FastQCFastQC Report
Thu 26 May 2016
SRR938220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2616479
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103360.395034701214877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87350.3338455993722862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.20393819327424373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.043.7239951
GTATCAA95550.036.3450431
ATCAACG117600.029.1589723
TCAACGC118050.029.004264
TATCAAC118700.028.973762
CAACGCA120100.028.5882665
AACGCAG123900.027.6731366
CGCAGAG143100.023.8610788
ACGCAGA146050.023.379127
GAGTACT89500.022.73719612-13
TAAGGTG11700.022.7297525
TACATGG85500.022.5567722
GTACATG89150.022.2214931
GTACTTT91050.022.14106814-15
GCAGAGT156400.021.4372339
GTAAGGT9900.021.10624
ACATGGG89050.020.640463
AGTACTT91450.020.17513312-13
CAGAGTA149150.020.0914410-11
GGACCGA8000.019.589196
Produced by FastQC (version 0.11.5)