FastQCFastQC Report
Thu 26 May 2016
SRR938220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616479
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106120.40558322845319994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84770.32398501956254955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52830.20191257029007303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61150.042.762041
GTATCAA92550.039.5245861
TATCAAC115700.031.2032342
ATCAACG116550.030.8940433
TCAACGC118650.030.3472464
CAACGCA120250.029.9039025
AACGCAG123750.029.0904396
CGCAGAG144100.024.7408088
ACGCAGA145550.024.5293437
TACATGG88800.023.5148752
GATCGGA4800.022.779537
GTACATG94100.022.6256161
GCAGAGT157250.022.1575419
GAGTACT88350.021.87151512-13
ACATGGG91950.021.46783
ATCGGAC4550.020.8946538
GTACTTT91750.020.7491614-15
AGTACTT89800.020.35377112-13
AGAGTAC138900.019.77977810-11
CAGAGTA150700.019.44534310-11
Produced by FastQC (version 0.11.5)