##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938220_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2616479 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02196081069254 33.0 31.0 34.0 30.0 34.0 2 32.13801524873695 34.0 31.0 34.0 30.0 34.0 3 32.16842176069443 34.0 31.0 34.0 30.0 34.0 4 35.6739415833263 37.0 35.0 37.0 35.0 37.0 5 35.66988766200684 37.0 35.0 37.0 35.0 37.0 6 35.69968304733193 37.0 36.0 37.0 35.0 37.0 7 35.63103621317045 37.0 35.0 37.0 33.0 37.0 8 35.62459664304586 37.0 35.0 37.0 33.0 37.0 9 37.2682284092477 39.0 37.0 39.0 34.0 39.0 10-11 37.17729475375113 39.0 37.0 39.0 34.0 39.0 12-13 37.02699008858852 39.0 37.0 39.0 33.0 39.0 14-15 38.23002057345005 40.0 38.0 41.0 33.0 41.0 16-17 38.0537193304437 40.0 38.0 41.0 33.0 41.0 18-19 38.212896224276975 40.0 38.0 41.0 33.5 41.0 20-21 38.207809426332105 40.0 38.0 41.0 34.0 41.0 22-23 38.09468354227188 40.0 38.0 41.0 33.0 41.0 24-25 37.97607758365345 40.0 38.0 41.0 33.0 41.0 26-27 37.79665879221656 40.0 38.0 41.0 33.0 41.0 28-29 37.61888629719558 40.0 38.0 41.0 32.0 41.0 30-31 37.41125497280888 40.0 37.0 41.0 32.0 41.0 32-33 37.128352836005945 40.0 36.5 41.0 31.0 41.0 34-35 36.894279487815496 39.5 36.0 41.0 30.5 41.0 36-37 36.63752508619408 39.0 36.0 41.0 30.0 41.0 38-39 36.34858621070531 39.0 35.0 41.0 30.0 41.0 40-41 36.25348206501944 39.0 35.0 41.0 30.0 41.0 42-43 36.039439070598306 39.0 35.0 41.0 29.0 41.0 44-45 35.721873747123524 39.0 35.0 40.5 27.5 41.0 46-47 35.34804043907863 39.0 35.0 40.0 26.5 41.0 48-49 34.95216491322881 38.0 34.5 40.0 24.5 41.0 50-51 34.220545244200316 37.5 33.5 39.5 23.5 40.5 52-53 34.28794536474399 38.0 34.0 40.0 24.0 40.5 54-55 34.368775938962244 38.0 34.0 40.0 23.5 41.0 56-57 34.041235377772956 38.0 33.5 40.0 22.5 41.0 58-59 33.62702719953036 37.5 33.0 40.0 21.0 41.0 60-61 33.114213223190404 37.0 32.5 40.0 18.5 41.0 62-63 32.555774382290096 36.0 32.0 39.0 15.0 41.0 64-65 31.996868501524375 35.5 31.0 39.0 10.0 40.0 66-67 31.43017754012167 35.0 30.5 38.5 7.5 40.0 68-69 30.744367525976706 35.0 30.0 38.0 2.0 40.0 70-71 30.156485872808457 34.5 29.0 37.0 2.0 39.5 72-73 29.470694203928257 34.0 28.5 36.5 2.0 39.0 74-75 28.79581166139686 34.0 27.5 36.0 2.0 39.0 76-77 28.06025540430479 33.5 26.0 35.5 2.0 37.5 78-79 27.315583270494432 33.0 25.5 35.0 2.0 37.0 80-81 26.61236035144941 32.5 24.5 35.0 2.0 36.5 82-83 25.902273819128684 32.0 21.5 35.0 2.0 36.0 84-85 25.196236048521698 31.5 19.0 34.5 2.0 35.5 86-87 24.46352827597699 31.0 15.0 34.0 2.0 35.0 88-89 23.602139554722203 30.5 6.0 34.0 2.0 35.0 90-91 22.721081269905092 30.0 2.0 34.0 2.0 35.0 92-93 21.78334586289437 29.0 2.0 34.0 2.0 35.0 94-95 20.717842184095495 29.0 2.0 34.0 2.0 35.0 96-97 19.465719770730054 26.5 2.0 33.5 2.0 35.0 98-99 17.928778331490527 24.0 2.0 33.0 2.0 35.0 100-101 14.021065332456327 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26341985014166625 1101 2 0.22094302198333793 1101 3 0.19556937021529563 1101 4 0.14897734333044355 1101 5 0.18022201006905192 1101 6 0.1732625929606968 1101 7 0.1784644862344109 1101 8 0.1753225018130138 1101 9 0.1345248870310769 1101 10-11 0.242168322228558 1101 12-13 0.19672584428068518 1101 14-15 0.23785138318677923 1101 16-17 0.2827469173028945 1101 18-19 0.33425850198146634 1101 20-21 0.25557076642416376 1101 22-23 0.33020813584871433 1101 24-25 0.317964780351339 1101 26-27 0.4090672101862367 1101 28-29 0.28091032601025034 1101 30-31 0.30889219983249205 1101 32-33 0.3150451547931823 1101 34-35 0.2593992437398569 1101 36-37 0.21456912353373525 1101 38-39 0.08865012430630514 1101 40-41 0.18195589083723718 1101 42-43 0.14823508306448474 1101 44-45 0.3972621848473139 1101 46-47 0.34322116202550745 1101 48-49 0.4009727773152676 1101 50-51 0.31638044529429976 1101 52-53 0.2611199005196241 1101 54-55 0.20776392058815674 1101 56-57 0.17654627898335917 1101 58-59 0.28109244825711244 1101 60-61 0.18119146851193335 1101 62-63 0.21495664231606781 1101 64-65 0.1492461067151183 1101 66-67 0.048219143403784415 1101 68-69 0.14202097949853965 1101 70-71 0.11337121042229725 1101 72-73 0.0062657485723640605 1101 74-75 0.07113754784697335 1101 76-77 0.01883315947452502 1101 78-79 -0.09068953319596673 1101 80-81 0.030023799807057827 1101 82-83 -0.14448258619075816 1101 84-85 -0.18838859958686882 1101 86-87 -0.004576205881196671 1101 88-89 0.0925004547116508 1101 90-91 0.09614436768587709 1101 92-93 -0.16468103440537263 1101 94-95 -0.30159900351423374 1101 96-97 -0.3547301213363241 1101 98-99 -0.6232516969313373 1101 100-101 -1.035119558239053 1102 1 0.24071020570427493 1102 2 0.1561722778932264 1102 3 0.23404090934128874 1102 4 0.2719346652406429 1102 5 0.23639235223416222 1102 6 0.21081525747671037 1102 7 0.2093999314368702 1102 8 0.23482679430160402 1102 9 0.31518841586214563 1102 10-11 0.28610022414474656 1102 12-13 0.20854781618118778 1102 14-15 0.2765553953855573 1102 16-17 0.24345015385691227 1102 18-19 0.27495252775402435 1102 20-21 0.24198266023135062 1102 22-23 0.37284325669064344 1102 24-25 0.3064766441904254 1102 26-27 0.26716718903227843 1102 28-29 0.26292285972436247 1102 30-31 0.3137917626509079 1102 32-33 0.19670644483144173 1102 34-35 0.10996753573484597 1102 36-37 0.16344818465101696 1102 38-39 0.10063736142443247 1102 40-41 0.19658076082859566 1102 42-43 0.3414466489854746 1102 44-45 0.34460648297694974 1102 46-47 0.3214491222560909 1102 48-49 0.24323704300884685 1102 50-51 0.26097803550707255 1102 52-53 0.2116350872913486 1102 54-55 0.08567919902699117 1102 56-57 0.18710011578065888 1102 58-59 0.054922590869978194 1102 60-61 0.1624096274133322 1102 62-63 0.12635685738120372 1102 64-65 -0.03372893162641333 1102 66-67 -0.06611463945094087 1102 68-69 -0.09277021340783875 1102 70-71 -0.3324361211834663 1102 72-73 -0.2996401692445474 1102 74-75 -0.37925754619995544 1102 76-77 -0.7539789493977942 1102 78-79 -0.744887490077172 1102 80-81 -0.7659992579961035 1102 82-83 -0.7725895722820368 1102 84-85 -0.8844438290190624 1102 86-87 -0.9561138334659454 1102 88-89 -0.9965080861947975 1102 90-91 -0.9070265209562187 1102 92-93 -1.0168358724895334 1102 94-95 -1.1319840053652044 1102 96-97 -1.1920243110519806 1102 98-99 -1.3652925240506981 1102 100-101 -1.454029424792898 1103 1 0.22684222878827853 1103 2 0.23184761160613476 1103 3 0.1739671375243006 1103 4 0.09943513971398232 1103 5 0.12438870406140978 1103 6 0.22192144812361647 1103 7 0.08661027425264223 1103 8 0.14863678003225544 1103 9 0.09168476417477223 1103 10-11 0.18338447936219637 1103 12-13 0.11648013168135662 1103 14-15 0.15806191851525853 1103 16-17 0.20899943582060132 1103 18-19 0.14552287984838586 1103 20-21 0.20499720020308843 1103 22-23 0.23579769670666906 1103 24-25 0.10930353874487508 1103 26-27 0.13417204974800256 1103 28-29 0.1979421549732976 1103 30-31 0.19324979109119056 1103 32-33 0.17323038288982673 1103 34-35 0.13169955671008182 1103 36-37 0.04021419197547971 1103 38-39 0.06872077032203805 1103 40-41 0.1864946878510878 1103 42-43 0.30033907006473726 1103 44-45 0.4416039454232674 1103 46-47 0.5497738623501363 1103 48-49 0.4042516778752585 1103 50-51 0.4560562533149408 1103 52-53 0.32562273692618504 1103 54-55 0.27875979623076574 1103 56-57 0.4165841523889142 1103 58-59 0.37324824476453244 1103 60-61 0.30230220313575984 1103 62-63 0.4139284677085584 1103 64-65 0.17574419209514502 1103 66-67 0.11303832020827898 1103 68-69 0.17278706431018875 1103 70-71 0.05361111175833955 1103 72-73 0.056980315350884325 1103 74-75 -0.09406172929745438 1103 76-77 -0.14543148800586536 1103 78-79 -0.32731870901674043 1103 80-81 -0.4111986422351528 1103 82-83 -0.441248499761425 1103 84-85 -0.5136232361734194 1103 86-87 -0.7323021255728186 1103 88-89 -0.5491954504427063 1103 90-91 -0.4006868542825224 1103 92-93 -0.4774240964128289 1103 94-95 -0.5570889166914093 1103 96-97 -0.7209013732536782 1103 98-99 -0.8326371603932436 1103 100-101 -0.8136113259196858 1104 1 0.259285403237385 1104 2 0.2359075059250486 1104 3 0.15615196647238605 1104 4 0.08572588404188508 1104 5 0.19108911950608132 1104 6 0.2100204017720344 1104 7 0.23516374863355338 1104 8 0.28177152551486984 1104 9 0.2387479929287366 1104 10-11 0.18995001126106814 1104 12-13 0.26438052537061907 1104 14-15 0.2872455467320236 1104 16-17 0.22235265583056218 1104 18-19 0.2567562024434693 1104 20-21 0.31337721702169574 1104 22-23 0.29277428035413067 1104 24-25 0.31774505643578976 1104 26-27 0.3537238882893803 1104 28-29 0.23776041362003042 1104 30-31 0.2843631242292801 1104 32-33 0.25176825194735386 1104 34-35 0.24590751333314387 1104 36-37 0.23299139565214233 1104 38-39 0.090218881366674 1104 40-41 0.2625741955094085 1104 42-43 0.179799870580176 1104 44-45 0.0773828100418612 1104 46-47 0.09015901679263294 1104 48-49 0.20315637662557862 1104 50-51 0.09727731757288893 1104 52-53 0.18708177190637088 1104 54-55 0.09972699158180376 1104 56-57 0.1585503394603407 1104 58-59 0.15777889269923406 1104 60-61 0.15762286533699665 1104 62-63 0.24946300233104068 1104 64-65 0.26127977265083047 1104 66-67 0.14845110820599317 1104 68-69 0.21805463494206023 1104 70-71 -0.04544336462658194 1104 72-73 0.016731968971278377 1104 74-75 -0.04728341274786274 1104 76-77 -0.07468205594759425 1104 78-79 -0.11134270690813963 1104 80-81 -0.09292786268021658 1104 82-83 -0.20537382766148227 1104 84-85 0.03383953906390147 1104 86-87 -0.0312545188771729 1104 88-89 -0.07334939507518712 1104 90-91 0.008970456163002183 1104 92-93 -0.1910463304424752 1104 94-95 -0.5633841794507504 1104 96-97 -0.7419685598188757 1104 98-99 -0.8479538062109775 1104 100-101 -0.7995339017603005 1105 1 0.14399023364183705 1105 2 0.16194823709383854 1105 3 0.1807310659629522 1105 4 0.11504087410028063 1105 5 0.08003177977769838 1105 6 0.10594924576138709 1105 7 0.13327386318751167 1105 8 0.18248175223052954 1105 9 0.1819690730537573 1105 10-11 0.18244890337013686 1105 12-13 0.16650689160601928 1105 14-15 0.18624236620266288 1105 16-17 0.24771022027451295 1105 18-19 0.23528547233354402 1105 20-21 0.2574249994750559 1105 22-23 0.1793399753448952 1105 24-25 0.11059455190422085 1105 26-27 0.08425069532812302 1105 28-29 0.17138813873758352 1105 30-31 0.07141982079266285 1105 32-33 0.11320031372257233 1105 34-35 0.016325060756756216 1105 36-37 0.07170796773957022 1105 38-39 0.034012535210564465 1105 40-41 -0.02054664499743808 1105 42-43 0.09959837169198948 1105 44-45 0.19151753742224997 1105 46-47 0.3652014696043224 1105 48-49 0.20062254183270767 1105 50-51 0.12930654236297556 1105 52-53 0.20931920175628704 1105 54-55 0.17915159421171722 1105 56-57 0.18172271160188558 1105 58-59 0.1723661886218011 1105 60-61 0.10298325343216419 1105 62-63 0.22756990106000075 1105 64-65 0.2361582968224596 1105 66-67 0.12558933769824066 1105 68-69 0.12774132730918097 1105 70-71 -0.15352214449551482 1105 72-73 -0.2681748309298939 1105 74-75 -0.4877059407784863 1105 76-77 -0.5296852129465215 1105 78-79 -0.6083064975030261 1105 80-81 -0.46433486455088513 1105 82-83 -0.565862319321905 1105 84-85 -0.547372199481682 1105 86-87 -0.43352245984717186 1105 88-89 -0.5260340206864846 1105 90-91 -0.6594140623848546 1105 92-93 -0.8102538256677612 1105 94-95 -1.106885663828507 1105 96-97 -0.9827801242393015 1105 98-99 -1.0168422680661955 1105 100-101 -1.3055498475461977 1106 1 0.14578661078068222 1106 2 0.16658140899200902 1106 3 0.2664282219010161 1106 4 0.2961690515439983 1106 5 0.23130500662957587 1106 6 0.22735108435736606 1106 7 0.29007516297416913 1106 8 0.1550524817686707 1106 9 0.4454923392695136 1106 10-11 0.31926788662275385 1106 12-13 0.36483102849889093 1106 14-15 0.2680457855828422 1106 16-17 -0.3091330388124902 1106 18-19 0.1769879125480287 1106 20-21 0.34741195316064477 1106 22-23 0.34823879027583615 1106 24-25 0.4092076456283209 1106 26-27 0.3025960800646246 1106 28-29 0.2880213244779952 1106 30-31 0.1595866924975624 1106 32-33 0.2753320019052765 1106 34-35 0.23318188524230266 1106 36-37 0.13045635209021356 1106 38-39 0.28549209800331 1106 40-41 0.06481102992093923 1106 42-43 -0.07468248050572868 1106 44-45 0.00934850978794799 1106 46-47 0.16604394314401105 1106 48-49 0.1252970328274614 1106 50-51 0.053960356506635776 1106 52-53 0.01247393554399423 1106 54-55 0.07304958725945454 1106 56-57 -0.10873790635046987 1106 58-59 -0.10482084258035229 1106 60-61 0.17710621754095968 1106 62-63 -0.08668648497248199 1106 64-65 -0.1270661499636283 1106 66-67 -0.23473887919587 1106 68-69 -0.3609214427659637 1106 70-71 -0.22102972744948346 1106 72-73 -0.20840956077255868 1106 74-75 -0.16982513633650953 1106 76-77 -0.6031509300352909 1106 78-79 -0.32135229283507627 1106 80-81 -0.19039574273741522 1106 82-83 -0.2347342283922096 1106 84-85 -0.200620627954482 1106 86-87 -0.3819203066862116 1106 88-89 -0.49535095788249706 1106 90-91 -0.5732957561909693 1106 92-93 -0.47101236330395224 1106 94-95 -0.6650266332354171 1106 96-97 -0.5381943447220117 1106 98-99 -0.5236734151902063 1106 100-101 -0.9495237133906347 1107 1 -0.024146428579108203 1107 2 -0.013100063353640223 1107 3 -0.06728712685936244 1107 4 0.07818185046872372 1107 5 0.012923588160305144 1107 6 0.013673993456464473 1107 7 0.10187296324111372 1107 8 0.107432897799427 1107 9 -0.08977416107495628 1107 10-11 0.10041106074857709 1107 12-13 0.06837485514835606 1107 14-15 0.04233286219805876 1107 16-17 0.0734821618347894 1107 18-19 0.018186352198036104 1107 20-21 0.08715009894974202 1107 22-23 0.17894166111707221 1107 24-25 0.10966108949270392 1107 26-27 0.004577116330750641 1107 28-29 -0.10267580205265148 1107 30-31 -0.09907764265480523 1107 32-33 -0.025791893112753428 1107 34-35 0.026740091440522917 1107 36-37 -0.0918770957538797 1107 38-39 -0.0901773676068558 1107 40-41 0.06305066153417016 1107 42-43 0.053636219853387956 1107 44-45 -0.05645294356018127 1107 46-47 0.01321149116813558 1107 48-49 -0.191950822911501 1107 50-51 -0.1960832889905575 1107 52-53 -0.1721845314008803 1107 54-55 -0.35246819851530375 1107 56-57 -0.420315601905763 1107 58-59 -0.3810949625838802 1107 60-61 -0.3750764327638123 1107 62-63 -0.31804469530059976 1107 64-65 -0.35923719901365914 1107 66-67 -0.42272613658791514 1107 68-69 -0.3282647554310394 1107 70-71 -0.4020266656943896 1107 72-73 -0.374625693274659 1107 74-75 -0.5920878127722595 1107 76-77 -0.6939345028087622 1107 78-79 -0.6940111577517918 1107 80-81 -0.6306328551137774 1107 82-83 -0.7299900097780174 1107 84-85 -0.7054779494162382 1107 86-87 -0.7399152231590556 1107 88-89 -0.8675794688107672 1107 90-91 -0.85606038400282 1107 92-93 -0.9599386089839896 1107 94-95 -0.8438478669105933 1107 96-97 -0.9095673100440216 1107 98-99 -0.8724661713517321 1107 100-101 -0.9512897134544627 1108 1 -0.005506421668670214 1108 2 0.06763162511274601 1108 3 0.03361138209163528 1108 4 0.1225283969906954 1108 5 0.12763616076919249 1108 6 0.07122160503509178 1108 7 0.14460404249751235 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-0.26217283934566993 2316 98-99 -0.3947874735456445 2316 100-101 0.01100750363112546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8357.0 3 2452.0 4 2374.0 5 2649.0 6 3078.0 7 3743.0 8 4864.0 9 6130.0 10 7632.0 11 9617.0 12 12031.0 13 14613.0 14 17033.0 15 20403.0 16 24133.0 17 30148.0 18 29875.0 19 26271.0 20 27490.0 21 29774.0 22 32592.0 23 35868.0 24 40221.0 25 44777.0 26 51087.0 27 57932.0 28 67224.0 29 77490.0 30 91005.0 31 108598.0 32 132958.0 33 165600.0 34 213341.0 35 282431.0 36 359633.0 37 379855.0 38 182735.0 39 10465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25496262268528 17.90050143894337 14.097702704616447 23.746833233754906 2 15.928242492469813 19.118357289339276 39.88511518116996 25.06828503702095 3 19.75687159836312 22.92701822640055 28.830110593987662 28.485999581248667 4 13.380509949834662 15.640456627400019 35.170879383918084 35.808154038847235 5 14.505280990349393 36.500485172559834 33.48291863269412 15.51131520439665 6 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28.405857175280847 23.211418567649815 28-29 22.933550688658585 26.090139319788808 28.229175934403084 22.747134057149527 30-31 22.9377903631567 25.617987672381602 28.179514821910594 23.264707142551096 32-33 22.800614087350557 25.626131467805756 28.243904566968542 23.32934987787514 34-35 23.438045715697832 25.70554524613129 27.973867235272536 22.882541802898345 36-37 23.453567087719097 25.34027978996149 28.00166011133074 23.204493010988674 38-39 22.922929736104113 25.714420093621975 28.079346190995864 23.28330397927805 40-41 23.353080799795336 25.687767469375938 27.957712735548 23.001438995280722 42-43 23.05283673521287 25.610245543751002 27.789750593844666 23.54716712719146 44-45 23.012986635610282 25.821576947517418 27.974048904627796 23.19138751224451 46-47 23.426993789959923 26.161246902627795 27.338152742512772 23.073606564899507 48-49 23.26370746697699 26.052470132417042 27.387652559298797 23.29616984130717 50-51 23.32846544506762 26.272773055836936 27.272200610531844 23.1265608885636 52-53 23.456230367953275 26.16515414806403 27.1387832427071 23.239832241275597 54-55 23.286625445952303 26.13249897712977 27.249836531953704 23.33103904496423 56-57 23.19461894688391 26.354682109917515 27.245452152625777 23.205246790572797 58-59 23.340722176931514 25.98586955357976 27.188490840485102 23.484917429003627 60-61 23.244856254217556 26.12795372301481 27.304434431698056 23.322755591069583 62-63 23.055199139937407 26.192790991444685 27.505330938832195 23.24667892978571 64-65 23.011184034071132 26.28197346594942 27.313604689330155 23.393237810649293 66-67 22.962961546916965 26.17870217260397 27.25351506867646 23.6048212118026 68-69 23.10048697396744 25.985597869682504 27.439999250426844 23.473915905923214 70-71 23.120264885366872 26.090397981927744 27.17661841357098 23.612718719134403 72-73 23.131072021596633 26.102731220766113 27.038024489312097 23.728172268325164 74-75 23.00447691515349 26.558040266619052 26.87869659025693 23.55878622797053 76-77 23.269166261302026 26.241978319601905 26.856552524266924 23.63230289482914 78-79 23.302292154074348 26.258257259507317 26.936384696545712 23.503065889872623 80-81 23.24176604514222 26.394817339784645 26.814115531408273 23.54930108366486 82-83 23.367154979263187 26.44900624226121 26.63087627524056 23.55296250323505 84-85 23.242310916611906 26.50082245487202 26.640389395970644 23.616477232545435 86-87 23.336367358375952 26.303984078822978 26.690296637431203 23.669351925369863 88-89 23.33513394133332 26.391189577252298 26.469473001874867 23.804203479539517 90-91 23.37334324255302 26.302435888974657 26.473532531604764 23.85068833686756 92-93 23.417680607419708 26.09973573983857 26.619076378415656 23.863507274326068 94-95 23.319813049082267 25.971720231470325 26.681296111436207 24.027170608011197 96-97 23.405252503001652 25.798217499891486 26.578293149733028 24.218236847373834 98-99 23.24914831596187 25.933755061444764 26.36719713450241 24.449899488090953 100-101 24.294424295419255 24.803002486446047 26.26990041778799 24.632672800346704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1048.0 1 726.0 2 553.0 3 718.5 4 587.5 5 569.5 6 1084.5 7 2343.5 8 3934.5 9 4490.5 10 3645.5 11 2831.0 12 2770.0 13 2885.0 14 2988.5 15 3101.5 16 3155.5 17 3277.5 18 3472.5 19 3766.5 20 4079.5 21 4584.5 22 5462.5 23 6566.0 24 8046.5 25 10072.5 26 12615.0 27 15350.5 28 17449.5 29 19496.0 30 22608.0 31 26616.5 32 31994.5 33 35240.0 34 36937.5 35 39958.0 36 44472.5 37 49843.5 38 54486.0 39 58133.0 40 61238.0 41 66763.0 42 71840.0 43 75624.5 44 80062.0 45 85414.5 46 91031.0 47 97226.5 48 100966.5 49 101418.5 50 100876.0 51 98598.5 52 98631.5 53 102231.0 54 105904.5 55 102294.0 56 93813.5 57 86169.0 58 79825.5 59 72285.5 60 61075.0 61 51351.5 62 42882.5 63 33312.5 64 25172.0 65 19275.0 66 14523.0 67 11373.5 68 8900.0 69 6679.0 70 5166.0 71 3775.0 72 2722.0 73 2006.0 74 1495.5 75 1067.0 76 753.0 77 486.0 78 287.5 79 178.5 80 113.5 81 71.0 82 44.0 83 30.0 84 21.5 85 16.5 86 9.0 87 8.5 88 7.0 89 5.0 90 4.5 91 2.5 92 2.5 93 2.5 94 2.0 95 1.5 96 2.5 97 2.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14515690743170498 2 0.05113742552491344 3 0.15096624127310024 4 0.12688808127258044 5 0.19251062209939387 6 0.19530063111532714 7 0.163272856384477 8 0.06917693587450922 9 0.11974107187560075 10-11 0.09979059644659866 12-13 0.14739273657461038 14-15 0.1347803670505286 16-17 0.1307673403837753 18-19 0.12761424800275484 20-21 0.12906658146310365 22-23 0.18949129727393188 24-25 0.2107412289569303 26-27 0.14339881955865116 28-29 0.12407896260585313 30-31 0.09596866628778598 32-33 0.12115518603436144 34-35 0.09042686755750763 36-37 0.08394869593832016 38-39 0.04895892533439022 40-41 0.05580018031866489 42-43 0.05837998317586344 44-45 0.0390219069214773 46-47 0.051940030858264104 48-49 0.04370377136602281 50-51 0.05281907479479102 52-53 0.02669618215930646 54-55 0.04926467974709524 56-57 0.027078375175187724 58-59 0.04872960952486146 60-61 0.03531463466742902 62-63 0.05046858774712123 64-65 0.03858238495321384 66-67 0.03646121371507281 68-69 0.0633484923823199 70-71 0.050067285080445896 72-73 0.04802255244548112 74-75 0.04058889828659049 76-77 0.019931365778208043 78-79 0.022893361651287858 80-81 0.025970015429132052 82-83 0.022931580952875983 84-85 0.020256229841707118 86-87 0.022625826540170972 88-89 0.06197259752514735 90-91 0.10516040831973046 92-93 0.07682079619213454 94-95 0.07219626069997123 96-97 0.0637689046997893 98-99 0.05220756596938099 100-101 0.12656321720908137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2616479.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.314670533551705 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96939343769913 38.22375001451597 2 13.007694639560194 13.089557403810357 3 4.444325394803374 6.708443039502867 4 2.0366027424480624 4.0988398397600845 5 1.1432845371052907 2.8761992405278436 6 0.6848330143530293 2.0674288492603052 7 0.46302823643770546 1.6307979214866244 8 0.32711596812735677 1.3166985730073406 9 0.2552566865739076 1.155884047780701 >10 1.4470458273985691 13.672601238408951 >50 0.13821252024168096 4.805835885332434 >100 0.0771210950374327 6.867226498354952 >500 0.0044883514079508595 1.5470115204746613 >1k 0.001369327548185257 1.0068569672666254 >5k 1.5214750535391745E-4 0.5266813103816296 >10k+ 7.607375267695873E-5 0.406187650128623 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10612 0.40558322845319994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8477 0.32398501956254955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5283 0.20191257029007303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.821930158812664E-5 0.0 10-11 0.0 0.0 0.0 3.821930158812664E-5 0.0 12-13 0.0 0.0 0.0 7.643860317625328E-5 3.821930158812664E-5 14-15 0.0 0.0 0.0 7.643860317625328E-5 3.821930158812664E-5 16-17 0.0 0.0 0.0 1.1465790476437992E-4 7.643860317625328E-5 18-19 0.0 0.0 0.0 1.1465790476437992E-4 7.643860317625328E-5 20-21 0.0 0.0 0.0 1.7198685714656987E-4 7.643860317625328E-5 22-23 0.0 0.0 0.0 2.4842546032282313E-4 7.643860317625328E-5 24-25 0.0 0.0 0.0 5.159605714397096E-4 7.643860317625328E-5 26-27 0.0 0.0 0.0 0.001070140444467546 7.643860317625328E-5 28-29 0.0 0.0 0.0 0.003248640634990764 7.643860317625328E-5 30-31 0.0 0.0 0.0 0.011484900127232056 7.643860317625328E-5 32-33 0.0 0.0 0.0 0.029199546413328753 7.643860317625328E-5 34-35 0.0 0.0 0.0 0.055723741715488634 7.643860317625328E-5 36-37 0.0 0.0 3.821930158812664E-5 0.09824271473227952 7.643860317625328E-5 38-39 0.0 0.0 3.821930158812664E-5 0.15908784286057714 7.643860317625328E-5 40-41 0.0 0.0 3.821930158812664E-5 0.22331537917942396 7.643860317625328E-5 42-43 0.0 0.0 3.821930158812664E-5 0.28588037587918724 7.643860317625328E-5 44-45 0.0 0.0 3.821930158812664E-5 0.3648032336586688 7.643860317625328E-5 46-47 0.0 0.0 3.821930158812664E-5 0.4595871015972228 7.643860317625328E-5 48-49 0.0 0.0 3.821930158812664E-5 0.5562246056628011 1.1465790476437992E-4 50-51 0.0 0.0 3.821930158812664E-5 0.6601046673793293 1.5287720635250655E-4 52-53 0.0 0.0 3.821930158812664E-5 0.7593601936036941 1.5287720635250655E-4 54-55 0.0 0.0 3.821930158812664E-5 0.8693935628759107 1.5287720635250655E-4 56-57 0.0 0.0 3.821930158812664E-5 0.9933387579262054 1.5287720635250655E-4 58-59 0.0 0.0 3.821930158812664E-5 1.1231123964686893 1.5287720635250655E-4 60-61 0.0 0.0 3.821930158812664E-5 1.2664347774241642 1.5287720635250655E-4 62-63 0.0 0.0 3.821930158812664E-5 1.4042153596493607 1.9109650794063319E-4 64-65 0.0 0.0 3.821930158812664E-5 1.5439451262555517 1.9109650794063319E-4 66-67 0.0 0.0 3.821930158812664E-5 1.703606258639951 1.9109650794063319E-4 68-69 0.0 0.0 3.821930158812664E-5 1.883561840167645 1.9109650794063319E-4 70-71 0.0 0.0 3.821930158812664E-5 2.070091141568497 1.9109650794063319E-4 72-73 0.0 0.0 3.821930158812664E-5 2.257690583413817 2.102061587346965E-4 74-75 0.0 0.0 3.821930158812664E-5 2.4477933895131585 2.2931580952875985E-4 76-77 0.0 1.910965079406332E-5 3.821930158812664E-5 2.6562414603748015 2.2931580952875985E-4 78-79 0.0 5.732895238218996E-5 3.821930158812664E-5 2.8852324058400622 2.484254603228232E-4 80-81 0.0 9.55482539703166E-5 3.821930158812664E-5 3.1264917471151117 2.675351111168865E-4 82-83 0.0 1.1465790476437992E-4 3.821930158812664E-5 3.370197123691801 2.675351111168865E-4 84-85 0.0 1.1465790476437992E-4 3.821930158812664E-5 3.6151637372208985 3.2486406349907645E-4 86-87 0.0 1.1465790476437992E-4 3.821930158812664E-5 3.871061071004201 3.4397371429313974E-4 88-89 0.0 1.1465790476437992E-4 3.821930158812664E-5 4.143965993994218 3.4397371429313974E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6115 0.0 42.76204 1 GTATCAA 9255 0.0 39.524586 1 TATCAAC 11570 0.0 31.203234 2 ATCAACG 11655 0.0 30.894043 3 TCAACGC 11865 0.0 30.347246 4 CAACGCA 12025 0.0 29.903902 5 AACGCAG 12375 0.0 29.090439 6 CGCAGAG 14410 0.0 24.740808 8 ACGCAGA 14555 0.0 24.529343 7 TACATGG 8880 0.0 23.514875 2 GATCGGA 480 0.0 22.77953 7 GTACATG 9410 0.0 22.625616 1 GCAGAGT 15725 0.0 22.157541 9 GAGTACT 8835 0.0 21.871515 12-13 ACATGGG 9195 0.0 21.4678 3 ATCGGAC 455 0.0 20.894653 8 GTACTTT 9175 0.0 20.74916 14-15 AGTACTT 8980 0.0 20.353771 12-13 AGAGTAC 13890 0.0 19.779778 10-11 CAGAGTA 15070 0.0 19.445343 10-11 >>END_MODULE