##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2476288 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894566787061926 33.0 31.0 34.0 30.0 34.0 2 32.08367403145353 34.0 31.0 34.0 30.0 34.0 3 32.169325619637135 34.0 31.0 34.0 30.0 34.0 4 35.68401252196836 37.0 35.0 37.0 35.0 37.0 5 35.68440423731004 37.0 35.0 37.0 35.0 37.0 6 35.7075752093456 37.0 35.0 37.0 35.0 37.0 7 35.65169641011062 37.0 35.0 37.0 33.0 37.0 8 35.62023278390882 37.0 35.0 37.0 33.0 37.0 9 37.25785207536442 39.0 37.0 39.0 34.0 39.0 10-11 37.16644085825236 39.0 37.0 39.0 34.0 39.0 12-13 37.060942830559284 39.0 37.0 39.0 33.0 39.0 14-15 38.26302251595938 40.0 38.0 41.0 33.5 41.0 16-17 38.11302441396154 40.0 38.0 41.0 33.0 41.0 18-19 38.23958178531738 40.0 38.0 41.0 33.5 41.0 20-21 38.21411584597591 40.0 38.0 41.0 34.0 41.0 22-23 38.124935790990385 40.0 38.0 41.0 33.0 41.0 24-25 38.015818030859094 40.0 38.0 41.0 33.0 41.0 26-27 37.837512437971675 40.0 38.0 41.0 33.0 41.0 28-29 37.6292135648196 40.0 37.5 41.0 32.0 41.0 30-31 37.42379299176832 40.0 37.0 41.0 32.0 41.0 32-33 37.1748084229298 40.0 36.5 41.0 31.0 41.0 34-35 36.93811745645095 39.5 36.0 41.0 30.5 41.0 36-37 36.68831210263103 39.0 36.0 41.0 30.0 41.0 38-39 36.39478263433009 39.0 35.0 41.0 30.0 41.0 40-41 36.189203557906026 39.0 35.0 40.0 30.0 41.0 42-43 36.0285277802905 39.0 35.0 40.5 29.0 41.0 44-45 35.76074047122144 39.0 35.0 40.0 27.5 41.0 46-47 35.38096739959165 39.0 35.0 40.0 26.5 41.0 48-49 34.99867079273493 38.0 34.5 40.0 25.0 41.0 50-51 34.30039579402719 37.5 33.5 39.5 24.0 40.5 52-53 34.42125390907681 38.0 34.0 40.0 24.0 40.5 54-55 34.5198631984648 38.0 34.0 40.0 24.0 41.0 56-57 34.20346583273028 38.0 34.0 40.0 23.0 41.0 58-59 33.87103741568025 37.5 33.5 40.0 22.5 41.0 60-61 33.4361186986328 37.0 33.0 40.0 21.0 41.0 62-63 32.87383212291947 36.0 32.0 39.0 18.5 41.0 64-65 32.329538809702264 36.0 31.5 39.0 15.0 40.5 66-67 31.781602543807505 35.0 31.0 38.5 10.0 40.0 68-69 31.165976049635585 35.0 30.5 38.0 7.5 40.0 70-71 30.613222896529 35.0 30.0 37.0 2.0 39.5 72-73 29.96457439522382 34.0 29.0 36.5 2.0 39.0 74-75 29.271790478328853 34.0 28.5 36.0 2.0 39.0 76-77 28.60086084494211 34.0 27.5 35.5 2.0 38.0 78-79 27.877649732179776 33.5 26.0 35.0 2.0 37.0 80-81 27.224574847513697 33.0 25.5 35.0 2.0 36.5 82-83 26.513327407797476 32.5 24.5 35.0 2.0 36.0 84-85 25.860855037863125 32.0 22.0 35.0 2.0 35.5 86-87 25.18037401142355 31.5 19.0 34.5 2.0 35.0 88-89 24.372745617634138 31.0 14.5 34.0 2.0 35.0 90-91 23.545942354039596 30.5 4.5 34.0 2.0 35.0 92-93 22.64451489487491 30.0 2.0 34.0 2.0 35.0 94-95 21.69308295319446 29.0 2.0 34.0 2.0 35.0 96-97 20.490341390016024 29.0 2.0 34.0 2.0 35.0 98-99 19.009240443760984 26.0 2.0 33.5 2.0 35.0 100-101 15.422700227114131 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3165729129617212 1101 2 0.30575106216556946 1101 3 0.2256444186481943 1101 4 0.22807377144547303 1101 5 0.15438489287043922 1101 6 0.25593765884037367 1101 7 0.20458725474038886 1101 8 0.19739940661327182 1101 9 0.2071236568971031 1101 10-11 0.29427028445051917 1101 12-13 0.36460480389362715 1101 14-15 0.48396512074961606 1101 16-17 0.6092219037156212 1101 18-19 0.4912423467742215 1101 20-21 0.46526127290243835 1101 22-23 0.5320904549271361 1101 24-25 0.4726142313744077 1101 26-27 0.6862339905053432 1101 28-29 0.6806097864910541 1101 30-31 0.7377395530942508 1101 32-33 0.6852154541536919 1101 34-35 0.7405932131690491 1101 36-37 0.8091951292936059 1101 38-39 0.9232430709662012 1101 40-41 1.0318515291119255 1101 42-43 1.1727209975610222 1101 44-45 1.060886136304937 1101 46-47 1.0001131671902428 1101 48-49 1.074506670138959 1101 50-51 0.9784694819780881 1101 52-53 1.0796944070757135 1101 54-55 1.2848280462799266 1101 56-57 1.2029535967931295 1101 58-59 1.1753628120897943 1101 60-61 1.3210016488435201 1101 62-63 1.4651291761568288 1101 64-65 1.3346675165727078 1101 66-67 1.4488182708280242 1101 68-69 1.7414722896545207 1101 70-71 1.947586675753243 1101 72-73 2.064235345708525 1101 74-75 2.285847982117822 1101 76-77 2.2078046511966143 1101 78-79 2.157327523458875 1101 80-81 2.377608434667234 1101 82-83 2.55584080006118 1101 84-85 2.556756138976308 1101 86-87 2.926740771692522 1101 88-89 3.2074611162544677 1101 90-91 3.5424079524161627 1101 92-93 3.8797044599996333 1101 94-95 4.175501643884697 1101 96-97 4.311872439812202 1101 98-99 4.576715693538304 1101 100-101 4.288562648272094 1102 1 0.37399638961761283 1102 2 0.30830674365999045 1102 3 0.214566572946012 1102 4 0.1474598659367814 1102 5 0.22335267936070835 1102 6 0.36771407813007784 1102 7 0.12700865650188575 1102 8 0.33941392777719415 1102 9 0.39108046502821026 1102 10-11 0.35125303960059284 1102 12-13 0.4283814833193489 1102 14-15 0.5627737520006235 1102 16-17 0.5795122031197835 1102 18-19 0.4905918131857092 1102 20-21 0.5237680689558033 1102 22-23 0.528517262253061 1102 24-25 0.4785820689628224 1102 26-27 0.5214507937434192 1102 28-29 0.5350058141521785 1102 30-31 0.5649447864734114 1102 32-33 0.5440556656071109 1102 34-35 0.4984907799682645 1102 36-37 0.5064171321551072 1102 38-39 0.49992996785324095 1102 40-41 0.710351287557053 1102 42-43 0.8582376540080574 1102 44-45 0.7735777156402293 1102 46-47 0.862269919268229 1102 48-49 0.8514458447645552 1102 50-51 0.804013251410872 1102 52-53 0.7161672956383995 1102 54-55 0.783801050974759 1102 56-57 0.993163772756553 1102 58-59 0.9377601125592818 1102 60-61 0.8646195291685714 1102 62-63 0.7960119275906763 1102 64-65 0.8650000725948601 1102 66-67 0.9464300131818852 1102 68-69 1.1360297177764629 1102 70-71 1.3695481942043202 1102 72-73 1.5352677295454065 1102 74-75 1.4893055071714016 1102 76-77 1.567288815416763 1102 78-79 1.9413165001952848 1102 80-81 1.8962387411322332 1102 82-83 2.3567902965121803 1102 84-85 2.3166440172574276 1102 86-87 2.3023241421881444 1102 88-89 2.694381539409047 1102 90-91 2.830397294581733 1102 92-93 3.1808326064066605 1102 94-95 3.5715610478016053 1102 96-97 3.5474323748401346 1102 98-99 3.8363269837509932 1102 100-101 3.83920633006381 1103 1 0.4993863994782366 1103 2 0.44951882807873034 1103 3 0.44173857833256136 1103 4 0.35249237289072965 1103 5 0.3796022194434201 1103 6 0.40906432170227447 1103 7 0.45456450885739486 1103 8 0.3730830927145732 1103 9 0.5874665269588348 1103 10-11 0.47251874117681325 1103 12-13 0.6138790034453265 1103 14-15 0.7925219840785616 1103 16-17 0.8037991473557327 1103 18-19 0.7422652222398298 1103 20-21 0.79229330737693 1103 22-23 0.7542467370643138 1103 24-25 0.7083022460861699 1103 26-27 0.7456717398484685 1103 28-29 0.8759073164260087 1103 30-31 0.9119047791883261 1103 32-33 0.8844280330405496 1103 34-35 0.8577765560143007 1103 36-37 1.024926733388419 1103 38-39 1.10081535462529 1103 40-41 1.1048720169490807 1103 42-43 1.1648274868925128 1103 44-45 1.1237640281337846 1103 46-47 1.139415847344523 1103 48-49 1.3051988618219639 1103 50-51 1.2257944282840185 1103 52-53 1.3155630103130775 1103 54-55 1.4332084015568327 1103 56-57 1.546137276001737 1103 58-59 1.4170033554544759 1103 60-61 1.4048409057561955 1103 62-63 1.4517070373821284 1103 64-65 1.6658075953699623 1103 66-67 1.7347629693830378 1103 68-69 1.8512976839854183 1103 70-71 1.8968461249444601 1103 72-73 2.2028997626005804 1103 74-75 2.224713941318605 1103 76-77 2.491056375058836 1103 78-79 2.6691519362704597 1103 80-81 2.8614381796652104 1103 82-83 2.70829956108604 1103 84-85 2.6780971535254032 1103 86-87 2.722757071530129 1103 88-89 3.1371770270685495 1103 90-91 3.242575858388822 1103 92-93 3.5011081980442604 1103 94-95 3.9192079269868536 1103 96-97 4.058523970127638 1103 98-99 4.319209552149893 1103 100-101 4.235135326193149 1104 1 0.37237446441432454 1104 2 0.38220761170474304 1104 3 0.3159027691105436 1104 4 0.2552494570390351 1104 5 0.1719854664407663 1104 6 0.24337331680843022 1104 7 0.23303114135071468 1104 8 0.25439954760302186 1104 9 0.342876642858549 1104 10-11 0.32833291287397515 1104 12-13 0.32731425229155775 1104 14-15 0.5044672916030422 1104 16-17 0.5649459991462464 1104 18-19 0.4401721674486936 1104 20-21 0.4502151226116098 1104 22-23 0.43946955445141356 1104 24-25 0.47710010819459825 1104 26-27 0.5278964963843364 1104 28-29 0.5390124397838321 1104 30-31 0.5573031527215235 1104 32-33 0.547656898355136 1104 34-35 0.5971011621286451 1104 36-37 0.7716290469746809 1104 38-39 0.7186397380250966 1104 40-41 0.7448764376541064 1104 42-43 0.7570568068722139 1104 44-45 0.8280451010090886 1104 46-47 0.7260411995210561 1104 48-49 0.8906480881566097 1104 50-51 0.8658498571238908 1104 52-53 0.9112981424913116 1104 54-55 1.1411291042684084 1104 56-57 1.3222049518318144 1104 58-59 1.2207960516773184 1104 60-61 1.299715553789575 1104 62-63 1.3651680631788068 1104 64-65 1.3617171417837923 1104 66-67 1.4155953087924225 1104 68-69 1.4711001544584228 1104 70-71 1.7442394413254547 1104 72-73 1.879442485796858 1104 74-75 1.9491802765793267 1104 76-77 2.0664176406987096 1104 78-79 2.0492649770144133 1104 80-81 2.2609888882584634 1104 82-83 2.477032036223246 1104 84-85 2.3567700436043424 1104 86-87 2.7487647130944985 1104 88-89 2.7920556717887024 1104 90-91 3.0076482374628064 1104 92-93 3.2786444043740524 1104 94-95 3.572428597414266 1104 96-97 3.6208686104741084 1104 98-99 3.6409135555118155 1104 100-101 3.6481597391482907 1105 1 0.23050460788054394 1105 2 0.15612291351329333 1105 3 0.07820728136745458 1105 4 -0.05909248718626969 1105 5 0.10980186250347401 1105 6 0.11328402860517173 1105 7 0.06260868355562366 1105 8 0.12507741378930604 1105 9 0.09871255214124375 1105 10-11 0.2215037111130016 1105 12-13 0.14342280928375573 1105 14-15 0.22519616229539707 1105 16-17 0.2904492286441638 1105 18-19 0.20849232927716344 1105 20-21 0.2509529515757052 1105 22-23 0.24617657218951194 1105 24-25 0.23068503120905604 1105 26-27 0.29693884799996084 1105 28-29 0.40630292009240776 1105 30-31 0.28034041632859186 1105 32-33 0.32331148768185614 1105 34-35 0.25553122185517907 1105 36-37 0.3637846689123023 1105 38-39 0.45324337781122637 1105 40-41 0.6955614509285013 1105 42-43 0.6029846912097128 1105 44-45 0.6477796476359643 1105 46-47 0.8050565267019394 1105 48-49 0.7920668591692888 1105 50-51 0.7362080100252797 1105 52-53 0.6983942150734705 1105 54-55 0.8611379900928071 1105 56-57 0.8335821774418193 1105 58-59 0.7617668366553332 1105 60-61 0.9999214302457418 1105 62-63 1.0168590235927937 1105 64-65 1.0108509471483345 1105 66-67 1.2428276044330353 1105 68-69 1.3393064637087342 1105 70-71 1.4980669054526032 1105 72-73 1.6070803920748773 1105 74-75 1.6357553425024776 1105 76-77 1.7291292597882055 1105 78-79 1.9562772343177741 1105 80-81 2.209072254363722 1105 82-83 2.365080334663471 1105 84-85 2.314816337143032 1105 86-87 2.4717935507603137 1105 88-89 2.686597378544217 1105 90-91 2.707957823868785 1105 92-93 2.772061933072262 1105 94-95 3.030537022426305 1105 96-97 3.2378364244809816 1105 98-99 3.5370630421042506 1105 100-101 3.347010850246452 1106 1 0.25173504376471456 1106 2 0.20061895023275866 1106 3 0.12932596261737217 1106 4 0.1544532149195632 1106 5 0.16104829751407834 1106 6 0.14294881991411046 1106 7 0.12886702895885094 1106 8 0.11420852871616205 1106 9 0.3413445495352505 1106 10-11 0.1616256921827457 1106 12-13 0.21730949583891146 1106 14-15 0.2858028991054766 1106 16-17 0.0982915458427911 1106 18-19 0.22966164886646112 1106 20-21 0.24058633969132615 1106 22-23 0.08517316992129054 1106 24-25 0.11549032404889914 1106 26-27 0.18374050422854538 1106 28-29 0.1950948204119598 1106 30-31 0.08678066284274877 1106 32-33 0.15091208188253802 1106 34-35 0.2462656234737466 1106 36-37 0.3139044169751415 1106 38-39 0.11991725267327524 1106 40-41 0.5016267179169986 1106 42-43 0.41953949281869996 1106 44-45 0.3442692258123756 1106 46-47 0.6581179239630686 1106 48-49 0.7644446710556352 1106 50-51 0.7366472498459302 1106 52-53 0.7241142831188441 1106 54-55 0.31690942343172424 1106 56-57 0.7900387727565601 1106 58-59 0.9629214975358096 1106 60-61 1.0265669078853108 1106 62-63 0.7476208869020908 1106 64-65 0.900553671968872 1106 66-67 1.2332795828219467 1106 68-69 1.0295156332694226 1106 70-71 1.035138961027478 1106 72-73 1.309866712331008 1106 74-75 1.4828256558411965 1106 76-77 1.6501086510975114 1106 78-79 1.7436199039511493 1106 80-81 1.892570900756649 1106 82-83 2.2094898270286123 1106 84-85 2.393962092374803 1106 86-87 2.1319896351458887 1106 88-89 2.447120193556156 1106 90-91 2.540271120872813 1106 92-93 2.767809327846411 1106 94-95 2.828505492246048 1106 96-97 2.9200116001922467 1106 98-99 2.958955113641448 1106 100-101 3.0304230749777403 1107 1 0.2067548496405074 1107 2 0.10930217751073457 1107 3 0.07775199454935233 1107 4 0.037795571094001446 1107 5 0.053293239546022164 1107 6 -0.01734875634368649 1107 7 0.007976933080065862 1107 8 0.10236272167479399 1107 9 0.10118772959862099 1107 10-11 0.12339162489305977 1107 12-13 0.07762400841320272 1107 14-15 0.09280425401079384 1107 16-17 0.2363793987320264 1107 18-19 0.24177969425283408 1107 20-21 0.37441430221547733 1107 22-23 0.4026740056434619 1107 24-25 0.21158173282501735 1107 26-27 0.2649171566657529 1107 28-29 0.3422044050358579 1107 30-31 0.41142241167642624 1107 32-33 0.2728864718053572 1107 34-35 0.24353199845118922 1107 36-37 0.3529015799572264 1107 38-39 0.3663800031310771 1107 40-41 0.4438386008508033 1107 42-43 0.45661212816871455 1107 44-45 0.3838002155736646 1107 46-47 0.30629989543905367 1107 48-49 0.30147197032700035 1107 50-51 0.2904260452818477 1107 52-53 0.2659636992966412 1107 54-55 0.43088979801274974 1107 56-57 0.5361974830523621 1107 58-59 0.44930736155419737 1107 60-61 0.5630555731260714 1107 62-63 0.8467847949460321 1107 64-65 0.8992846315554246 1107 66-67 0.882861943611335 1107 68-69 1.0524948126937907 1107 70-71 1.1012865924577326 1107 72-73 1.2999917304506496 1107 74-75 1.4582885571727289 1107 76-77 1.611298823004823 1107 78-79 1.7946212174401523 1107 80-81 1.9149035418457778 1107 82-83 1.8816551757688167 1107 84-85 2.0656197953665405 1107 86-87 2.1725385049241055 1107 88-89 2.4134974636881843 1107 90-91 2.6394453090389867 1107 92-93 2.9236499903891975 1107 94-95 3.1779167148853915 1107 96-97 3.292251276434957 1107 98-99 3.360652960880312 1107 100-101 3.5545991120867626 1108 1 0.20257771331943175 1108 2 0.10070517117919309 1108 3 0.03553439649800083 1108 4 0.021409212445092862 1108 5 0.08338854780016192 1108 6 0.1211504297222703 1108 7 0.10543140463683187 1108 8 0.03183729496802101 1108 9 0.0468592728133288 1108 10-11 0.07688469675140652 1108 12-13 0.13172885384334876 1108 14-15 0.253459310386404 1108 16-17 0.28198750660175165 1108 18-19 0.2907188280745956 1108 20-21 0.16456820525323934 1108 22-23 0.1607595424553594 1108 24-25 0.07286331025529336 1108 26-27 0.1613004952559649 1108 28-29 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-0.9839666868290351 2316 50-51 -1.071116529792839 2316 52-53 -1.0388940185326376 2316 54-55 -1.1002234404723552 2316 56-57 -1.0012314972494565 2316 58-59 -0.9603828780640811 2316 60-61 -0.9917531633384371 2316 62-63 -1.2019527456448529 2316 64-65 -1.2580519107735952 2316 66-67 -1.3674511368736049 2316 68-69 -1.2634265914080451 2316 70-71 -1.3551411864391412 2316 72-73 -1.3555224951418339 2316 74-75 -1.350230919563252 2316 76-77 -1.6153555037261427 2316 78-79 -2.331796699569079 2316 80-81 -2.457020195514371 2316 82-83 -2.2085039332887 2316 84-85 -2.102374803232326 2316 86-87 -1.9949059801285394 2316 88-89 -2.2067879733286055 2316 90-91 -2.3642657264985836 2316 92-93 -2.855462360985335 2316 94-95 -2.8653015805893567 2316 96-97 -3.0935681427569435 2316 98-99 -3.285392737125461 2316 100-101 -2.969404053165798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6902.0 3 2199.0 4 2255.0 5 2461.0 6 2930.0 7 3557.0 8 4312.0 9 5583.0 10 6898.0 11 8871.0 12 11004.0 13 13180.0 14 15183.0 15 18433.0 16 21939.0 17 26494.0 18 26646.0 19 22947.0 20 23971.0 21 25606.0 22 28264.0 23 31347.0 24 35047.0 25 39136.0 26 44734.0 27 51374.0 28 59517.0 29 69102.0 30 81917.0 31 98017.0 32 120846.0 33 151928.0 34 199557.0 35 270385.0 36 355720.0 37 389065.0 38 188928.0 39 10033.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2303713182402 17.888855259645258 14.116844220958736 23.7639292011558 2 15.933127646414572 19.1124408804973 39.91260797250254 25.041823500585593 3 19.748868871522067 22.898856628909858 28.815109080462648 28.537165419105428 4 13.312206118433892 15.618528645586055 35.25996939611265 35.8092958398674 5 14.527566231422082 36.49672768335825 33.497989888616345 15.477716196603323 6 34.192763536793606 34.458780854997926 16.368511988360225 14.979943619848237 7 30.058813761539426 31.86944694378128 19.464700632418637 18.60703866226065 8 27.174564263001212 34.46874284174494 18.735953660360465 19.62073923489338 9 26.69066794443641 16.69404552297117 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23.335696797808644 34-35 23.430042792910637 25.674675699715504 28.02682117338997 22.86846033398389 36-37 23.45271062865237 25.298749611898714 28.061727749145877 23.18681201030304 38-39 22.927234683696675 25.71585818559316 28.10967309331362 23.24723403739654 40-41 23.32420185758614 25.631744090324865 28.06410021303186 22.979953839057135 42-43 23.063445919718024 25.582653626309177 27.856354912289415 23.49754554168338 44-45 23.014909759905002 25.84956147171023 27.98212363609927 23.153405132285496 46-47 23.400500364659884 26.1370551598818 27.446801162306674 23.01564331315165 48-49 23.276161892393546 26.08041703105718 27.38371987340417 23.259701203145102 50-51 23.284450288392936 26.28593400597082 27.334795307760828 23.094820397875417 52-53 23.449922916701166 26.137640035332705 27.255883028870798 23.15655401909533 54-55 23.246203581263433 26.14841717268081 27.305033430456817 23.30034581559894 56-57 23.155261556133482 26.37145466302461 27.27321011045308 23.200073670388825 58-59 23.275369050263535 25.998505624103878 27.284941133706255 23.441184191926332 60-61 23.163271900382494 26.138327133492467 27.42032093769059 23.27808002843445 62-63 23.020277910222724 26.217616705875084 27.536627001845616 23.22547838205658 64-65 22.970032134392522 26.250983499821473 27.41537054718941 23.363613818596587 66-67 22.925675878974346 26.200665696439916 27.305730671813027 23.567927752772707 68-69 23.002079771211033 26.001158726789875 27.52105366997127 23.47570783202783 70-71 23.0889081191554 26.078355145773614 27.223217955100132 23.60951877997086 72-73 23.06758070502876 26.12566183030891 27.11338386582005 23.69337359884228 74-75 22.98414985794465 26.549659396321807 26.94608964602379 23.520101099709752 76-77 23.305816498792435 26.253140069303655 26.900741500609836 23.54030193129407 78-79 23.231085042211625 26.265394545658328 27.045832504516003 23.457687907614048 80-81 23.200224409637254 26.351592466282515 26.924329805424808 23.523853318655423 82-83 23.35432709269317 26.44177690123711 26.696008250755433 23.50788775531429 84-85 23.257090421196587 26.455645640090697 26.731883490189208 23.555380448523515 86-87 23.30476072976951 26.347257387459404 26.767194093061303 23.580787789709785 88-89 23.318327504091442 26.425663179354768 26.518953939732025 23.73705537682176 90-91 23.387611004836778 26.30488688903283 26.585212793396433 23.722289312733956 92-93 23.431137674970724 26.062082610273485 26.674996369975347 23.83178334478045 94-95 23.29704031389227 25.92935808093641 26.844991656227695 23.928609948943627 96-97 23.393792042345602 25.79085961587944 26.651085441316123 24.164262900458834 98-99 23.255945463747587 25.914010157082146 26.452703889912836 24.377340489257428 100-101 24.206918140047666 24.887719450085154 26.43151887425428 24.4738435356129 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1053.0 1 720.0 2 523.5 3 707.5 4 588.0 5 557.5 6 1089.0 7 2278.0 8 3778.0 9 4408.0 10 3589.0 11 2675.5 12 2598.0 13 2686.5 14 2765.5 15 2836.0 16 2906.0 17 3065.5 18 3224.0 19 3468.0 20 3783.0 21 4337.0 22 5171.5 23 6218.0 24 7534.5 25 9410.5 26 11826.5 27 14494.0 28 16547.5 29 18624.0 30 21510.5 31 25067.0 32 30244.5 33 33305.5 34 35068.0 35 37865.0 36 41811.0 37 47183.5 38 51821.0 39 54950.0 40 57684.5 41 63345.0 42 68551.5 43 72014.0 44 76297.5 45 81241.5 46 86442.5 47 92456.0 48 96083.5 49 96662.5 50 96075.0 51 93578.0 52 93259.0 53 96858.5 54 100584.5 55 97048.0 56 88821.0 57 81673.0 58 75679.0 59 68108.0 60 57326.0 61 47819.5 62 39920.5 63 31122.0 64 23251.0 65 17976.0 66 13551.0 67 10382.0 68 8066.5 69 6111.0 70 4776.5 71 3394.5 72 2417.0 73 1819.5 74 1337.0 75 981.0 76 669.5 77 400.5 78 247.5 79 162.0 80 102.0 81 65.5 82 42.0 83 31.0 84 26.5 85 16.5 86 10.0 87 6.5 88 6.0 89 5.0 90 6.0 91 5.0 92 2.0 93 2.0 94 0.5 95 2.0 96 2.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46448555256900653 2 0.0405849400392846 3 0.05354789103690686 4 0.05811117285226921 5 0.08266405200041352 6 0.08129102915331335 7 0.04793465057376202 8 0.04034264188979634 9 0.04611741445260002 10-11 0.05025667450635791 12-13 0.046036648402770594 14-15 0.039736896516075676 16-17 0.03971670500361832 18-19 0.04765196939935904 20-21 0.055143020521037936 22-23 0.04795484208621937 24-25 0.06412824356456115 26-27 0.0559304895068748 28-29 0.04347232632068645 30-31 0.04535013697922051 32-33 0.044744391605499846 34-35 0.04961054610772253 36-37 0.04427998681898067 38-39 0.02657203039387987 40-41 0.024310580998656055 42-43 0.02378560167476481 44-45 0.01587052879148144 46-47 0.028005627778352117 48-49 0.02798543626589476 50-51 0.016657997777318308 52-53 0.015951294841310866 54-55 0.016233976015713844 56-57 0.015951294841310866 58-59 0.013649462421172334 60-61 0.016880104414349218 62-63 0.016819529876977154 64-65 0.017606998862814017 66-67 0.009469819342499741 68-69 0.011892800837382405 70-71 0.017990637599503773 72-73 0.0157089966918226 74-75 0.012781227385506046 76-77 0.00920732968055412 78-79 0.014961910730900443 80-81 0.018515616923395015 82-83 0.0158907203039388 84-85 0.01381099452083118 86-87 0.0032912165305489503 88-89 0.028046010803266828 90-91 0.03135741884627313 92-93 0.016536848702574176 94-95 0.03186220665770702 96-97 0.02661241341879458 98-99 0.023301005375788275 100-101 0.14889221286053964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2476288.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.50116872899877 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85765824980186 38.30900398659969 2 13.074405086385926 13.205454745977107 3 4.458090290368097 6.754163098889713 4 2.057951743502611 4.1571587293905035 5 1.1439558171105197 2.888555286920901 6 0.6910963741740189 2.094070476009684 7 0.4774808061475668 1.6879337129281642 8 0.3250776169005316 1.3133439664891655 9 0.24213453770071058 1.100526942918753 >10 1.4602718091738558 13.89497811092652 >50 0.13258744554588775 4.634438506108978 >100 0.07374780127080097 6.651279199124815 >500 0.004016247766470127 1.407972015787958 >1k 0.0012851992852699785 0.9455711108936751 >5k 1.6064991065874732E-4 0.5411376499065477 >10k+ 8.032495532937366E-5 0.4144124611278329 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10216 0.41255298252868805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8291 0.3348156595678693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5049 0.2038938927943761 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.038302491471105E-5 9 0.0 0.0 0.0 0.0 4.038302491471105E-5 10-11 0.0 0.0 0.0 0.0 4.038302491471105E-5 12-13 0.0 0.0 0.0 0.0 1.2114907474413315E-4 14-15 0.0 0.0 0.0 4.038302491471105E-5 1.2114907474413315E-4 16-17 0.0 0.0 0.0 8.07660498294221E-5 1.615320996588442E-4 18-19 0.0 0.0 0.0 1.2114907474413315E-4 1.615320996588442E-4 20-21 0.0 0.0 0.0 2.624896619456218E-4 1.615320996588442E-4 22-23 0.0 0.0 0.0 4.442132740618216E-4 1.615320996588442E-4 24-25 0.0 0.0 0.0 7.6727747337951E-4 1.615320996588442E-4 26-27 0.0 0.0 0.0 0.0011711077225266204 1.8172361211619975E-4 28-29 0.0 0.0 0.0 0.004179643078672594 2.0191512457355527E-4 30-31 0.0 0.0 0.0 0.012195673524242737 2.0191512457355527E-4 32-33 0.0 0.0 0.0 0.029297884575622865 2.422981494882663E-4 34-35 0.0 0.0 0.0 0.05397191279851132 2.422981494882663E-4 36-37 0.0 0.0 0.0 0.09534432182363278 2.422981494882663E-4 38-39 0.0 0.0 0.0 0.1578168613666908 2.422981494882663E-4 40-41 0.0 0.0 0.0 0.21899714411247803 2.422981494882663E-4 42-43 0.0 0.0 0.0 0.28514453892277475 2.624896619456218E-4 44-45 0.0 0.0 0.0 0.3666778662255763 2.8268117440297737E-4 46-47 0.0 0.0 0.0 0.4619414219993797 2.8268117440297737E-4 48-49 0.0 0.0 0.0 0.5612634717771114 2.8268117440297737E-4 50-51 0.0 0.0 0.0 0.6675717848650884 2.8268117440297737E-4 52-53 0.0 0.0 0.0 0.7656621523829216 3.0287268686033286E-4 54-55 0.0 0.0 0.0 0.8704762935490541 3.6344722423239944E-4 56-57 0.0 0.0 0.0 0.9952800320479686 3.6344722423239944E-4 58-59 0.0 0.0 0.0 1.1238192003514937 3.6344722423239944E-4 60-61 0.0 0.0 0.0 1.260919569936938 3.6344722423239944E-4 62-63 0.0 0.0 0.0 1.40040253799235 3.6344722423239944E-4 64-65 0.0 0.0 0.0 1.5399056975602192 3.6344722423239944E-4 66-67 2.0191512457355525E-5 0.0 0.0 1.7005493706709398 3.6344722423239944E-4 68-69 4.038302491471105E-5 0.0 0.0 1.8789211917192183 3.6344722423239944E-4 70-71 4.038302491471105E-5 0.0 0.0 2.061533230383542 3.83638736689755E-4 72-73 4.038302491471105E-5 0.0 0.0 2.250788276646335 4.0383024914711054E-4 74-75 4.038302491471105E-5 0.0 0.0 2.442345155329267 4.0383024914711054E-4 76-77 4.038302491471105E-5 0.0 0.0 2.644179513852993 4.0383024914711054E-4 78-79 4.038302491471105E-5 0.0 0.0 2.8657005970226406 4.0383024914711054E-4 80-81 4.038302491471105E-5 0.0 0.0 3.1091698542334334 4.2402176160446603E-4 82-83 4.038302491471105E-5 0.0 0.0 3.3500949808745997 4.442132740618216E-4 84-85 4.038302491471105E-5 0.0 0.0 3.5911816396154244 4.442132740618216E-4 86-87 4.038302491471105E-5 0.0 0.0 3.8420006073606947 4.442132740618216E-4 88-89 6.0574537372066576E-5 2.0191512457355525E-5 0.0 4.115171579396257 4.442132740618216E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5490 0.0 43.494995 1 GTATCAA 8850 0.0 42.4691 1 ATCAACG 11085 0.0 33.61727 3 TATCAAC 11125 0.0 33.495045 2 TCAACGC 11190 0.0 33.301826 4 CAACGCA 11390 0.0 32.758804 5 AACGCAG 11695 0.0 31.858027 6 CGCAGAG 13510 0.0 27.182884 8 ACGCAGA 13645 0.0 27.05376 7 TACATGG 8125 0.0 25.329641 2 GCAGAGT 14600 0.0 24.40259 9 GTACATG 8530 0.0 24.24267 1 ACATGGG 8600 0.0 23.268356 3 GAGTACT 8585 0.0 22.659477 12-13 GTACTAG 380 0.0 22.621973 1 GTACTTT 8895 0.0 21.816145 14-15 CATGGGG 6035 0.0 21.737713 4 AGAGTAC 13035 0.0 20.899118 10-11 AGTACTT 8770 0.0 20.827303 12-13 CAGAGTA 13980 0.0 20.539728 10-11 >>END_MODULE