FastQCFastQC Report
Thu 26 May 2016
SRR938222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476288
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.41255298252868805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82910.3348156595678693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.2038938927943761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.043.4949951
GTATCAA88500.042.46911
ATCAACG110850.033.617273
TATCAAC111250.033.4950452
TCAACGC111900.033.3018264
CAACGCA113900.032.7588045
AACGCAG116950.031.8580276
CGCAGAG135100.027.1828848
ACGCAGA136450.027.053767
TACATGG81250.025.3296412
GCAGAGT146000.024.402599
GTACATG85300.024.242671
ACATGGG86000.023.2683563
GAGTACT85850.022.65947712-13
GTACTAG3800.022.6219731
GTACTTT88950.021.81614514-15
CATGGGG60350.021.7377134
AGAGTAC130350.020.89911810-11
AGTACTT87700.020.82730312-13
CAGAGTA139800.020.53972810-11
Produced by FastQC (version 0.11.5)