FastQCFastQC Report
Thu 26 May 2016
SRR938225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446025
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100930.4126286526098466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83790.34255577927453723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53040.2168416103678417No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29980.12256620435195878No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28390.11606586195971015No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28350.11590233133349005No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27560.11267260146564323No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24520.10024427387291626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.053.776381
GTATCAA84500.041.7712251
TATCAAC110800.031.6847152
ATCAACG111150.031.4101263
TCAACGC113000.030.9360314
CAACGCA113650.030.6755145
AACGCAG118100.029.519666
GTGGTAT23600.026.9735361
TGGTATC21900.026.6812462
ACGCAGA133450.025.9818137
CGCAGAG133750.025.8880258
GCAGAGT150500.022.5965299
GAGTACT101450.022.16833712-13
AGAGTAC134800.021.17628510-11
CAGAGTA141350.021.0686610-11
GTACATG74750.020.9088331
GTACTTT107850.020.65465214-15
TACATGG72550.020.4951952
GTAAGGT15050.020.1980134
AGTACTT106300.020.08452812-13