FastQCFastQC Report
Thu 26 May 2016
SRR938224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447355
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101440.41448829450570107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84520.3453524315025813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.20238175499672095No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29110.11894473829910249No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27860.11383718340821009No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27290.11150813837796315No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25120.10264142308737392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.057.433071
GTATCAA83000.044.178671
ATCAACG104350.034.6421433
TATCAAC105450.034.2807732
TCAACGC107500.033.58284
CAACGCA108900.033.0637135
AACGCAG112750.031.9268596
TGGTATC17850.028.5120962
ACGCAGA126650.028.1507937
CGCAGAG126350.028.064848
GTGGTAT20550.026.4175781
GCAGAGT139600.024.8222479
GAGTACT91750.022.61398912-13
CAGAGTA132600.022.3321310-11
GTACTTT97600.021.13764814-15
AGAGTAC127300.021.1149410-11
TAAGGTG15200.020.9659635
GTACATG76000.020.6776081
ACCGAGT7650.020.504618
TACATGG72850.020.1096612
Produced by FastQC (version 0.11.5)