##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938222_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2476288 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.482004920267755 33.0 31.0 34.0 31.0 34.0 2 32.71618042812468 34.0 31.0 34.0 31.0 34.0 3 32.683495619249456 34.0 31.0 34.0 31.0 34.0 4 36.21309758800269 37.0 35.0 37.0 35.0 37.0 5 36.16973025754678 37.0 35.0 37.0 35.0 37.0 6 36.22869835818774 37.0 37.0 37.0 35.0 37.0 7 36.188184896102555 37.0 37.0 37.0 35.0 37.0 8 36.18067728794066 37.0 37.0 37.0 35.0 37.0 9 37.884236001628246 39.0 38.0 39.0 35.0 39.0 10-11 37.84086220988835 39.0 38.0 39.0 35.0 39.0 12-13 37.77918400444536 39.0 38.0 39.0 35.0 39.0 14-15 39.059543154915744 40.0 38.0 41.0 36.0 41.0 16-17 38.991121993887624 40.0 38.0 41.0 36.0 41.0 18-19 38.97342433513388 40.0 38.0 41.0 35.5 41.0 20-21 38.96578346298976 40.0 38.5 41.0 35.0 41.0 22-23 38.99671383134756 40.0 38.5 41.0 35.0 41.0 24-25 38.94633762308746 40.0 38.0 41.0 35.0 41.0 26-27 38.777637940336504 40.0 38.0 41.0 35.0 41.0 28-29 38.69721413664324 40.0 38.0 41.0 34.5 41.0 30-31 38.491585792928774 40.0 38.0 41.0 34.0 41.0 32-33 38.29413905006203 40.0 38.0 41.0 34.0 41.0 34-35 38.0881412016696 40.0 38.0 41.0 33.5 41.0 36-37 38.0223865317766 40.0 38.0 41.0 33.0 41.0 38-39 37.98727793374599 40.0 38.0 41.0 33.0 41.0 40-41 37.86776901555877 40.0 37.5 41.0 33.0 41.0 42-43 37.675409322340535 40.0 37.0 41.0 33.0 41.0 44-45 37.42533360416882 40.0 37.0 41.0 32.5 41.0 46-47 37.079229475731424 40.0 36.5 41.0 31.5 41.0 48-49 36.71079575558255 39.5 36.0 41.0 31.0 41.0 50-51 36.40654237310038 39.0 35.5 41.0 30.0 41.0 52-53 35.92764936873255 39.0 35.0 40.0 29.0 41.0 54-55 35.59690411616096 38.0 35.0 40.0 28.0 41.0 56-57 35.30178214327251 38.0 34.5 40.0 28.0 41.0 58-59 34.90409920009304 38.0 34.0 40.0 26.5 41.0 60-61 34.469380177103794 37.0 34.0 40.0 26.0 41.0 62-63 34.007356575648714 36.5 33.0 39.5 26.0 41.0 64-65 33.54206316066629 36.0 33.0 39.0 24.5 40.5 66-67 33.00565241199731 35.5 32.0 39.0 23.0 40.0 68-69 32.45469165945157 35.0 31.5 38.0 22.0 40.0 70-71 31.84121414795048 35.0 31.0 37.5 20.5 39.5 72-73 31.360629498668978 35.0 31.0 37.0 18.5 39.0 74-75 30.795287139460353 34.0 30.0 36.0 17.5 39.0 76-77 29.621570269693994 33.0 28.5 35.0 16.0 37.0 78-79 30.318768858872637 34.0 30.0 35.0 16.5 37.0 80-81 30.138914980809986 34.0 30.0 35.0 12.0 37.0 82-83 29.688206097190633 34.0 29.5 35.0 7.0 36.5 84-85 29.178856417347255 34.0 29.0 35.0 3.5 36.0 86-87 28.631405353496845 34.0 29.0 35.0 2.0 35.5 88-89 27.999415657629484 33.0 27.0 35.0 2.0 35.0 90-91 27.4552424839114 33.0 26.0 35.0 2.0 35.0 92-93 26.79140734034167 32.5 25.0 35.0 2.0 35.0 94-95 26.017831326566217 32.0 24.0 34.0 2.0 35.0 96-97 25.1596237998165 32.0 19.5 34.0 2.0 35.0 98-99 24.103126130724696 31.0 8.5 34.0 2.0 35.0 100-101 20.615988729905403 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.013372131302375578 1101 2 0.042913281294922 1101 3 0.1627259612974754 1101 4 0.019871447394443464 1101 5 0.03956448824458647 1101 6 0.06646279485737239 1101 7 0.06980392153595716 1101 8 0.07006832725512169 1101 9 0.056663178903676226 1101 10-11 0.11837502828826274 1101 12-13 0.0754087199854041 1101 14-15 0.1689980537712117 1101 16-17 0.11291651723183094 1101 18-19 0.15158923851411998 1101 20-21 0.15197573551269983 1101 22-23 0.06625901942801704 1101 24-25 0.20107213423823822 1101 26-27 0.2711451657871464 1101 28-29 0.21528829467348487 1101 30-31 0.23004937262515313 1101 32-33 0.33660828290700096 1101 34-35 0.1596474427071044 1101 36-37 0.1439434283970158 1101 38-39 0.24258432318343637 1101 40-41 0.17654854701906686 1101 42-43 0.25040469766949514 1101 44-45 0.44963115734912407 1101 46-47 0.5584653817347771 1101 48-49 0.6100342302912978 1101 50-51 0.5079003300336993 1101 52-53 0.4364929529258035 1101 54-55 0.5428253855296603 1101 56-57 0.6987087182546858 1101 58-59 0.6785410007933308 1101 60-61 0.6135578521010032 1101 62-63 0.6940619321643098 1101 64-65 0.665562732725995 1101 66-67 0.6816154091474118 1101 68-69 0.7235979558931476 1101 70-71 0.6969666184916399 1101 72-73 0.7652241095774741 1101 74-75 0.7628724097126032 1101 76-77 0.9311036544647529 1101 78-79 1.0361843222087082 1101 80-81 1.059415558172347 1101 82-83 1.0675032838409955 1101 84-85 1.2939625935954915 1101 86-87 1.3798703593741415 1101 88-89 1.4955698888840026 1101 90-91 1.5738159720771527 1101 92-93 1.7136349326943723 1101 94-95 1.7299291011297768 1101 96-97 2.033743150359985 1101 98-99 2.046212046070771 1101 100-101 2.4151425022558897 1102 1 0.10941245440005076 1102 2 0.11357621005481633 1102 3 0.18945377541170672 1102 4 0.10546974474229387 1102 5 0.003223254828256472 1102 6 0.0434032082258895 1102 7 0.0845643176859383 1102 8 0.12003224886747432 1102 9 0.08434869614078622 1102 10-11 0.09744414394969425 1102 12-13 0.16618380143275147 1102 14-15 0.1265209782574459 1102 16-17 0.09740106855914377 1102 18-19 0.2430487254267959 1102 20-21 0.2810539094817486 1102 22-23 0.2956416918920013 1102 24-25 0.1981374748306024 1102 26-27 0.26261700141834865 1102 28-29 0.018920637322167977 1102 30-31 0.09491664906301622 1102 32-33 0.03810936835549228 1102 34-35 -0.0703017610908816 1102 36-37 0.2250971548454146 1102 38-39 0.21089011771314148 1102 40-41 0.21291610372252023 1102 42-43 0.17949885916440422 1102 44-45 0.23761624133589265 1102 46-47 0.32687290802709157 1102 48-49 0.3165477728483026 1102 50-51 0.36458241904976063 1102 52-53 0.3622423149732015 1102 54-55 0.36758506940246605 1102 56-57 0.29205477192940776 1102 58-59 0.36241129047336074 1102 60-61 0.3702639030505068 1102 62-63 0.276365173335158 1102 64-65 0.26349426430774514 1102 66-67 0.35017367496925544 1102 68-69 0.4590464040579434 1102 70-71 0.6492870162519928 1102 72-73 0.7044058966499449 1102 74-75 0.8001328290890228 1102 76-77 0.6450794618154525 1102 78-79 0.7605535697962615 1102 80-81 0.7647238425753358 1102 82-83 0.8734387205598146 1102 84-85 0.8043163553480674 1102 86-87 0.9875238938173894 1102 88-89 1.0732777809151663 1102 90-91 1.057359078782472 1102 92-93 1.2803404649202683 1102 94-95 1.3776352589208471 1102 96-97 1.6959313742503817 1102 98-99 1.9062075788541044 1102 100-101 2.1176723249917764 1103 1 0.23322356820934687 1103 2 0.21653467400466297 1103 3 0.3422488767406904 1103 4 0.12660064456127174 1103 5 0.13093814658212466 1103 6 0.09496407551951336 1103 7 0.17033245314446077 1103 8 0.17573467134381104 1103 9 0.21929072816574546 1103 10-11 0.2916276619858067 1103 12-13 0.22424743926811885 1103 14-15 0.32917318328489387 1103 16-17 0.3076929596654452 1103 18-19 0.34217009943889565 1103 20-21 0.30731012756348974 1103 22-23 0.3950666113144905 1103 24-25 0.41467803217676646 1103 26-27 0.41708137254584443 1103 28-29 0.4903577040800826 1103 30-31 0.4790678533104469 1103 32-33 0.4682090692252814 1103 34-35 0.4642313584728939 1103 36-37 0.40550454038120165 1103 38-39 0.37912441245757833 1103 40-41 0.44163527086750065 1103 42-43 0.38486043693826133 1103 44-45 0.3628390712621652 1103 46-47 0.46987706016037833 1103 48-49 0.4730323306654327 1103 50-51 0.41265849304861746 1103 52-53 0.41139321567521847 1103 54-55 0.4627419563879087 1103 56-57 0.44285820144065013 1103 58-59 0.6401886849867324 1103 60-61 0.678608320390488 1103 62-63 0.696012563948436 1103 64-65 0.6896375267034358 1103 66-67 0.6790492599840405 1103 68-69 0.742927820823958 1103 70-71 0.8097930908395448 1103 72-73 0.8129167120649043 1103 74-75 0.9130924244353942 1103 76-77 0.41388639957576245 1103 78-79 0.7791558443631992 1103 80-81 0.8237185829612237 1103 82-83 0.9344605463946571 1103 84-85 1.1065406533303168 1103 86-87 1.2913068514902797 1103 88-89 1.5193000482706083 1103 90-91 1.47678823864236 1103 92-93 1.8212077953395642 1103 94-95 1.873299158725107 1103 96-97 2.110282357369581 1103 98-99 2.315685968260553 1103 100-101 2.485725734266655 1104 1 0.17429070037247385 1104 2 0.13764461101905567 1104 3 0.18261293628292208 1104 4 0.09077215280255047 1104 5 0.02609837719766972 1104 6 0.09642399970841353 1104 7 0.11256038771802679 1104 8 0.03965924143759736 1104 9 0.029579719686637418 1104 10-11 0.1074908140870221 1104 12-13 0.030749863938758892 1104 14-15 0.13287950153451078 1104 16-17 0.20722876464728301 1104 18-19 0.20570129815525462 1104 20-21 0.19675146463994508 1104 22-23 0.2738026423274391 1104 24-25 0.1871152092564401 1104 26-27 0.16333836202473861 1104 28-29 0.1762511839809875 1104 30-31 0.1282279193565401 1104 32-33 0.22014580402453987 1104 34-35 0.17764404199197514 1104 36-37 0.24901126778743077 1104 38-39 0.2687967555110049 1104 40-41 0.33658790047329745 1104 42-43 0.3763757689760254 1104 44-45 0.3045018231069534 1104 46-47 0.18698919787141932 1104 48-49 0.32935217560069674 1104 50-51 0.3217679791762933 1104 52-53 0.37109552968129833 1104 54-55 0.36019975712704166 1104 56-57 0.47900596633974857 1104 58-59 0.45908356750639 1104 60-61 0.6252549658394528 1104 62-63 0.39976959645888144 1104 64-65 0.4731974159213266 1104 66-67 0.3859461265732165 1104 68-69 0.42903026614974493 1104 70-71 0.588897534928531 1104 72-73 0.7527654215171111 1104 74-75 0.5628985935229558 1104 76-77 0.5663181231931169 1104 78-79 0.811618219041673 1104 80-81 0.9304499564906479 1104 82-83 0.9749275248196803 1104 84-85 1.05850547409025 1104 86-87 1.0025906275868195 1104 88-89 1.1787255539333508 1104 90-91 1.0246723876230597 1104 92-93 1.3032656314275464 1104 94-95 1.44678246437131 1104 96-97 1.7087725585599394 1104 98-99 1.9841152493649155 1104 100-101 2.2408219297315064 1105 1 0.1336795534664219 1105 2 0.04050437548013974 1105 3 0.13254378844167292 1105 4 0.06205591503729124 1105 5 0.04468742104862855 1105 6 0.01499523096207156 1105 7 0.09874026912171985 1105 8 0.07565549244496594 1105 9 0.09964354737283543 1105 10-11 0.08333513671143322 1105 12-13 0.1278875795700145 1105 14-15 0.07309303850998106 1105 16-17 0.06725883974525715 1105 18-19 0.16178537109461644 1105 20-21 0.15519519486555566 1105 22-23 0.14199233593659244 1105 24-25 0.14656611507662376 1105 26-27 0.15817948105902957 1105 28-29 0.15311795914017523 1105 30-31 0.14076453873345685 1105 32-33 0.06573428829850769 1105 34-35 0.34235064048043995 1105 36-37 0.2539765883456724 1105 38-39 0.19653533210021124 1105 40-41 0.36565015305971116 1105 42-43 0.3748978431309311 1105 44-45 0.3453221223491383 1105 46-47 0.30951080962326927 1105 48-49 0.3584546679626257 1105 50-51 0.46061835635134685 1105 52-53 0.387236483236407 1105 54-55 0.5149816992797369 1105 56-57 0.5158826100873313 1105 58-59 0.5730600936215353 1105 60-61 0.5464443811562987 1105 62-63 0.5649849013070707 1105 64-65 0.6472127108158858 1105 66-67 0.7873321387862546 1105 68-69 0.9982807936372851 1105 70-71 1.0408262013823695 1105 72-73 0.924196551101943 1105 74-75 0.7471684951630841 1105 76-77 0.7936300164170724 1105 78-79 1.0408021674188426 1105 80-81 1.108898713932053 1105 82-83 1.1049138117971644 1105 84-85 1.361861880853116 1105 86-87 1.3576034090309754 1105 88-89 1.3137792964765893 1105 90-91 1.5357028896878866 1105 92-93 1.787091131601656 1105 94-95 1.7889286760287462 1105 96-97 2.022926690917142 1105 98-99 2.4528116964053517 1105 100-101 2.7720753207791233 1106 1 0.04637450447830105 1106 2 0.029215881416320144 1106 3 0.13678358761828235 1106 4 -0.00891797043767184 1106 5 -0.03086320839553025 1106 6 -0.059834272212292205 1106 7 -0.050118780905606286 1106 8 -0.14630186693847236 1106 9 -0.029843400885823712 1106 10-11 0.03553099840978291 1106 12-13 -0.014762990429538547 1106 14-15 -0.012881408284023621 1106 16-17 0.020547586556013187 1106 18-19 -0.04744423231968398 1106 20-21 -0.07453098879680908 1106 22-23 -0.04981996694993995 1106 24-25 -0.02853506038066911 1106 26-27 -0.08800408621858224 1106 28-29 -0.17623292292822157 1106 30-31 -0.07079637910599246 1106 32-33 -0.05473589768520526 1106 34-35 -0.13116345905645943 1106 36-37 -0.09148637410606852 1106 38-39 -0.15890154895352282 1106 40-41 -0.10491449095666638 1106 42-43 -0.16926385601555438 1106 44-45 0.053466202212270275 1106 46-47 -0.2597614816442686 1106 48-49 -0.00715136643182035 1106 50-51 0.05707068196056042 1106 52-53 0.017220796976332053 1106 54-55 0.025840155474455173 1106 56-57 0.31486873906556667 1106 58-59 0.23655546105239011 1106 60-61 0.3024088561021401 1106 62-63 0.36424662873422875 1106 64-65 0.39558542236721195 1106 66-67 0.48424546683153835 1106 68-69 -0.04435246135676607 1106 70-71 0.3021136985681103 1106 72-73 0.27783606096919655 1106 74-75 0.43464476179637757 1106 76-77 0.5918713241002713 1106 78-79 0.7440727403752909 1106 80-81 0.8280307674579639 1106 82-83 0.812405856710047 1106 84-85 0.8278638905593354 1106 86-87 0.983733792095947 1106 88-89 1.0589976557195513 1106 90-91 1.117633922287947 1106 92-93 1.033372546297418 1106 94-95 1.449427121205666 1106 96-97 1.5983668202597734 1106 98-99 2.0306451766631817 1106 100-101 2.6219759243658025 1107 1 0.01297934249611643 1107 2 0.013493506841207648 1107 3 0.10597827686275707 1107 4 0.0255742802730623 1107 5 0.02585215202999791 1107 6 0.0026068944262647165 1107 7 -0.007835972524127044 1107 8 0.03940876315166264 1107 9 -7.006595396106263E-4 1107 10-11 0.020150752219343815 1107 12-13 -0.01474353955077845 1107 14-15 0.038645818508783236 1107 16-17 0.09735089749495529 1107 18-19 0.08300719077465146 1107 20-21 -0.014100214696206592 1107 22-23 -0.01018846865540013 1107 24-25 0.0013858640315902448 1107 26-27 0.07057656171016191 1107 28-29 0.08308693235132125 1107 30-31 0.02163619364620928 1107 32-33 8.459128588000908E-4 1107 34-35 0.061338042285136396 1107 36-37 0.12003986165337466 1107 38-39 0.16922442174147534 1107 40-41 0.1649890090181927 1107 42-43 0.1979249521115065 1107 44-45 0.26180826255583867 1107 46-47 0.22454764976675534 1107 48-49 0.23475196491945383 1107 50-51 0.2931341643039005 1107 52-53 0.2533651151009977 1107 54-55 0.29153339071027773 1107 56-57 0.4347183978246747 1107 58-59 0.42637207550491496 1107 60-61 0.4572987182745507 1107 62-63 0.5307557654702038 1107 64-65 0.4609005118116727 1107 66-67 0.6698413145496431 1107 68-69 0.5446145268780427 1107 70-71 0.6455883355096965 1107 72-73 0.5134096474154077 1107 74-75 0.6837127466853374 1107 76-77 0.6710326288326804 1107 78-79 0.7544203860054246 1107 80-81 0.8982025860189822 1107 82-83 1.0771156022659305 1107 84-85 1.1939985054319386 1107 86-87 1.2666461581215493 1107 88-89 1.3019187191723098 1107 90-91 1.297107624404024 1107 92-93 1.3910652579126257 1107 94-95 1.5578510527385738 1107 96-97 1.4527601303408026 1107 98-99 1.5927045735823988 1107 100-101 1.7310559347790466 1108 1 0.022188828020652807 1108 2 0.07460411225713415 1108 3 0.13344909766249913 1108 4 0.0780780006607884 1108 5 -0.02524526532588567 1108 6 -0.051209986996205714 1108 7 -0.03600535830204876 1108 8 0.018220228931504323 1108 9 -0.058698064264874006 1108 10-11 -0.03760434218924047 1108 12-13 -0.029872904611657702 1108 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-3.921492113216786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 5.0 4 17.0 5 45.0 6 80.0 7 206.0 8 512.0 9 980.0 10 1732.0 11 2789.0 12 4380.0 13 6304.0 14 8784.0 15 12039.0 16 15439.0 17 21087.0 18 21632.0 19 16827.0 20 15629.0 21 16578.0 22 17375.0 23 18838.0 24 21671.0 25 25221.0 26 29710.0 27 35567.0 28 43980.0 29 52873.0 30 64671.0 31 79426.0 32 99992.0 33 130816.0 34 180556.0 35 266120.0 36 402813.0 37 536104.0 38 308220.0 39 17264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.5043096764189 18.29851778145353 14.50651135893725 23.690661183190322 2 16.21128781658459 19.434231947776997 39.33054038433811 25.0239398513003 3 20.018108991574714 22.859532542471726 29.011743382181304 28.11061508377226 4 13.586787966504705 15.96171366173886 35.02068418536132 35.43081418639512 5 14.458467725163441 36.6159063659628 33.54803719613177 15.37758871274198 6 34.25762038083631 34.625106055962014 16.20486639876557 14.91240716443611 7 30.145120438333507 31.849445621833972 19.359097164788587 18.646336775043938 8 27.18893246219037 34.649335880695574 18.657247726131907 19.504483930982154 9 26.504025379923497 16.64455023002171 19.42298311020366 37.428441279851135 10-11 25.981166164840275 25.921217564354386 26.031968010182982 22.06564826062235 12-13 26.561086594127985 22.97574030161274 26.920919537630517 23.54225356662876 14-15 23.302176483510802 24.60442807944795 25.029519991212656 27.063875445828593 16-17 23.025541869742884 27.700928850331163 25.93156609068335 23.341963189242605 18-19 23.05206421870154 26.226311317585033 28.079185458234264 22.64243900547917 20-21 23.38358866173886 25.313816486612218 28.639802801612735 22.662792050036185 22-23 23.374825545332367 25.07474897911713 28.522389156673217 23.028036318877287 24-25 23.259172838294663 25.456345095872752 28.439483719542164 22.84499834629042 26-27 22.74577599334338 25.636903054856546 28.54711539626696 23.070205555533114 28-29 22.873900115616834 26.071858507286926 28.33823920771576 22.716002169380477 30-31 22.90997948338348 25.621540811832883 28.327145638093832 23.141334066689804 32-33 22.718027426337127 25.603925213528083 28.401522253989132 23.276525106145655 34-35 23.354136761402238 25.66089792937767 28.135519012679964 22.84944629654013 36-37 23.37302111364065 25.316094774112436 28.213601596396238 23.09728251585068 38-39 22.845832591976546 25.700094940792773 28.22284205596397 23.23123041126671 40-41 23.304758952256986 25.59530072777323 28.13268153180764 22.967258788162148 42-43 22.988408252157978 25.619140056215876 27.925473369757732 23.46697832186841 44-45 22.966524955084502 25.814521866279545 28.124663651231597 23.09428952740436 46-47 23.365933953657798 26.08464234556469 27.507793093616872 23.04163060716064 48-49 23.195781575589187 26.064621125236243 27.495477086597635 23.24412021257693 50-51 23.26379241832937 26.317799060529307 27.390816415538094 23.027592105603226 52-53 23.41726810451773 26.11933668458596 27.29014153442572 23.173253676470587 54-55 23.214545319445882 26.131734273234773 27.34809117517833 23.305629232141012 56-57 23.09727301509356 26.387580927581926 27.3193182699266 23.195827787397914 58-59 23.279723521658223 26.02039827354492 27.304457316757986 23.39542088803887 60-61 23.105793833350564 26.19125885195906 27.41232037630518 23.290626938385195 62-63 22.95488246924429 26.28040437945829 27.51083476558462 23.25387838571281 64-65 22.888169712085187 26.291388562235085 27.391745225111137 23.428696500568595 66-67 22.848486439336423 26.26805127045407 27.31262215904502 23.570840131164488 68-69 22.967835858792363 26.043434058916947 27.499528022348013 23.489202059942677 70-71 22.985977398428616 26.128463248216686 27.210304294169337 23.67525505918536 72-73 23.0048766540887 26.147604802026258 27.09672703659671 23.75079150728833 74-75 22.849846221441126 26.614513336090145 26.903776943554224 23.631863498914505 76-77 23.036698477721494 26.330741820014474 27.033265112943244 23.59929458932079 78-79 22.956033385454358 26.345299900496226 27.165257029876976 23.533409684172437 80-81 22.933056736032043 26.404549398322125 27.028407692414863 23.633986173230973 82-83 23.063135243886606 26.51145566697567 26.851342758959213 23.574066330178514 84-85 22.879694335136115 26.55152541661013 26.881489845632945 23.687290402620814 86-87 22.951049724827847 26.47181161328257 26.898179033933324 23.678959627956264 88-89 22.920651548195252 26.60649323089231 26.60162707344243 23.87122814747001 90-91 22.88905487012574 26.503326591102798 26.74593785153645 23.861680687235012 92-93 22.87267025876647 26.229456227972143 26.917003110528242 23.980870402733146 94-95 22.715025647279838 26.187059976109385 26.97475170477077 24.123162671840007 96-97 22.774849552676148 26.079501446119625 26.860914871569552 24.284734129634668 98-99 22.567558426795003 26.25968563764433 26.714439891248272 24.458316044312394 100-101 22.925931460047977 25.72929707376566 26.802505431666006 24.542266034520356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1056.0 1 730.0 2 547.5 3 716.5 4 579.5 5 498.5 6 1069.0 7 2378.0 8 3886.5 9 4529.0 10 3677.0 11 2726.0 12 2633.0 13 2700.5 14 2799.0 15 2891.5 16 2912.5 17 2993.0 18 3183.0 19 3393.5 20 3719.0 21 4255.0 22 5011.0 23 6059.5 24 7414.5 25 9288.5 26 11801.5 27 14398.0 28 16395.0 29 18650.0 30 21446.0 31 24814.0 32 30150.0 33 33579.5 34 35183.0 35 37824.5 36 42122.5 37 47634.0 38 51958.0 39 55225.5 40 58255.5 41 64002.5 42 69493.0 43 72569.5 44 76806.0 45 82271.0 46 87233.0 47 93706.5 48 97428.5 49 97358.0 50 96837.5 51 94325.0 52 93926.5 53 97990.0 54 102428.5 55 98473.5 56 89269.5 57 81711.5 58 75918.0 59 68152.0 60 56467.5 61 46880.5 62 38953.0 63 29690.0 64 21523.0 65 16102.5 66 11911.5 67 9054.5 68 7013.5 69 5312.5 70 4011.5 71 2711.0 72 1910.0 73 1424.0 74 1051.0 75 721.0 76 447.5 77 253.5 78 154.0 79 94.5 80 55.5 81 36.0 82 24.5 83 12.5 84 5.0 85 3.0 86 2.5 87 3.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011751460250180916 3 0.0068651142355008795 4 0.0 5 3.634472242323995E-4 6 4.038302491471105E-5 7 0.0 8 4.038302491471105E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.038302491471105E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014941719218443089 26-27 0.02253372790240877 28-29 2.0191512457355524E-4 30-31 1.0095756228677762E-4 32-33 0.0036950467796960608 34-35 0.016718572314690375 36-37 0.008520818257004032 38-39 0.021584726816913057 40-41 0.02364426108756332 42-43 0.01918193683448775 44-45 0.010075564716220406 46-47 0.00230183242013853 48-49 3.230641993176884E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.230641993176884E-4 68-69 2.2210663703091076E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8172361211619975E-4 82-83 9.893841104104207E-4 84-85 0.004522898790447638 86-87 8.07660498294221E-4 88-89 6.057453737206657E-5 90-91 0.0042200261035873045 92-93 8.278520107515766E-4 94-95 8.07660498294221E-5 96-97 2.4229814948826628E-4 98-99 2.2210663703091076E-4 100-101 0.002746045694200351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2476288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.71188538311613 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29585141437533 36.190909985380785 2 13.93836321609507 13.579279028213302 3 4.719543760176568 6.896936241189657 4 2.181199442574131 4.250013489775514 5 1.1736441673656273 2.85852100806386 6 0.7542501244581258 2.204456736768318 7 0.5014880863487234 1.7099901128252084 8 0.3834605010986749 1.4943267182776743 9 0.26292504355300256 1.1526817127314208 >10 1.5557808876858685 14.238186992966654 >50 0.14919292236662232 5.034715080366821 >100 0.07774572615884122 6.819164738542119 >500 0.00489528806144983 1.604625041152281 >1k 0.0014105067295698192 1.0099736827702372 >5k 1.6594196818468462E-4 0.5509204557941936 >10k+ 8.297098409234231E-5 0.4052989751818776 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10028 0.40496097384472246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8397 0.3390962602088287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5234 0.21136475240359762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.038302491471105E-5 2.0191512457355525E-5 16-17 0.0 0.0 0.0 8.07660498294221E-5 4.038302491471105E-5 18-19 0.0 0.0 0.0 1.0095756228677762E-4 4.038302491471105E-5 20-21 0.0 0.0 0.0 2.0191512457355524E-4 4.038302491471105E-5 22-23 0.0 0.0 0.0 3.634472242323995E-4 6.0574537372066576E-5 24-25 0.0 0.0 0.0 6.461283986353768E-4 8.07660498294221E-5 26-27 0.0 0.0 0.0 0.0010499586477824873 1.4134058720148869E-4 28-29 0.0 0.0 0.0 0.004058494003928461 1.615320996588442E-4 30-31 0.0 0.0 0.0 0.012155290499328026 1.615320996588442E-4 32-33 0.0 0.0 0.0 0.029419033650367 1.615320996588442E-4 34-35 0.0 0.0 0.0 0.054133444898170166 1.615320996588442E-4 36-37 0.0 0.0 0.0 0.0958894926599814 1.615320996588442E-4 38-39 0.0 0.0 0.0 0.15868509640235706 1.615320996588442E-4 40-41 0.0 0.0 0.0 0.2202490178848341 1.615320996588442E-4 42-43 0.0 0.0 0.0 0.2867800514318205 1.615320996588442E-4 44-45 0.0 0.0 0.0 0.3685152938591957 1.8172361211619975E-4 46-47 0.0 0.0 0.0 0.46412210534477416 2.0191512457355527E-4 48-49 0.0 0.0 0.0 0.5630201333609015 2.0191512457355527E-4 50-51 0.0 0.0 0.0 0.6694092124987077 2.0191512457355527E-4 52-53 0.0 0.0 0.0 0.7677418781660291 2.422981494882663E-4 54-55 0.0 0.0 0.0 0.8727175514318205 2.422981494882663E-4 56-57 0.0 0.0 0.0 0.9976222474930219 2.422981494882663E-4 58-59 0.0 0.0 0.0 1.1266863951204384 2.422981494882663E-4 60-61 0.0 0.0 0.0 1.2642915525173162 2.422981494882663E-4 62-63 0.0 0.0 0.0 1.4036735630104413 2.8268117440297737E-4 64-65 0.0 0.0 0.0 1.5430353819911093 2.8268117440297737E-4 66-67 0.0 0.0 0.0 1.7038809702264035 2.8268117440297737E-4 68-69 0.0 0.0 0.0 1.8817076204383336 2.8268117440297737E-4 70-71 0.0 0.0 0.0 2.0639965949033394 2.8268117440297737E-4 72-73 0.0 0.0 0.0 2.2536554714152794 3.0287268686033286E-4 74-75 0.0 0.0 0.0 2.446565181432854 3.230641993176884E-4 76-77 0.0 0.0 0.0 2.6512263517006103 3.230641993176884E-4 78-79 0.0 0.0 0.0 2.874928118215652 3.230641993176884E-4 80-81 0.0 0.0 0.0 3.1199117388607465 3.230641993176884E-4 82-83 0.0 0.0 0.0 3.3639059753954306 3.230641993176884E-4 84-85 0.0 0.0 0.0 3.608990553602812 3.230641993176884E-4 86-87 0.0 0.0 0.0 3.864110313501499 3.4325571177504395E-4 88-89 0.0 0.0 0.0 4.143702186498501 3.6344722423239944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6455 0.0 40.107853 1 GTATCAA 9310 0.0 36.07438 1 TATCAAC 11445 0.0 29.22039 2 ATCAACG 11425 0.0 29.143274 3 TCAACGC 11465 0.0 29.081264 4 CAACGCA 11765 0.0 28.33971 5 AACGCAG 12190 0.0 27.39062 6 CGCAGAG 14270 0.0 22.998753 8 ACGCAGA 14420 0.0 22.957138 7 TACATGG 8195 0.0 22.433197 2 GTAAGGT 1010 0.0 22.101751 4 GTACATG 8570 0.0 21.895025 1 GAGTACT 8835 0.0 21.852655 12-13 GGACCGA 885 0.0 21.466782 6 GTACTTT 8960 0.0 21.229744 14-15 GCAGAGT 15645 0.0 20.764944 9 ACATGGG 8625 0.0 20.48615 3 TAAGGTG 1145 0.0 20.325481 5 AGTACTT 8920 0.0 19.487965 12-13 CCAATAC 1170 0.0 19.486414 3 >>END_MODULE