FastQCFastQC Report
Thu 26 May 2016
SRR938221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469125
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99630.4035032653267858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.3419024958234192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.21671644813446056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.045.7313271
GTATCAA89150.038.6866651
TATCAAC114450.030.093192
ATCAACG114500.029.9519393
TCAACGC116400.029.5008534
CAACGCA118250.029.0794835
AACGCAG122800.028.0407036
ACCGTAT1109.487532E-525.9064468
CGCAGAG138900.024.6195168
ACGCAGA140750.024.3971547
GTACGGG1401.7788929E-523.7528671
TACATGG81000.023.4009722
GTACATG84600.022.9667191
GAGTACT89300.022.3913312-13
GCAGAGT152050.022.1467049
GTACTTT90500.021.80578214-15
ACATGGG83350.021.7696293
AGTACTT90300.021.30181112-13
AGAGTAC133850.020.42096310-11
CAGAGTA145000.020.22638310-11
Produced by FastQC (version 0.11.5)