##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938225_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2446025 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.931254177696466 33.0 31.0 34.0 30.0 34.0 2 32.12401549452684 34.0 31.0 34.0 30.0 34.0 3 32.19566807371143 34.0 31.0 34.0 30.0 34.0 4 35.70670373360861 37.0 35.0 37.0 35.0 37.0 5 35.728807759528216 37.0 35.0 37.0 35.0 37.0 6 35.75111129281181 37.0 36.0 37.0 35.0 37.0 7 35.70094663791253 37.0 36.0 37.0 35.0 37.0 8 35.67914269069204 37.0 35.0 37.0 33.0 37.0 9 37.35419711572858 39.0 37.0 39.0 34.0 39.0 10-11 37.2767602538813 39.0 37.0 39.0 34.0 39.0 12-13 37.18304800645946 39.0 37.0 39.0 34.0 39.0 14-15 38.45479686430024 40.0 38.0 41.0 34.0 41.0 16-17 38.377494915219586 40.0 38.0 41.0 34.0 41.0 18-19 38.32084300037816 40.0 38.0 41.0 34.0 41.0 20-21 38.21489845770178 40.0 38.0 41.0 33.5 41.0 22-23 38.06957042548625 40.0 38.0 41.0 33.0 41.0 24-25 38.02790466164491 40.0 38.0 41.0 33.0 41.0 26-27 38.0416614711624 40.0 38.0 41.0 33.0 41.0 28-29 37.95093529297534 40.0 38.0 41.0 33.0 41.0 30-31 37.76239817663352 40.0 38.0 41.0 33.0 41.0 32-33 37.534605942294135 40.0 37.0 41.0 32.5 41.0 34-35 37.32903915536431 40.0 37.0 41.0 31.5 41.0 36-37 37.108868470273194 40.0 37.0 41.0 31.0 41.0 38-39 36.86367943908995 40.0 36.0 41.0 30.5 41.0 40-41 36.640265941680894 39.5 36.0 41.0 30.0 41.0 42-43 36.35170102513261 39.0 35.5 41.0 30.0 41.0 44-45 36.12469394221237 39.0 35.0 41.0 29.0 41.0 46-47 35.98659007982339 39.0 35.0 41.0 28.5 41.0 48-49 35.72306701689476 39.0 35.0 41.0 27.0 41.0 50-51 35.078063184145705 38.5 34.5 40.5 26.5 40.5 52-53 35.14772866180844 38.5 35.0 40.0 26.5 41.0 54-55 35.28563403889985 39.0 35.0 40.5 26.0 41.0 56-57 35.07920871618238 39.0 35.0 40.5 26.0 41.0 58-59 34.814137427050014 38.0 35.0 40.0 25.5 41.0 60-61 34.448242147974774 38.0 34.0 40.0 24.5 41.0 62-63 34.03599533937715 37.5 34.0 40.0 23.0 41.0 64-65 33.611010925889964 37.0 33.5 40.0 22.0 41.0 66-67 33.190091883770606 36.0 33.0 39.0 20.5 41.0 68-69 32.706053699369384 36.0 32.5 39.0 18.5 41.0 70-71 32.22486380965035 35.0 32.0 38.5 17.5 40.0 72-73 31.744436177062784 35.0 31.5 38.0 12.0 40.0 74-75 31.224894267229487 35.0 31.0 37.0 7.5 39.0 76-77 30.688588424075796 35.0 30.5 36.5 6.5 39.0 78-79 30.126138530881736 34.5 30.0 36.0 2.0 38.5 80-81 29.602642859333002 34.0 29.5 35.5 2.0 37.0 82-83 29.064610746006274 34.0 29.0 35.0 2.0 37.0 84-85 28.57066587626864 34.0 28.5 35.0 2.0 36.5 86-87 28.079814597152524 34.0 27.5 35.0 2.0 36.0 88-89 27.536510052023182 33.5 26.5 35.0 2.0 35.5 90-91 26.937500229964943 33.0 25.5 35.0 2.0 35.0 92-93 26.333281139808467 32.5 24.5 35.0 2.0 35.0 94-95 25.737591602702345 32.0 23.0 35.0 2.0 35.0 96-97 24.9130611911162 32.0 18.0 34.0 2.0 35.0 98-99 23.844285524473378 31.5 2.0 34.0 2.0 35.0 100-101 20.932735765170023 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38517563267208743 1101 2 0.26605181210216955 1101 3 0.2362485996045791 1101 4 0.20914800441486392 1101 5 0.26667531236266484 1101 6 0.25243751774859646 1101 7 0.22791377220414688 1101 8 0.2524976163029038 1101 9 0.21171907208640306 1101 10-11 0.31998799762067165 1101 12-13 0.20636622157693552 1101 14-15 0.2694994932724839 1101 16-17 0.42585907097393516 1101 18-19 0.3495325708025092 1101 20-21 0.418603141768493 1101 22-23 0.45758186951552915 1101 24-25 0.45639207410696514 1101 26-27 0.36811803619462324 1101 28-29 0.3494000424065007 1101 30-31 0.357701560095407 1101 32-33 0.4146260600479721 1101 34-35 0.37612174605772 1101 36-37 0.3556518746530841 1101 38-39 0.30907071790072393 1101 40-41 0.36599153947855 1101 42-43 0.4059163753321613 1101 44-45 0.3178800829777515 1101 46-47 0.24868679488047007 1101 48-49 0.3170104774453577 1101 50-51 0.3377668194221073 1101 52-53 0.46552685544786243 1101 54-55 0.3887720751431729 1101 56-57 0.30086444445088745 1101 58-59 0.1762419154897117 1101 60-61 0.22524097946921984 1101 62-63 0.28193921501841146 1101 64-65 0.2572754813297564 1101 66-67 0.17740538221454472 1101 68-69 0.27209829305672173 1101 70-71 0.3609919760407294 1101 72-73 0.3138512301380807 1101 74-75 0.42200876957961597 1101 76-77 0.408952868410303 1101 78-79 0.47238080723397147 1101 80-81 0.4916002007785565 1101 82-83 0.4361693352439673 1101 84-85 0.5040016429105592 1101 86-87 0.45152483726742787 1101 88-89 0.4940826109431775 1101 90-91 0.5785247412529202 1101 92-93 0.8121258153112727 1101 94-95 0.8216941053018729 1101 96-97 0.7483094147524731 1101 98-99 0.8850482229470593 1101 100-101 0.7388232347874428 1102 1 0.3614176941800977 1102 2 0.2492777319843711 1102 3 0.17832225611229546 1102 4 0.19091610490916366 1102 5 0.1760396042122423 1102 6 0.3008300398921335 1102 7 0.15587441840640537 1102 8 0.13033320626495026 1102 9 0.2671259764606546 1102 10-11 0.28786163862226744 1102 12-13 0.21198993690413204 1102 14-15 0.2113362846535196 1102 16-17 0.19639933712975477 1102 18-19 0.3068077183395701 1102 20-21 0.35298448119194603 1102 22-23 0.24575088377200416 1102 24-25 0.13776269294152854 1102 26-27 0.21909660051112922 1102 28-29 0.3632511580229334 1102 30-31 0.3287380831107001 1102 32-33 0.41327660035076264 1102 34-35 0.28806896664691095 1102 36-37 0.47061814884400377 1102 38-39 0.44340376858314556 1102 40-41 0.4344861578173962 1102 42-43 0.6439823105251321 1102 44-45 0.5654465685158527 1102 46-47 0.6673871134884877 1102 48-49 0.6527579425916912 1102 50-51 0.6016666205897039 1102 52-53 0.5742931239003042 1102 54-55 0.5968593098832358 1102 56-57 0.5351466423883267 1102 58-59 0.4334392882403293 1102 60-61 0.4992060223277619 1102 62-63 0.6163811748209298 1102 64-65 0.5104629350479613 1102 66-67 0.5580995313406802 1102 68-69 0.5058402187335744 1102 70-71 0.5274440599959931 1102 72-73 0.6115296466580808 1102 74-75 0.5612195325356168 1102 76-77 0.48947815956211116 1102 78-79 0.6040549247740188 1102 80-81 0.7703959491716326 1102 82-83 0.9782907310632964 1102 84-85 1.020033077598967 1102 86-87 0.871408260554567 1102 88-89 0.855223788052971 1102 90-91 0.8263616681598869 1102 92-93 0.784445561255108 1102 94-95 0.6708818349557362 1102 96-97 0.5746085253483422 1102 98-99 0.7756656778312738 1102 100-101 0.7482065735235004 1103 1 0.3600278308747562 1103 2 0.3343182820479953 1103 3 0.2255772174237265 1103 4 0.1102825243412866 1103 5 0.14893511887228783 1103 6 0.2015271615376264 1103 7 0.1934846121973024 1103 8 0.16449377028195755 1103 9 0.3107263872407344 1103 10-11 0.22655122512816206 1103 12-13 0.18610148418881067 1103 14-15 0.29881515708878936 1103 16-17 0.2493172874841818 1103 18-19 0.401683682620785 1103 20-21 0.431014414483208 1103 22-23 0.4074200650197142 1103 24-25 0.4052390312422318 1103 26-27 0.35709538115441575 1103 28-29 0.4629837247023474 1103 30-31 0.49677447297009536 1103 32-33 0.45864063972995694 1103 34-35 0.40531727194443334 1103 36-37 0.5151370856337678 1103 38-39 0.42856637163990285 1103 40-41 0.5132473922850735 1103 42-43 0.5250738146825924 1103 44-45 0.5112719142414193 1103 46-47 0.6103991983381931 1103 48-49 0.6249944062851256 1103 50-51 0.6161863708053872 1103 52-53 0.6855429997355316 1103 54-55 0.6260590513076494 1103 56-57 0.6014169985671955 1103 58-59 0.6295196117022712 1103 60-61 0.647080106343715 1103 62-63 0.486286801772863 1103 64-65 0.6444766131174475 1103 66-67 0.6420462582008639 1103 68-69 0.6740491682956886 1103 70-71 0.7299037152441841 1103 72-73 0.7222037694681838 1103 74-75 0.6607593993996979 1103 76-77 0.6065816711373948 1103 78-79 0.6273462806376813 1103 80-81 0.8271992792121523 1103 82-83 0.7510936403123409 1103 84-85 0.7706647536552325 1103 86-87 0.6323541184568846 1103 88-89 0.564089172322408 1103 90-91 0.6253955098061965 1103 92-93 0.7545424801698424 1103 94-95 0.7346269534226195 1103 96-97 0.8683722079151366 1103 98-99 1.1868265656543677 1103 100-101 1.1017846307339347 1104 1 0.3364950150635835 1104 2 0.2938403921330348 1104 3 0.16534606703794452 1104 4 0.14280603907310052 1104 5 0.11290423140567896 1104 6 0.24316390349522976 1104 7 0.12926298801819058 1104 8 0.2684876394379643 1104 9 0.15301031484865035 1104 10-11 0.32597667071414804 1104 12-13 0.2550815984353889 1104 14-15 0.2876684028709704 1104 16-17 0.4237687149758429 1104 18-19 0.43424115097933225 1104 20-21 0.30408994506398557 1104 22-23 0.4650301430466328 1104 24-25 0.49057741234806684 1104 26-27 0.39638258750166955 1104 28-29 0.44147585521123034 1104 30-31 0.4169784678357118 1104 32-33 0.40086955877196573 1104 34-35 0.5087145537351674 1104 36-37 0.5446751723764294 1104 38-39 0.5246117705913278 1104 40-41 0.585486408840417 1104 42-43 0.6936472050777027 1104 44-45 0.5959263327523487 1104 46-47 0.6752163026990985 1104 48-49 0.6383415954046185 1104 50-51 0.6095130991741584 1104 52-53 0.6990224050156115 1104 54-55 0.66504934795163 1104 56-57 0.6551541487802552 1104 58-59 0.7318485536998836 1104 60-61 0.6139032610932702 1104 62-63 0.6727222909892561 1104 64-65 0.6540063258623832 1104 66-67 0.7447120607723363 1104 68-69 0.8014858946051007 1104 70-71 0.6816237801399083 1104 72-73 0.7852734615014079 1104 74-75 0.8259600321181466 1104 76-77 0.879899416557528 1104 78-79 0.8222766351061637 1104 80-81 0.8504276195662612 1104 82-83 1.0217529459311478 1104 84-85 0.8803518525318808 1104 86-87 0.8354093543644687 1104 88-89 0.8562969441599435 1104 90-91 0.9619181159094339 1104 92-93 0.7915859791944122 1104 94-95 0.8434511873070072 1104 96-97 0.7856792572047304 1104 98-99 1.179190597987077 1104 100-101 1.1651662717127138 1105 1 0.39123139056194844 1105 2 0.4183895479671662 1105 3 0.3404525525879052 1105 4 0.22543934328586346 1105 5 0.18999295657162918 1105 6 0.16939181836198713 1105 7 0.2118674878789122 1105 8 0.23168412910160185 1105 9 0.34981510154552353 1105 10-11 0.3272939092564471 1105 12-13 0.23107429213387576 1105 14-15 0.3100073852494276 1105 16-17 0.33856397553881123 1105 18-19 0.36961004935085384 1105 20-21 0.47071544622952644 1105 22-23 0.35680098114116277 1105 24-25 0.46030628060437806 1105 26-27 0.32338778867075035 1105 28-29 0.35036251526037177 1105 30-31 0.4125128446225119 1105 32-33 0.5994584042435136 1105 34-35 0.554246731387309 1105 36-37 0.5015905884828911 1105 38-39 0.4894670114741899 1105 40-41 0.5082026074938568 1105 42-43 0.5588230884183574 1105 44-45 0.6350184092270368 1105 46-47 0.5367994899265227 1105 48-49 0.5584977425896795 1105 50-51 0.4848971931349837 1105 52-53 0.38475105594567083 1105 54-55 0.39252197303711256 1105 56-57 0.5899620522115114 1105 58-59 0.45672637236527436 1105 60-61 0.4273947366436417 1105 62-63 0.5285907465154267 1105 64-65 0.5572496614063525 1105 66-67 0.694052191012851 1105 68-69 0.7609147922694461 1105 70-71 0.7813781669823499 1105 72-73 0.7466444106737988 1105 74-75 0.7434600558343121 1105 76-77 0.7274826538044898 1105 78-79 0.7677962216343523 1105 80-81 0.8432128193037443 1105 82-83 0.8426197154540773 1105 84-85 0.9978251416697965 1105 86-87 0.9780584233543408 1105 88-89 0.9994493993859912 1105 90-91 0.7494940399556356 1105 92-93 0.964412005220094 1105 94-95 1.0173064083545356 1105 96-97 1.1158668218312613 1105 98-99 1.2481688282787324 1105 100-101 1.1384325558736812 1106 1 0.06958158901132094 1106 2 -0.027222049138600823 1106 3 0.02620872703515431 1106 4 0.028409194440435215 1106 5 -4.3610407893623915E-4 1106 6 -0.0026384145009572535 1106 7 0.11637385136556588 1106 8 0.09197946747374885 1106 9 0.08157943577392501 1106 10-11 -0.4231741523250321 1106 12-13 -0.26382214787593483 1106 14-15 -0.102316051940754 1106 16-17 0.06780753223550562 1106 18-19 0.11225280351223432 1106 20-21 0.10596062614462909 1106 22-23 -0.05241162350265682 1106 24-25 -0.009693416932556431 1106 26-27 0.037156159100618424 1106 28-29 -0.1267201949430543 1106 30-31 0.08483058542511657 1106 32-33 -0.030837555858248322 1106 34-35 -0.1630942318567108 1106 36-37 -0.22130796540762532 1106 38-39 0.05556905242769261 1106 40-41 0.2258094861759261 1106 42-43 0.07658657575019134 1106 44-45 0.06577251684339558 1106 46-47 0.1861059253321713 1106 48-49 -0.007351153588189163 1106 50-51 0.03974508782406616 1106 52-53 -0.09221839864621728 1106 54-55 -0.32633970724599237 1106 56-57 -0.18143200002050008 1106 58-59 0.0149256417489525 1106 60-61 -0.2441189565311177 1106 62-63 -0.14037251094448777 1106 64-65 -0.07769834855476887 1106 66-67 -0.36473948587594407 1106 68-69 -0.6629872668318342 1106 70-71 -0.25967371551411134 1106 72-73 -0.028790379971351143 1106 74-75 -0.045849303380897766 1106 76-77 -0.10420749295730758 1106 78-79 0.0039038413509118186 1106 80-81 0.09732640090323486 1106 82-83 0.07212624697577041 1106 84-85 0.13255672250976858 1106 86-87 0.1177302341334503 1106 88-89 0.09109192501973595 1106 90-91 0.025729601545513958 1106 92-93 0.1808603663744286 1106 94-95 0.016450521919331607 1106 96-97 0.10653625659502097 1106 98-99 -0.30142629131180243 1106 100-101 -0.3241733482051963 1107 1 0.1289337507392787 1107 2 0.0398987685526393 1107 3 0.0750329135154928 1107 4 -5.420323032723218E-4 1107 5 0.10518433218436485 1107 6 0.011947991620438358 1107 7 0.02343330836513502 1107 8 0.08499184554687389 1107 9 0.040186512343467484 1107 10-11 0.08337570503909575 1107 12-13 0.08639859139847061 1107 14-15 0.12457598107329204 1107 16-17 0.1922275549143322 1107 18-19 0.258640427068876 1107 20-21 0.2413611192115468 1107 22-23 0.23673856946791005 1107 24-25 0.2743600744964354 1107 26-27 0.35060945470696225 1107 28-29 0.3111155133564054 1107 30-31 0.30229293312817873 1107 32-33 0.3774265959958356 1107 34-35 0.42479818916702783 1107 36-37 0.35367044019225347 1107 38-39 0.39413939344370164 1107 40-41 0.411828005332886 1107 42-43 0.3706175262610927 1107 44-45 0.4864076115986222 1107 46-47 0.48734067683359683 1107 48-49 0.45666329867564315 1107 50-51 0.4396609198102297 1107 52-53 0.46213523927367817 1107 54-55 0.3853923673646946 1107 56-57 0.35463190185253524 1107 58-59 0.29376482207693044 1107 60-61 0.18957244476053603 1107 62-63 0.28040863592188003 1107 64-65 0.36940861299090244 1107 66-67 0.3508509031429412 1107 68-69 0.3358572447116117 1107 70-71 0.36067001154771106 1107 72-73 0.4171996433531113 1107 74-75 0.46601484496291334 1107 76-77 0.4742579851502313 1107 78-79 0.26116938540129553 1107 80-81 0.4039359327780119 1107 82-83 0.470385045675215 1107 84-85 0.52605958180893 1107 86-87 0.5870839723466368 1107 88-89 0.5963251194677532 1107 90-91 0.6816855972354148 1107 92-93 0.7380816873499576 1107 94-95 0.6931158294029061 1107 96-97 0.6967857267643467 1107 98-99 0.4985819045309192 1107 100-101 0.4713763405408251 1108 1 0.4145630932004565 1108 2 0.341598143874549 1108 3 0.3368652385353883 1108 4 0.25010944943122126 1108 5 0.2745101528922689 1108 6 0.3520937938629416 1108 7 0.2835666182465246 1108 8 0.3219284436626353 1108 9 0.40530743191571617 1108 10-11 0.39258730558609756 1108 12-13 0.2683588955853651 1108 14-15 0.4033301727076761 1108 16-17 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-0.9181715040895 2316 54-55 -1.1281120919221976 2316 56-57 -1.0402405352115025 2316 58-59 -1.1949185434164065 2316 60-61 -1.1050284223255673 2316 62-63 -1.1491646125614494 2316 64-65 -1.0592916993465664 2316 66-67 -1.2767988693909231 2316 68-69 -1.2629243923681592 2316 70-71 -1.4759707290677682 2316 72-73 -1.34745677172209 2316 74-75 -1.3562631253280095 2316 76-77 -1.3975337459964052 2316 78-79 -1.3529782130188295 2316 80-81 -1.374153187146959 2316 82-83 -1.299076174479282 2316 84-85 -1.367939005039986 2316 86-87 -1.472570929943931 2316 88-89 -1.4169343692567473 2316 90-91 -1.4285520699135787 2316 92-93 -1.4464908344483085 2316 94-95 -1.4049843690415322 2316 96-97 -1.567045740801273 2316 98-99 -1.5071940991399835 2316 100-101 -1.3598133480266341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6873.0 3 1801.0 4 1744.0 5 2011.0 6 2220.0 7 2822.0 8 3359.0 9 4400.0 10 5537.0 11 6958.0 12 8815.0 13 11143.0 14 13026.0 15 16053.0 16 19381.0 17 25352.0 18 23018.0 19 18306.0 20 17790.0 21 18038.0 22 19302.0 23 20965.0 24 23555.0 25 26145.0 26 29462.0 27 34301.0 28 40070.0 29 47465.0 30 56523.0 31 69364.0 32 86953.0 33 113202.0 34 155986.0 35 229763.0 36 351362.0 37 494109.0 38 390263.0 39 48582.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.415788269103786 18.542642806631733 14.88038959720885 23.16117932705563 2 15.35341214660217 19.413800748210665 40.96600656259127 24.266780542595896 3 19.38030729382062 23.424844076672514 29.686607099394553 27.508241530112315 4 12.714953488961964 16.511602599610697 37.12676415888619 33.64667975254115 5 13.760126675504491 36.98769250912424 34.504181600955796 14.747999214415476 6 32.47399326077872 36.0872079626441 17.124768091141974 14.314030685435203 7 28.590364654608475 32.86939929542575 20.170370979494034 18.369865070471743 8 26.288717354872936 35.08344660386782 19.43153803456159 19.196298006697653 9 26.019519773167815 17.079290233200794 20.24968882973393 36.65150116389746 10-11 24.984861763350903 26.513332695072545 27.03118313093102 21.470622410645536 12-13 25.969265576864913 23.807861355803315 27.49659377265105 22.72627929468072 14-15 22.59171736566725 25.32731585658029 26.05929993451024 26.02166684324222 16-17 22.05062448123529 28.43755126285074 26.96645829489685 22.54536596101712 18-19 22.041488045479397 26.806728722082372 29.45418464980361 21.697598582634615 20-21 22.448225601055587 25.9705928983128 29.947271788105212 21.633909712526403 22-23 22.251520572084885 25.908789198134098 29.756125499485048 22.083564730295972 24-25 22.020540665070374 26.049124995166313 29.842950683294138 22.08738365646917 26-27 21.613874588925047 26.47636685937611 29.615724566843543 22.2940339848553 28-29 21.809052051423038 26.860615819652818 29.426531039719322 21.903801089204826 30-31 21.955900002475207 26.38975174496945 29.37237559069985 22.28197266185549 32-33 21.596086147788586 26.35306290315421 29.43029565613709 22.620555292920113 34-35 22.368348646843486 26.53829434465575 29.18145767978586 21.911899328714902 36-37 22.475248739935854 26.10466305557374 29.13284676310794 22.28724144138247 38-39 21.945532485050208 26.52053135008877 29.079593311621142 22.454342853239876 40-41 22.554662364954574 26.337938532089826 29.05905228723417 22.048346815721427 42-43 22.105549245530504 26.368456291567604 28.99338353995541 22.532610922946482 44-45 22.177159743013018 26.615875282432768 29.096622011058297 22.110342963495917 46-47 22.560946061250824 26.917582167049677 28.300519539523872 22.220952232175627 48-49 22.262218891627654 26.921198155268637 28.335601250131624 22.480981702972077 50-51 22.4481229130216 27.24962239888704 28.194448164624475 22.107806523466884 52-53 22.77573536928972 26.856508422038385 28.12617313535375 22.241583073318147 54-55 22.275385802863553 27.06842985007489 28.353277343570294 22.302907003491267 56-57 22.350690132211444 27.22250870102695 28.166586993298846 22.26021417346276 58-59 22.413657384831705 26.82766603059405 28.0828615948105 22.675814989763747 60-61 22.00625279873063 27.097131032734158 28.397050674660218 22.499565493875 62-63 22.142375210078878 27.024833265862746 28.55539335353362 22.277398170524755 64-65 21.903157378205552 27.105363504858488 28.377454162556166 22.614024954379794 66-67 21.84461513613196 27.104806377154116 28.298192062904683 22.75238642380924 68-69 22.34622891133222 26.78842984060547 28.438640200820505 22.426701047241806 70-71 22.157417946812412 26.882488080625972 28.06295294541567 22.89714102714595 72-73 22.139416716051414 27.016461990923023 28.0745020524832 22.76961924054236 74-75 22.12585718398702 27.338136998939973 27.931356575451822 22.604649241621193 76-77 22.292361235775175 26.907605559851493 28.039907403961873 22.76012580041146 78-79 22.30273748720681 27.17747973227425 27.988658987413096 22.531123793105845 80-81 22.470813441344845 27.254070416565888 27.693941393198042 22.581174748891225 82-83 22.362287054826744 27.279963213983923 27.816541807802064 22.541207923387276 84-85 22.388749952976237 27.3172084076854 27.782130315525364 22.511911323812996 86-87 22.533743670761865 27.34445529300938 27.54242797425129 22.57937306197747 88-89 22.449984989566317 27.382221266062594 27.402630547827663 22.76516319654343 90-91 22.355492680169238 27.351433890819486 27.477523265176895 22.815550163834377 92-93 22.47725932427005 26.98897585370063 27.755688951888164 22.77807587014115 94-95 22.344409249799103 26.902121502860215 27.77261118475239 22.98085806258829 96-97 22.59053398172418 26.565789659353488 27.802842173654035 23.040834185268295 98-99 22.336537730115797 26.72109887934722 27.507032196376386 23.4353311941606 100-101 22.777539790709714 26.17830218660516 27.76358273649999 23.280575286185137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 687.0 1 491.0 2 403.5 3 519.0 4 425.5 5 378.0 6 756.0 7 1769.0 8 3019.5 9 3534.0 10 2961.0 11 2305.5 12 2267.5 13 2379.0 14 2527.5 15 2763.5 16 2959.5 17 3203.5 18 3502.0 19 3918.0 20 4449.0 21 5190.5 22 6341.0 23 7758.0 24 9640.0 25 12453.0 26 16012.5 27 20036.5 28 23333.5 29 26265.5 30 29907.0 31 35290.5 32 42460.5 33 45670.5 34 46489.5 35 49798.0 36 54149.0 37 58240.5 38 61802.0 39 64895.5 40 68986.0 41 74036.5 42 77137.5 43 79815.5 44 83067.5 45 87202.0 46 89487.0 47 90949.5 48 91344.0 49 88998.5 50 87570.0 51 86099.5 52 84155.0 53 83321.0 54 83803.0 55 79410.5 56 72396.5 57 66700.0 58 62088.0 59 54954.0 60 43633.5 61 35681.5 62 30197.0 63 22599.0 64 16071.0 65 12405.5 66 8916.0 67 6400.5 68 5005.5 69 3845.0 70 2780.5 71 1850.0 72 1285.0 73 967.5 74 704.0 75 506.0 76 380.5 77 229.5 78 130.5 79 88.0 80 56.5 81 38.0 82 27.5 83 23.5 84 14.0 85 15.0 86 12.5 87 4.5 88 4.0 89 5.0 90 3.5 91 2.5 92 4.0 93 2.5 94 0.5 95 1.0 96 1.0 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.504164920636543 2 0.05147126460277389 3 0.05699042323770198 4 0.06782432722478307 5 0.08115207326172054 6 0.11103729520344231 7 0.06999110802219928 8 0.052656861642869555 9 0.04995860631023804 10-11 0.0769820422931082 12-13 0.11062846863789208 14-15 0.055927474167271396 16-17 0.06197810733741478 18-19 0.060894716938706676 20-21 0.0771660142476058 22-23 0.06674093682607496 24-25 0.09151582669841887 26-27 0.06833536043172085 28-29 0.06839668441655339 30-31 0.07295510062243844 32-33 0.07291421796588343 34-35 0.07491746813707956 36-37 0.06970492942631412 38-39 0.04120971780746313 40-41 0.0452571008064104 42-43 0.04341738126143437 44-45 0.021729131958994695 46-47 0.028065943725023256 48-49 0.02790241309880316 50-51 0.026716816058707498 52-53 0.02677814004354003 54-55 0.026062693553827128 56-57 0.025510777690334316 58-59 0.022465019776985107 60-61 0.029435512719616523 62-63 0.021054568125836816 64-65 0.022996494312200407 66-67 0.017170715753109637 68-69 0.018724256702200508 70-71 0.04317208532210423 72-73 0.03834793184861152 74-75 0.03307406915301356 76-77 0.02305781829703294 78-79 0.015862470743348903 80-81 0.01723203973794217 82-83 0.021688249302439674 84-85 0.018519843419425395 86-87 0.009525658977320346 88-89 0.0432947332917693 90-91 0.054394374546458034 92-93 0.03462761010210444 94-95 0.05586615018243886 96-97 0.04499136353880275 98-99 0.03446407947588434 100-101 0.17371040770229249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2446025.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.54293866822573 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26903860395277 43.12475572067841 2 13.639453648306013 15.42429582208549 3 4.529954566690555 7.6841082970269925 4 1.9418871961753599 4.392000345358247 5 1.0266044125858904 2.9023615168686954 6 0.6289668237198428 2.133817952276389 7 0.38080396231865016 1.5072242560200542 8 0.2739681718661263 1.2392772431097845 9 0.20456008368168757 1.0409785439022703 >10 1.0015938795545407 10.206026580420717 >50 0.06361377912981073 2.4782799869430066 >100 0.034191229411573786 3.5919919783563894 >500 0.0026818213036383137 1.02396672065011 >1k 0.0024643763330694807 2.2455137318822547 >5k 1.4496331370996945E-4 0.5678230504139117 >10k+ 7.248165685498472E-5 0.43757825400716965 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10677 0.43650412403797995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8610 0.35199967293874757 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.21442953363109535 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2969 0.12138060731186312 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2766 0.11308142803119346 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2727 0.11148700442554758 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2679 0.10952463691090647 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2470 0.10098016169090669 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.17653131100459E-5 2 0.0 0.0 0.0 0.0 8.17653131100459E-5 3 0.0 0.0 0.0 0.0 8.17653131100459E-5 4 0.0 0.0 0.0 4.088265655502295E-5 8.17653131100459E-5 5 4.088265655502295E-5 0.0 0.0 4.088265655502295E-5 8.17653131100459E-5 6 4.088265655502295E-5 0.0 0.0 4.088265655502295E-5 1.635306262200918E-4 7 4.088265655502295E-5 0.0 0.0 4.088265655502295E-5 1.635306262200918E-4 8 8.17653131100459E-5 0.0 0.0 4.088265655502295E-5 1.635306262200918E-4 9 8.17653131100459E-5 0.0 0.0 4.088265655502295E-5 1.635306262200918E-4 10-11 1.2264796966506884E-4 0.0 0.0 8.17653131100459E-5 1.635306262200918E-4 12-13 1.2264796966506884E-4 0.0 0.0 8.17653131100459E-5 4.088265655502294E-4 14-15 2.0441328277511473E-4 0.0 0.0 1.2264796966506884E-4 4.905918786602754E-4 16-17 2.0441328277511473E-4 0.0 0.0 1.4308929794258033E-4 5.723571917703212E-4 18-19 2.2485461105262622E-4 0.0 0.0 1.635306262200918E-4 5.927985200478328E-4 20-21 2.452959393301377E-4 0.0 0.0 2.2485461105262622E-4 6.132398483253442E-4 22-23 3.270612524401836E-4 0.0 0.0 2.452959393301377E-4 6.132398483253442E-4 24-25 4.497092221052524E-4 0.0 0.0 5.927985200478328E-4 6.132398483253442E-4 26-27 4.497092221052524E-4 0.0 0.0 8.789771159329933E-4 6.132398483253442E-4 28-29 6.132398483253442E-4 0.0 0.0 0.002861785958851606 6.132398483253442E-4 30-31 7.35887817990413E-4 0.0 0.0 0.0106908146891385 6.132398483253442E-4 32-33 7.35887817990413E-4 0.0 0.0 0.026778140043540028 6.745638331578786E-4 34-35 7.972118028229474E-4 0.0 0.0 0.05314745352152983 6.950051614353901E-4 36-37 9.403011007655277E-4 0.0 0.0 0.09349863554133747 6.950051614353901E-4 38-39 9.811837573205507E-4 0.0 0.0 0.1537392299751638 6.950051614353901E-4 40-41 9.811837573205507E-4 0.0 0.0 0.21784323545343975 6.950051614353901E-4 42-43 0.0010629490704305966 0.0 0.0 0.28213121288621335 7.154464897129015E-4 44-45 0.0011038317269856195 0.0 0.0 0.36078944409807745 7.767704745454359E-4 46-47 0.0011447143835406424 0.0 0.0 0.4528776279882667 8.380944593779704E-4 48-49 0.0012264796966506885 0.0 0.0 0.5489109882360156 8.994184442105048E-4 50-51 0.0012264796966506885 0.0 0.0 0.6522214613505586 8.994184442105048E-4 52-53 0.0012264796966506885 0.0 0.0 0.7512596968551016 0.0010220664138755736 54-55 0.0012264796966506885 0.0 0.0 0.8583722570292618 0.0010220664138755736 56-57 0.0012264796966506885 0.0 0.0 0.9814699359164358 0.0010629490704305966 58-59 0.0012264796966506885 0.0 0.0 1.1107817785999734 0.0011447143835406424 60-61 0.0012264796966506885 0.0 0.0 1.2485358898621233 0.0011447143835406424 62-63 0.0012264796966506885 0.0 0.0 1.3856154372911151 0.0011855970400956653 64-65 0.0012264796966506885 0.0 0.0 1.5248822068458008 0.0011855970400956653 66-67 0.0012264796966506885 0.0 0.0 1.682995881072352 0.0012264796966506885 68-69 0.0012264796966506885 0.0 0.0 1.8558273116587116 0.0012264796966506885 70-71 0.0012469210249282 0.0 0.0 2.0391042609948795 0.0012264796966506885 72-73 0.0012878036814832228 0.0 0.0 2.2236281313559756 0.0012878036814832228 74-75 0.0013082450097607343 0.0 0.0 2.409092302817837 0.0013695689945932687 76-77 0.0013082450097607343 0.0 0.0 2.6117271900328083 0.0013900103228707801 78-79 0.0013491276663157572 0.0 0.0 2.8406700667409366 0.0013900103228707801 80-81 0.001594423605645895 0.0 0.0 3.0784435972649504 0.0013900103228707801 82-83 0.001594423605645895 0.0 0.0 3.315154178718533 0.001430892979425803 84-85 0.0016148649339234064 0.0 0.0 3.563025725411637 0.001430892979425803 86-87 0.0016353062622009179 0.0 0.0 3.816314224098282 0.0014513343077033145 88-89 0.0016353062622009179 0.0 0.0 4.081826637094879 0.0014922169642583374 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 58.084778 1 GTATCAA 8450 0.0 42.935493 1 ATCAACG 10555 0.0 34.172997 3 TATCAAC 10810 0.0 33.58669 2 TCAACGC 10830 0.0 33.30526 4 CAACGCA 10930 0.0 32.91359 5 AACGCAG 11235 0.0 32.10467 6 TGGTATC 1920 0.0 30.444118 2 GTGGTAT 2110 0.0 29.185478 1 ACGCAGA 12780 0.0 28.053625 7 CGCAGAG 12990 0.0 27.489723 8 GCAGAGT 14380 0.0 24.467785 9 GAGTACT 9730 0.0 23.378668 12-13 GTGTAGC 1555 0.0 23.024513 1 CAGAGTA 13710 0.0 22.43777 10-11 GTACTTT 10250 0.0 21.92071 14-15 AGAGTAC 13035 0.0 20.918728 10-11 GGACCGA 760 0.0 20.63478 6 AGTACTT 10280 0.0 19.861897 12-13 TACATGG 6930 0.0 19.818197 2 >>END_MODULE