FastQCFastQC Report
Thu 26 May 2016
SRR938221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469125
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.424279856224371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83340.3375284766870855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.21104642332810206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.039.0265851
GTATCAA91400.038.9604151
TATCAAC112700.031.6656072
ATCAACG112050.031.5948453
TCAACGC112750.031.4408364
CAACGCA114850.030.9073265
AACGCAG118550.030.1431126
CGCAGAG136600.025.82118
ACGCAGA137900.025.818987
TACATGG82200.025.3206062
GTACATG87600.024.7390331
ACATGGG84800.023.9278583
GCAGAGT148000.023.157689
GTATAGG4200.022.730641
GAGTACT84600.022.44499412-13
GTACTTT89350.021.26888814-15
AGTACTT85550.020.7512212-13
GTGGTAT34000.020.6380831
TGGTATC33600.020.5069262
AGAGTAC129000.020.3405210-11
Produced by FastQC (version 0.11.5)