FastQCFastQC Report
Thu 26 May 2016
SRR938223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466329
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102350.41498924109476065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79920.3240443590453666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.20597414213594373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59800.045.7879331
GTATCAA87100.037.7895131
ATCAACG109350.029.6836743
TATCAAC110800.029.3809992
TCAACGC111050.029.3148544
CAACGCA112150.028.9425755
AACGCAG115400.028.3293536
TACCGTA1056.902116E-527.1468287
TACATGG80200.026.3694672
GTACATG84700.025.5689451
ACATGGG84600.024.1553763
ACGCAGA134550.024.1153817
CGCAGAG134400.024.0716028
GTACCGT2653.092282E-1123.3104976
TGACGCG1750.023.08932394-95
GAGTACT88000.023.05026212-13
GTACTTT91150.022.01960614-15
GCAGAGT145150.021.894749
CATGGGG58900.021.4625874
GTGGTAT29150.021.1101591
Produced by FastQC (version 0.11.5)