##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938223_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2466329 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46962793690542 33.0 31.0 34.0 31.0 34.0 2 32.700173415631085 34.0 31.0 34.0 31.0 34.0 3 32.766951205617744 34.0 31.0 34.0 31.0 34.0 4 36.23133572203871 37.0 35.0 37.0 35.0 37.0 5 36.15243019078152 37.0 35.0 37.0 35.0 37.0 6 36.20509753564914 37.0 37.0 37.0 35.0 37.0 7 36.17293759267316 37.0 37.0 37.0 35.0 37.0 8 36.16603908075525 37.0 37.0 37.0 35.0 37.0 9 37.85101136142015 39.0 38.0 39.0 35.0 39.0 10-11 37.82010571176838 39.0 38.0 39.0 35.0 39.0 12-13 37.73160535354367 39.0 38.0 39.0 35.0 39.0 14-15 38.97735541365325 40.0 38.0 41.0 35.5 41.0 16-17 38.88111784761887 40.0 38.0 41.0 35.0 41.0 18-19 39.125334454567906 40.0 38.5 41.0 36.0 41.0 20-21 39.09204814118473 40.0 39.0 41.0 36.0 41.0 22-23 38.98084744573818 40.0 38.5 41.0 35.0 41.0 24-25 38.87139509773432 40.0 38.0 41.0 35.0 41.0 26-27 38.706069830910636 40.0 38.0 41.0 34.5 41.0 28-29 38.5803812062381 40.0 38.0 41.0 34.0 41.0 30-31 38.3440905491522 40.0 38.0 41.0 34.0 41.0 32-33 38.12275957506075 40.0 38.0 41.0 34.0 41.0 34-35 38.03946756495179 40.0 38.0 41.0 33.0 41.0 36-37 37.99350411076543 40.0 38.0 41.0 33.0 41.0 38-39 37.80777564550391 40.0 37.0 41.0 33.0 41.0 40-41 37.58275274709903 40.0 37.0 41.0 33.0 41.0 42-43 37.31642412670816 40.0 37.0 41.0 32.0 41.0 44-45 37.03832396245595 40.0 36.0 41.0 31.0 41.0 46-47 36.67238940952322 39.0 36.0 41.0 30.5 41.0 48-49 36.26148843078113 39.0 35.0 40.5 30.0 41.0 50-51 35.86597286898869 39.0 35.0 40.0 29.0 41.0 52-53 35.312908780620916 38.0 34.5 40.0 27.5 41.0 54-55 34.912137229055816 38.0 34.0 40.0 26.5 41.0 56-57 34.503950405643366 38.0 34.0 40.0 26.0 41.0 58-59 33.975447314612126 37.0 33.0 40.0 24.5 41.0 60-61 33.46548919466949 36.5 32.5 39.0 23.0 41.0 62-63 32.86999504121307 36.0 32.0 39.0 21.5 40.0 64-65 32.26272184286849 35.0 31.0 39.0 19.0 40.0 66-67 31.664735929391416 35.0 31.0 38.0 16.0 40.0 68-69 31.010803505939393 35.0 30.0 37.5 10.0 40.0 70-71 30.365594168499012 34.0 29.0 37.0 7.5 39.0 72-73 29.669744385278687 34.0 28.5 36.0 4.0 39.0 74-75 28.992344898024555 34.0 27.5 36.0 2.0 38.5 76-77 27.732737400403597 31.5 26.0 34.5 2.0 36.0 78-79 28.380303479381702 33.0 27.0 35.0 2.0 37.0 80-81 28.111839093648904 33.0 27.0 35.0 2.0 36.5 82-83 27.5576074805916 33.0 26.0 35.0 2.0 36.0 84-85 26.899748979150793 33.0 25.0 35.0 2.0 35.5 86-87 26.17189636905701 32.0 23.5 35.0 2.0 35.0 88-89 25.358395615507906 32.0 20.5 34.0 2.0 35.0 90-91 24.68704702413993 31.0 18.5 34.0 2.0 35.0 92-93 23.711746486377123 31.0 6.0 34.0 2.0 35.0 94-95 22.787786828115795 30.0 2.0 34.0 2.0 35.0 96-97 21.713806430528933 29.5 2.0 34.0 2.0 35.0 98-99 20.28148799288335 29.0 2.0 34.0 2.0 35.0 100-101 16.492799622434802 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2031560797463925 1101 2 0.1458693516799272 1101 3 0.1559630197321411 1101 4 0.09153172712417756 1101 5 0.06421341637987865 1101 6 0.1058682444847392 1101 7 0.1703428286954889 1101 8 0.0759309870569993 1101 9 0.15322925366832862 1101 10-11 0.21133502359200662 1101 12-13 0.23787415927797895 1101 14-15 0.4093937049345229 1101 16-17 0.454271975975054 1101 18-19 0.31304742108928707 1101 20-21 0.29421376646702413 1101 22-23 0.34941351797488807 1101 24-25 0.3758775130085965 1101 26-27 0.45801376000525096 1101 28-29 0.4961853020918454 1101 30-31 0.5551537905165134 1101 32-33 0.5651925197145147 1101 34-35 0.5390239165347381 1101 36-37 0.5304347948581025 1101 38-39 0.6329066535435288 1101 40-41 0.8199384229593392 1101 42-43 0.8039422455483702 1101 44-45 0.7017797979999969 1101 46-47 0.8179100765074523 1101 48-49 0.9620596324606296 1101 50-51 1.0110790396016753 1101 52-53 1.0103795151731703 1101 54-55 1.2038560932839673 1101 56-57 1.1677664892513988 1101 58-59 1.4813020105974104 1101 60-61 1.5028756431336419 1101 62-63 1.4902981360591454 1101 64-65 1.7609573658518798 1101 66-67 1.9458567229462354 1101 68-69 2.1365634058058696 1101 70-71 2.17035684333284 1101 72-73 2.103738621810873 1101 74-75 2.210915847253869 1101 76-77 2.255550004557687 1101 78-79 2.3167554731447133 1101 80-81 2.3978199183266113 1101 82-83 2.600228238107338 1101 84-85 2.7641278949933756 1101 86-87 3.1060216326396777 1101 88-89 3.593947306789076 1101 90-91 3.7863908129173076 1101 92-93 4.107566365767358 1101 94-95 4.480543068735042 1101 96-97 4.410813899192167 1101 98-99 4.5468618895171105 1101 100-101 4.604301038715615 1102 1 0.20694854901034887 1102 2 0.14828651818552174 1102 3 0.18946842037881595 1102 4 0.08399089203096821 1102 5 0.09108135663442596 1102 6 0.11036594258373356 1102 7 0.11292505709929657 1102 8 0.10745940330479442 1102 9 0.1741598489240772 1102 10-11 0.1927637490325722 1102 12-13 0.2988578107526152 1102 14-15 0.37950979284480724 1102 16-17 0.37877722918417334 1102 18-19 0.3479351554766765 1102 20-21 0.2862602340162681 1102 22-23 0.3615983169058339 1102 24-25 0.3651153161717744 1102 26-27 0.45246247985512156 1102 28-29 0.29419221035158216 1102 30-31 0.3366635640080844 1102 32-33 0.38619713203893724 1102 34-35 0.39115916195436995 1102 36-37 0.3945541366217995 1102 38-39 0.45601129245783767 1102 40-41 0.6508394625220433 1102 42-43 0.7231323956960622 1102 44-45 0.6463250553680382 1102 46-47 0.7277297382021501 1102 48-49 0.9729633011846062 1102 50-51 0.9876061050486058 1102 52-53 1.0602500598740292 1102 54-55 1.0841338043029225 1102 56-57 1.1584068668620233 1102 58-59 1.2260084266031726 1102 60-61 1.2111964059227844 1102 62-63 1.3220147695736628 1102 64-65 1.284307790864112 1102 66-67 1.515231425727439 1102 68-69 1.6625148681649726 1102 70-71 1.7734754675102167 1102 72-73 1.9361373671427273 1102 74-75 2.0669528617538866 1102 76-77 2.02226041245693 1102 78-79 2.1290515746567564 1102 80-81 2.3666088550545865 1102 82-83 2.3970519172627327 1102 84-85 2.7049537627018196 1102 86-87 2.663970989113043 1102 88-89 2.8669890077584164 1102 90-91 3.0046825739695855 1102 92-93 3.1336583412432937 1102 94-95 3.267665377147164 1102 96-97 3.593053872159107 1102 98-99 4.16902435549142 1102 100-101 3.9145937363799668 1103 1 0.16132707022264725 1103 2 0.16939858332511903 1103 3 0.15406042213608373 1103 4 0.14112215968168584 1103 5 0.0028829883905885367 1103 6 0.1050712886408931 1103 7 0.13109378785626546 1103 8 0.12247759449128637 1103 9 0.1954662582398612 1103 10-11 0.2350419772179535 1103 12-13 0.22992135925679236 1103 14-15 0.39499077630799917 1103 16-17 0.37216462776080306 1103 18-19 0.31356134588578755 1103 20-21 0.3188002266589507 1103 22-23 0.3989813990819826 1103 24-25 0.395592239229849 1103 26-27 0.42927162611667313 1103 28-29 0.5236761408237243 1103 30-31 0.4558729955799592 1103 32-33 0.5805138509866339 1103 34-35 0.5957555037927094 1103 36-37 0.519404652879139 1103 38-39 0.5309395463856532 1103 40-41 0.6212456622645703 1103 42-43 0.664964897963074 1103 44-45 0.6476759719498872 1103 46-47 0.7838613173751483 1103 48-49 1.02654829797752 1103 50-51 1.0920923321299796 1103 52-53 1.1110897410352578 1103 54-55 1.2588543222709774 1103 56-57 1.2829466774091145 1103 58-59 1.3381700237856649 1103 60-61 1.5315346056179564 1103 62-63 1.4259623652381563 1103 64-65 1.559997253367733 1103 66-67 1.796991081591269 1103 68-69 1.8797683848350921 1103 70-71 1.9332419972785573 1103 72-73 2.1219686763977954 1103 74-75 2.1771250849900206 1103 76-77 2.1091267473503272 1103 78-79 2.2537249968249746 1103 80-81 2.5026851972032276 1103 82-83 2.7755976267271727 1103 84-85 2.915945293623569 1103 86-87 3.140395836097621 1103 88-89 3.268370362067852 1103 90-91 3.6863488548058996 1103 92-93 3.505956150829274 1103 94-95 3.7452449592500265 1103 96-97 3.8499508448402757 1103 98-99 4.269096295499494 1103 100-101 3.925413623831826 1104 1 0.25407882961039974 1104 2 0.1898307193480946 1104 3 0.17889213917711544 1104 4 0.11793760308683687 1104 5 -0.0010620768752431786 1104 6 0.04383867903995764 1104 7 0.07644054026291514 1104 8 0.12209693029896584 1104 9 0.12855519833077977 1104 10-11 0.20276903886544773 1104 12-13 0.19081869824202613 1104 14-15 0.3769143503832382 1104 16-17 0.3564122060289563 1104 18-19 0.3001424014527103 1104 20-21 0.333475227596594 1104 22-23 0.3434742186610791 1104 24-25 0.3611542128683283 1104 26-27 0.4382163646570163 1104 28-29 0.4224720510712672 1104 30-31 0.3080307757644576 1104 32-33 0.4693770164191591 1104 34-35 0.46152459045771366 1104 36-37 0.4351435996012327 1104 38-39 0.496912730149603 1104 40-41 0.5587718824682781 1104 42-43 0.6560887911079831 1104 44-45 0.646559982081854 1104 46-47 0.6271367075221193 1104 48-49 0.817516774187844 1104 50-51 0.9504704629431302 1104 52-53 0.9474318810060041 1104 54-55 1.0861346631790596 1104 56-57 1.06486601758602 1104 58-59 1.1449477603629532 1104 60-61 1.2740509486588465 1104 62-63 1.415741270680705 1104 64-65 1.536909554447007 1104 66-67 1.7697257429895963 1104 68-69 1.9720346958800796 1104 70-71 1.9957069806194312 1104 72-73 2.022386131039987 1104 74-75 2.0870972301922386 1104 76-77 2.1367994295036503 1104 78-79 1.9361093505020293 1104 80-81 2.104907065848753 1104 82-83 2.353072939619988 1104 84-85 2.507096194786598 1104 86-87 2.630016115349317 1104 88-89 2.6980408642468596 1104 90-91 2.9235993109649456 1104 92-93 3.268853687783089 1104 94-95 3.5948508973794304 1104 96-97 3.857791193234039 1104 98-99 3.9536758964613448 1104 100-101 3.551266831676795 1105 1 0.21542483065390172 1105 2 0.1701166455444607 1105 3 0.1670455675979099 1105 4 0.07658201682470178 1105 5 0.00644525018186215 1105 6 0.0014694960284771241 1105 7 0.13306135313541034 1105 8 0.09057038757754299 1105 9 0.16214704929011248 1105 10-11 0.18910581668915682 1105 12-13 0.2114106876180415 1105 14-15 0.32866856757853213 1105 16-17 0.2614194250335089 1105 18-19 0.19988349477673495 1105 20-21 0.22487338747534835 1105 22-23 0.2585387137237589 1105 24-25 0.30636646363850417 1105 26-27 0.2783285897681367 1105 28-29 0.27585267342843167 1105 30-31 0.37666858233964007 1105 32-33 0.45998364805552683 1105 34-35 0.3533093687439077 1105 36-37 0.4354086702876643 1105 38-39 0.4602291173173043 1105 40-41 0.5659621641945591 1105 42-43 0.570602172572876 1105 44-45 0.4592952471333902 1105 46-47 0.5743152411550909 1105 48-49 0.8200565106271611 1105 50-51 0.897890815447937 1105 52-53 0.9284998888251792 1105 54-55 1.0183693011610728 1105 56-57 0.9364140577498432 1105 58-59 1.0302147358745088 1105 60-61 1.0699236330288642 1105 62-63 1.1777525654092145 1105 64-65 1.265357812229574 1105 66-67 1.2664279973943628 1105 68-69 1.4873640245518622 1105 70-71 1.7265632145585812 1105 72-73 1.6948872402883168 1105 74-75 1.6591530535134602 1105 76-77 1.7102089643524714 1105 78-79 1.818456809935821 1105 80-81 2.1023727092410525 1105 82-83 2.189060657555604 1105 84-85 2.452118145411415 1105 86-87 2.7382936154528075 1105 88-89 2.7138245607309877 1105 90-91 2.7940535128231616 1105 92-93 2.927514678697012 1105 94-95 3.1878588852439904 1105 96-97 3.6152205127327584 1105 98-99 3.6762760949133018 1105 100-101 3.4994823962408823 1106 1 0.15450320024930164 1106 2 0.1278917923671088 1106 3 0.16896954074635318 1106 4 0.11088790047447361 1106 5 -0.025697537194950826 1106 6 0.021511885878354065 1106 7 0.06847822619329946 1106 8 0.1378574650918054 1106 9 0.22711092443121572 1106 10-11 0.17443201132805797 1106 12-13 0.11140446099839352 1106 14-15 0.10329816219594079 1106 16-17 0.14898306910946957 1106 18-19 0.10729043384726111 1106 20-21 -0.0397412039537528 1106 22-23 0.049740186205937675 1106 24-25 0.0285092300855041 1106 26-27 -0.01129085040120259 1106 28-29 0.11309424255053102 1106 30-31 0.21122895508566586 1106 32-33 0.3023249407645494 1106 34-35 0.23254242361769428 1106 36-37 0.3397977918075483 1106 38-39 0.28341718843959995 1106 40-41 0.3171526345711939 1106 42-43 0.2997600894145407 1106 44-45 0.3802169159093225 1106 46-47 0.5453999182987559 1106 48-49 0.5071594546147864 1106 50-51 0.6813479079339899 1106 52-53 0.6706430110790151 1106 54-55 0.8628768296151534 1106 56-57 0.8083565607602523 1106 58-59 0.9034566315017116 1106 60-61 0.8340201243548577 1106 62-63 0.8005117672019253 1106 64-65 0.9635920161501517 1106 66-67 1.2182016944785552 1106 68-69 1.1441048722930454 1106 70-71 1.2655831027654614 1106 72-73 1.562019723220054 1106 74-75 1.9078353473094474 1106 76-77 1.7101699825739622 1106 78-79 1.784809604972942 1106 80-81 1.6098393331643877 1106 82-83 2.0843378973596423 1106 84-85 2.284655041185129 1106 86-87 2.2868300284454826 1106 88-89 2.3935164320098394 1106 90-91 2.354635295373555 1106 92-93 2.3326077396264857 1106 94-95 2.7427684265880465 1106 96-97 3.1844726451319723 1106 98-99 3.5470008315813573 1106 100-101 3.3137978831869574 1107 1 0.23983192612403315 1107 2 0.2170872278719429 1107 3 0.19198924096524195 1107 4 0.1345173296903539 1107 5 0.0550269708551383 1107 6 0.09873635503912226 1107 7 0.14540685873365788 1107 8 0.17171339682438003 1107 9 0.1495086732950881 1107 10-11 0.2693163989323679 1107 12-13 0.251746298129909 1107 14-15 0.3802817953474218 1107 16-17 0.37566267110504015 1107 18-19 0.29576475022854964 1107 20-21 0.2725150433378758 1107 22-23 0.31988277112355235 1107 24-25 0.3399911632180945 1107 26-27 0.37784333442307627 1107 28-29 0.39916269886671785 1107 30-31 0.44978785574011937 1107 32-33 0.5228697523735661 1107 34-35 0.49590854435236764 1107 36-37 0.4576581291446402 1107 38-39 0.38123285861370704 1107 40-41 0.528886931618942 1107 42-43 0.6055711262038344 1107 44-45 0.6376114425151727 1107 46-47 0.7274505521946892 1107 48-49 0.8500327087620576 1107 50-51 0.9367194953960762 1107 52-53 1.030752567851863 1107 54-55 1.1423445181450234 1107 56-57 1.1048383272243214 1107 58-59 1.2612383846322999 1107 60-61 1.324009596460094 1107 62-63 1.3686534965544723 1107 64-65 1.431425686524257 1107 66-67 1.6112282836628857 1107 68-69 1.8606801953521916 1107 70-71 1.9370419523082347 1107 72-73 2.169363971489478 1107 74-75 2.0275894556884317 1107 76-77 1.882898290666276 1107 78-79 1.836328927409859 1107 80-81 1.8936952648969623 1107 82-83 2.10025543533229 1107 84-85 2.295689886016735 1107 86-87 2.4876530931462 1107 88-89 2.7397702792748113 1107 90-91 2.950178718078252 1107 92-93 3.104208674081317 1107 94-95 3.2916995258224837 1107 96-97 3.3340881964158733 1107 98-99 3.308959992238698 1107 100-101 3.2062300213218258 1108 1 0.21360262661355733 1108 2 0.20537141622759236 1108 3 0.153562420305839 1108 4 0.09969826730246467 1108 5 0.12160269616789066 1108 6 0.09345201623966659 1108 7 0.12863382057879846 1108 8 0.1646309651895166 1108 9 0.18569764229209085 1108 10-11 0.20439772538386336 1108 12-13 0.23994513855532063 1108 14-15 0.3586048676414535 1108 16-17 0.3537000448190426 1108 18-19 0.3107867092383998 1108 20-21 0.3804584764656056 1108 22-23 0.29653360261491457 1108 24-25 0.31111680115770923 1108 26-27 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56-57 0.6363430088111102 2316 58-59 0.759166415833711 2316 60-61 0.8862745002561283 2316 62-63 0.8658818963477799 2316 64-65 0.9664566679503039 2316 66-67 1.1719719009047758 2316 68-69 1.5271530848857644 2316 70-71 1.5920602931062149 2316 72-73 1.572015381178737 2316 74-75 2.0099061823357793 2316 76-77 2.00911902931664 2316 78-79 2.0349555015447187 2316 80-81 2.033368118109852 2316 82-83 2.225916826907035 2316 84-85 2.403209699119909 2316 86-87 2.5852746614434032 2316 88-89 2.713698160381295 2316 90-91 3.108931028222493 2316 92-93 3.3465341400344 2316 94-95 3.7416321263259285 2316 96-97 4.13111782068588 2316 98-99 4.34384209611126 2316 100-101 4.961975520479115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 6.0 4 20.0 5 50.0 6 76.0 7 249.0 8 604.0 9 1165.0 10 2087.0 11 3447.0 12 5035.0 13 7523.0 14 10290.0 15 14169.0 16 18623.0 17 25253.0 18 26713.0 19 22847.0 20 23067.0 21 24845.0 22 27102.0 23 29840.0 24 33711.0 25 37786.0 26 42914.0 27 49666.0 28 59761.0 29 71310.0 30 84766.0 31 101142.0 32 122504.0 33 152449.0 34 197184.0 35 266839.0 36 364182.0 37 430048.0 38 201835.0 39 7214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59183223325031 18.25875623244101 14.486875027622023 23.66253650668666 2 16.220060924376565 19.42885019026702 39.3194993690207 25.03158951633571 3 19.822889001520526 22.840141915864166 29.08115560060821 28.2558134820071 4 13.614269263830547 15.944012845256825 34.96723810759524 35.474479783317385 5 14.448163712000417 36.54162783220916 33.61694665363345 15.393261802156976 6 34.344647449711694 34.57580071434103 16.216449630199378 14.863102205747895 7 30.1369768591295 31.80694870797854 19.384356263904774 18.671718168987187 8 27.170388607160216 34.63807022686828 18.646112975561575 19.54542819040993 9 26.447769133801696 16.66063205679372 19.476720259138176 37.41487855026641 10-11 25.912499913839554 25.9120133607479 26.04064583435543 22.134840891057113 12-13 26.534091761480322 23.009663349861274 26.896269719084522 23.55997516957389 14-15 23.31583160961729 24.584883968214292 25.02361303451669 27.075671387651727 16-17 23.024568706074636 27.754514453366614 25.833237543173993 23.387679297384757 18-19 23.023955846928775 26.267298482886915 28.066612361935494 22.64213330824882 20-21 23.43578249292775 25.32433831820491 28.576702459404242 22.6631767294631 22-23 23.349906591696747 25.077296257890037 28.51488400239628 23.057913148016937 24-25 23.296939668148973 25.420449719064962 28.39030254620502 22.892308066581045 26-27 22.723722881092023 25.648574959775782 28.527161078528053 23.100541080604142 28-29 22.861953940451578 26.078921344232665 28.288865759596547 22.770258955719207 30-31 22.890883576359844 25.58239391419393 28.31400433599897 23.212718173447257 32-33 22.738673550852297 25.611141092692826 28.35809820993063 23.29208714652425 34-35 23.3460682454227 25.69406892910561 28.118324993091193 22.841537832380496 36-37 23.409214483319836 25.302100747147666 28.150725394546434 23.13795937498606 38-39 22.843509132744575 25.697305540794602 28.214525170472964 23.244660155987855 40-41 23.328257801855575 25.6295821708947 28.075569324596124 22.966590702653605 42-43 23.02385767505512 25.60383182543906 27.922106490281053 23.450204009224766 44-45 22.993808169421104 25.80481531858196 28.091277650717178 23.110098861279763 46-47 23.394607937546045 26.11450053906028 27.482059368397323 23.00883215499635 48-49 23.24065443012672 26.046504744500837 27.474720525931456 23.23812029944099 50-51 23.27692290850085 26.27167746071185 27.430626651999795 23.0207729787875 52-53 23.446952940990435 26.14055140250956 27.23677579106437 23.175719865435635 54-55 23.25671068215149 26.15186376189065 27.282106320770666 23.309319235187196 56-57 23.118534290951104 26.400436924764886 27.27290788716913 23.20812089711488 58-59 23.283471102192774 25.983009566039243 27.28462017841091 23.448899153357075 60-61 23.175172493207516 26.21274371748457 27.305987968352962 23.30609582095495 62-63 22.99981064975516 26.295579381339635 27.443763585474606 23.260846383430597 64-65 22.9311255716492 26.25562120868708 27.329322243707143 23.483930975956575 66-67 22.897132316315528 26.215303435856175 27.228996461744654 23.658567786083644 68-69 23.01107727739011 26.01386281529897 27.443853367986993 23.53120653932393 70-71 23.042607048775732 26.087253565927337 27.167137879820576 23.703001505476358 72-73 23.08516485131644 26.195192570657156 26.93300993764618 23.786632640380226 74-75 22.900817368647896 26.59993861321827 26.788437390145436 23.710806627988397 76-77 23.133247835142836 26.259168991647098 26.99082320323039 23.616759969979675 78-79 23.028314551708227 26.30283713162356 27.107210757364484 23.561637559303726 80-81 22.96830468952414 26.416651157774297 26.938094583564954 23.676949569136614 82-83 23.10182969193195 26.532894860883122 26.798577377067858 23.566698070117074 84-85 22.939266914656113 26.528403225322496 26.795267602344303 23.737062257677092 86-87 23.0305527026112 26.389967331140273 26.89046139848021 23.68901856776832 88-89 22.970941832983353 26.589376356520155 26.499282942381164 23.940398868115327 90-91 22.979762961881708 26.400252202719088 26.715120404497476 23.904864430901725 92-93 22.927516969552723 26.239159495752595 26.76131205528541 24.072011479409277 94-95 22.837772827099066 26.091232372329966 26.880360827845927 24.190633972725045 96-97 22.879564859087044 26.00631911212116 26.728749527384338 24.385366501407457 98-99 22.637085319922846 26.143855908923747 26.594647348346466 24.624411422806933 100-101 23.165704980965636 25.451490859491983 26.62002920129472 24.762774958247665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1025.0 1 698.0 2 503.0 3 650.5 4 556.0 5 510.5 6 1016.5 7 2287.0 8 3676.0 9 4207.5 10 3493.5 11 2635.0 12 2536.5 13 2652.0 14 2758.5 15 2877.0 16 2938.5 17 3029.5 18 3211.0 19 3399.5 20 3685.0 21 4232.0 22 5112.5 23 6141.5 24 7483.0 25 9348.5 26 11786.0 27 14452.0 28 16376.0 29 18471.0 30 21341.0 31 24974.0 32 30240.5 33 33329.0 34 34728.5 35 37499.5 36 42110.5 37 47571.0 38 51728.0 39 54708.5 40 57631.5 41 63448.0 42 68864.0 43 72145.5 44 76402.5 45 81610.0 46 86498.5 47 92750.0 48 96037.0 49 96200.0 50 96239.0 51 93790.0 52 93550.5 53 97390.0 54 101138.0 55 97259.5 56 88638.0 57 81503.0 58 75788.0 59 67932.0 60 56686.0 61 47291.5 62 39318.0 63 30243.5 64 22176.5 65 16768.5 66 12726.0 67 9827.0 68 7367.0 69 5489.5 70 4274.5 71 2982.5 72 2088.0 73 1586.0 74 1134.5 75 805.5 76 531.0 77 305.0 78 178.5 79 108.0 80 70.0 81 35.5 82 20.0 83 11.5 84 9.0 85 6.0 86 3.0 87 2.5 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013015295201897233 3 0.003203141186759755 4 0.001986758457610481 5 0.11681328808930194 6 0.0 7 0.0 8 3.243687277731398E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0273045485821236E-5 16-17 2.0273045485821236E-5 18-19 0.0 20-21 0.0 22-23 2.635495913156761E-4 24-25 4.662800461738884E-4 26-27 0.006203551918661299 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011089355880744216 36-37 6.487374555462796E-4 38-39 0.007115838965523253 40-41 0.010095976651938975 42-43 0.00573727187248741 44-45 0.0038721516877918555 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.0273045485821236E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0273045485821236E-5 68-69 7.703757284612068E-4 70-71 0.0 72-73 2.0273045485821236E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.109218194328494E-5 82-83 0.0016015705933798774 84-85 0.0038518786423060347 86-87 0.002676042004128403 88-89 0.0 90-91 0.002392219367326906 92-93 0.0 94-95 2.0273045485821236E-5 96-97 6.0819136457463705E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2466329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.81256166385599 #Duplication Level Percentage of deduplicated Percentage of total 1 74.45023302756482 36.341065905464546 2 13.811079865617993 13.483083751698361 3 4.7329187021181 6.93077657991471 4 2.158417395906645 4.214315289361303 5 1.2096844841787209 2.9523899238891813 6 0.749136957324322 2.194037635444015 7 0.4984099610539544 1.7030066870478346 8 0.3518838262985019 1.374108077576737 9 0.2552171943486755 1.1212024533138938 >10 1.5520285821071413 14.309071194689166 >50 0.14591210306965172 4.952684894360392 >100 0.07852094960469225 6.881135562144326 >500 0.004896963260721633 1.60371101416843 >1k 0.0014109894141057042 1.0067354231775425 >5k 2.489981319010066E-4 0.9326756077496161 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9749 0.3952838408825424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8258 0.33482961924382354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5014 0.20329810013181537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.054609097164247E-5 2 0.0 0.0 0.0 0.0 4.054609097164247E-5 3 0.0 0.0 0.0 0.0 4.054609097164247E-5 4 0.0 0.0 0.0 0.0 4.054609097164247E-5 5 0.0 0.0 0.0 0.0 4.054609097164247E-5 6 0.0 0.0 0.0 0.0 4.054609097164247E-5 7 0.0 0.0 0.0 0.0 4.054609097164247E-5 8 0.0 0.0 0.0 0.0 4.054609097164247E-5 9 0.0 0.0 0.0 0.0 4.054609097164247E-5 10-11 0.0 0.0 0.0 0.0 4.054609097164247E-5 12-13 0.0 0.0 0.0 0.0 8.109218194328494E-5 14-15 0.0 0.0 0.0 2.0273045485821236E-5 8.109218194328494E-5 16-17 0.0 0.0 0.0 8.109218194328494E-5 8.109218194328494E-5 18-19 0.0 0.0 0.0 8.109218194328494E-5 8.109218194328494E-5 20-21 0.0 0.0 0.0 8.109218194328494E-5 8.109218194328494E-5 22-23 0.0 0.0 0.0 1.2163827291492742E-4 8.109218194328494E-5 24-25 0.0 0.0 0.0 2.635495913156761E-4 8.109218194328494E-5 26-27 0.0 0.0 0.0 7.501026829753857E-4 8.109218194328494E-5 28-29 0.0 0.0 0.0 0.003101775959330649 8.109218194328494E-5 30-31 0.0 0.0 0.0 0.01133263242657407 8.109218194328494E-5 32-33 0.0 0.0 0.0 0.027551068815231058 8.109218194328494E-5 34-35 0.0 0.0 0.0 0.0531761983093091 8.109218194328494E-5 36-37 0.0 0.0 0.0 0.09570904773856205 8.109218194328494E-5 38-39 0.0 0.0 0.0 0.1544400605109862 8.109218194328494E-5 40-41 0.0 0.0 0.0 0.22103701493190892 8.109218194328494E-5 42-43 0.0 0.0 0.0 0.2858296683045936 8.109218194328494E-5 44-45 0.0 0.0 0.0 0.3660501092919882 8.109218194328494E-5 46-47 0.0 0.0 0.0 0.4621646179402667 8.109218194328494E-5 48-49 0.0 0.0 0.0 0.556819467313566 8.109218194328494E-5 50-51 0.0 0.0 0.0 0.6608404637013148 8.109218194328494E-5 52-53 0.0 0.0 0.0 0.7594485569443492 8.109218194328494E-5 54-55 0.0 0.0 0.0 0.8675647085202339 8.109218194328494E-5 56-57 0.0 0.0 0.0 0.993663051442042 8.109218194328494E-5 58-59 0.0 0.0 0.0 1.1233699964603263 8.109218194328494E-5 60-61 0.0 0.0 0.0 1.2635378329492943 8.109218194328494E-5 62-63 0.0 0.0 0.0 1.3983941315209771 8.109218194328494E-5 64-65 0.0 0.0 0.0 1.5386836062828602 8.109218194328494E-5 66-67 0.0 0.0 0.0 1.6992866726215359 8.109218194328494E-5 68-69 0.0 0.0 0.0 1.8798181426727738 8.109218194328494E-5 70-71 0.0 0.0 0.0 2.065194870595123 1.2163827291492741E-4 72-73 0.0 0.0 0.0 2.2550316685243534 1.4191131840074866E-4 74-75 0.0 0.0 0.0 2.4422329705404264 1.621843638865699E-4 76-77 0.0 0.0 0.0 2.6476597404482534 1.621843638865699E-4 78-79 0.0 0.0 0.0 2.8687981206075914 1.621843638865699E-4 80-81 0.0 0.0 0.0 3.110898829799268 1.621843638865699E-4 82-83 0.0 0.0 0.0 3.3555133966311876 2.838226368014973E-4 84-85 0.0 0.0 0.0 3.6023782715120327 2.838226368014973E-4 86-87 0.0 0.0 0.0 3.8545749573556485 3.0409568228731854E-4 88-89 0.0 0.0 0.0 4.1275515148222315 3.243687277731398E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6140 0.0 43.688164 1 GTATCAA 8795 0.0 37.583916 1 ATCAACG 11035 0.0 29.865507 3 TCAACGC 11120 0.0 29.636616 4 TATCAAC 11195 0.0 29.356686 2 CAACGCA 11445 0.0 28.793865 5 AACGCAG 11920 0.0 27.811493 6 GTAAGGT 945 0.0 25.66484 4 CGCAGAG 13835 0.0 23.687271 8 ACGCAGA 13955 0.0 23.517618 7 TAAGGTG 1130 0.0 23.145514 5 TACATGG 8080 0.0 23.074259 2 GAGTACT 8650 0.0 22.841381 12-13 GTACATG 8565 0.0 22.434017 1 GTACTTT 8875 0.0 22.262302 14-15 GCAGAGT 14910 0.0 21.69275 9 ACATGGG 8490 0.0 21.341566 3 GTGTAAG 900 0.0 21.138325 1 GGACCGA 860 0.0 20.433765 6 AGTACTT 8625 0.0 20.12674 12-13 >>END_MODULE