FastQCFastQC Report
Thu 26 May 2016
SRR938225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446025
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106770.43650412403797995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86100.35199967293874757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.21442953363109535No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29690.12138060731186312No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27660.11308142803119346No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27270.11148700442554758No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26790.10952463691090647No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24700.10098016169090669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.058.0847781
GTATCAA84500.042.9354931
ATCAACG105550.034.1729973
TATCAAC108100.033.586692
TCAACGC108300.033.305264
CAACGCA109300.032.913595
AACGCAG112350.032.104676
TGGTATC19200.030.4441182
GTGGTAT21100.029.1854781
ACGCAGA127800.028.0536257
CGCAGAG129900.027.4897238
GCAGAGT143800.024.4677859
GAGTACT97300.023.37866812-13
GTGTAGC15550.023.0245131
CAGAGTA137100.022.4377710-11
GTACTTT102500.021.9207114-15
AGAGTAC130350.020.91872810-11
GGACCGA7600.020.634786
AGTACTT102800.019.86189712-13
TACATGG69300.019.8181972
Produced by FastQC (version 0.11.5)