FastQCFastQC Report
Thu 26 May 2016
SRR938222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476288
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100280.40496097384472246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83970.3390962602088287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.21136475240359762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64550.040.1078531
GTATCAA93100.036.074381
TATCAAC114450.029.220392
ATCAACG114250.029.1432743
TCAACGC114650.029.0812644
CAACGCA117650.028.339715
AACGCAG121900.027.390626
CGCAGAG142700.022.9987538
ACGCAGA144200.022.9571387
TACATGG81950.022.4331972
GTAAGGT10100.022.1017514
GTACATG85700.021.8950251
GAGTACT88350.021.85265512-13
GGACCGA8850.021.4667826
GTACTTT89600.021.22974414-15
GCAGAGT156450.020.7649449
ACATGGG86250.020.486153
TAAGGTG11450.020.3254815
AGTACTT89200.019.48796512-13
CCAATAC11700.019.4864143
Produced by FastQC (version 0.11.5)