FastQCFastQC Report
Thu 26 May 2016
SRR938223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466329
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97490.3952838408825424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82580.33482961924382354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50140.20329810013181537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61400.043.6881641
GTATCAA87950.037.5839161
ATCAACG110350.029.8655073
TCAACGC111200.029.6366164
TATCAAC111950.029.3566862
CAACGCA114450.028.7938655
AACGCAG119200.027.8114936
GTAAGGT9450.025.664844
CGCAGAG138350.023.6872718
ACGCAGA139550.023.5176187
TAAGGTG11300.023.1455145
TACATGG80800.023.0742592
GAGTACT86500.022.84138112-13
GTACATG85650.022.4340171
GTACTTT88750.022.26230214-15
GCAGAGT149100.021.692759
ACATGGG84900.021.3415663
GTGTAAG9000.021.1383251
GGACCGA8600.020.4337656
AGTACTT86250.020.1267412-13
Produced by FastQC (version 0.11.5)