 56-57 35.40097762144059 38.0 35.0 40.0 28.0 41.0 58-59 35.02726232952138 38.0 34.5 40.0 27.0 41.0 60-61 34.61653699455344 38.0 34.0 40.0 26.0 41.0 62-63 34.186870760494315 37.0 34.0 40.0 26.0 41.0 64-65 33.75114685057031 36.5 33.0 39.0 24.5 41.0 66-67 33.245262864842225 36.0 32.5 39.0 23.0 40.0 68-69 32.71715315050364 35.0 32.0 39.0 22.0 40.0 70-71 32.1289778345776 35.0 31.0 38.0 20.5 40.0 72-73 31.657063591655934 35.0 31.0 37.0 18.5 39.5 74-75 31.104429697419803 34.5 30.5 36.5 17.5 39.0 76-77 29.90425315719515 33.5 29.5 35.0 16.0 37.5 78-79 30.58794131958242 34.0 30.0 36.0 16.0 38.0 80-81 30.40344583883042 34.0 30.0 35.5 11.5 37.0 82-83 29.938261107701102 34.0 30.0 35.0 7.0 37.0 84-85 29.42551192121531 34.0 29.0 35.0 3.5 36.5 86-87 28.88236076494706 34.0 29.0 35.0 2.0 36.0 88-89 28.26024255486832 33.5 28.0 35.0 2.0 35.5 90-91 27.73490775215636 33.0 27.0 35.0 2.0 35.0 92-93 27.10853072601238 33.0 25.5 35.0 2.0 35.0 94-95 26.37369587827729 32.0 25.0 34.5 2.0 35.0 96-97 25.562157524768914 32.0 22.5 34.0 2.0 35.0 98-99 24.547144790245927 32.0 10.0 34.0 2.0 35.0 100-101 21.065792212899083 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03201960435046658 1101 2 0.027648057115946756 1101 3 0.08435058451582478 1101 4 0.03764436327871579 1101 5 -0.013272709158712814 1101 6 -0.01571899175550584 1101 7 0.035645713290598735 1101 8 0.11342729630944604 1101 9 0.07663599854781467 1101 10-11 0.07767073123034152 1101 12-13 0.10765725835247508 1101 14-15 0.1631665352967957 1101 16-17 0.19458543479001378 1101 18-19 0.230783841482058 1101 20-21 0.2824177786272912 1101 22-23 0.2273150973057838 1101 24-25 0.18984688638600034 1101 26-27 0.20272201350613273 1101 28-29 0.16196400643634945 1101 30-31 0.17199515343251193 1101 32-33 0.25770110795786394 1101 34-35 0.2269778136288707 1101 36-37 0.15033474460734908 1101 38-39 0.2542164139554899 1101 40-41 0.3679707404351049 1101 42-43 0.37188495637990826 1101 44-45 0.5118127440756624 1101 46-47 0.506759725153195 1101 48-49 0.5392719052406605 1101 50-51 0.6514203660486189 1101 52-53 0.5940624383848956 1101 54-55 0.6337758750917857 1101 56-57 0.6164842121012839 1101 58-59 0.6420892800672462 1101 60-61 0.7023461036441958 1101 62-63 0.712624226614686 1101 64-65 0.6856108936057623 1101 66-67 0.7035546987174612 1101 68-69 0.834888258525659 1101 70-71 0.8868171566362761 1101 72-73 0.8911770540616715 1101 74-75 0.892820661179293 1101 76-77 0.934309635077053 1101 78-79 0.9270664291960209 1101 80-81 1.0952507669150826 1101 82-83 1.2303029225464748 1101 84-85 1.285342064510619 1101 86-87 1.4256388771797717 1101 88-89 1.4224242021990463 1101 90-91 1.5401240922183383 1101 92-93 1.9006277102497933 1101 94-95 1.8491411328179161 1101 96-97 1.9668022086882821 1101 98-99 2.3365018897920713 1101 100-101 2.6690185236849757 1102 1 0.05934657438297819 1102 2 0.06920167085210238 1102 3 0.18436009104467388 1102 4 0.05359989906215645 1102 5 0.06355774004740766 1102 6 0.05422724902964404 1102 7 0.06340951020645491 1102 8 0.0454508823778923 1102 9 -0.023543473193718967 1102 10-11 0.12554114530757943 1102 12-13 0.10275450273487508 1102 14-15 0.19768274557463172 1102 16-17 0.1763331051760204 1102 18-19 0.1821292416595952 1102 20-21 0.14144377507406602 1102 22-23 0.21670358880896856 1102 24-25 0.15146674540129368 1102 26-27 0.21599308662040073 1102 28-29 0.11941375043910085 1102 30-31 0.15306532931153072 1102 32-33 0.14078788198931846 1102 34-35 0.11423315940518819 1102 36-37 0.20349836102261776 1102 38-39 0.32703354414431374 1102 40-41 0.3052647913645856 1102 42-43 0.383995286437532 1102 44-45 0.37674297397316536 1102 46-47 0.36402176665351504 1102 48-49 0.4223868696282054 1102 50-51 0.4613446265867722 1102 52-53 0.4085398529712023 1102 54-55 0.36089724054210137 1102 56-57 0.4294497387938492 1102 58-59 0.3882226743630781 1102 60-61 0.3766523225190639 1102 62-63 0.4083487372856567 1102 64-65 0.43817847947008914 1102 66-67 0.5021093976794333 1102 68-69 0.7321439788016022 1102 70-71 0.7143104907487938 1102 72-73 0.7517119547513715 1102 74-75 0.7633959584435104 1102 76-77 0.8209624891551321 1102 78-79 0.9435767182644561 1102 80-81 0.9946084177571493 1102 82-83 1.100861655404369 1102 84-85 1.177601301379159 1102 86-87 1.1369243615332998 1102 88-89 1.1388602909579255 1102 90-91 1.295871838327347 1102 92-93 1.334651474102678 1102 94-95 1.2937328850193346 1102 96-97 1.5694088289362007 1102 98-99 1.8772147313017733 1102 100-101 1.9429730215694647 1103 1 0.2792366481323256 1103 2 0.2990494194816833 1103 3 0.3812226451709577 1103 4 0.18255293545278306 1103 5 0.16858054213233942 1103 6 0.1260130724079076 1103 7 0.19904849069512665 1103 8 0.18874293155974442 1103 9 0.1915607488760429 1103 10-11 0.3609078963252088 1103 12-13 0.2673881908757423 1103 14-15 0.45546073046172353 1103 16-17 0.4590813980693653 1103 18-19 0.4106468280054756 1103 20-21 0.4030824510795199 1103 22-23 0.4132858134297521 1103 24-25 0.4611913247118551 1103 26-27 0.487577482635956 1103 28-29 0.4755132227657768 1103 30-31 0.488629750446961 1103 32-33 0.48171418436263025 1103 34-35 0.5709989815538066 1103 36-37 0.6567514031710928 1103 38-39 0.6872208501216548 1103 40-41 0.5977189749347858 1103 42-43 0.7121596429678689 1103 44-45 0.7367050892337303 1103 46-47 0.793731033971234 1103 48-49 0.8425898194070172 1103 50-51 0.7697273327204499 1103 52-53 0.8274987643265774 1103 54-55 0.7839808260662693 1103 56-57 0.794242841468126 1103 58-59 0.8662115626772646 1103 60-61 0.9258419775063942 1103 62-63 0.9166871372068073 1103 64-65 0.9262616697516108 1103 66-67 0.8303891553029814 1103 68-69 0.8061995196643323 1103 70-71 1.0352111657841903 1103 72-73 0.7493046342720433 1103 74-75 0.8996844055497988 1103 76-77 0.5037592846643939 1103 78-79 0.8657126973488012 1103 80-81 1.0494798077523377 1103 82-83 1.1191377240965679 1103 84-85 1.3767060035955403 1103 86-87 1.4859109301147306 1103 88-89 1.65968396687067 1103 90-91 1.580411653900299 1103 92-93 1.8955207932215892 1103 94-95 1.9263846118393317 1103 96-97 1.9677314386697091 1103 98-99 2.3594078302676067 1103 100-101 2.491399272707614 1104 1 0.12721036756808957 1104 2 0.11926165832876023 1104 3 0.1699918130637883 1104 4 0.09854165135285342 1104 5 0.045174757769167684 1104 6 0.10106708190755143 1104 7 0.09725292666946217 1104 8 0.08929991905397827 1104 9 0.1553322082151567 1104 10-11 0.12981476353512278 1104 12-13 0.11671674176511715 1104 14-15 0.20732521499257928 1104 16-17 0.18898585693602143 1104 18-19 0.2549567794356662 1104 20-21 0.25665161666983494 1104 22-23 0.23139446030747735 1104 24-25 0.20397238332904521 1104 26-27 0.1835838127839935 1104 28-29 0.09725577135883157 1104 30-31 0.23114834437124188 1104 32-33 0.2611137723008099 1104 34-35 0.3319478882793234 1104 36-37 0.24050310072320968 1104 38-39 0.2695305969821504 1104 40-41 0.28815642642143047 1104 42-43 0.2198362094204782 1104 44-45 0.09233212856557316 1104 46-47 0.1133515926059161 1104 48-49 0.12381426203208434 1104 50-51 0.20455151569255037 1104 52-53 0.25367799572013894 1104 54-55 0.35457375495084165 1104 56-57 0.45196275721374946 1104 58-59 0.5603288333632648 1104 60-61 0.5178473096022671 1104 62-63 0.460007427376965 1104 64-65 0.5539659638112582 1104 66-67 0.39791364942470864 1104 68-69 0.6803448766185198 1104 70-71 0.7698786226861856 1104 72-73 0.7257469059875348 1104 74-75 0.8311884267612832 1104 76-77 0.6246308622731327 1104 78-79 0.8670290059373222 1104 80-81 0.9466313961120569 1104 82-83 0.9248298316470915 1104 84-85 1.0867055430923251 1104 86-87 1.180792791114495 1104 88-89 1.3151880429883072 1104 90-91 1.2954281323879329 1104 92-93 1.575118928892035 1104 94-95 1.7094763064424576 1104 96-97 1.853110528015744 1104 98-99 2.0877368367894817 1104 100-101 2.3348413526943688 1105 1 0.08403752680471399 1105 2 0.08069091694138564 1105 3 0.13824658951936897 1105 4 0.03778981935652581 1105 5 -0.014567952098083481 1105 6 0.035618686654601106 1105 7 0.008158580701199014 1105 8 -0.009382175819915517 1105 9 -0.013207433786831757 1105 10-11 0.03944322905312703 1105 12-13 -0.0036777253313147185 1105 14-15 0.11398334417897615 1105 16-17 0.10847608360914052 1105 18-19 0.17859763848081656 1105 20-21 0.12021244269952547 1105 22-23 0.09085012726936981 1105 24-25 0.1398811526589725 1105 26-27 0.11685404178856373 1105 28-29 0.09165133316433582 1105 30-31 0.09218033546886062 1105 32-33 0.023529575505087053 1105 34-35 0.09487671075020643 1105 36-37 0.1479692813140332 1105 38-39 0.06936992464050462 1105 40-41 0.23770216234866126 1105 42-43 0.10172930913547873 1105 44-45 0.2809672025354786 1105 46-47 0.42286668471825095 1105 48-49 0.3297656205030819 1105 50-51 0.4735306324753594 1105 52-53 0.44252954941649136 1105 54-55 0.54040212948874 1105 56-57 0.40872007347480377 1105 58-59 0.534933709709378 1105 60-61 0.6210402222653855 1105 62-63 0.6509891080836852 1105 64-65 0.604519130227132 1105 66-67 0.6015258544260647 1105 68-69 0.6807851218494392 1105 70-71 0.7554279937979231 1105 72-73 0.6808781538353017 1105 74-75 0.6182201940933965 1105 76-77 0.5731421014650557 1105 78-79 0.8830503480747787 1105 80-81 1.0865229401878302 1105 82-83 1.0321485882880381 1105 84-85 1.200643470943362 1105 86-87 1.3351437572589084 1105 88-89 1.583781013535674 1105 90-91 1.3700535931505868 1105 92-93 1.4962404998866674 1105 94-95 1.6361470442120627 1105 96-97 1.8160966820226925 1105 98-99 1.9553359937160941 1105 100-101 2.289191766124585 1106 1 0.05893078544285402 1106 2 0.10562315853591286 1106 3 0.1540549398436113 1106 4 0.09419731471292181 1106 5 -0.0024926330081669335 1106 6 0.029968264290630486 1106 7 -0.046255048232907825 1106 8 -0.0018864629729691273 1106 9 -0.11698829448384629 1106 10-11 0.04725043289607811 1106 12-13 0.027500943635665465 1106 14-15 0.02664386128157048 1106 16-17 0.05013151121620041 1106 18-19 0.01750162439899583 1106 20-21 -0.016094234889720838 1106 22-23 0.11134663986640447 1106 24-25 0.10652771755732715 1106 26-27 0.05861219256414074 1106 28-29 0.022234855849625035 1106 30-31 0.07094070013934584 1106 32-33 0.12641330972881093 1106 34-35 0.0395445681847022 1106 36-37 0.15450913401484456 1106 38-39 0.12969361927854806 1106 40-41 0.02567198312427621 1106 42-43 0.10868961392191778 1106 44-45 0.1241203469013854 1106 46-47 -0.09461715501546308 1106 48-49 0.1824605968540638 1106 50-51 0.15769504592930872 1106 52-53 0.08902526355962692 1106 54-55 0.17247026119733988 1106 56-57 0.32037134354794716 1106 58-59 0.24844065599909726 1106 60-61 0.3200077086002935 1106 62-63 0.29508395170467594 1106 64-65 0.30338105797482484 1106 66-67 0.4309875720611416 1106 68-69 0.045035077454883776 1106 70-71 0.19933757926295215 1106 72-73 0.21846146653759035 1106 74-75 0.3585485809443121 1106 76-77 0.513354706019097 1106 78-79 0.6681761628930936 1106 80-81 0.7445707171547689 1106 82-83 0.760319509919011 1106 84-85 0.8241935998495968 1106 86-87 0.9523256168010157 1106 88-89 1.1977901205764567 1106 90-91 1.0483683758246585 1106 92-93 1.0940208855295346 1106 94-95 1.30397806881248 1106 96-97 1.4678054349439797 1106 98-99 1.6316847780829917 1106 100-101 2.015088681966766 1107 1 0.08119536327271959 1107 2 0.08464962757665973 1107 3 0.16167504027226443 1107 4 0.1046628155232483 1107 5 0.07302540013500902 1107 6 0.10583716447724356 1107 7 0.06124520389414556 1107 8 0.0764997987997944 1107 9 0.1015734164464277 1107 10-11 0.04845391936369481 1107 12-13 0.09725859676669302 1107 14-15 0.15463004237368239 1107 16-17 0.13990218895717277 1107 18-19 0.0715094282199118 1107 20-21 0.10335832496212305 1107 22-23 0.09465317085614089 1107 24-25 0.1861174657977216 1107 26-27 0.15535955812960367 1107 28-29 0.13575238395691258 1107 30-31 0.043882033887548744 1107 32-33 0.014358978498755448 1107 34-35 0.14093591862410904 1107 36-37 0.1785464972896662 1107 38-39 0.2419740439777769 1107 40-41 0.25930948461944325 1107 42-43 0.18546226255374876 1107 44-45 0.27747637297250094 1107 46-47 0.3341417466331009 1107 48-49 0.15647571822295703 1107 50-51 0.104266235273208 1107 52-53 0.18189667407172294 1107 54-55 0.21880120668551228 1107 56-57 0.3172003279176323 1107 58-59 0.39458850643029564 1107 60-61 0.42286679029492547 1107 62-63 0.49929724884637494 1107 64-65 0.37586895392180253 1107 66-67 0.2561008337877837 1107 68-69 0.5014727606378955 1107 70-71 0.7822331236111282 1107 72-73 0.40345955506271736 1107 74-75 0.4569692101752594 1107 76-77 0.6757659631597654 1107 78-79 0.8103959129237843 1107 80-81 0.9241542892595902 1107 82-83 1.0527360754091113 1107 84-85 1.1513231743920365 1107 86-87 1.1784556737074574 1107 88-89 1.365926499031282 1107 90-91 1.3902090362177262 1107 92-93 1.5006403738171556 1107 94-95 1.2830162646929182 1107 96-97 1.3919194135714257 1107 98-99 1.6398921586431712 1107 100-101 2.1376522942086496 1108 1 0.05394422037232971 1108 2 0.05114523255896586 1108 3 0.16830540621876366 1108 4 0.09646028852926491 1108 5 0.09815136202753649 1108 6 0.04654896682983889 1108 7 0.0847393653316999 1108 8 0.0366260355456518 1108 9 0.03468616429447735 1108 10-11 0.06188769590115584 1108 12-13 -0.02324659118404071 1108 14-15 0.12559920533610125 1108 16-17 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sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 14.0 5 35.0 6 77.0 7 199.0 8 541.0 9 1015.0 10 1726.0 11 2865.0 12 4570.0 13 6634.0 14 9286.0 15 12504.0 16 16155.0 17 22376.0 18 22230.0 19 16912.0 20 15236.0 21 15333.0 22 16198.0 23 17613.0 24 20258.0 25 23170.0 26 27457.0 27 32715.0 28 39437.0 29 47633.0 30 58242.0 31 71829.0 32 90155.0 33 117954.0 34 162950.0 35 241799.0 36 375275.0 37 535047.0 38 367898.0 39 27633.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75660497534467 18.826812965669244 15.257511633728187 23.15907042525789 2 15.567738481392922 19.672225958527562 40.35655919877457 24.40347636130495 3 19.60568863215396 23.401703879829547 29.854743914065768 27.13786357395073 4 12.969056306996594 16.87396581051129 36.89975704033659 33.257220842155526 5 13.734648727866327 37.01909811431111 34.46351300221564 14.782740155606922 6 32.5446287320723 36.293367875904494 16.87585244616423 14.28615094585898 7 28.62673679810391 32.816985532293145 20.07205352547607 18.48422414412688 8 26.32797681596183 35.16693267508641 19.286560461565912 19.218530047385848 9 25.893998210640497 16.971075325756782 20.173954492771102 36.96097197083162 10-11 24.963795623091166 26.56736244608584 26.980914324706788 21.487927606116205 12-13 25.915952148264047 23.74887338918404 27.528296415746034 22.806878046805878 14-15 22.482112600775388 25.317309781418913 26.041686458943452 26.158891158862247 16-17 21.974925417522467 28.475192355154068 27.006797873999243 22.543084353324225 18-19 21.990802890402144 26.855138708406272 29.469082329537898 21.684976071653686 20-21 22.323684890982072 25.98598582886751 30.0131599708878 21.67716930926262 22-23 22.22314700918389 25.902383251727194 29.800518633378744 22.073951105710172 24-25 21.998603432433452 26.051912576770427 29.907343669420356 22.042140321375765 26-27 21.632914943343625 26.494390136787537 29.649563782886705 22.22313113698213 28-29 21.785065121611353 26.875585638073655 29.491953743343835 21.847395496971153 30-31 21.925126400598767 26.378748053210828 29.398958724661796 22.297166821528613 32-33 21.5802631100654 26.378419819504252 29.483003757298974 22.558313313131382 34-35 22.349577658023733 26.523154602177673 29.25599936710155 21.871268372697042 36-37 22.37276716190281 26.119486219007122 29.231001512724315 22.276745106365752 38-39 21.842731769388955 26.49767143534398 29.180648899547922 22.47894789571914 40-41 22.474797031459865 26.35641155835136 29.114742760537403 22.054048649651367 42-43 22.05125662810129 26.33100123318316 29.083850258100895 22.53389188061465 44-45 22.086899874130594 26.609915964512016 29.214859116733027 22.08832504462436 46-47 22.528405893183724 26.86291336086405 28.405686651734403 22.202994094217825 48-49 22.186917265996122 26.868306271516435 28.41396804048956 22.53080842199788 50-51 22.41399963155386 27.203407052852196 28.22822016556945 22.154373150024494 52-53 22.78649372278476 26.818562580907386 28.235551913317675 22.159391782990177 54-55 22.319203231090906 27.046404386987405 28.32574273702611 22.308649644895574 56-57 22.330355748792428 27.205968001361434 28.163989924732896 22.29968632511324 58-59 22.388617327377613 26.902020588373787 28.01615710675603 22.693204977492567 60-61 22.053814615415753 27.132051592373003 28.310666185318496 22.50346760689275 62-63 22.145430483052717 27.152043512993508 28.404202764337388 22.298323239616387 64-65 21.8928259571132 27.16860706706133 28.253932914714632 22.684634061110838 66-67 21.77911525655181 27.209349298814985 28.195233899758488 22.816301544874726 68-69 22.31449456179508 26.886890748700885 28.307538269212777 22.491076420291257 70-71 22.132109420242564 27.048924029875533 27.864482866015305 22.954483683866602 72-73 22.144191314419214 27.124988331162037 27.889823864570435 22.840996489848315 74-75 22.137334581313834 27.451653835763896 27.71446085011925 22.696550732803026 76-77 22.17857824399891 27.020815554705578 28.004921977812273 22.795684223483235 78-79 22.257368716279124 27.290995622430277 27.870864584477843 22.58077107681276 80-81 22.4082905458112 27.290518755980287 27.701572238782358 22.599618459426154 82-83 22.371795917684533 27.356586007708945 27.773281158319996 22.498336916286533 84-85 22.352691146164435 27.44860036606478 27.659225993160348 22.53948249461044 86-87 22.45736300584768 27.493235124398947 27.430450139428103 22.61895173032527 88-89 22.35590789257645 27.571528442540256 27.224003527500773 22.848560137382524 90-91 22.229905397802614 27.571116877623535 27.39145036048964 22.80752736408421 92-93 22.37088473690363 27.170866727496605 27.618871380202126 22.83937715539764 94-95 22.15874004585752 27.17985342281236 27.560980546283588 23.10042598504653 96-97 22.44456198229676 26.826153737170173 27.63907186769598 23.09021241283709 98-99 22.12488751928204 27.061420203178848 27.354691021637812 23.459001255901303 100-101 22.44736441538931 26.773580810061652 27.459850399508117 23.31920437504092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 674.0 1 451.0 2 360.0 3 493.0 4 409.5 5 383.5 6 751.5 7 1773.5 8 3026.0 9 3486.5 10 2893.0 11 2206.0 12 2074.5 13 2199.5 14 2427.5 15 2618.5 16 2770.5 17 3010.5 18 3295.0 19 3676.0 20 4201.5 21 4974.0 22 6040.5 23 7492.0 24 9436.0 25 12140.0 26 15704.0 27 19712.5 28 23103.5 29 26185.5 30 29838.5 31 35235.0 32 42293.5 33 45340.5 34 46174.0 35 49284.5 36 53443.0 37 57773.0 38 60985.5 39 63725.5 40 68284.5 41 73891.0 42 76805.0 43 78970.5 44 82323.0 45 86080.5 46 88248.0 47 90423.5 48 90871.0 49 88191.5 50 87157.5 51 85824.0 52 83639.0 53 83542.0 54 84341.0 55 79426.5 56 72323.0 57 66383.0 58 61567.0 59 54856.0 60 43590.0 61 35498.5 62 29734.5 63 21927.0 64 15133.0 65 11120.5 66 8050.5 67 5884.0 68 4417.0 69 3306.5 70 2379.0 71 1549.5 72 1083.5 73 824.0 74 571.0 75 384.5 76 267.0 77 174.0 78 101.5 79 49.5 80 26.5 81 17.5 82 10.0 83 3.0 84 0.5 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011813407639392013 3 0.006195843167513294 4 0.0 5 2.4783372670053176E-4 6 1.6522248446702119E-4 7 0.0 8 8.261124223351059E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0652810558377648E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017554888974621 26-27 0.021024561148428444 28-29 3.097921583756647E-4 30-31 1.858752950253988E-4 32-33 0.003717505900507976 34-35 0.015221121381524327 36-37 0.007435011801015952 38-39 0.02046693526335225 40-41 0.021850673570763552 42-43 0.017059221521219935 44-45 0.008880708540102387 46-47 0.0018794057608123658 48-49 3.5109777949242E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0652810558377646E-4 68-69 1.858752950253988E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4456967390864353E-4 82-83 9.706820962437495E-4 84-85 0.003924034006091754 86-87 7.0219555898484E-4 88-89 2.0652810558377648E-5 90-91 0.004295784596142551 92-93 8.674180434518613E-4 94-95 4.1305621116755296E-5 96-97 3.5109777949242E-4 98-99 3.097921583756647E-4 100-101 0.0026229069409139613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2420978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.238847229553876 #Duplication Level Percentage of deduplicated Percentage of total 1 75.03790222333734 41.45007217341133 2 14.29417844930638 15.79187879266433 3 4.7737112379776505 7.91082917377956 4 2.104425986952849 4.649842623967664 5 1.0754825829591939 2.970420904906445 6 0.6298107232557103 2.087401099527421 7 0.4234424305018531 1.6373330210301997 8 0.28766553074496964 1.271224984482393 9 0.22034185977214318 1.095428729720628 >10 1.041358537399024 10.393978570090177 >50 0.06882218165248827 2.5926067933041503 >100 0.03732078491507279 3.861529474139959 >500 0.0027687356126484372 1.0433521765302751 >1k 0.002544243535945525 2.266100908114315 >5k 2.2449207670107568E-4 0.9780005743311936 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9976 0.41206487626075083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8308 0.343167100238003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5376 0.22205901912367648 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2995 0.12371033524468211 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2822 0.11656446279148344 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2669 0.11024470276061989 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2632 0.10871639477929994 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2460 0.10161182794721803 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.1305621116755296E-5 0.0 0.0 0.0 0.0 3 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 4 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 5 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 6 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 7 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 8 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 9 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 10-11 4.1305621116755296E-5 0.0 0.0 4.1305621116755296E-5 0.0 12-13 4.1305621116755296E-5 0.0 0.0 6.195843167513294E-5 2.4783372670053176E-4 14-15 1.6522248446702119E-4 0.0 0.0 8.261124223351059E-5 2.684865372589094E-4 16-17 1.6522248446702119E-4 0.0 0.0 1.2391686335026588E-4 3.3044496893404237E-4 18-19 2.271809161421541E-4 0.0 0.0 1.2391686335026588E-4 3.3044496893404237E-4 20-21 2.8913934781728707E-4 0.0 0.0 1.2391686335026588E-4 3.717505900507976E-4 22-23 2.8913934781728707E-4 0.0 0.0 3.097921583756647E-4 4.956674534010635E-4 24-25 3.3044496893404237E-4 0.0 0.0 6.195843167513294E-4 4.956674534010635E-4 26-27 3.3044496893404237E-4 0.0 0.0 0.0013217798757361695 5.163202639594411E-4 28-29 5.163202639594411E-4 0.0 0.0 0.004068603680000397 5.369730745178188E-4 30-31 5.782786956345741E-4 0.0 0.0 0.012247116661117944 5.576258850761965E-4 32-33 5.782786956345741E-4 0.0 0.0 0.02932699099289626 5.782786956345741E-4 34-35 5.989315061929517E-4 0.0 0.0 0.054254933336858074 6.195843167513294E-4 36-37 6.195843167513294E-4 0.0 0.0 0.09733669616163385 6.195843167513294E-4 38-39 6.608899378680847E-4 0.0 0.0 0.16088539424976186 6.195843167513294E-4 40-41 6.608899378680847E-4 0.0 0.0 0.22410364736895583 6.195843167513294E-4 42-43 7.0219555898484E-4 0.0 0.0 0.2894491399756627 6.195843167513294E-4 44-45 7.0219555898484E-4 0.0 0.0 0.37038750455394476 6.608899378680847E-4 46-47 8.261124223351058E-4 0.0 0.0 0.46543173874359867 7.228483695432178E-4 48-49 8.674180434518611E-4 0.0 0.0 0.5642760900759941 7.848068012183507E-4 50-51 8.674180434518611E-4 0.0 0.0 0.6690891036597606 9.087236645686165E-4 52-53 8.674180434518611E-4 0.0 0.0 0.763885504122714 0.0010326405279188825 54-55 8.674180434518611E-4 0.0 0.0 0.8682441558741962 0.0010326405279188825 56-57 8.674180434518611E-4 0.0 0.0 0.9934001878579648 0.0010326405279188825 58-59 8.674180434518611E-4 0.0 0.0 1.1252683832732062 0.0010326405279188825 60-61 8.674180434518611E-4 0.0 0.0 1.2614736689057067 0.0010326405279188825 62-63 8.674180434518611E-4 0.0 0.0 1.3954897566190192 0.0010326405279188825 64-65 8.674180434518611E-4 0.0 0.0 1.533409225527865 0.0010326405279188825 66-67 8.674180434518611E-4 0.0 0.0 1.6960294558645308 0.0010326405279188825 68-69 8.674180434518611E-4 0.0 0.0 1.8688315218064764 0.0010326405279188825 70-71 8.674180434518611E-4 0.0 0.0 2.049006641117763 0.0010945989595940154 72-73 8.880708540102387E-4 0.0 0.0 2.2372570093573754 0.0011772102018275258 74-75 9.293764751269942E-4 0.0 0.0 2.4279650620534348 0.0012804742546194143 76-77 9.500292856853718E-4 0.0 0.0 2.635257321627871 0.0013630854968529247 78-79 9.500292856853718E-4 0.0 0.0 2.8621490984222078 0.00140439111796968 80-81 0.0010326405279188825 0.0 0.0 3.0987270433684238 0.00140439111796968 82-83 0.0011565573912691483 0.0 0.0 3.344825933982052 0.0014870023602031907 84-85 0.0011772102018275258 0.0 0.0 3.591028088648472 0.001528307981319946 86-87 0.0012185158229442812 0.0 0.0 3.844913088842608 0.0015696136024367013 88-89 0.0012391686335026589 0.0 0.0 4.11949220521624 0.0016109192235534565 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4815 0.0 52.978695 1 GTATCAA 8385 0.0 42.37618 1 ATCAACG 10620 0.0 33.142864 3 TATCAAC 10785 0.0 32.81402 2 TCAACGC 10785 0.0 32.809273 4 CAACGCA 10945 0.0 32.329647 5 AACGCAG 11325 0.0 31.328737 6 TGGTATC 2090 0.0 30.002048 2 ACGCAGA 12750 0.0 27.566523 7 GTGGTAT 2350 0.0 27.289097 1 CGCAGAG 12845 0.0 27.288692 8 GCAGAGT 14250 0.0 24.264816 9 ACGACCG 100 0.0016549077 23.748186 5 TAAGGTG 1785 0.0 23.149494 5 GAGTACT 9895 0.0 22.224176 12-13 GTAAGGT 1675 0.0 22.117716 4 CAGAGTA 13660 0.0 21.435955 10-11 AGAGTAC 13185 0.0 21.199558 10-11 GTACTTT 10370 0.0 21.022987 14-15 AGTACTT 10300 0.0 20.31277 12-13 >>END_MODULE