FastQCFastQC Report
Thu 26 May 2016
SRR938224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447355
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97400.3979806770983368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.3390599238770019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.21149363292207302No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30440.12437917670301203No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27790.11355116033432013No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27660.11301997462566732No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26690.1090565120303348No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25930.10595111865667221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.056.767331
GTATCAA85100.044.0452461
ATCAACG107600.034.738223
TATCAAC108600.034.4677162
TCAACGC109800.034.1698654
CAACGCA111150.033.712125
AACGCAG116000.032.2616
ACGCAGA130200.028.5613127
CGCAGAG131200.028.0178288
TGGTATC20450.027.8743062
GTGGTAT23200.025.3913251
GCAGAGT146300.024.9312639
GAGTACT100400.023.25111812-13
GTGTAAG11350.023.0206971
GTACATG74000.022.8544041
GGACCGA7700.022.8202636
GTGTAGC15850.022.7790681
TACATGG72300.022.6671492
CAGAGTA138600.022.39355710-11
GTACTTT104700.022.22633714-15
Produced by FastQC (version 0.11.5)