##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2447355 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01529283655211 33.0 31.0 34.0 30.0 34.0 2 32.1369298691853 34.0 31.0 34.0 30.0 34.0 3 32.1621244159511 34.0 31.0 34.0 30.0 34.0 4 35.66768409160093 37.0 35.0 37.0 35.0 37.0 5 35.675763426229544 37.0 35.0 37.0 35.0 37.0 6 35.70255643337399 37.0 36.0 37.0 35.0 37.0 7 35.640574007448855 37.0 35.0 37.0 33.0 37.0 8 35.632860782354825 37.0 35.0 37.0 33.0 37.0 9 37.27818767608296 39.0 37.0 39.0 34.0 39.0 10-11 37.186964294105266 39.0 37.0 39.0 34.0 39.0 12-13 37.04314515058093 39.0 37.0 39.0 33.0 39.0 14-15 38.24227461892533 40.0 38.0 41.0 33.5 41.0 16-17 38.064630386682765 40.0 38.0 41.0 33.0 41.0 18-19 38.244507641923626 40.0 38.0 41.0 33.5 41.0 20-21 38.24133360301224 40.0 38.0 41.0 34.0 41.0 22-23 38.13795260597665 40.0 38.0 41.0 33.0 41.0 24-25 38.02114670736366 40.0 38.0 41.0 33.0 41.0 26-27 37.84001871408112 40.0 38.0 41.0 33.0 41.0 28-29 37.6641747927865 40.0 38.0 41.0 32.0 41.0 30-31 37.438382866400666 40.0 37.0 41.0 32.0 41.0 32-33 37.138003272921175 40.0 36.5 41.0 31.0 41.0 34-35 36.90128894255227 40.0 36.0 41.0 30.5 41.0 36-37 36.63252245791885 39.0 36.0 41.0 30.0 41.0 38-39 36.331371215046445 39.0 35.0 41.0 30.0 41.0 40-41 36.20935254591181 39.0 35.0 41.0 30.0 41.0 42-43 35.99153943747434 39.0 35.0 41.0 28.5 41.0 44-45 35.6605980742475 39.0 35.0 40.5 27.5 41.0 46-47 35.28671443252 39.0 35.0 40.0 26.0 41.0 48-49 34.87640268779968 38.0 34.5 40.0 24.0 41.0 50-51 34.143141268839216 37.5 33.5 39.5 23.5 40.5 52-53 34.19954828784545 38.0 34.0 40.0 23.0 40.5 54-55 34.27721417612075 38.0 34.0 40.0 23.0 41.0 56-57 33.96470434407759 38.0 33.5 40.0 21.5 41.0 58-59 33.556119974421364 37.5 33.0 40.0 19.5 41.0 60-61 33.04872546075253 37.0 32.5 40.0 16.0 41.0 62-63 32.50418410896662 36.5 31.5 39.5 11.5 41.0 64-65 31.959719779108468 36.0 31.0 39.0 7.5 40.5 66-67 31.384416645725693 35.0 30.5 39.0 4.5 40.0 68-69 30.69790038633545 35.0 29.5 38.0 2.0 40.0 70-71 30.104003710127873 35.0 29.0 37.5 2.0 40.0 72-73 29.407859505466106 34.0 28.0 37.0 2.0 39.0 74-75 28.725284031127483 34.0 26.5 36.0 2.0 39.0 76-77 27.975429800744067 33.5 26.0 35.5 2.0 38.5 78-79 27.20501541460066 33.0 25.0 35.0 2.0 37.0 80-81 26.47742767191519 32.5 23.5 35.0 2.0 37.0 82-83 25.72876963088722 32.0 20.5 35.0 2.0 36.0 84-85 24.988312892898662 31.0 18.0 34.5 2.0 36.0 86-87 24.237211193308696 31.0 10.0 34.0 2.0 35.0 88-89 23.349223140901096 30.5 4.5 34.0 2.0 35.0 90-91 22.44097546126328 29.5 2.0 34.0 2.0 35.0 92-93 21.490413732376382 29.0 2.0 34.0 2.0 35.0 94-95 20.399232436650998 28.0 2.0 34.0 2.0 35.0 96-97 19.120938319124114 26.0 2.0 33.5 2.0 35.0 98-99 17.53299133145784 22.5 2.0 33.0 2.0 34.5 100-101 13.562360793591449 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26059785462990703 1101 2 0.18684021241310944 1101 3 0.1402403951588269 1101 4 0.08755913991333131 1101 5 0.16247105410761264 1101 6 0.13665582935272624 1101 7 0.19042030274368926 1101 8 0.15825941955889533 1101 9 0.1535207723326124 1101 10-11 0.26560568999982337 1101 12-13 0.25729004231693864 1101 14-15 0.34654933177780833 1101 16-17 0.43924553695526214 1101 18-19 0.4063935291486942 1101 20-21 0.3677522799201114 1101 22-23 0.43156631311609317 1101 24-25 0.39247739048347086 1101 26-27 0.4090962876853155 1101 28-29 0.45293819417716463 1101 30-31 0.35712569755891366 1101 32-33 0.292565482348202 1101 34-35 0.23020068357109835 1101 36-37 0.12220613698455196 1101 38-39 0.2088388444369471 1101 40-41 0.3109419773051485 1101 42-43 0.3548404429168386 1101 44-45 0.32667604075602696 1101 46-47 0.33185127216814436 1101 48-49 0.4333248418877318 1101 50-51 0.38168344722205916 1101 52-53 0.35117524436486036 1101 54-55 0.20122255869864603 1101 56-57 0.2557906275811206 1101 58-59 0.2901928432731111 1101 60-61 0.04754471125972515 1101 62-63 -0.1407265991601534 1101 64-65 -0.1794772470566386 1101 66-67 -0.16760447000915946 1101 68-69 -0.04095393860959007 1101 70-71 -0.12568969466071778 1101 72-73 -0.013486341893671039 1101 74-75 -0.17053382490454894 1101 76-77 -0.3790445819460757 1101 78-79 -0.34017839485493084 1101 80-81 -0.23414590605154117 1101 82-83 -0.3144689050827658 1101 84-85 -0.4591554687195867 1101 86-87 -0.4158204300745716 1101 88-89 -0.44539257154480083 1101 90-91 -0.44482023344435007 1101 92-93 -0.6338600900653013 1101 94-95 -0.9382909728122293 1101 96-97 -1.082099840339911 1101 98-99 -1.133113004518492 1101 100-101 -1.2916570071467035 1102 1 0.1325881357852623 1102 2 0.1430899575295399 1102 3 0.18664809296457463 1102 4 0.1600587589294662 1102 5 0.17328713227048098 1102 6 0.10774677395659893 1102 7 0.08650958389690544 1102 8 0.18013862513778633 1102 9 0.31521998181792554 1102 10-11 0.205572721480884 1102 12-13 0.23649562120850476 1102 14-15 0.2736759192999898 1102 16-17 0.26260059888928566 1102 18-19 0.25107864330433216 1102 20-21 0.2273928887215888 1102 22-23 0.31306745392129187 1102 24-25 0.1671316610442588 1102 26-27 0.08885316632210305 1102 28-29 0.19686123329078953 1102 30-31 0.09009035298852552 1102 32-33 -0.06490330380501774 1102 34-35 -0.06608198713649927 1102 36-37 0.031003537279467253 1102 38-39 -0.1627673405945771 1102 40-41 -0.07218333526259357 1102 42-43 0.15534387821075768 1102 44-45 0.18762079487655114 1102 46-47 0.045799037670960274 1102 48-49 0.11247783427450031 1102 50-51 0.04958185438755436 1102 52-53 0.08175966287616632 1102 54-55 -0.002651671552982293 1102 56-57 0.08245005376576131 1102 58-59 0.045569545090465624 1102 60-61 0.11045062005111816 1102 62-63 0.1551842098349283 1102 64-65 0.13596848259721384 1102 66-67 0.0016363955003164676 1102 68-69 -0.22969291422447924 1102 70-71 -0.4301547181958618 1102 72-73 -0.3836748376835324 1102 74-75 -0.347928281643032 1102 76-77 -0.5238467815644476 1102 78-79 -0.8477467734090851 1102 80-81 -0.680646327816774 1102 82-83 -0.8692845410897121 1102 84-85 -0.671017481730992 1102 86-87 -0.6998379961135655 1102 88-89 -0.7811282138192013 1102 90-91 -0.7677652279603286 1102 92-93 -1.0714102887135049 1102 94-95 -1.3374110610717018 1102 96-97 -1.5420122891753891 1102 98-99 -1.295298585513514 1102 100-101 -1.5805915187039297 1103 1 0.08954780604887702 1103 2 0.1664634584025393 1103 3 0.18463303729294012 1103 4 0.12073082579267691 1103 5 0.05960822452224335 1103 6 0.061323211544255685 1103 7 0.03130163816601339 1103 8 0.006454565258579237 1103 9 0.037706171244607845 1103 10-11 0.1342343123444465 1103 12-13 0.021867862671712146 1103 14-15 0.08068424857840739 1103 16-17 0.15405807949723993 1103 18-19 0.09460842548369186 1103 20-21 0.13624024252987965 1103 22-23 0.17625690922655224 1103 24-25 -0.028340188284538215 1103 26-27 5.327610211267597E-4 1103 28-29 -0.05674525839424405 1103 30-31 0.09104922180954844 1103 32-33 0.09034185176071219 1103 34-35 -0.1171696592319833 1103 36-37 -0.1373967260516693 1103 38-39 -0.24602863950403986 1103 40-41 0.0700684322045575 1103 42-43 0.06183270094882687 1103 44-45 0.15387719926158638 1103 46-47 0.1404723066980651 1103 48-49 0.1146360046955408 1103 50-51 0.1533228739370287 1103 52-53 0.1815135055071906 1103 54-55 0.16422441969698554 1103 56-57 0.14305485669675022 1103 58-59 0.19831591713155916 1103 60-61 0.08528675366304839 1103 62-63 0.0872648120617896 1103 64-65 0.041990751252221514 1103 66-67 -0.07077108081419325 1103 68-69 -0.032848594448879 1103 70-71 -0.1496612585399113 1103 72-73 -0.09103093580300126 1103 74-75 -0.0046976092899377875 1103 76-77 -0.2311456337841591 1103 78-79 -0.15295185684498236 1103 80-81 -0.1353347670073184 1103 82-83 -0.13150224724650883 1103 84-85 -0.5796415765302001 1103 86-87 -0.5467767716237688 1103 88-89 -0.43853440210096295 1103 90-91 -0.3126030789492482 1103 92-93 -0.5069413875485509 1103 94-95 -0.8066929112591836 1103 96-97 -0.8968653103277475 1103 98-99 -1.2852361874827736 1103 100-101 -1.2700568420001481 1104 1 0.12090322671343756 1104 2 0.07161722409969684 1104 3 0.11957274625326164 1104 4 -0.03334011107779844 1104 5 0.12128647479135424 1104 6 0.06470506165916845 1104 7 0.052259080390676615 1104 8 0.16214607524759828 1104 9 0.154318799741759 1104 10-11 0.18984332562894934 1104 12-13 0.10655853720761854 1104 14-15 0.20167433105223154 1104 16-17 0.05337806795113664 1104 18-19 0.18805877436448526 1104 20-21 0.24265412775287842 1104 22-23 0.3369089935314378 1104 24-25 0.3016898132902881 1104 26-27 0.22761680851879618 1104 28-29 0.19125285716519613 1104 30-31 0.08856745707024061 1104 32-33 0.10292388612525372 1104 34-35 0.08199696580819449 1104 36-37 0.16837053143146363 1104 38-39 0.15716051493429717 1104 40-41 0.08218602937888875 1104 42-43 0.10679092638694954 1104 44-45 0.01237924061175022 1104 46-47 -0.13192935215870705 1104 48-49 -0.04560569329903075 1104 50-51 -0.08715835620380119 1104 52-53 0.008380339010265914 1104 54-55 -0.09387234491457264 1104 56-57 0.05182717151195959 1104 58-59 0.11179818939623942 1104 60-61 0.12814948273105387 1104 62-63 0.0451959731662086 1104 64-65 -0.2916649490279255 1104 66-67 -0.2763191414838708 1104 68-69 -0.2219659502531428 1104 70-71 -0.1971576827929873 1104 72-73 -0.2460142553392224 1104 74-75 -0.2885206861748806 1104 76-77 -0.42761877097348844 1104 78-79 -0.5952597398083341 1104 80-81 -0.6247227775879409 1104 82-83 -0.897632288134524 1104 84-85 -0.6006943465853674 1104 86-87 -0.74621898942193 1104 88-89 -0.6642476353960021 1104 90-91 -0.791764701184885 1104 92-93 -1.0218593350717242 1104 94-95 -1.1444307398971105 1104 96-97 -1.3748784228614923 1104 98-99 -1.5887974636402546 1104 100-101 -1.5644717941322313 1105 1 0.13488242076469703 1105 2 0.18958829154293255 1105 3 0.2880137572030108 1105 4 0.22336721688143513 1105 5 0.1702823615346034 1105 6 0.2409469959592272 1105 7 0.21855879576823867 1105 8 0.26104871987566725 1105 9 0.295876939415038 1105 10-11 0.20873393831828224 1105 12-13 0.31306119417058653 1105 14-15 0.20616304488811465 1105 16-17 0.38724849846983744 1105 18-19 0.3372012552773924 1105 20-21 0.23279540384890396 1105 22-23 0.3289711960578927 1105 24-25 0.27157471098905006 1105 26-27 0.2595148811183421 1105 28-29 0.297167550503147 1105 30-31 0.15334273013772304 1105 32-33 0.2238009589161365 1105 34-35 0.2588496039532231 1105 36-37 0.21226113961242987 1105 38-39 0.1104980705067149 1105 40-41 0.21162188257264347 1105 42-43 0.2944547267519795 1105 44-45 0.364991417431483 1105 46-47 0.3873244548444532 1105 48-49 0.4526160251078011 1105 50-51 0.447760775354233 1105 52-53 0.45341014023983206 1105 54-55 0.41432495435519456 1105 56-57 0.3707320667184888 1105 58-59 0.5052034534776411 1105 60-61 0.5881561803660205 1105 62-63 0.35206369992506126 1105 64-65 0.3560747073729402 1105 66-67 0.30282250447885417 1105 68-69 0.22936655269848671 1105 70-71 0.09201856761704974 1105 72-73 0.06963992452419632 1105 74-75 -0.09698293335223696 1105 76-77 -0.1603323716988676 1105 78-79 -0.33910955625454875 1105 80-81 -0.35148605081628403 1105 82-83 -0.18065299003007596 1105 84-85 -0.19446853962764976 1105 86-87 -0.44571654056533916 1105 88-89 -0.5115301971457953 1105 90-91 -0.6055984011088391 1105 92-93 -0.7615710085589527 1105 94-95 -0.8364076981573554 1105 96-97 -1.1468403628384891 1105 98-99 -1.3528666898470156 1105 100-101 -1.474503657490379 1106 1 0.3942060877257987 1106 2 0.29112471846752896 1106 3 0.38202798189285403 1106 4 0.3139167289164675 1106 5 0.37631636110615574 1106 6 0.2591340963116622 1106 7 0.33666480501986484 1106 8 0.12124805334399014 1106 9 0.3585927380939111 1106 10-11 0.41872301205054185 1106 12-13 0.5121242936113646 1106 14-15 0.5261433443480641 1106 16-17 -0.04510352494386893 1106 18-19 0.45434258366504565 1106 20-21 0.5296486974290104 1106 22-23 0.45379769142572 1106 24-25 0.4945144932658039 1106 26-27 0.4671657139894876 1106 28-29 0.32952426917962896 1106 30-31 0.25416498803090803 1106 32-33 0.4668923199260888 1106 34-35 0.37532821556401785 1106 36-37 0.38052820615897076 1106 38-39 0.5007567697037345 1106 40-41 0.36111454964819245 1106 42-43 0.25426914649086285 1106 44-45 0.41598382969119996 1106 46-47 0.5154525024857151 1106 48-49 0.5989268691106275 1106 50-51 0.6304687094679622 1106 52-53 0.47084208582955966 1106 54-55 0.3355047956357424 1106 56-57 0.23599328472835168 1106 58-59 0.2947467120095624 1106 60-61 0.42225816734253385 1106 62-63 0.33960677651555926 1106 64-65 0.13341351068740437 1106 66-67 0.029365118390330736 1106 68-69 -0.1386710328800156 1106 70-71 -0.14545500930260502 1106 72-73 -0.04987798937055388 1106 74-75 -0.05596218322531499 1106 76-77 -0.3212977177250629 1106 78-79 -0.2306649305207884 1106 80-81 -0.3023568753337713 1106 82-83 -0.2995322503696798 1106 84-85 -0.33757887732464553 1106 86-87 -0.4177534140289829 1106 88-89 -0.5787037565560311 1106 90-91 -0.6181336767158747 1106 92-93 -0.5026253102511085 1106 94-95 -1.009028153656537 1106 96-97 -1.060937987133343 1106 98-99 -1.10789996263102 1106 100-101 -1.1225280767695196 1107 1 -0.09202681751851571 1107 2 -0.06475640927671833 1107 3 0.018490885275909363 1107 4 0.038056340888779516 1107 5 0.02554659854932595 1107 6 -0.02622844988738393 1107 7 0.06866689671777237 1107 8 0.0035200272509428032 1107 9 -0.01810167015943165 1107 10-11 0.04442607957869171 1107 12-13 0.04508404615286565 1107 14-15 -0.0465462600905866 1107 16-17 -0.009883675085234245 1107 18-19 -0.05242751569060289 1107 20-21 4.1974423083956935E-6 1107 22-23 0.0644587034175288 1107 24-25 0.02061241308995676 1107 26-27 -0.07967901289325852 1107 28-29 -0.04912534483119657 1107 30-31 -0.10439546292498392 1107 32-33 0.02208921428648125 1107 34-35 -0.01603555374651222 1107 36-37 -0.046273889084183395 1107 38-39 0.07249137223681146 1107 40-41 0.22158057285185606 1107 42-43 0.19493228563308662 1107 44-45 0.17829294009916907 1107 46-47 0.06782072994899835 1107 48-49 -0.014963423106664209 1107 50-51 0.015193110542170984 1107 52-53 0.10240412328513315 1107 54-55 -0.0866286476003637 1107 56-57 -0.20376448601801656 1107 58-59 -0.08926863892593673 1107 60-61 -0.03206659534724565 1107 62-63 0.031766011450457654 1107 64-65 -0.10671606822544533 1107 66-67 0.026428321347072625 1107 68-69 0.10777795539407009 1107 70-71 -0.016553239336197123 1107 72-73 -0.1273607727468722 1107 74-75 -0.28207960192828807 1107 76-77 -0.3384119722917873 1107 78-79 -0.23120159284452413 1107 80-81 -0.34213990599133126 1107 82-83 -0.3619778529293498 1107 84-85 -0.33103833295140106 1107 86-87 -0.5265354431532181 1107 88-89 -0.6526067230445491 1107 90-91 -0.5518937696014383 1107 92-93 -0.8436177145514954 1107 94-95 -1.065059954555256 1107 96-97 -1.1582254271964523 1107 98-99 -1.1562297063980296 1107 100-101 -0.999164874699094 1108 1 -0.07645351766184305 1108 2 -0.02097796267280927 1108 3 -0.09896996019295301 1108 4 0.023495295398802796 1108 5 0.046737989686782555 1108 6 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96-97 -0.24485407104943135 2316 98-99 -0.09187820660926604 2316 100-101 -0.16739210163354556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8029.0 3 2305.0 4 2092.0 5 2281.0 6 2893.0 7 3434.0 8 4303.0 9 5495.0 10 7162.0 11 9080.0 12 11466.0 13 14311.0 14 16818.0 15 20816.0 16 24454.0 17 30472.0 18 29456.0 19 26016.0 20 26308.0 21 28372.0 22 30466.0 23 33891.0 24 37922.0 25 42403.0 26 48103.0 27 54508.0 28 63096.0 29 72956.0 30 85380.0 31 102014.0 32 123472.0 33 153331.0 34 196755.0 35 259024.0 36 325109.0 37 349318.0 38 182459.0 39 11585.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48516927478683 18.465302494743792 14.86207012257251 23.187458107896873 2 15.355812439903453 19.36114508859948 40.864139286625374 24.418903184871695 3 19.318383638981167 23.46478359118405 29.73025729417571 27.48657547565907 4 12.771278748029527 16.52418715078199 37.08092304633274 33.62361105485574 5 13.745179435533394 36.977557253158444 34.41682428172566 14.860439029582503 6 32.46330054555318 36.086715319501735 17.11235529534591 14.337628839599176 7 28.64167153701712 32.858576482510124 20.127275763335348 18.37247621713741 8 26.349501399222454 34.99037472338574 19.39791727659463 19.26220660079717 9 26.04499608293941 17.07151141265773 20.204218970846025 36.67927353355683 10-11 25.012562235985975 26.472747054751306 26.990125070173665 21.524565639089058 12-13 25.98967773194923 23.799940171440333 27.452428724419914 22.757953372190524 14-15 22.576740307574973 25.327083798754675 26.052506892327482 26.043669001342867 16-17 22.04817854920993 28.448976894746004 26.909669976671964 22.593174579372103 18-19 22.06877885928015 26.780859624247082 29.423981503056673 21.726380013416094 20-21 22.45415084079807 26.002220906553763 29.878394318930855 21.665233933717314 22-23 22.256364046509667 25.909332269980744 29.714904963544832 22.11939871996476 24-25 22.023299252629556 26.014973615896675 29.844553581135546 22.117173550338222 26-27 21.635461739351765 26.480805225437425 29.560984033476583 22.32274900173423 28-29 21.852790870934367 26.86457982593341 29.380804725564662 21.90182457756756 30-31 21.96797803441469 26.40669994525373 29.28663587759986 22.338686142731724 32-33 21.61414811781316 26.36366648924789 29.38347452513203 22.638710867806918 34-35 22.4037383223438 26.563544199841804 29.124571481856844 21.908145995957554 36-37 22.47641719702243 26.103502597318677 29.122072751096635 22.29800745456226 38-39 21.984690475577246 26.46070679407161 29.06148216375811 22.49312056659304 40-41 22.523776698957676 26.3480004350169 29.026645602690564 22.101577263334864 42-43 22.13653535062167 26.33914215500381 28.981108978422732 22.543213515951788 44-45 22.162348319636028 26.62901185237569 29.061027778175198 22.14761204981309 46-47 22.586747061587843 26.918228529063608 28.237230700486876 22.257793708861666 48-49 22.2679104268645 26.92164854433742 28.267379419010368 22.543061609787713 50-51 22.47397468102925 27.19169806276003 28.124637806177066 22.209689450033657 52-53 22.806951575172526 26.843133836888818 28.103500739883643 22.24641384805502 54-55 22.30411941350446 27.09595339661668 28.25948447899575 22.34044271088311 56-57 22.382979714760467 27.225111738128454 28.117729307116313 22.27417923999476 58-59 22.4722116724671 26.80352776543377 27.99369685631833 22.7305637057808 60-61 22.096660037250356 27.098275663679104 28.276405579664488 22.528658719406057 62-63 22.172775639657672 27.046851403514367 28.464204271824972 22.31616868500299 64-65 21.954656185216077 27.11225094819698 28.2394388219037 22.69365404468324 66-67 21.84425649030075 27.097913853434875 28.226408554118592 22.831421102145786 68-69 22.398953446224795 26.770306673865573 28.31351964220258 22.51722023770705 70-71 22.20616684545847 26.883916423553423 27.94182697968315 22.968089751304962 72-73 22.243696534243636 26.977484337152376 27.93225882280797 22.84656030579602 74-75 22.170965173185692 27.300578511991286 27.844241614601135 22.68421470022189 76-77 22.335598173142746 26.883124048511846 27.920734012444697 22.860543765900708 78-79 22.383374012381907 27.100215955886803 27.852015452044228 22.66439457968707 80-81 22.563510874003303 27.142195612306665 27.520826972743368 22.773466540946668 82-83 22.48338234981584 27.15571710923183 27.65631053835301 22.704590002599318 84-85 22.48833648467748 27.234295506509522 27.60250519087991 22.674862817933093 86-87 22.662336030172227 27.171208705740074 27.368449661970445 22.798005602117254 88-89 22.57844056409252 27.248464593344668 27.2126271941463 22.96046764841651 90-91 22.516546674599553 27.226415121352503 27.2526353702158 23.00440283383214 92-93 22.569983867570954 26.80532922218792 27.48743040461931 23.137256505621814 94-95 22.435054698850657 26.747192065691785 27.519821253978254 23.29793198147931 96-97 22.73788979772664 26.416137382085235 27.5560882872646 23.289884532923523 98-99 22.482085934366676 26.560691550186572 27.209040291776354 23.7481822236704 100-101 23.33277485205872 25.42349962369 27.341749830256468 23.90197569399481 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 617.0 1 440.0 2 379.5 3 498.5 4 418.0 5 402.0 6 717.5 7 1632.5 8 2814.0 9 3326.0 10 2785.0 11 2158.5 12 2082.5 13 2174.0 14 2355.0 15 2612.0 16 2875.0 17 3204.5 18 3513.5 19 3897.5 20 4445.5 21 5217.5 22 6382.5 23 7833.0 24 9855.0 25 12552.5 26 15952.5 27 20030.5 28 23441.5 29 26618.0 30 30124.5 31 35285.0 32 42257.5 33 45232.5 34 46274.5 35 49660.0 36 53764.0 37 58127.5 38 61587.0 39 64495.5 40 68547.5 41 73420.0 42 76737.0 43 79378.5 44 82388.0 45 85913.0 46 87914.5 47 89976.0 48 90802.5 49 88424.0 50 87267.0 51 85908.0 52 84006.5 53 83118.5 54 83483.5 55 79565.5 56 72659.5 57 66957.0 58 62456.0 59 55789.0 60 45116.0 61 36999.5 62 31195.0 63 23846.0 64 17416.0 65 13319.0 66 9771.0 67 7179.0 68 5449.5 69 4146.0 70 3069.0 71 2163.5 72 1536.5 73 1132.0 74 805.0 75 564.0 76 409.5 77 267.0 78 168.5 79 116.0 80 76.5 81 46.5 82 31.0 83 24.0 84 20.0 85 13.5 86 6.5 87 4.0 88 6.5 89 7.0 90 3.5 91 1.0 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1461577907577773 2 0.05275082691313683 3 0.15089760169652544 4 0.12846522061572596 5 0.19183976170191902 6 0.19939894294043978 7 0.16340089606943006 8 0.06991221134653533 9 0.11931248225124674 10-11 0.10327475989384458 12-13 0.15142878740517823 14-15 0.13618784361075528 16-17 0.13485987933912327 18-19 0.1338383683609448 20-21 0.1351663326325768 22-23 0.19474085287994589 24-25 0.21490547958918915 26-27 0.14779220832286283 28-29 0.12740284919842032 30-31 0.09967904125065633 32-33 0.12437917670301203 34-35 0.09401987043154753 36-37 0.08748220017120524 38-39 0.05295512910877253 40-41 0.06018742683427619 42-43 0.0638852965752823 44-45 0.041269043518410695 46-47 0.051627164837140506 48-49 0.043455077011712646 50-51 0.05407879118476887 52-53 0.028050691460781128 54-55 0.05174974615452193 56-57 0.02756036619125546 58-59 0.05205619944797546 60-61 0.038224940803438814 62-63 0.05234222252186545 64-65 0.04092172978583001 66-67 0.03900128914685446 68-69 0.06592831853163926 70-71 0.05340459393917106 72-73 0.0523013620827383 74-75 0.044088413818183306 76-77 0.02165603273738383 78-79 0.02284098547207087 80-81 0.026477564554386265 82-83 0.02284098547207087 84-85 0.021063556370040308 86-87 0.024577554134974287 88-89 0.06480465645564293 90-91 0.10948554664116975 92-93 0.08049506508046443 94-95 0.07657246292425905 96-97 0.06862510751403045 98-99 0.05606052248243512 100-101 0.13183620684371494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2447355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.05020982697049 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92023043707282 43.88315286373926 2 13.271827838390596 15.143211259352233 3 4.35964012396059 7.461551515260939 4 1.915909517223412 4.3721215986834165 5 0.9940477201568241 2.8355315506484233 6 0.5899847370539004 2.0195251826181018 7 0.3890381887789513 1.5536297210380607 8 0.2776931444636131 1.2673961727328287 9 0.19829034142735077 1.0181255026582783 >10 0.9788180973570343 10.067246166888092 >50 0.06488549135557618 2.536945938037847 >100 0.034462650607875775 3.6650455784569687 >500 0.0025140267403081533 0.9965631109443074 >1k 0.0025140267403053044 2.417912323619738 >5k 7.18293354372944E-5 0.34635270421046077 >10k+ 7.18293354372944E-5 0.41568881111108774 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10144 0.41448829450570107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8452 0.3453524315025813 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4953 0.20238175499672095 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2911 0.11894473829910249 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2786 0.11383718340821009 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2729 0.11150813837796315 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2512 0.10264142308737392 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.08604391271393E-5 2 0.0 0.0 0.0 0.0 4.08604391271393E-5 3 0.0 0.0 0.0 0.0 4.08604391271393E-5 4 0.0 0.0 0.0 0.0 4.08604391271393E-5 5 0.0 0.0 0.0 0.0 8.17208782542786E-5 6 4.08604391271393E-5 0.0 0.0 0.0 8.17208782542786E-5 7 8.17208782542786E-5 0.0 0.0 4.08604391271393E-5 8.17208782542786E-5 8 8.17208782542786E-5 0.0 0.0 4.08604391271393E-5 8.17208782542786E-5 9 8.17208782542786E-5 0.0 0.0 4.08604391271393E-5 8.17208782542786E-5 10-11 8.17208782542786E-5 0.0 0.0 8.17208782542786E-5 8.17208782542786E-5 12-13 1.225813173814179E-4 0.0 0.0 8.17208782542786E-5 2.860230738899751E-4 14-15 2.043021956356965E-4 0.0 0.0 8.17208782542786E-5 2.860230738899751E-4 16-17 2.043021956356965E-4 0.0 0.0 8.17208782542786E-5 3.064532934535447E-4 18-19 2.8602307388997513E-4 0.0 0.0 1.0215109781784825E-4 3.268835130171144E-4 20-21 3.677439521442537E-4 0.0 0.0 1.225813173814179E-4 3.268835130171144E-4 22-23 3.677439521442537E-4 0.0 0.0 2.043021956356965E-4 3.677439521442537E-4 24-25 4.08604391271393E-4 0.0 0.0 4.6989504996210196E-4 4.08604391271393E-4 26-27 4.08604391271393E-4 0.0 0.0 0.0013075340520684576 4.08604391271393E-4 28-29 6.129065869070894E-4 0.0 0.0 0.003922602156205373 4.08604391271393E-4 30-31 6.94627465161368E-4 0.0 0.0 0.010950597686073333 4.08604391271393E-4 32-33 6.94627465161368E-4 0.0 0.0 0.026967889823911937 5.311857086528109E-4 34-35 6.94627465161368E-4 0.0 0.0 0.05389491920869674 5.720461477799502E-4 36-37 6.94627465161368E-4 0.0 0.0 0.09422417262718322 5.720461477799502E-4 38-39 6.94627465161368E-4 0.0 0.0 0.15616859834392638 5.720461477799502E-4 40-41 7.150576847249377E-4 0.0 0.0 0.21929797679535662 5.720461477799502E-4 42-43 7.763483434156467E-4 0.0 0.0 0.28512414422917803 5.720461477799502E-4 44-45 9.806505390513433E-4 0.0 0.0 0.36874503290286864 5.720461477799502E-4 46-47 0.0010215109781784824 0.0 0.0 0.4640928676060482 5.720461477799502E-4 48-49 0.0010623714173056218 0.0 0.0 0.5623622237068182 5.924763673435198E-4 50-51 0.0010623714173056218 0.0 0.0 0.6664746226027691 6.537670260342288E-4 52-53 0.0010623714173056218 0.0 0.0 0.765418175949137 7.354879042885074E-4 54-55 0.0011032318564327612 0.0 0.0 0.8713897248253727 7.354879042885074E-4 56-57 0.0011032318564327612 0.0 0.0 0.9907022070766195 7.55918123852077E-4 58-59 0.0011032318564327612 0.0 0.0 1.1217824957964824 7.763483434156467E-4 60-61 0.0011032318564327612 0.0 0.0 1.2590940014832341 7.763483434156467E-4 62-63 0.0011032318564327612 0.0 0.0 1.3947506593853365 8.580692216699253E-4 64-65 0.0011032318564327612 0.0 0.0 1.5369245573282175 8.580692216699253E-4 66-67 0.0011440922955599003 0.0 0.0 1.7020824522801146 8.784994412334949E-4 68-69 0.0011440922955599003 0.0 0.0 1.8784156773332843 8.989296607970646E-4 70-71 0.0011440922955599003 0.0 0.0 2.0578951561992436 9.193598803606342E-4 72-73 0.0011440922955599003 0.0 0.0 2.23998970316934 9.602203194877736E-4 74-75 0.0011440922955599003 0.0 0.0 2.428928373693232 9.806505390513433E-4 76-77 0.0011440922955599003 0.0 0.0 2.6317391633007876 9.806505390513433E-4 78-79 0.0011440922955599003 0.0 0.0 2.8588823444085554 0.0010010807586149128 80-81 0.0013075340520684576 0.0 0.0 3.0913782430419783 0.0010623714173056218 82-83 0.0013075340520684576 4.08604391271393E-5 0.0 3.329165568542365 0.0011032318564327612 84-85 0.0013279642716320273 4.08604391271393E-5 0.0 3.570099147855542 0.0011032318564327612 86-87 0.001348394491195597 4.08604391271393E-5 0.0 3.8215951506830845 0.0011440922955599005 88-89 0.001348394491195597 4.08604391271393E-5 0.0 4.08776005115727 0.0012053829542506094 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4635 0.0 57.43307 1 GTATCAA 8300 0.0 44.17867 1 ATCAACG 10435 0.0 34.642143 3 TATCAAC 10545 0.0 34.280773 2 TCAACGC 10750 0.0 33.5828 4 CAACGCA 10890 0.0 33.063713 5 AACGCAG 11275 0.0 31.926859 6 TGGTATC 1785 0.0 28.512096 2 ACGCAGA 12665 0.0 28.150793 7 CGCAGAG 12635 0.0 28.06484 8 GTGGTAT 2055 0.0 26.417578 1 GCAGAGT 13960 0.0 24.822247 9 GAGTACT 9175 0.0 22.613989 12-13 CAGAGTA 13260 0.0 22.33213 10-11 GTACTTT 9760 0.0 21.137648 14-15 AGAGTAC 12730 0.0 21.11494 10-11 TAAGGTG 1520 0.0 20.965963 5 GTACATG 7600 0.0 20.677608 1 ACCGAGT 765 0.0 20.50461 8 TACATGG 7285 0.0 20.109661 2 >>END_MODULE