##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938221_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469125 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50224026730117 34.0 31.0 34.0 31.0 34.0 2 32.71964643345315 34.0 31.0 34.0 31.0 34.0 3 32.781734420088085 34.0 31.0 34.0 31.0 34.0 4 36.243573735635096 37.0 35.0 37.0 35.0 37.0 5 36.201289120639906 37.0 37.0 37.0 35.0 37.0 6 36.23612737305726 37.0 37.0 37.0 35.0 37.0 7 36.20054675239204 37.0 37.0 37.0 35.0 37.0 8 36.190304257581126 37.0 37.0 37.0 35.0 37.0 9 37.898869032552014 39.0 38.0 39.0 35.0 39.0 10-11 37.88164369969118 39.0 38.0 39.0 35.0 39.0 12-13 37.82325074672202 39.0 38.0 39.0 35.0 39.0 14-15 39.15264010530046 40.5 38.5 41.0 36.0 41.0 16-17 39.10215724193793 40.0 38.0 41.0 36.0 41.0 18-19 39.11845370323495 40.0 38.5 41.0 36.0 41.0 20-21 39.03591677213588 40.0 38.5 41.0 35.5 41.0 22-23 38.88952989419329 40.0 38.0 41.0 35.0 41.0 24-25 38.85299124183668 40.0 38.0 41.0 35.0 41.0 26-27 38.82467878296968 40.0 38.0 41.0 35.0 41.0 28-29 38.77107031843264 40.0 38.0 41.0 35.0 41.0 30-31 38.58490112894244 40.0 38.0 41.0 34.5 41.0 32-33 38.41546479015845 40.0 38.0 41.0 34.0 41.0 34-35 38.22413891560775 40.0 38.0 41.0 34.0 41.0 36-37 38.099299751936414 40.0 38.0 41.0 33.5 41.0 38-39 38.10711061610894 40.0 38.0 41.0 33.5 41.0 40-41 38.05124467169544 40.0 38.0 41.0 33.0 41.0 42-43 37.89265590036956 40.0 37.5 41.0 33.0 41.0 44-45 37.69184427681871 40.0 37.0 41.0 33.0 41.0 46-47 37.388568217485954 40.0 37.0 41.0 32.5 41.0 48-49 37.05786867817547 40.0 36.5 41.0 31.5 41.0 50-51 36.763338429605625 40.0 36.0 41.0 31.0 41.0 52-53 36.34086629879006 39.0 35.0 41.0 30.0 41.0 54-55 36.108397914240875 39.0 35.0 41.0 30.0 41.0 56-57 35.853204677770464 39.0 35.0 40.0 29.0 41.0 58-59 35.510894547663646 38.0 35.0 40.0 28.5 41.0 60-61 35.140481142104996 38.0 34.0 40.0 28.0 41.0 62-63 34.75828562749962 37.0 34.0 40.0 27.5 41.0 64-65 34.34828593125095 36.5 34.0 39.5 26.0 41.0 66-67 33.91738389105453 36.0 33.5 39.0 26.0 41.0 68-69 33.453153850048096 35.5 33.0 39.0 26.0 40.0 70-71 32.93257915253379 35.0 32.5 38.0 24.5 40.0 72-73 32.508129600567 35.0 32.0 37.0 24.0 39.5 74-75 32.05018255454868 35.0 32.0 37.0 22.5 39.0 76-77 30.777568166860732 33.5 30.0 35.5 21.0 37.5 78-79 31.505185845188073 34.5 31.5 36.0 23.0 37.5 80-81 31.361867665671035 35.0 32.0 35.5 22.0 37.0 82-83 31.011425099984812 34.5 31.0 35.0 20.0 37.0 84-85 30.64475148078773 34.0 31.0 35.0 19.0 36.0 86-87 30.22198471118311 34.0 31.0 35.0 17.0 36.0 88-89 29.768378879157595 34.0 30.0 35.0 11.0 35.5 90-91 29.42842363185339 34.0 30.0 35.0 7.0 35.0 92-93 29.015602490760898 34.0 29.0 35.0 2.0 35.0 94-95 28.59486943755379 34.0 29.0 35.0 2.0 35.0 96-97 28.004460487014633 34.0 29.0 35.0 2.0 35.0 98-99 27.29935786969068 33.0 27.0 35.0 2.0 35.0 100-101 24.839862906900215 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11023053825397966 1101 2 0.14074552479662827 1101 3 0.08402114684714235 1101 4 -0.004928828435829757 1101 5 0.03265904212582171 1101 6 0.05378819866757567 1101 7 0.10998206266265953 1101 8 0.11991430663177027 1101 9 0.017483057441211258 1101 10-11 0.08166584983615621 1101 12-13 0.1251642452218249 1101 14-15 0.17583898151102773 1101 16-17 0.17484094305676479 1101 18-19 0.17512138228374852 1101 20-21 0.15960465256151934 1101 22-23 0.16120987784412932 1101 24-25 0.0930441098504673 1101 26-27 0.211468797134998 1101 28-29 0.22129751175708634 1101 30-31 0.1275696828055075 1101 32-33 0.1103188816021543 1101 34-35 0.0855331155289818 1101 36-37 0.19110067368118422 1101 38-39 0.2231585010347743 1101 40-41 0.4212267687613789 1101 42-43 0.35506760210818555 1101 44-45 0.3285098061440266 1101 46-47 0.26652139098789007 1101 48-49 0.18449077911531475 1101 50-51 0.17073770758415918 1101 52-53 0.07518140813031948 1101 54-55 0.2480612742808077 1101 56-57 0.18454479335332508 1101 58-59 0.09991360138763383 1101 60-61 0.14788124479903075 1101 62-63 0.02753996594493202 1101 64-65 0.14433013329982458 1101 66-67 0.034272754999769006 1101 68-69 0.12674227644301084 1101 70-71 0.23178690934086177 1101 72-73 -0.05643336717678693 1101 74-75 0.07084254265705425 1101 76-77 0.08814899682302979 1101 78-79 0.20231980301541697 1101 80-81 0.30362349986955905 1101 82-83 0.36552794278502176 1101 84-85 0.3730454118065403 1101 86-87 0.40515675859124656 1101 88-89 0.4205443510626985 1101 90-91 0.44664300399834644 1101 92-93 0.38445133637896944 1101 94-95 0.3039485256963168 1101 96-97 0.39193553969312944 1101 98-99 0.5830229427545 1101 100-101 0.8084191123767788 1102 1 0.12889010373140763 1102 2 0.08421533411067372 1102 3 0.10918749398349092 1102 4 0.03774634976079483 1102 5 0.02980607903785426 1102 6 6.423617822974848E-4 1102 7 0.03282119296801511 1102 8 0.006482782965072431 1102 9 0.07761042883750946 1102 10-11 0.11428220197905858 1102 12-13 0.053592933129436915 1102 14-15 0.1229657022484858 1102 16-17 0.043192711723776256 1102 18-19 -0.0020358533092661446 1102 20-21 0.03670349123408556 1102 22-23 0.10819365080710952 1102 24-25 -0.09873274935013399 1102 26-27 -0.10083226962847647 1102 28-29 -0.012153118005663543 1102 30-31 -0.013043567979600823 1102 32-33 0.028911332181273508 1102 34-35 0.1120513291437959 1102 36-37 0.13183504830061565 1102 38-39 0.14040865364771093 1102 40-41 0.20354060127336737 1102 42-43 0.014327853657611911 1102 44-45 -0.08751924965838498 1102 46-47 0.12500029135512847 1102 48-49 0.0013950735022874028 1102 50-51 0.07760795739772419 1102 52-53 0.08005902975770596 1102 54-55 0.03888296399114921 1102 56-57 0.0704061662937292 1102 58-59 0.11510559600297654 1102 60-61 0.05473177767817816 1102 62-63 -0.06583933877411141 1102 64-65 0.025397778235728197 1102 66-67 0.05792544081351281 1102 68-69 0.2429993141321205 1102 70-71 0.2410049627845794 1102 72-73 0.05318940534120742 1102 74-75 0.08030995733008695 1102 76-77 0.045782427310591345 1102 78-79 0.13570507693969347 1102 80-81 0.275729353518102 1102 82-83 0.34092372158266926 1102 84-85 0.2270803664517942 1102 86-87 0.22898227686633277 1102 88-89 0.049765273127068355 1102 90-91 -0.004464991063912294 1102 92-93 0.06027642242792197 1102 94-95 -3.3905700365721714E-4 1102 96-97 -0.12333957533198259 1102 98-99 -0.11284034775442464 1102 100-101 -0.06713455653877887 1103 1 0.1373453513192615 1103 2 0.13122726593849876 1103 3 0.1255010449414513 1103 4 0.057862416314165444 1103 5 0.09417306737417164 1103 6 0.11504319285070608 1103 7 0.09403314993234346 1103 8 0.16141402853209286 1103 9 0.06737674520154258 1103 10-11 0.11875213172464072 1103 12-13 0.12658929367687222 1103 14-15 0.18896913782457148 1103 16-17 0.1684236938533985 1103 18-19 0.12481479296391029 1103 20-21 0.20899170047962912 1103 22-23 0.31440173716987374 1103 24-25 0.2742420983194265 1103 26-27 0.21850294430164752 1103 28-29 0.16291545906049976 1103 30-31 0.27166420875411035 1103 32-33 0.21970853115880118 1103 34-35 0.19445709444249104 1103 36-37 0.28355833852745604 1103 38-39 0.2322529080405431 1103 40-41 0.17385549983767135 1103 42-43 0.1811529743671727 1103 44-45 0.2454786442537582 1103 46-47 0.13789047211861316 1103 48-49 0.29622095936846193 1103 50-51 0.17017159632389678 1103 52-53 0.14796706067903642 1103 54-55 0.18528305660333189 1103 56-57 0.3220289295885621 1103 58-59 0.28433003196221307 1103 60-61 0.21095408199347787 1103 62-63 0.23412904034688609 1103 64-65 0.13348330184257406 1103 66-67 0.22459932294010088 1103 68-69 0.13315461689441577 1103 70-71 0.2237897971973979 1103 72-73 0.011075849005031557 1103 74-75 0.10676478953631374 1103 76-77 0.0536430192847277 1103 78-79 0.17071452029206924 1103 80-81 0.24210012911265721 1103 82-83 0.2713480611916701 1103 84-85 0.2766949178539342 1103 86-87 0.4013600277445377 1103 88-89 0.15167652449570213 1103 90-91 0.2020816179782443 1103 92-93 0.3824405416558001 1103 94-95 0.20612125259487968 1103 96-97 0.15446758345956368 1103 98-99 0.37867131525778674 1103 100-101 0.5788628371226672 1104 1 0.1549187562613099 1104 2 0.051903784192880664 1104 3 0.09923484311781294 1104 4 0.05120320405146117 1104 5 0.013116996408911064 1104 6 -0.007903477480972754 1104 7 0.009152447344405346 1104 8 0.03205317111809336 1104 9 0.09859071437314526 1104 10-11 0.08898779122172584 1104 12-13 0.1552199263020384 1104 14-15 0.21956370324069496 1104 16-17 0.14605923817621402 1104 18-19 0.07668537986762658 1104 20-21 0.1341588959612139 1104 22-23 0.17192332612707162 1104 24-25 0.18512382827183416 1104 26-27 0.17571309008540936 1104 28-29 0.2012270738759412 1104 30-31 0.13172622375661547 1104 32-33 0.17317737875686845 1104 34-35 0.2437475786346397 1104 36-37 0.15372422181835788 1104 38-39 0.12760290225695314 1104 40-41 0.17580823858485672 1104 42-43 0.19626990859220683 1104 44-45 0.1321818615463215 1104 46-47 0.2246362267775126 1104 48-49 0.16281283323895224 1104 50-51 0.2319925687380433 1104 52-53 0.20329921905236858 1104 54-55 0.13091669545114115 1104 56-57 0.25725832664004855 1104 58-59 0.17574967303141875 1104 60-61 0.12335882379041863 1104 62-63 0.2521427443440274 1104 64-65 0.2128783010900861 1104 66-67 0.1864605939118391 1104 68-69 0.2255962741601536 1104 70-71 0.23080162183897102 1104 72-73 0.17878750038767777 1104 74-75 0.17743962868412666 1104 76-77 0.23738205378601407 1104 78-79 0.3627029431632458 1104 80-81 0.2791331353726143 1104 82-83 0.2009611577253736 1104 84-85 0.36970000865628805 1104 86-87 0.41111999013254774 1104 88-89 0.37051386135806297 1104 90-91 0.2660195084482595 1104 92-93 0.49194627487188924 1104 94-95 0.4122539716654252 1104 96-97 0.5481129735657717 1104 98-99 0.6682096107034248 1104 100-101 0.6694649398616015 1105 1 0.037678098585701036 1105 2 0.007931089725516927 1105 3 -0.012616109340406467 1105 4 0.03702464070328659 1105 5 -0.006945890286971235 1105 6 -0.04219340989622111 1105 7 -0.0370346018878962 1105 8 1.87846983237705E-4 1105 9 -0.05316661650418553 1105 10-11 -0.044396108517382515 1105 12-13 0.04245637890733889 1105 14-15 0.052117754554956264 1105 16-17 -0.03928160268765879 1105 18-19 0.03345518393680891 1105 20-21 0.10864574189402987 1105 22-23 0.11317640443115806 1105 24-25 0.015196708539306769 1105 26-27 0.03642666570435438 1105 28-29 -0.024366541338572745 1105 30-31 0.05350040756324148 1105 32-33 0.11390759835752107 1105 34-35 0.21199366600934155 1105 36-37 0.057233039570022015 1105 38-39 0.010276135319379875 1105 40-41 0.10734917158156065 1105 42-43 0.17322772305262646 1105 44-45 0.09455652155756411 1105 46-47 0.18370910671850993 1105 48-49 0.32127934073543685 1105 50-51 0.23729322636794592 1105 52-53 0.23621679020637032 1105 54-55 0.3400852342120615 1105 56-57 0.2637570865034391 1105 58-59 0.189161986378366 1105 60-61 0.1971890682362485 1105 62-63 0.3511168116623722 1105 64-65 0.3676081563383775 1105 66-67 0.23713724130852398 1105 68-69 0.1662698493742738 1105 70-71 0.26232569211196477 1105 72-73 0.20074666811023434 1105 74-75 0.10875684084004433 1105 76-77 0.28487225038937325 1105 78-79 0.3315356345812752 1105 80-81 0.3138788347251058 1105 82-83 0.4351643115934003 1105 84-85 0.5465750439570023 1105 86-87 0.5275174300021632 1105 88-89 0.31807634076937674 1105 90-91 0.25029856905905845 1105 92-93 0.4661833631349417 1105 94-95 0.3824879994992081 1105 96-97 0.4629962942915071 1105 98-99 0.5988715762669052 1105 100-101 0.5468547371465213 1106 1 -0.10343333076121297 1106 2 -0.0431040077967495 1106 3 -0.012657025372270425 1106 4 -0.0267142600422261 1106 5 -0.0780070428884656 1106 6 -0.08407737140516502 1106 7 0.006305626785874097 1106 8 -0.12362609563874116 1106 9 -0.10136320335751492 1106 10-11 -0.04551762944436888 1106 12-13 -0.1162202873043583 1106 14-15 -0.33649873385627416 1106 16-17 -0.2414176538033459 1106 18-19 -0.06829135280219134 1106 20-21 -0.07143961332115367 1106 22-23 0.039994021062938145 1106 24-25 0.04022857463206719 1106 26-27 0.013205880610044574 1106 28-29 0.02054387910020239 1106 30-31 0.011297785126700433 1106 32-33 -0.1506192327207927 1106 34-35 -0.04940894493322645 1106 36-37 -0.1280016451063659 1106 38-39 -0.1532133181829849 1106 40-41 -0.19383358284197527 1106 42-43 -0.2555922804517863 1106 44-45 -0.31378586692047605 1106 46-47 -0.36841963573193226 1106 48-49 -0.3957371059476955 1106 50-51 -0.40674205949300557 1106 52-53 -0.45846122092466857 1106 54-55 -0.6585650833244543 1106 56-57 -0.5666356543053652 1106 58-59 -0.4391467853629578 1106 60-61 -0.44870414904130485 1106 62-63 -0.3940862521051116 1106 64-65 -0.5131794710283799 1106 66-67 -0.5373248456152382 1106 68-69 -0.7042882234369046 1106 70-71 -0.7733194334612108 1106 72-73 -0.5953619517732669 1106 74-75 -0.6897609837138852 1106 76-77 -0.6301924703399706 1106 78-79 -0.5045567719341797 1106 80-81 -0.7452662263506618 1106 82-83 -0.883793491484294 1106 84-85 -0.6352224597902492 1106 86-87 -0.4152404948241539 1106 88-89 -0.3768941731391031 1106 90-91 -0.48347463243928246 1106 92-93 -0.3710877999017015 1106 94-95 -0.31592906542204346 1106 96-97 -0.3543254140996197 1106 98-99 -0.40274905778166925 1106 100-101 -0.3132203203467476 1107 1 0.06511739181853216 1107 2 0.11206488312161866 1107 3 0.07994468771383367 1107 4 0.08707181569617717 1107 5 0.02843881538471038 1107 6 0.004780543789522085 1107 7 0.06290099333712362 1107 8 0.04483374862751788 1107 9 0.016083564446148557 1107 10-11 0.06096671758822225 1107 12-13 0.05653280991974441 1107 14-15 0.14013447872947182 1107 16-17 0.14662646701997062 1107 18-19 0.09328789704814966 1107 20-21 0.14424683557137996 1107 22-23 0.1473088284734061 1107 24-25 0.10121786279928102 1107 26-27 0.01785973213908676 1107 28-29 0.11027156552218997 1107 30-31 0.14168596855441962 1107 32-33 0.11970959580842333 1107 34-35 0.13981509925635294 1107 36-37 0.16646113028445342 1107 38-39 0.12357519467861522 1107 40-41 0.2506254184083545 1107 42-43 0.1905073135104871 1107 44-45 0.07440271753768712 1107 46-47 0.16966510381126199 1107 48-49 0.15249850205886872 1107 50-51 0.17814082631196726 1107 52-53 0.2838229038446798 1107 54-55 0.22496598756735153 1107 56-57 0.33380976900745907 1107 58-59 0.2856418440859514 1107 60-61 0.28104250523920626 1107 62-63 0.3321643523178892 1107 64-65 0.1317194231110861 1107 66-67 0.16610042976622452 1107 68-69 0.2881762006180537 1107 70-71 0.2205947836886395 1107 72-73 0.14189778308028878 1107 74-75 0.20887173113373336 1107 76-77 0.18424776483756133 1107 78-79 0.3586756261845814 1107 80-81 0.46748678119089604 1107 82-83 0.36334320638940554 1107 84-85 0.46156608296552903 1107 86-87 0.6173969268624333 1107 88-89 0.40472875211124304 1107 90-91 0.3974429395851473 1107 92-93 0.5510486924495019 1107 94-95 0.5213708023709351 1107 96-97 0.6672195471238922 1107 98-99 0.8786467464204044 1107 100-101 0.9253220991239282 1108 1 0.011867401120170484 1108 2 0.0204450346762286 1108 3 0.0054944142458097645 1108 4 -0.0019832623755320355 1108 5 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-0.44629822247562956 2316 100-101 -0.4181287043235855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 13.0 5 41.0 6 84.0 7 186.0 8 470.0 9 937.0 10 1615.0 11 2548.0 12 3922.0 13 5616.0 14 7812.0 15 10371.0 16 13572.0 17 18822.0 18 18955.0 19 13575.0 20 11870.0 21 12356.0 22 12922.0 23 13800.0 24 15823.0 25 18622.0 26 22362.0 27 27180.0 28 33439.0 29 40762.0 30 50192.0 31 62086.0 32 79072.0 33 103827.0 34 145955.0 35 223242.0 36 371238.0 37 599362.0 38 479969.0 39 46495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51991089961019 18.301301068192174 14.513076494709665 23.665711537487976 2 16.157611722650355 19.43626770904009 39.38320388054562 25.022916687763924 3 19.825651498220104 22.868426946859877 29.123644218240614 28.182277336679405 4 13.613486559003697 15.945891763276466 35.035569280615604 35.405052397104235 5 14.452904938312106 36.60218628233587 33.60606174743016 15.33884703192186 6 34.29907355844682 34.57520376651647 16.218984458057005 14.906738216979697 7 30.177735027590746 31.791829089252268 19.414650939097857 18.61578494405913 8 27.15290526937363 34.61695010807507 18.694861361369266 19.535283261182034 9 26.45182820861435 16.677905529561045 19.462146497261564 37.40811976456303 10-11 25.958973320508278 25.924730420695592 26.001822507973472 22.11447375082266 12-13 26.51765301473194 23.004708145598137 26.902262947400395 23.57537589226953 14-15 23.33047132081203 24.58500480939604 25.04030780134663 27.044216068445298 16-17 23.053937626778442 27.73398463136975 25.88371041429529 23.328367327556514 18-19 22.998977370525996 26.255313116994888 28.103619703336204 22.642089809142917 20-21 23.4259099883562 25.28839163671341 28.613921935908472 22.671776439021922 22-23 23.342844124943046 25.09405153647547 28.54831164886346 23.014792689718018 24-25 23.28339323970941 25.4283783480016 28.413787572780226 22.874440839508765 26-27 22.749450248084603 25.66047702485626 28.514751296628653 23.07532143043048 28-29 22.79876936136675 26.104920995275855 28.364649089181203 22.731660554176187 30-31 22.900485576972727 25.55199221424436 28.33006193085849 23.217460277924424 32-33 22.68938738109341 25.569956816467453 28.410552374588033 23.3301034278511 34-35 23.335844822383038 25.70172826903719 28.13009513277473 22.83233177580504 36-37 23.412850294021993 25.29875056347971 28.15456496531233 23.133834177185967 38-39 22.84333798424146 25.70643135731511 28.225678886423122 23.224551772020302 40-41 23.30548397210257 25.636311862078013 28.07482547904655 22.983378686772863 42-43 23.03482295883867 25.596376408174955 27.898647653853498 23.470152979132877 44-45 22.977049923958763 25.766274951347786 28.185925384408208 23.07074974028525 46-47 23.362111420892592 26.139918448810185 27.482823867898087 23.015146262399135 48-49 23.200303751328914 26.044408444286944 27.505594086974128 23.249693717410015 50-51 23.24017617577077 26.293909785855313 27.425666987293067 23.040247051080847 52-53 23.457884878246343 26.102040196425857 27.250301220067836 23.18977370525996 54-55 23.18301017566952 26.137943603503267 27.3709714980003 23.308074722826913 56-57 23.11302586948818 26.359520072900317 27.313683997367487 23.213770060244013 58-59 23.22020958841695 26.031428137498104 27.322006783779678 23.42635549030527 60-61 23.08880676352959 26.21440793803473 27.367508732850705 23.329276565584976 62-63 22.94863565028097 26.295347542145496 27.539877486964002 23.21613932060953 64-65 22.855707993722476 26.313066369665368 27.42993975598643 23.40128588062573 66-67 22.822740849491215 26.233989773705257 27.334379587910696 23.608889788892824 68-69 22.93324520471577 26.05356932038701 27.55052056027283 23.46266491462439 70-71 22.957768440236926 26.148979901787072 27.2411076798461 23.652143978129907 72-73 22.95651293474409 26.18231154761302 27.09307953222295 23.768095985419937 74-75 22.806237027286993 26.635225029109506 26.939057358375944 23.61948058522756 76-77 23.080018225079733 26.30749759530198 27.028785500936564 23.58369867868172 78-79 22.958396192983344 26.36913886498253 27.132951956664808 23.53951298536931 80-81 22.92421404343644 26.45921125904926 27.00071887814509 23.615855819369212 82-83 23.044367152074987 26.514707240300613 26.899114827768877 23.541810779855528 84-85 22.899286911536976 26.60672724735865 26.843660624730536 23.650325216373837 86-87 22.915403141164056 26.480889014349867 26.915761163962898 23.687946680523176 88-89 22.867189793955347 26.618943957879814 26.69927605933276 23.814590188832078 90-91 22.888433351065693 26.491315429927376 26.796241819708676 23.82400939929825 92-93 22.822676571946047 26.339069652722568 26.91955963276 23.918694142571383 94-95 22.699397559864323 26.193428846251205 27.07131068698426 24.035862906900217 96-97 22.763837951468222 26.093661943737544 26.9197037249438 24.22279637985043 98-99 22.548595150103782 26.283926492178406 26.752695793044097 24.41478256467372 100-101 22.659504885333874 25.977137649977216 26.994724851921227 24.368632612767684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1100.0 1 746.5 2 528.0 3 672.0 4 540.0 5 493.5 6 1059.5 7 2420.5 8 3953.5 9 4538.0 10 3711.5 11 2784.0 12 2651.5 13 2698.0 14 2766.0 15 2846.0 16 2913.0 17 2966.5 18 3089.5 19 3318.5 20 3660.5 21 4191.0 22 4949.0 23 6014.5 24 7346.5 25 9139.0 26 11642.5 27 14245.5 28 16121.0 29 18220.0 30 21169.0 31 24826.0 32 30397.5 33 33747.5 34 34983.5 35 37549.5 36 42159.0 37 47494.5 38 52082.0 39 55442.0 40 58041.0 41 63905.0 42 69171.0 43 72406.0 44 76916.5 45 81971.5 46 86828.0 47 93732.0 48 97917.0 49 98104.5 50 97155.5 51 94040.5 52 93796.0 53 98147.0 54 102618.0 55 98550.0 56 89103.5 57 81179.5 58 75349.5 59 67871.5 60 55966.0 61 46186.0 62 38526.5 63 29312.0 64 21290.0 65 15863.0 66 11465.5 67 8832.5 68 6731.0 69 5017.5 70 3887.5 71 2603.5 72 1798.5 73 1402.5 74 1057.0 75 697.0 76 438.0 77 254.0 78 152.0 79 78.5 80 42.0 81 31.5 82 24.0 83 13.0 84 9.0 85 7.0 86 4.0 87 3.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01620007087531008 3 0.006561028704500582 4 0.0 5 8.100035437655039E-5 6 0.0 7 0.0 8 3.2400141750620157E-4 9 5.670024806358528E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8225079734723838E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005406773654634739 26-27 0.020715840631802762 28-29 2.0250088594137598E-5 30-31 2.0250088594137598E-5 32-33 5.0625221485344E-4 34-35 0.019035083278489344 36-37 0.0037260163013213186 38-39 0.01747582645674075 40-41 0.014863565028097 42-43 0.007836784285931252 44-45 0.002835012403179264 46-47 4.0500177188275196E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.632511517237888E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.0500177188275196E-5 84-85 0.0030982635549030527 86-87 3.442515061003392E-4 88-89 0.0 90-91 1.8225079734723838E-4 92-93 1.6200070875310079E-4 94-95 0.0 96-97 2.0250088594137598E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2469125.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.49261680888459 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16018214313442 35.96221295144103 2 13.981743864175519 13.560226950508733 3 4.794583343551683 6.975056785113372 4 2.168538293296426 4.206323859688648 5 1.2157982466629498 2.9478619246170084 6 0.7529298664275742 2.190692369798221 7 0.5051583190551583 1.7147514155633439 8 0.36627914402799006 1.4209467341148456 9 0.2570214900535149 1.1217280165932273 >10 1.5596050589055646 14.249140622142036 >50 0.1500465604385086 5.033833224402703 >100 0.08159365106913664 7.03304172573483 >500 0.004848219406223219 1.594524249662407 >1k 0.0014210298259616256 1.0267103419094346 >5k 2.5076996928734567E-4 0.962948828710195 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9963 0.4035032653267858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8442 0.3419024958234192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5351 0.21671644813446056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.0500177188275196E-5 0.0 5 0.0 0.0 0.0 4.0500177188275196E-5 0.0 6 0.0 0.0 0.0 4.0500177188275196E-5 0.0 7 0.0 0.0 0.0 4.0500177188275196E-5 0.0 8 0.0 0.0 0.0 4.0500177188275196E-5 0.0 9 0.0 0.0 0.0 4.0500177188275196E-5 0.0 10-11 0.0 0.0 0.0 4.0500177188275196E-5 0.0 12-13 0.0 0.0 0.0 4.0500177188275196E-5 1.417506201589632E-4 14-15 0.0 0.0 0.0 4.0500177188275196E-5 1.8225079734723838E-4 16-17 0.0 0.0 0.0 4.0500177188275196E-5 2.430010631296512E-4 18-19 0.0 0.0 0.0 4.0500177188275196E-5 2.430010631296512E-4 20-21 0.0 0.0 0.0 1.01250442970688E-4 2.430010631296512E-4 22-23 0.0 0.0 0.0 2.02500885941376E-4 2.835012403179264E-4 24-25 0.0 0.0 0.0 3.2400141750620157E-4 3.2400141750620157E-4 26-27 0.0 0.0 0.0 6.07502657824128E-4 3.2400141750620157E-4 28-29 0.0 0.0 0.0 0.0034020148838151167 3.2400141750620157E-4 30-31 0.0 0.0 0.0 0.01198805244772946 3.2400141750620157E-4 32-33 0.0 0.0 0.0 0.029139877486964005 3.2400141750620157E-4 34-35 0.0 0.0 0.0 0.053804485394623604 3.2400141750620157E-4 36-37 0.0 0.0 0.0 0.09711942489748393 3.2400141750620157E-4 38-39 0.0 0.0 0.0 0.16023895104541083 3.2400141750620157E-4 40-41 0.0 0.0 0.0 0.22564673720447526 3.2400141750620157E-4 42-43 0.0 0.0 0.0 0.29322128284311244 3.2400141750620157E-4 44-45 0.0 0.0 0.0 0.37432288766263355 3.2400141750620157E-4 46-47 0.0 0.0 0.0 0.4669265428036248 3.2400141750620157E-4 48-49 0.0 0.0 0.0 0.5663342277122463 3.2400141750620157E-4 50-51 0.0 0.0 0.0 0.6689819267959297 3.2400141750620157E-4 52-53 0.0 0.0 0.0 0.7710828734875715 3.2400141750620157E-4 54-55 0.0 0.0 0.0 0.881223105351086 3.2400141750620157E-4 56-57 0.0 0.0 0.0 1.009264415531818 3.2400141750620157E-4 58-59 0.0 0.0 0.0 1.139674986078064 3.2400141750620157E-4 60-61 0.0 0.0 0.0 1.2791373462258897 3.2400141750620157E-4 62-63 0.0 0.0 0.0 1.4181339543360503 3.2400141750620157E-4 64-65 0.0 0.0 0.0 1.5568065610287045 3.2400141750620157E-4 66-67 0.0 0.0 0.0 1.7159317572014379 3.442515061003392E-4 68-69 0.0 0.0 0.0 1.8957727940059739 3.645015946944768E-4 70-71 0.0 0.0 0.0 2.0841391181086415 3.645015946944768E-4 72-73 0.0 0.0 0.0 2.2757252062977775 4.252518604768896E-4 74-75 0.0 0.0 0.0 2.468708550599909 4.860021262593024E-4 76-77 0.0 0.0 0.0 2.6768389611704553 4.860021262593024E-4 78-79 0.0 0.0 0.0 2.901533944211006 4.860021262593024E-4 80-81 0.0 0.0 0.0 3.1422467473295197 4.860021262593024E-4 82-83 0.0 0.0 0.0 3.38867007543158 4.860021262593024E-4 84-85 0.0 0.0 0.0 3.6389611704551204 4.860021262593024E-4 86-87 0.0 0.0 0.0 3.8976560522452286 5.0625221485344E-4 88-89 0.0 0.0 0.0 4.182048296461296 5.265023034475776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5890 0.0 45.731327 1 GTATCAA 8915 0.0 38.686665 1 TATCAAC 11445 0.0 30.09319 2 ATCAACG 11450 0.0 29.951939 3 TCAACGC 11640 0.0 29.500853 4 CAACGCA 11825 0.0 29.079483 5 AACGCAG 12280 0.0 28.040703 6 ACCGTAT 110 9.487532E-5 25.906446 8 CGCAGAG 13890 0.0 24.619516 8 ACGCAGA 14075 0.0 24.397154 7 GTACGGG 140 1.7788929E-5 23.752867 1 TACATGG 8100 0.0 23.400972 2 GTACATG 8460 0.0 22.966719 1 GAGTACT 8930 0.0 22.39133 12-13 GCAGAGT 15205 0.0 22.146704 9 GTACTTT 9050 0.0 21.805782 14-15 ACATGGG 8335 0.0 21.769629 3 AGTACTT 9030 0.0 21.301811 12-13 AGAGTAC 13385 0.0 20.420963 10-11 CAGAGTA 14500 0.0 20.226383 10-11 >>END_MODULE