##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2466329 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91005133540578 33.0 31.0 34.0 30.0 34.0 2 32.071335981533686 34.0 31.0 34.0 30.0 34.0 3 32.16230194755039 34.0 31.0 34.0 30.0 34.0 4 35.68621785657955 37.0 35.0 37.0 35.0 37.0 5 35.68602404626471 37.0 35.0 37.0 35.0 37.0 6 35.72069419773274 37.0 35.0 37.0 35.0 37.0 7 35.65300047155104 37.0 35.0 37.0 33.0 37.0 8 35.62242223158386 37.0 35.0 37.0 33.0 37.0 9 37.2555510639497 39.0 37.0 39.0 34.0 39.0 10-11 37.148754890365396 39.0 37.0 39.0 34.0 39.0 12-13 37.00881755840361 39.0 37.0 39.0 33.0 39.0 14-15 38.182394157470476 40.0 38.0 41.0 33.0 41.0 16-17 37.99409425911953 40.0 38.0 41.0 33.0 41.0 18-19 38.16328539298691 40.0 38.0 41.0 33.0 41.0 20-21 38.14664426359987 40.0 38.0 41.0 33.0 41.0 22-23 38.03308561023286 40.0 38.0 41.0 33.0 41.0 24-25 37.89328147217991 40.0 38.0 41.0 33.0 41.0 26-27 37.68139206083211 40.0 38.0 41.0 32.0 41.0 28-29 37.46775430204162 40.0 37.0 41.0 32.0 41.0 30-31 37.22229414648248 40.0 37.0 41.0 31.0 41.0 32-33 36.90552558073152 39.5 36.0 41.0 30.5 41.0 34-35 36.60479299395985 39.0 36.0 41.0 30.0 41.0 36-37 36.2985072145687 39.0 35.0 40.5 30.0 41.0 38-39 35.96779525359349 39.0 35.0 40.0 29.0 41.0 40-41 35.814692200432305 39.0 35.0 40.0 28.5 41.0 42-43 35.53960907080929 38.5 35.0 40.0 27.0 41.0 44-45 35.172191950060196 38.0 34.5 40.0 25.5 41.0 46-47 34.70253015716881 38.0 34.0 40.0 24.0 41.0 48-49 34.219359217687504 38.0 33.0 40.0 22.5 41.0 50-51 33.386166646866656 37.5 32.5 39.5 20.5 40.5 52-53 33.36308254089377 37.5 33.0 39.5 20.0 40.5 54-55 33.33071094732293 38.0 33.0 40.0 18.0 41.0 56-57 32.83319885546494 37.0 32.0 40.0 12.5 41.0 58-59 32.35606603985113 36.5 31.0 40.0 8.0 41.0 60-61 31.76600303528037 36.0 31.0 39.0 4.5 41.0 62-63 31.12408624315734 35.5 30.0 39.0 2.0 40.5 64-65 30.48395428995888 35.0 29.0 38.5 2.0 40.0 66-67 29.775926488315225 35.0 28.0 38.0 2.0 40.0 68-69 29.053391295321916 34.0 27.0 37.5 2.0 40.0 70-71 28.217098570385378 34.0 26.0 36.5 2.0 39.0 72-73 27.40481460502634 33.5 24.5 36.0 2.0 39.0 74-75 26.558197831676146 33.0 21.5 35.5 2.0 38.5 76-77 25.71242036240907 32.0 19.0 35.0 2.0 37.0 78-79 24.880433632333723 31.5 11.5 35.0 2.0 37.0 80-81 24.082000211650595 31.0 4.5 35.0 2.0 36.0 82-83 23.26114318081651 30.5 2.0 34.5 2.0 36.0 84-85 22.44846003919185 30.0 2.0 34.0 2.0 35.0 86-87 21.67408930438721 29.0 2.0 34.0 2.0 35.0 88-89 20.837845437490294 29.0 2.0 34.0 2.0 35.0 90-91 19.974240865675263 27.0 2.0 34.0 2.0 35.0 92-93 19.048264039388094 25.5 2.0 33.5 2.0 35.0 94-95 18.101066808199555 24.5 2.0 33.0 2.0 35.0 96-97 16.971909668174845 18.5 2.0 32.5 2.0 35.0 98-99 15.658370801300231 2.0 2.0 32.0 2.0 34.0 100-101 12.668008607124191 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5466721668829884 1101 2 0.5850474402511097 1101 3 0.5528422079160364 1101 4 0.45243995643534873 1101 5 0.4569393567709952 1101 6 0.42711899023068156 1101 7 0.44689250397654234 1101 8 0.4256583370748146 1101 9 0.505576687871546 1101 10-11 0.5928988125021419 1101 12-13 0.6714154609056138 1101 14-15 0.9109964329669467 1101 16-17 0.8942341231709463 1101 18-19 0.8396203252676955 1101 20-21 0.7327151192270165 1101 22-23 0.9486367827902313 1101 24-25 0.9483935301739734 1101 26-27 1.0789857866169825 1101 28-29 1.081988479850395 1101 30-31 1.0282276401387236 1101 32-33 1.3130139609786582 1101 34-35 1.369689386382511 1101 36-37 1.2999400920131663 1101 38-39 1.5567876831804242 1101 40-41 1.633478104390285 1101 42-43 1.8156630088493273 1101 44-45 1.9005437727701633 1101 46-47 1.9056190783102167 1101 48-49 2.096006303289279 1101 50-51 2.361481048307759 1101 52-53 2.4441799280674203 1101 54-55 2.4524370026691145 1101 56-57 2.649625966072186 1101 58-59 2.900698541311634 1101 60-61 2.9551154260147037 1101 62-63 3.08298761050683 1101 64-65 3.069261120289976 1101 66-67 3.3808915903219408 1101 68-69 3.4278472512641045 1101 70-71 3.949193670754095 1101 72-73 4.133215819651284 1101 74-75 4.331234339043327 1101 76-77 4.510640408234128 1101 78-79 4.694260673662093 1101 80-81 4.848024494501622 1101 82-83 5.061902559609933 1101 84-85 5.3061870932465744 1101 86-87 5.4429551880805285 1101 88-89 5.537811211548853 1101 90-91 5.618702000598066 1101 92-93 5.8490137852687845 1101 94-95 5.815763572665123 1101 96-97 5.626730281568992 1101 98-99 5.447743397986711 1101 100-101 4.719395365878626 1102 1 0.47840367199046696 1102 2 0.3710437261879491 1102 3 0.3675278846544785 1102 4 0.345911199291443 1102 5 0.3668797169959177 1102 6 0.30679800245010114 1102 7 0.3413906963749014 1102 8 0.36944392875967935 1102 9 0.44858333193680267 1102 10-11 0.5509489890010997 1102 12-13 0.5365354392927983 1102 14-15 0.7629932958704657 1102 16-17 0.7259749390035282 1102 18-19 0.7684506979182757 1102 20-21 0.6431915385905924 1102 22-23 0.7849411322837412 1102 24-25 0.6782762917393015 1102 26-27 0.8322398530814965 1102 28-29 0.8371337740352232 1102 30-31 0.8415860591648112 1102 32-33 0.945883760821502 1102 34-35 1.0006550683707616 1102 36-37 1.1699027127543147 1102 38-39 1.1847928954820048 1102 40-41 1.3431410390967429 1102 42-43 1.4020898678836673 1102 44-45 1.5597447080186484 1102 46-47 1.7279688453929438 1102 48-49 1.9284106947130155 1102 50-51 2.074776550694885 1102 52-53 2.085548268022663 1102 54-55 2.176889289144576 1102 56-57 2.490983141679706 1102 58-59 2.5397903344081456 1102 60-61 2.809152232568092 1102 62-63 2.7562923767458436 1102 64-65 2.8399776967043984 1102 66-67 2.9270927079498428 1102 68-69 3.1711590825750733 1102 70-71 3.3432742654132745 1102 72-73 3.7552302088762417 1102 74-75 3.7026334107832106 1102 76-77 3.969509374585975 1102 78-79 4.329375913593303 1102 80-81 4.380627798807652 1102 82-83 4.591028617027067 1102 84-85 4.797580318618106 1102 86-87 4.944321009665732 1102 88-89 4.979677345053997 1102 90-91 5.077602925960566 1102 92-93 5.281559073317478 1102 94-95 5.4187622440225205 1102 96-97 5.250185809114246 1102 98-99 5.077132328919456 1102 100-101 4.291663637484195 1103 1 0.42332649163050107 1103 2 0.36554982863591334 1103 3 0.3361806998074428 1103 4 0.21599068850922265 1103 5 0.31877055109580965 1103 6 0.2324052157220109 1103 7 0.3173081458280649 1103 8 0.30268507109514786 1103 9 0.2641292737160228 1103 10-11 0.38929852516769614 1103 12-13 0.43437654496418077 1103 14-15 0.6279689028183455 1103 16-17 0.6512199004470318 1103 18-19 0.669390184612844 1103 20-21 0.6135747722408809 1103 22-23 0.6796850817901827 1103 24-25 0.6761575931756951 1103 26-27 0.7270594375850763 1103 28-29 0.7266572755946754 1103 30-31 0.6532754326620633 1103 32-33 0.7846289262039079 1103 34-35 0.8495739064048919 1103 36-37 0.9518963165445129 1103 38-39 1.2532344021719766 1103 40-41 1.3681226356014875 1103 42-43 1.261270772506812 1103 44-45 1.272306915507194 1103 46-47 1.4869547117535618 1103 48-49 1.5964523668405022 1103 50-51 1.7665162699492072 1103 52-53 1.8240274554353633 1103 54-55 1.9796172934462746 1103 56-57 2.1750044166509923 1103 58-59 2.5319243967771143 1103 60-61 2.6587264718392554 1103 62-63 2.755008419081843 1103 64-65 2.7447637486718612 1103 66-67 3.0407447534835406 1103 68-69 3.118367283572681 1103 70-71 3.2766730061773295 1103 72-73 3.567046967631832 1103 74-75 3.785843441719045 1103 76-77 3.9734181014152306 1103 78-79 3.9236188552455467 1103 80-81 4.109310978196714 1103 82-83 4.408261596907135 1103 84-85 4.386974250588661 1103 86-87 4.380330637868173 1103 88-89 4.746477092569883 1103 90-91 5.02524258327789 1103 92-93 5.202743629651362 1103 94-95 5.1726535166216685 1103 96-97 4.899937999875302 1103 98-99 4.948104841875393 1103 100-101 4.208372051402993 1104 1 0.5443523787288633 1104 2 0.415792088818435 1104 3 0.40119965181900596 1104 4 0.3088907310028688 1104 5 0.27629894431399293 1104 6 0.3064004188505365 1104 7 0.34479155919303395 1104 8 0.33822893756843797 1104 9 0.3648355420894944 1104 10-11 0.5060499149109887 1104 12-13 0.4686189226905455 1104 14-15 0.673846671140204 1104 16-17 0.7725043646694303 1104 18-19 0.6415074508368974 1104 20-21 0.6171328901319484 1104 22-23 0.6194551714444287 1104 24-25 0.6994456772845012 1104 26-27 0.7581211716893037 1104 28-29 0.8307682555430063 1104 30-31 0.8276218560232849 1104 32-33 0.9352704487988106 1104 34-35 1.0510204690014362 1104 36-37 1.143706990530866 1104 38-39 1.2183697960540485 1104 40-41 1.311521794776347 1104 42-43 1.3218325315504416 1104 44-45 1.4585161806687026 1104 46-47 1.4978491701413006 1104 48-49 1.8280061031301145 1104 50-51 1.983657835656409 1104 52-53 1.9937326369149417 1104 54-55 2.057473850517489 1104 56-57 2.351319409406962 1104 58-59 2.434443720946412 1104 60-61 2.60377153482381 1104 62-63 2.7289012160028143 1104 64-65 2.8155195362562218 1104 66-67 2.9556809994811495 1104 68-69 2.9991654526726492 1104 70-71 3.1108112957365357 1104 72-73 3.371242992871281 1104 74-75 3.6744931722768506 1104 76-77 3.7995106308466227 1104 78-79 3.8925423314964718 1104 80-81 4.216917824197651 1104 82-83 4.490842527197064 1104 84-85 4.519050708992641 1104 86-87 4.792698512207618 1104 88-89 4.9047271624582045 1104 90-91 4.925023599869746 1104 92-93 5.1137520166366315 1104 94-95 5.164583783736187 1104 96-97 5.272974795351722 1104 98-99 4.978524628061594 1104 100-101 3.9968248380234144 1105 1 0.3145380084509206 1105 2 0.2961375214234607 1105 3 0.3062339523488049 1105 4 0.17051434959432754 1105 5 0.22220464626074232 1105 6 0.21842375893084665 1105 7 0.1510189604808687 1105 8 0.20908416484078884 1105 9 0.3236729002967422 1105 10-11 0.3200447900286889 1105 12-13 0.3460060861934764 1105 14-15 0.5693257524400437 1105 16-17 0.6275194528175092 1105 18-19 0.45839685772960337 1105 20-21 0.5179768489366623 1105 22-23 0.5674273670157177 1105 24-25 0.5708604070446199 1105 26-27 0.5401871109176355 1105 28-29 0.6850900748816997 1105 30-31 0.8626720060841109 1105 32-33 0.8323363068737493 1105 34-35 0.9634007928235278 1105 36-37 0.9983783165572291 1105 38-39 1.0108286581230104 1105 40-41 1.1102184348253061 1105 42-43 1.153594163794999 1105 44-45 1.2993366286974393 1105 46-47 1.6537366298997043 1105 48-49 1.7345317112197804 1105 50-51 1.7421560091579877 1105 52-53 1.7182646508339374 1105 54-55 1.847489357198235 1105 56-57 2.2126247060973867 1105 58-59 2.3942575641832207 1105 60-61 2.428631754944636 1105 62-63 2.494157825764237 1105 64-65 2.6735149599343693 1105 66-67 2.939967058440825 1105 68-69 2.996868538124616 1105 70-71 3.321113016753788 1105 72-73 3.4776518677332966 1105 74-75 3.5714535309420157 1105 76-77 3.8952244916453935 1105 78-79 4.121763917277519 1105 80-81 3.996219077078372 1105 82-83 4.0364203694396075 1105 84-85 4.090852403657838 1105 86-87 4.331152819109324 1105 88-89 4.60603954958432 1105 90-91 4.9012855032031055 1105 92-93 4.902943770795467 1105 94-95 4.710578608520539 1105 96-97 4.65488767381148 1105 98-99 4.318019175556659 1105 100-101 3.6863292242274817 1106 1 0.4400912531999772 1106 2 0.33078037507802094 1106 3 0.31327212553883044 1106 4 0.32357582324857503 1106 5 0.26714758461127985 1106 6 0.26224416159256236 1106 7 0.1614437519850398 1106 8 0.18556027485347215 1106 9 0.37730537415889387 1106 10-11 0.31667735737734404 1106 12-13 0.3958416301802714 1106 14-15 0.6767416564475397 1106 16-17 0.5652494191484223 1106 18-19 0.49789926535587625 1106 20-21 0.6099026848385449 1106 22-23 0.6823666670669297 1106 24-25 0.6554951678377705 1106 26-27 0.7667057782187072 1106 28-29 0.8330668246490234 1106 30-31 0.8614099383022165 1106 32-33 0.7022408564571734 1106 34-35 0.6825166721338505 1106 36-37 0.8599977992838532 1106 38-39 0.9134496464009132 1106 40-41 0.991385142886493 1106 42-43 0.9171455206748433 1106 44-45 1.0344453276862495 1106 46-47 1.3385864794143387 1106 48-49 1.403728240079893 1106 50-51 1.6486997678176962 1106 52-53 1.559664712367585 1106 54-55 1.5754578501539527 1106 56-57 1.524418324876173 1106 58-59 1.9206529374385468 1106 60-61 2.2879928235372127 1106 62-63 2.0686287200672844 1106 64-65 2.394775360307758 1106 66-67 2.5795146744652477 1106 68-69 2.406263373483725 1106 70-71 2.6115459290257768 1106 72-73 2.9709839778352816 1106 74-75 3.072033286788759 1106 76-77 2.8999900358693935 1106 78-79 3.3852218748625518 1106 80-81 3.484632332069083 1106 82-83 3.3406018148804684 1106 84-85 3.525936318258612 1106 86-87 3.630492239297663 1106 88-89 3.822698791980873 1106 90-91 3.8902881940795204 1106 92-93 3.919484428354412 1106 94-95 3.821640050082408 1106 96-97 3.933261176108875 1106 98-99 3.8126477132184657 1106 100-101 3.3671424974969 1107 1 0.5249408070839117 1107 2 0.4774458935837771 1107 3 0.38009786804708057 1107 4 0.3533969879281784 1107 5 0.43161330089701266 1107 6 0.3879969477346279 1107 7 0.3605001303892479 1107 8 0.31016027040897853 1107 9 0.40207648272676266 1107 10-11 0.46705958384652035 1107 12-13 0.44275673479254607 1107 14-15 0.6297390508630301 1107 16-17 0.617859031477451 1107 18-19 0.5505456836494105 1107 20-21 0.5821093204678291 1107 22-23 0.5580986196441771 1107 24-25 0.6039383449841864 1107 26-27 0.6937549676541437 1107 28-29 0.7681154085221777 1107 30-31 0.785649374295943 1107 32-33 0.8303471409714476 1107 34-35 0.9840241888840424 1107 36-37 1.0094348763624836 1107 38-39 1.0064409574154851 1107 40-41 0.984194090763701 1107 42-43 1.0025110525140803 1107 44-45 1.1670447176794667 1107 46-47 1.3472025473706495 1107 48-49 1.2722295567611965 1107 50-51 1.3489089368084635 1107 52-53 1.413630450875793 1107 54-55 1.5853412933033226 1107 56-57 1.6460724543664966 1107 58-59 2.02225614969646 1107 60-61 2.0800509297383947 1107 62-63 2.0190337245673327 1107 64-65 2.2315749358585926 1107 66-67 2.2983969676018354 1107 68-69 2.4400595823304876 1107 70-71 2.584335904469949 1107 72-73 2.929364070947429 1107 74-75 3.0602178777286575 1107 76-77 3.0564521910044498 1107 78-79 3.2544356994606076 1107 80-81 3.4838489900319516 1107 82-83 3.761594687023493 1107 84-85 3.6162921833237824 1107 86-87 3.7080403231652603 1107 88-89 3.870499323097885 1107 90-91 3.821570763885852 1107 92-93 3.9031238299033184 1107 94-95 3.9126688392911753 1107 96-97 3.904005989940142 1107 98-99 3.663092579272531 1107 100-101 2.963493568064351 1108 1 0.4979462693514414 1108 2 0.44753795362242954 1108 3 0.4300900565416015 1108 4 0.30670443721125906 1108 5 0.3049555258452301 1108 6 0.2210694153602759 1108 7 0.27729891758069414 1108 8 0.3413817005509756 1108 9 0.3360056121200117 1108 10-11 0.4262300019645835 1108 12-13 0.39144093009175407 1108 14-15 0.585876001019848 1108 16-17 0.5505665282081864 1108 18-19 0.6356939865275208 1108 20-21 0.5911352257345186 1108 22-23 0.5264432169755366 1108 24-25 0.5069469318539177 1108 26-27 0.7517879437798669 1108 28-29 0.7203147812872146 1108 30-31 0.8011523195228278 1108 32-33 0.8309525326461156 1108 34-35 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2311 96-97 4.753721980311191 2311 98-99 4.731335665864025 2311 100-101 4.380430309019946 2312 1 -0.2771947900698386 2312 2 -0.16004424037679144 2312 3 -0.07273113188402647 2312 4 0.03465124105237294 2312 5 -0.13530352703079984 2312 6 -0.015141963683142023 2312 7 -0.05463606628126172 2312 8 -0.037300788197413226 2312 9 -0.0918092924085201 2312 10-11 -0.1520936168598439 2312 12-13 -0.10697762247351505 2312 14-15 -0.10178758351514006 2312 16-17 0.0088289462517821 2312 18-19 -0.06380911665469569 2312 20-21 -0.029916157785784492 2312 22-23 -0.013390203477825935 2312 24-25 0.1336707048682939 2312 26-27 0.10016493196113885 2312 28-29 0.13890572209604102 2312 30-31 0.14910233096514958 2312 32-33 0.15948350444298143 2312 34-35 0.3065466042838878 2312 36-37 0.2943061132163294 2312 38-39 0.29404590306614864 2312 40-41 0.49648222021097155 2312 42-43 0.6216478601840265 2312 44-45 0.6302099425647327 2312 46-47 0.4965563293423614 2312 48-49 0.5993959607363806 2312 50-51 0.7241601204310157 2312 52-53 0.8058747164858957 2312 54-55 0.9991976999739123 2312 56-57 1.1572154131267567 2312 58-59 1.2026365104460055 2312 60-61 1.2727241792038448 2312 62-63 1.3728980391782564 2312 64-65 1.5743419957969813 2312 66-67 1.7446336250299872 2312 68-69 2.008207021148955 2312 70-71 2.529259381473768 2312 72-73 2.7101911381622017 2312 74-75 2.2717356402257423 2312 76-77 2.686267286618911 2312 78-79 3.0202903341723832 2312 80-81 3.345959530769477 2312 82-83 3.5534933239689543 2312 84-85 3.7427186796680587 2312 86-87 4.063526415222508 2312 88-89 3.9559059096839384 2312 90-91 4.336362042004822 2312 92-93 4.38941585197907 2312 94-95 4.380120125532631 2312 96-97 4.256078024417189 2312 98-99 4.108583769035301 2312 100-101 3.6515578813457026 2313 1 -0.3372210086470879 2313 2 -0.16000287890523168 2313 3 -0.21885776411428992 2313 4 -0.07726600740892309 2313 5 -0.06566421310527204 2313 6 -0.15058878310725987 2313 7 -0.029112336222056 2313 8 -0.1351141578335202 2313 9 -0.23168447012561444 2313 10-11 -0.16263964317774082 2313 12-13 -0.11181806357573265 2313 14-15 -0.042251447312331436 2313 16-17 0.0034393805510717357 2313 18-19 0.016985092535030333 2313 20-21 -0.03826115382006634 2313 22-23 -0.004903761423534547 2313 24-25 0.1136044550248414 2313 26-27 0.10940264642324138 2313 28-29 0.1990899185160515 2313 30-31 0.15421174929137038 2313 32-33 0.17220534841909085 2313 34-35 0.38027961879346606 2313 36-37 0.5035573722837725 2313 38-39 0.6195142802424272 2313 40-41 0.7091200774212112 2313 42-43 0.6902223715346238 2313 44-45 0.9133820023289942 2313 46-47 0.9456737406453044 2313 48-49 1.186169774890864 2313 50-51 1.2835275554465895 2313 52-53 1.246707769714405 2313 54-55 1.6068684401923363 2313 56-57 1.7653094701343548 2313 58-59 1.9309351739726068 2313 60-61 2.018773360802019 2313 62-63 1.938264227988011 2313 64-65 2.217580920720426 2313 66-67 2.338122359199165 2313 68-69 2.648656855237114 2313 70-71 2.96271464604229 2313 72-73 3.3991385104130174 2313 74-75 3.595214581683557 2313 76-77 3.7712720042521894 2313 78-79 3.852052165627704 2313 80-81 4.011402863830046 2313 82-83 4.202138729392438 2313 84-85 4.3292962183209305 2313 86-87 4.562574718400054 2313 88-89 4.685195652792178 2313 90-91 4.674737049361163 2313 92-93 4.744047641052926 2313 94-95 4.707493428260175 2313 96-97 4.721197538006283 2313 98-99 4.790322947425414 2313 100-101 4.385012622512889 2314 1 -0.4629302285921071 2314 2 -0.38593655554159767 2314 3 -0.24999191342514138 2314 4 -0.21903295654693267 2314 5 -0.248875058358756 2314 6 -0.26135839299892893 2314 7 -0.19431214260580276 2314 8 -0.28151146013991735 2314 9 -0.17609671596142107 2314 10-11 -0.2889051668945086 2314 12-13 -0.26277406830052286 2314 14-15 -0.24318746331505992 2314 16-17 -0.17016328284816495 2314 18-19 -0.20968548450704105 2314 20-21 -0.24669183827212038 2314 22-23 -0.19977175502125988 2314 24-25 -0.23389053677145455 2314 26-27 -0.1839441588841524 2314 28-29 -0.14866075010180424 2314 30-31 -0.08934482736562188 2314 32-33 -0.05848189617966426 2314 34-35 0.002427245304190251 2314 36-37 0.0713825846272087 2314 38-39 0.17040260256644757 2314 40-41 0.28138328822726777 2314 42-43 0.31384906549308056 2314 44-45 0.28967667626541527 2314 46-47 0.4966961784757231 2314 48-49 0.4973341008030516 2314 50-51 0.5100781021724359 2314 52-53 0.8522150300787743 2314 54-55 0.9750502283109057 2314 56-57 1.2451530118419498 2314 58-59 1.4000521084865127 2314 60-61 1.3764761961140586 2314 62-63 1.539425900985261 2314 64-65 1.664858094236731 2314 66-67 1.7156184386171027 2314 68-69 1.9715352826538037 2314 70-71 2.3435396070569325 2314 72-73 2.580122609580595 2314 74-75 2.8347094794152774 2314 76-77 3.012842017935643 2314 78-79 3.107831380394277 2314 80-81 3.45299023291739 2314 82-83 3.6234312991239293 2314 84-85 3.7756661831104026 2314 86-87 3.9657984999821103 2314 88-89 4.114019389987515 2314 90-91 4.1944906099747605 2314 92-93 4.368731275433728 2314 94-95 4.295186964897102 2314 96-97 4.249569275325079 2314 98-99 4.283787602906559 2314 100-101 3.906398779147061 2315 1 -0.6430699734100642 2315 2 -0.49468371369822606 2315 3 -0.33044098425555646 2315 4 -0.2731333135348848 2315 5 -0.22640483515787935 2315 6 -0.2455722807359919 2315 7 -0.2603284620552131 2315 8 -0.2646906070864503 2315 9 -0.5662244976315876 2315 10-11 -0.4374586269132834 2315 12-13 -0.38401032378759936 2315 14-15 -0.49282499423574677 2315 16-17 -0.371730407546103 2315 18-19 -0.49898597399519673 2315 20-21 -0.43211133486667563 2315 22-23 -0.3709605868746877 2315 24-25 -0.2238517399495592 2315 26-27 -0.24544701145146774 2315 28-29 -0.15736227194766172 2315 30-31 -0.22754897300146126 2315 32-33 -0.25465297395918896 2315 34-35 -0.24337254593649504 2315 36-37 -0.024423402666123195 2315 38-39 -0.08978227811187622 2315 40-41 -0.007328337950575303 2315 42-43 0.1981041808111499 2315 44-45 0.4234868673421417 2315 46-47 0.5814336326606764 2315 48-49 0.5702126677037427 2315 50-51 0.6920853610864057 2315 52-53 0.7674096576245191 2315 54-55 0.9895927213165194 2315 56-57 1.2577015674467518 2315 58-59 1.4879099901037804 2315 60-61 1.5937982145020406 2315 62-63 1.6104860455915961 2315 64-65 1.7671649322957457 2315 66-67 1.8751041185862185 2315 68-69 2.245096130273801 2315 70-71 2.6369617822527047 2315 72-73 2.813692703617612 2315 74-75 3.0378785220298212 2315 76-77 3.140190798504822 2315 78-79 3.4344045819900018 2315 80-81 3.645900042221953 2315 82-83 3.7878847834502984 2315 84-85 3.9801271026999387 2315 86-87 4.093411089407834 2315 88-89 4.338074372805384 2315 90-91 4.3348068946309475 2315 92-93 4.582039085796385 2315 94-95 4.7462553073577 2315 96-97 4.928109961943036 2315 98-99 4.789288863564964 2315 100-101 4.452648856597719 2316 1 -0.6610912559422104 2316 2 -0.7154474267336894 2316 3 -0.6331544541276699 2316 4 -0.6005798688090778 2316 5 -0.5587863526243169 2316 6 -0.5954583806925484 2316 7 -0.638402085630581 2316 8 -0.4285427948502303 2316 9 -0.5461063947648412 2316 10-11 -0.6228139067542173 2316 12-13 -0.5279849231949711 2316 14-15 -0.6635643379595919 2316 16-17 -0.4872912725507135 2316 18-19 -0.546665415125652 2316 20-21 -0.5065078412256554 2316 22-23 -0.5647358170587324 2316 24-25 -0.614138626787657 2316 26-27 -0.5318842534104746 2316 28-29 -0.5528659573645669 2316 30-31 -0.5261789938743036 2316 32-33 -0.4691009737331626 2316 34-35 -0.4559780138449341 2316 36-37 -0.22969971181026239 2316 38-39 -0.34932003818364166 2316 40-41 -0.23206018722815003 2316 42-43 -0.2213397044341079 2316 44-45 -0.07328260956528254 2316 46-47 0.1330575470784794 2316 48-49 0.15949688098113057 2316 50-51 0.2104488735165262 2316 52-53 0.28868000511678105 2316 54-55 0.5730244148424006 2316 56-57 0.7376698167059672 2316 58-59 0.8631562437635907 2316 60-61 0.8336499855152759 2316 62-63 1.0864121677277545 2316 64-65 1.2597727311818225 2316 66-67 1.4158466055980803 2316 68-69 1.7384295342428224 2316 70-71 2.1227256895363062 2316 72-73 2.633764716699339 2316 74-75 2.598436084816118 2316 76-77 3.017301834271205 2316 78-79 3.434971609958197 2316 80-81 3.4182030834414086 2316 82-83 3.769405781673811 2316 84-85 3.9484577695361303 2316 86-87 4.323367507189662 2316 88-89 4.4579727986891236 2316 90-91 4.774262576035749 2316 92-93 4.856047802240017 2316 94-95 5.022488341548904 2316 96-97 5.079125879694843 2316 98-99 4.758552451222082 2316 100-101 4.713663785449924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6815.0 3 2251.0 4 2439.0 5 2742.0 6 3258.0 7 4154.0 8 5209.0 9 6654.0 10 8559.0 11 10752.0 12 13792.0 13 16850.0 14 19630.0 15 24120.0 16 28756.0 17 34820.0 18 35657.0 19 33736.0 20 36358.0 21 39692.0 22 44192.0 23 49698.0 24 54976.0 25 60475.0 26 66827.0 27 73090.0 28 80095.0 29 88094.0 30 96618.0 31 109707.0 32 124310.0 33 146013.0 34 176647.0 35 222676.0 36 273837.0 37 297014.0 38 155809.0 39 10007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2496045537176 17.894727844221435 14.135403412194645 23.720264189866317 2 15.929763428510245 19.17457103876995 39.82923473997901 25.066430792740796 3 19.776940242230857 22.92175506020074 28.806341471923425 28.494963225644977 4 13.313125241091624 15.6130024592867 35.24949205974489 35.82438023987679 5 14.51744497642209 36.53977775947569 33.48907236956078 15.45370489454144 6 34.234062087076396 34.36867239267587 16.4299626799216 14.967302840326132 7 30.090951051753358 31.83947934335482 19.50410099038654 18.56546861450528 8 27.182934644181085 34.46580520607727 18.71717810819361 19.634082041548034 9 26.649569231430668 16.713195864329702 19.504785483701532 37.132449420538094 10-11 25.97299777431899 25.83235906977687 26.02925325613583 22.165389899768307 12-13 26.62562765154895 23.02603080866666 26.81359864370483 23.53474289607956 14-15 23.42843469558878 24.54578360528085 25.009464848253188 27.016316850877185 16-17 23.06810623144438 27.67263826473499 25.828128295397395 23.431127208423238 18-19 23.11346973373228 26.195852424758915 28.037754635426857 22.652923206081947 20-21 23.51968400455594 25.282251783923755 28.56577884212883 22.632285369391468 22-23 23.41674892910127 25.054710940484714 28.424665590022414 23.1038745403916 24-25 23.295112736967088 25.385136804341137 28.357151230973365 22.96259922771841 26-27 22.78394111642636 25.588362170808526 28.436712266201063 23.190984446564052 28-29 22.934880259254076 26.083324714514294 28.24052386196099 22.741271164270636 30-31 22.99291604902567 25.606285325701727 28.21409760799785 23.18670101727475 32-33 22.786674129867812 25.621331775795703 28.275615207083547 23.316378887252935 34-35 23.42180411772138 25.71281163739667 27.95930423686724 22.906080008014715 36-37 23.488898862294867 25.282895119766387 28.042315273243716 23.185890744695026 38-39 22.952917824726736 25.693654915639293 28.070281091517618 23.28314616811636 40-41 23.36511566843776 25.65344379595639 27.982019410034702 22.999421125571146 42-43 23.072137992113532 25.60665143009153 27.797916136117006 23.523294441677926 44-45 23.076930875043463 25.81471993479524 27.959624139955054 23.14872505020625 46-47 23.45423529967576 26.1509734588279 27.381364425944277 23.013426815552062 48-49 23.339621585629043 26.054187919256243 27.361318489164933 23.24487200594978 50-51 23.386224612895443 26.26072305474511 27.2949415364153 23.058110795944145 52-53 23.48340903616949 26.136475142951443 27.168113819437178 23.21200200144188 54-55 23.309619435529488 26.148819745317198 27.253472440450004 23.28808837870331 56-57 23.192062319984785 26.403231646912893 27.16903253746486 23.235673495637464 58-59 23.347116820936844 25.97401465228794 27.242242834830083 23.436625691945135 60-61 23.241528026759 26.173007358400362 27.325790976339338 23.259673638501297 62-63 23.085140396309352 26.22781512862304 27.47689290256447 23.210151572503136 64-65 23.034656450352596 26.231498778851485 27.34508971782001 23.388755052975906 66-67 23.053983752364463 26.18310318890765 27.218438856461646 23.544474202266237 68-69 23.143277980915734 25.93915457891648 27.495496554877153 23.422070885290637 70-71 23.246872353243063 26.005673856078875 27.172502703405264 23.574951087272797 72-73 23.23728821119897 26.09451333132465 26.99726556686465 23.670932890611727 74-75 23.144506678155967 26.48868388372152 26.881334110550725 23.485475327571788 76-77 23.465024147145527 26.183595349799077 26.814523159768545 23.536857343286847 78-79 23.41543088492333 26.194659979324058 26.880465372869427 23.50944376288318 80-81 23.454082302964835 26.267381639409145 26.74292853354354 23.53560752408248 82-83 23.595766918993004 26.320343950503894 26.516244078339774 23.567645052163325 84-85 23.47773797530742 26.2982438818485 26.58871023168579 23.63530791115829 86-87 23.588449641225587 26.110088216702422 26.65886284506468 23.64259929700731 88-89 23.57645201833296 26.244432160465713 26.38566862538898 23.793447195812345 90-91 23.65566498277753 26.143357208425027 26.40029004556092 23.80068776323652 92-93 23.676644989822567 25.942180348666493 26.476386514087313 23.904788147423627 94-95 23.6035833984939 25.78050731150211 26.609763377923706 24.00614591208028 96-97 23.66037754213162 25.597178896961854 26.492146299603707 24.250297261302826 98-99 23.537818295368368 25.71966486473334 26.22052257418274 24.52199426571555 100-101 24.43454709477609 24.59163284959445 26.365666817866778 24.608153237762682 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1025.0 1 713.5 2 533.0 3 696.0 4 562.5 5 502.5 6 1000.5 7 2213.5 8 3765.0 9 4351.0 10 3574.0 11 2774.0 12 2643.5 13 2767.0 14 2823.5 15 2868.0 16 2956.0 17 3012.5 18 3198.0 19 3515.5 20 3813.0 21 4388.5 22 5369.5 23 6397.0 24 7661.0 25 9711.0 26 12137.0 27 14612.0 28 16653.5 29 18690.5 30 21540.0 31 25270.0 32 30127.0 33 33034.0 34 34760.5 35 37579.0 36 41657.0 37 46998.0 38 51305.5 39 54167.0 40 57153.5 41 62194.5 42 67076.5 43 70558.5 44 74522.0 45 79849.0 46 84956.5 47 90450.0 48 94012.0 49 94491.0 50 94043.5 51 92335.5 52 92331.5 53 95338.5 54 98503.0 55 95158.5 56 87754.5 57 81164.0 58 75377.0 59 68195.0 60 58198.5 61 49197.0 62 41400.0 63 33186.0 64 25472.5 65 19648.5 66 15132.5 67 11776.0 68 9151.0 69 7051.0 70 5488.5 71 3953.0 72 2906.0 73 2204.0 74 1652.0 75 1225.0 76 832.5 77 542.5 78 352.5 79 207.0 80 132.0 81 91.5 82 52.0 83 36.5 84 27.0 85 14.5 86 17.5 87 14.5 88 9.5 89 10.0 90 9.5 91 4.0 92 1.0 93 3.0 94 3.5 95 3.5 96 2.5 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38968848032845577 2 0.024530385037843692 3 0.047033465527105266 4 0.03973516915220962 5 0.061711150458839834 6 0.05822418663527859 7 0.033531617233548326 8 0.03036902213776021 9 0.034545269507839385 10-11 0.03209223100405502 12-13 0.03199086577662591 14-15 0.02540212599373401 16-17 0.03117994395719306 18-19 0.02722670008745792 20-21 0.039613530879294694 22-23 0.026071136494766108 24-25 0.04727674207293512 26-27 0.04012035701644022 28-29 0.03219359623148412 30-31 0.03922834301506409 32-33 0.036450935783506576 34-35 0.03501154955401327 36-37 0.03582247137344612 38-39 0.018630928801469715 40-41 0.01364375961195769 42-43 0.013501848293556943 44-45 0.01019734187936808 46-47 0.01644143988900102 48-49 0.014393862294933077 50-51 0.010704168016513612 52-53 0.004338431733965744 54-55 0.005027715280483666 56-57 0.008839047831818058 58-59 0.00377078646036275 60-61 0.006811743283235935 62-63 0.00606164060026055 64-65 0.008433586922101634 66-67 0.005027715280483666 68-69 0.006548193691920259 70-71 0.01854983661952643 72-73 0.015002053659507714 74-75 0.010805533243942718 76-77 0.008068672103356852 78-79 0.007196931147466538 80-81 0.008960686104732985 82-83 0.011210994153659143 84-85 0.004966896144026203 86-87 6.487374555462796E-4 88-89 0.024570931128815338 90-91 0.020658233350051836 92-93 0.013522121339042762 94-95 0.025341306857276542 96-97 0.0196648541212466 98-99 0.018610655755983894 100-101 0.10973799521475035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2466329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.751113495426914 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83682663537105 38.48803395704731 2 13.210950475150032 13.409408938936066 3 4.3845259996569945 6.675587298967269 4 2.0710313078590485 4.2042857983094795 5 1.1330490045071542 2.875174931181153 6 0.6884941432525911 2.0965106643089375 7 0.4514020654176487 1.603641021785685 8 0.3485215350776449 1.4150284785860774 9 0.22935669798115976 1.047609702916037 >10 1.4357270047343684 13.70054678344962 >50 0.13324293917638633 4.6972148171938715 >100 0.07117531869738909 6.444903332179908 >500 0.0040921201273986674 1.4156422466028378 >1k 0.0013640400424656134 0.977316014223237 >5k 1.6047529911360157E-4 0.532311455746923 >10k+ 8.023764955680079E-5 0.41678455856561786 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10235 0.41498924109476065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7992 0.3240443590453666 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5080 0.20597414213594373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.054609097164247E-5 0.0 0.0 0.0 0.0 7 4.054609097164247E-5 0.0 0.0 0.0 0.0 8 4.054609097164247E-5 0.0 0.0 0.0 0.0 9 4.054609097164247E-5 0.0 0.0 0.0 0.0 10-11 4.054609097164247E-5 0.0 0.0 0.0 0.0 12-13 4.054609097164247E-5 0.0 0.0 0.0 4.054609097164247E-5 14-15 4.054609097164247E-5 0.0 0.0 2.0273045485821236E-5 4.054609097164247E-5 16-17 4.054609097164247E-5 0.0 0.0 8.109218194328494E-5 4.054609097164247E-5 18-19 4.054609097164247E-5 0.0 0.0 8.109218194328494E-5 4.054609097164247E-5 20-21 4.054609097164247E-5 0.0 0.0 8.109218194328494E-5 4.054609097164247E-5 22-23 4.054609097164247E-5 0.0 0.0 1.2163827291492742E-4 4.054609097164247E-5 24-25 4.054609097164247E-5 0.0 0.0 2.635495913156761E-4 4.054609097164247E-5 26-27 4.054609097164247E-5 0.0 0.0 7.501026829753857E-4 4.054609097164247E-5 28-29 4.054609097164247E-5 0.0 0.0 0.003101775959330649 4.054609097164247E-5 30-31 4.054609097164247E-5 0.0 0.0 0.011271813290116606 4.054609097164247E-5 32-33 4.054609097164247E-5 0.0 0.0 0.027449703587801952 8.109218194328494E-5 34-35 4.054609097164247E-5 0.0 0.0 0.05287210262702178 8.109218194328494E-5 36-37 4.054609097164247E-5 0.0 0.0 0.09516167551044488 8.109218194328494E-5 38-39 4.054609097164247E-5 0.0 0.0 0.15366968478252496 1.2163827291492741E-4 40-41 4.054609097164247E-5 0.0 0.0 0.2198814513392171 1.2163827291492741E-4 42-43 4.054609097164247E-5 4.054609097164247E-5 0.0 0.2841470055292704 1.2163827291492741E-4 44-45 4.054609097164247E-5 4.054609097164247E-5 0.0 0.3640228047434061 1.2163827291492741E-4 46-47 4.054609097164247E-5 4.054609097164247E-5 0.0 0.4598534907548831 1.2163827291492741E-4 48-49 4.054609097164247E-5 8.109218194328494E-5 0.0 0.5536568722177779 1.2163827291492741E-4 50-51 4.054609097164247E-5 8.109218194328494E-5 0.0 0.6570899502864378 1.621843638865699E-4 52-53 4.054609097164247E-5 8.109218194328494E-5 0.0 0.7554547669836424 2.4327654582985482E-4 54-55 4.054609097164247E-5 8.109218194328494E-5 0.0 0.8629221811039809 2.4327654582985482E-4 56-57 4.054609097164247E-5 8.109218194328494E-5 0.0 0.9885136978886434 2.4327654582985482E-4 58-59 4.054609097164247E-5 8.109218194328494E-5 0.0 1.1177949089517254 2.4327654582985482E-4 60-61 4.054609097164247E-5 8.109218194328494E-5 0.0 1.257232915803204 2.4327654582985482E-4 62-63 4.054609097164247E-5 8.109218194328494E-5 0.0 1.3918864839200285 2.838226368014973E-4 64-65 4.054609097164247E-5 8.109218194328494E-5 0.0 1.5309393029072762 2.838226368014973E-4 66-67 4.054609097164247E-5 8.109218194328494E-5 0.0 1.6908328126539485 2.838226368014973E-4 68-69 4.054609097164247E-5 8.109218194328494E-5 0.0 1.8704317226128389 2.838226368014973E-4 70-71 4.054609097164247E-5 8.109218194328494E-5 0.0 2.053558142486262 3.0409568228731854E-4 72-73 4.054609097164247E-5 8.109218194328494E-5 0.0 2.2404958949110196 3.243687277731398E-4 74-75 4.054609097164247E-5 8.109218194328494E-5 0.0 2.424717059240677 3.44641773258961E-4 76-77 4.054609097164247E-5 8.109218194328494E-5 0.0 2.6263324965971693 3.6491481874478224E-4 78-79 4.054609097164247E-5 8.109218194328494E-5 0.0 2.843477086795801 3.6491481874478224E-4 80-81 4.054609097164247E-5 8.109218194328494E-5 0.0 3.0798607971604763 3.6491481874478224E-4 82-83 4.054609097164247E-5 1.2163827291492741E-4 0.0 3.3184137233921343 3.6491481874478224E-4 84-85 4.054609097164247E-5 1.2163827291492741E-4 0.0 3.5587912237175168 3.6491481874478224E-4 86-87 4.054609097164247E-5 1.2163827291492741E-4 0.0 3.8019258582289712 3.6491481874478224E-4 88-89 4.054609097164247E-5 1.2163827291492741E-4 0.0 4.064279339860984 3.6491481874478224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5980 0.0 45.787933 1 GTATCAA 8710 0.0 37.789513 1 ATCAACG 10935 0.0 29.683674 3 TATCAAC 11080 0.0 29.380999 2 TCAACGC 11105 0.0 29.314854 4 CAACGCA 11215 0.0 28.942575 5 AACGCAG 11540 0.0 28.329353 6 TACCGTA 105 6.902116E-5 27.146828 7 TACATGG 8020 0.0 26.369467 2 GTACATG 8470 0.0 25.568945 1 ACATGGG 8460 0.0 24.155376 3 ACGCAGA 13455 0.0 24.115381 7 CGCAGAG 13440 0.0 24.071602 8 GTACCGT 265 3.092282E-11 23.310497 6 TGACGCG 175 0.0 23.089323 94-95 GAGTACT 8800 0.0 23.050262 12-13 GTACTTT 9115 0.0 22.019606 14-15 GCAGAGT 14515 0.0 21.89474 9 CATGGGG 5890 0.0 21.462587 4 GTGGTAT 2915 0.0 21.110159 1 >>END_MODULE